BACKGROUND： Activated clotting factor VII has been demonstrated to exhibit obvious anti-apoptosis effects. OBJECTIVE： To observe the effect of activated clotting factor VII on neuronal apoptosis at different time po...BACKGROUND： Activated clotting factor VII has been demonstrated to exhibit obvious anti-apoptosis effects. OBJECTIVE： To observe the effect of activated clotting factor VII on neuronal apoptosis at different time points following rat intracerebral hemorrhage （ICH）. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Neurobiological Laboratory of Second Military Medical University from October 2005 to April 2006. MATERIALS： Recombinant-activated clotting factor Vlla （rFVtla） was purchased from Danish Novo Nordisk, Denmark. In situ cell death detection kit-POD kit was purchased from Roche, Switzerland. Caspase-3 activity determination kit from Biovision, USA. METHODS： A total of 72 healthy, male, Sprague Dawley rats, aged 5-8 months, were randomly assigned to three groups （n = 24）： sham-operated, ICH model, and rFVIla. In the ICH model and rFVIla groups, 80.0μL autologous non-clotting blood from rat tails was injected into the right caudate putamen to establish the ICH. The empty microinjector was inserted into the caudate putamen in the sham-operated group. The ICH model and rFVIla groups were subdivided into four subsets separately： 6, 24, 72 hours and 7 days following ICH. The rats in the rFVIla group were injected with 160 μg/kg rFVIla via the dorsal vein of the penis. MAIN OUTCOME MEASURES： Apoptotic cells were detected in the right caudate putamen by TUNEL; caspase-3 activity by spectrophotometry; and rat neurological function was evaluated by neurological functional impairment scales. RESULTS： Rat neurological function was deteriorated at 24, 72 hours, and 7 days following ICH. The TUNEL-positive cells and caspase-3 activity in the right caudate putamen was significantly increased in the ICH rats （P 〈 0.05）; rFVlla treatment reduced the number of TUNEL-positive cells and caspase-3 activity in the right caudate putamen （P 〈 0.05）, and neurological function was significantly improved （P 〈 0.05）. CONCLUSION： rFVIla was applied within 72 hours after tCH, which reduced the amount of neuronal apoptosis and promoted neurological function restoration by possibly inhibiting caspase-3 activity.展开更多
文摘BACKGROUND： Activated clotting factor VII has been demonstrated to exhibit obvious anti-apoptosis effects. OBJECTIVE： To observe the effect of activated clotting factor VII on neuronal apoptosis at different time points following rat intracerebral hemorrhage （ICH）. DESIGN, TIME AND SETTING： A randomized, controlled, animal experiment was performed at the Neurobiological Laboratory of Second Military Medical University from October 2005 to April 2006. MATERIALS： Recombinant-activated clotting factor Vlla （rFVtla） was purchased from Danish Novo Nordisk, Denmark. In situ cell death detection kit-POD kit was purchased from Roche, Switzerland. Caspase-3 activity determination kit from Biovision, USA. METHODS： A total of 72 healthy, male, Sprague Dawley rats, aged 5-8 months, were randomly assigned to three groups （n = 24）： sham-operated, ICH model, and rFVIla. In the ICH model and rFVIla groups, 80.0μL autologous non-clotting blood from rat tails was injected into the right caudate putamen to establish the ICH. The empty microinjector was inserted into the caudate putamen in the sham-operated group. The ICH model and rFVIla groups were subdivided into four subsets separately： 6, 24, 72 hours and 7 days following ICH. The rats in the rFVIla group were injected with 160 μg/kg rFVIla via the dorsal vein of the penis. MAIN OUTCOME MEASURES： Apoptotic cells were detected in the right caudate putamen by TUNEL; caspase-3 activity by spectrophotometry; and rat neurological function was evaluated by neurological functional impairment scales. RESULTS： Rat neurological function was deteriorated at 24, 72 hours, and 7 days following ICH. The TUNEL-positive cells and caspase-3 activity in the right caudate putamen was significantly increased in the ICH rats （P 〈 0.05）; rFVlla treatment reduced the number of TUNEL-positive cells and caspase-3 activity in the right caudate putamen （P 〈 0.05）, and neurological function was significantly improved （P 〈 0.05）. CONCLUSION： rFVIla was applied within 72 hours after tCH, which reduced the amount of neuronal apoptosis and promoted neurological function restoration by possibly inhibiting caspase-3 activity.
