[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid rati...[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin. [Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests. Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively. [Results] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution (pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1:30. In addition,pumpkin polysaccharides had a strong activity of eliminating ·OH,but very weak activity to scavenge O-2. [Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide.展开更多
Carboxymethylpachyman(Ⅰ)was formed by carboxymethylation of β-pachyman.The antitumour activity of carboxymethylpachyman(Ⅰ)against S_(180)、EAC、 MA、U_(14)was measured.The structure of carboxymethylpachyman(Ⅰ)was ...Carboxymethylpachyman(Ⅰ)was formed by carboxymethylation of β-pachyman.The antitumour activity of carboxymethylpachyman(Ⅰ)against S_(180)、EAC、 MA、U_(14)was measured.The structure of carboxymethylpachyman(Ⅰ)was proved by IR ^(13)CNMR spectroscopy.展开更多
基金Supported by the Key Scientific and Technological Project of Henan Province (102102110157)the Scientific Research Found Project of Henan University of Urban Construction (2010JZD008)~~
文摘[Objective] This paper aims to study a new method of extracting pumpkin polysaccharide from pumpkin. Single factor experiments were conducted to examine the effects of extracting time,temperature,the solid-liquid ratio and pH value on the extraction yield of polysaccharide from pumpkin. [Method] The best enzyme ratio and extraction conditions for complex enzymes extraction were determined through orthogonal tests. Scavenging ·OH and O-2 activities of pumpkin polysaccharides were also investigated by salicylic acid and improved self-oxidation of o-pheno methods respectively. [Results] The results showed that the biggest extraction yield of polysaccharide from pumpkin can be got when adding 1% cellulose enzyme,1.5% pectinase,1.0% papain and Na2HPO4-citric acid buffer solution (pH was 4.6),and oscillating for 30 min under water at 40 ℃ with the solid-liquid ratio of 1:30. In addition,pumpkin polysaccharides had a strong activity of eliminating ·OH,but very weak activity to scavenge O-2. [Conclusion] This study provided basic data for research and application of Pumpkin polysaccharide.
文摘Carboxymethylpachyman(Ⅰ)was formed by carboxymethylation of β-pachyman.The antitumour activity of carboxymethylpachyman(Ⅰ)against S_(180)、EAC、 MA、U_(14)was measured.The structure of carboxymethylpachyman(Ⅰ)was proved by IR ^(13)CNMR spectroscopy.
