Effects of Long-term Located Fertilization on Evolution of Available Phosphorus and Phosphorus Pool in Shandong Fluvo-aquic Soil

This study was conducted to investigate the effects of long-term located fertilization on soil phosphorus,the changes of soil available phosphorus（ OlsenP）,the evolution of soil total phosphorus（ TP） and the ratio change of Olsen-P to TP（ PAC） by 33-year fertilization experiments in winter wheat-summer maize rotation system in Shandong fluvo-aquic soil. Eight treatments were designed as no fertilization（ CK）,nitrogen fertilizer（ N）,nitrogen and phosphate fertilizer（ NP）,nitrogen and potassium fertilizer（ NK）,phosphate and potassium fertilizer（ PK）,nitrogen-phosphate-potassium fertilizer（ NPK）,reduced NPK fertilizer（ N（15） PK）,and increased NPK fertilizer（ N（25） PK）. Meanwhile,eight organic fertilizer-added treatments were designed based on the application of inorganic fertilizer the same as the above ones. The results showed that TP,Olsen-P and PAC of treatments added with organic fertilizer were higher than those without organic fertilizer,and those of the treatments applied with phosphate fertilizer were higher than those of no phosphate fertilizer. With the increase of years,soil P pool decreased due to crop absorption,nutrient loss and morphological transformation and other causes under the treatments of without and only phosphate fertilizer,while remained stable under the treatments added with organic fertilizer. The PAC values were generally lower in fluvo-aquic soil,and it could be improved by the application of organic fertilizer. On the whole,the application of chemical phosphate fertilizer combined with organic fertilizer could improve the phosphorus content in soil and ensure the supply of phosphorus nutrition. This study would provide scientific basis for fertilization management and soil fertility in fluvo-aquic soil.

Influence of phosphorus availability on the community structure and physiology of cultured biofilms

Biofilms have important effects on nutrient cycling in aquatic ecosystems.However,publications about the community structure and functions under laboratory conditions are rare.This study focused on the developmental and physiological properties of cultured biofilms under various phosphorus concentrations performed in a closely controlled continuous flow incubator.The results showed that the biomass（Chl a）and photosynthesis of algae were inhibited under P-limitation conditions,while the phosphatase activity and P assimilation rate were promoted.The algal community structure of biofilms was more likely related to the colonization stage than with the phosphorus availability.Cyanobacteria were more competitive than other algae in biofilms,particularly when cultured under low P levels.A dominance shift occurred from non-filamentous algae in the early stage to filamentous algae in the mid and late stages under P concentrations of 0.01,0.1 and 0.6 mg/L.However,the total N content,dry weight biomass and bacterial community structure of biofilms were unaffected by phosphorus availability.This may be attributed to the low respiration rate,high accumulation of extracellular polymeric substances and high alkaline phosphatase activity in biofilms when phosphorus availability was low.The bacterial community structure differed over time,while there was little difference between the four treatments,which indicated that it was mainly affected by the colonization stage of the biofilms rather than the phosphorus availability.Altogether,these results suggested that the development of biofilms was influenced by the phosphorus availability and/or the colonization stage and hence determined the role that biofilms play in the overlying water.