Teaching and research activities are of great significance to the professional development of teachers.The basic psychological needs of teachers include autonomy,competence,and attribution.The engagement of teachers i...Teaching and research activities are of great significance to the professional development of teachers.The basic psychological needs of teachers include autonomy,competence,and attribution.The engagement of teachers in teaching and research activities can be analyzed from these three perspectives.Under the management system of teaching and research,teachers generally lack autonomy in the organization of teaching and research activities;they are also lack scientific research knowledge and ability;in addition,under the influence of harmonious interpersonal relationships and the culture of individualism,there is a dearth in real communication among teachers.In light of this,we propose targeted countermeasures to promote the vitality of teaching and research activities.展开更多
AIM: To investigate the role of the mitochondrial pathway in JTE-522-induced apoptosis and to investigate the relationship between cytochrome C release, caspase activity and loss of mitochondrial membrane potential (D...AIM: To investigate the role of the mitochondrial pathway in JTE-522-induced apoptosis and to investigate the relationship between cytochrome C release, caspase activity and loss of mitochondrial membrane potential (Deltapsim). METHODS: Cell culture, cell counting, ELISA assay, TUNEL, flow cytometry, Western blot and fluorometric assay were employed to investigate the effect of JTE-522 on cell proliferation and apoptosis in AGS cells and related molecular mechanism. RESULTS: JTE-522 inhibited the growth of AGS cells and induced the apoptosis. Caspases 8 and 9 were activated during apoptosis as judged by the appearance of cleavage products from procaspase and the caspase activities to cleave specific fluorogenic substrates. To elucidate whether the activation of caspases 8 and 9 was required for the apoptosis induction, we examined the effect of caspase-specific inhibitors on apoptosis. The results showed that caspase inhibitors significantly inhibited the apoptosis induced by JTE-522. In addition, the membrane translocation of Bax and cytosolic release of cytochrome C accompanying with the decrease of the uptake of Rhodamin 123, were detected at an early stage of apoptosis. Furthermore, Bax translocation, cytochrome C release, and caspase 9 activation were blocked by Z-VAD.fmk and Z-IETD-CHO. CONCLUSION: The present data indicate a crucial association between activation of caspases 8, 9, cytochrome C release, membrane translocation of Bax, loss of Deltapsim and JTE-522-induced apoptosis in AGS cells.展开更多
提升STEM教育质量,离不开STEM课程的创新设计。研究性学习与STEM课程的有机整合,逐步成为STEM课程建设的重要趋势。为挖掘研究性学习与STEM课程整合的内在逻辑,文章以美国“STEM Student Research Handbook”一书为分析对象,从教学内容...提升STEM教育质量,离不开STEM课程的创新设计。研究性学习与STEM课程的有机整合,逐步成为STEM课程建设的重要趋势。为挖掘研究性学习与STEM课程整合的内在逻辑,文章以美国“STEM Student Research Handbook”一书为分析对象,从教学内容、教学活动和教学评价三方面分析了该教材在指导学生STEM科学研究方面的特色,研究结果表明:该教材关注科学研究知识的系统性、强调以设计研究引导教学活动、借助信息技术提升研究效率、注重对学生多元学习证据的评价。基于此,文章从多元知识体系奠基、系统研究流程驱动、伴随性评价引导三个方面,论述了该教材对我国STEM课程建设的启示,以期为我国STEM课程的建设提供参考,并助推我国STEM课程与研究性学习的深度融合。展开更多
English language activities are enjoyable and respect the students’subjectivity,which stimulates their enthusiasm for using English communication.Therefore,it is widely welcomed by junior high school students.The dev...English language activities are enjoyable and respect the students’subjectivity,which stimulates their enthusiasm for using English communication.Therefore,it is widely welcomed by junior high school students.The development of the English club and their activities,such as membership,management systems,event planning,advisory lecturers,and cooperation with school management,among others,all directly affect the club activities.Therefore,it is essential to promote their club activities in junior high school English language learning.This paper analyzes the role of the English club and explores their process of conducting specific activities.It is hoped that this paper will be used as a reference for future studies.展开更多
文摘Teaching and research activities are of great significance to the professional development of teachers.The basic psychological needs of teachers include autonomy,competence,and attribution.The engagement of teachers in teaching and research activities can be analyzed from these three perspectives.Under the management system of teaching and research,teachers generally lack autonomy in the organization of teaching and research activities;they are also lack scientific research knowledge and ability;in addition,under the influence of harmonious interpersonal relationships and the culture of individualism,there is a dearth in real communication among teachers.In light of this,we propose targeted countermeasures to promote the vitality of teaching and research activities.
基金National Natural Science Foundation of China,No.39770300,30070873the Overseas Chinese Affairs Office of the State Council Foundation,No.98-33
文摘AIM: To investigate the role of the mitochondrial pathway in JTE-522-induced apoptosis and to investigate the relationship between cytochrome C release, caspase activity and loss of mitochondrial membrane potential (Deltapsim). METHODS: Cell culture, cell counting, ELISA assay, TUNEL, flow cytometry, Western blot and fluorometric assay were employed to investigate the effect of JTE-522 on cell proliferation and apoptosis in AGS cells and related molecular mechanism. RESULTS: JTE-522 inhibited the growth of AGS cells and induced the apoptosis. Caspases 8 and 9 were activated during apoptosis as judged by the appearance of cleavage products from procaspase and the caspase activities to cleave specific fluorogenic substrates. To elucidate whether the activation of caspases 8 and 9 was required for the apoptosis induction, we examined the effect of caspase-specific inhibitors on apoptosis. The results showed that caspase inhibitors significantly inhibited the apoptosis induced by JTE-522. In addition, the membrane translocation of Bax and cytosolic release of cytochrome C accompanying with the decrease of the uptake of Rhodamin 123, were detected at an early stage of apoptosis. Furthermore, Bax translocation, cytochrome C release, and caspase 9 activation were blocked by Z-VAD.fmk and Z-IETD-CHO. CONCLUSION: The present data indicate a crucial association between activation of caspases 8, 9, cytochrome C release, membrane translocation of Bax, loss of Deltapsim and JTE-522-induced apoptosis in AGS cells.
文摘提升STEM教育质量,离不开STEM课程的创新设计。研究性学习与STEM课程的有机整合,逐步成为STEM课程建设的重要趋势。为挖掘研究性学习与STEM课程整合的内在逻辑,文章以美国“STEM Student Research Handbook”一书为分析对象,从教学内容、教学活动和教学评价三方面分析了该教材在指导学生STEM科学研究方面的特色,研究结果表明:该教材关注科学研究知识的系统性、强调以设计研究引导教学活动、借助信息技术提升研究效率、注重对学生多元学习证据的评价。基于此,文章从多元知识体系奠基、系统研究流程驱动、伴随性评价引导三个方面,论述了该教材对我国STEM课程建设的启示,以期为我国STEM课程的建设提供参考,并助推我国STEM课程与研究性学习的深度融合。
文摘English language activities are enjoyable and respect the students’subjectivity,which stimulates their enthusiasm for using English communication.Therefore,it is widely welcomed by junior high school students.The development of the English club and their activities,such as membership,management systems,event planning,advisory lecturers,and cooperation with school management,among others,all directly affect the club activities.Therefore,it is essential to promote their club activities in junior high school English language learning.This paper analyzes the role of the English club and explores their process of conducting specific activities.It is hoped that this paper will be used as a reference for future studies.
基金supported by the project of Research on Educating Excellent Foreign Language Teachers in the New Era supported by China National Social Science Foundation (19BYY220)。
