Salivary alpha-amylase activity—An indicator of relaxation response in perioperative patients

The psychological stress of most perioperative patients has been reduced by many interventions. In order to evaluate the effects of these interventions, biomarkers are being considered as objective assessment tools in addition to the subjective assessment tools. It has been thought that salivary α-amylase (sAA) activity is associated with sympathetic nervous system activity, which reflects psychological stress. We examined the relationship between sAA, self-reported data, and autonomic nervous system activity to assess the reliability of sAA as a marker for relaxing response in surgery-related stress. The sAA, the State anxiety, Visual Analogue Scale (VAS) of pain, and heart rate variability were used to assess prior to and after the relaxation on the day before surgery, and on postoperative days (POD) 1, 2, 3, and 7. A total of 75 patients participated and 301 relaxing response readings were obtained. We found that the sAA significantly decreased depending upon the pain and anxiety by relaxation, but they were unrelated to sympathetic nervous system activity. There was weak correlation between the change in sAA, and anxiety and VAS pain. Thus, sAA appears to be an easy-to-use, non-invasive and good marker for relaxing response within a short period in surgery-related stress patients.

Synthesis and Characterization of Zinc Oxide and Zinc Oxide Doped with Chlorine Nanoparticles as Novel <i>α</i>-Amylase Inhibitors

In this study we used a chemical solution method from oxalic acid (OX. acid) and zinc acetate (ZnAc) to prepare Zinc Oxide nanoparticles (ZnONPs) and Zinc Oxide nanoparticles doped with Chlorine (Cl:ZnONPs). The characterizations (FTIR, X-ray, SEM, TEM) of ZnONPs and Cl:ZnONPs were determined. Amylase inhibitors of ZnONPs and Cl:ZnONPs also were determined. SEM indicated that the ZnONPs and Cl:ZnONPs have an average particle size of 46.65 - 74.64 nm. TEM images of the ZnONPs and Cl:ZnONPs showed the round shaped. Compounds b, d and e exhibited significant inhibitory activity against amylase enzyme (from 69.21 ± 1.44 to 76.32 ± 0.78), respectively, and were comparable with that of acarbose (86.32 ± 0.63) at 1000 μg, thereby, projecting ZnONPs and Cl:ZnONPs as α-amylase inhibitors.

补肾序贯结合活血化瘀方治疗子宫内膜异位症所致痛经的临床疗效及对CA125、PGF_(2α)、OT的影响

目的:探讨补肾序贯结合活血化瘀中药对子宫内膜异位症所致痛经的临床疗效及对糖类抗原CA125、前列腺素(PGF_(2α))、催产素(OT)的影响。方法:将子宫内膜异位症痛经患者随机分成补肾序贯结合活血化瘀中药组(治疗组),中成药活血化瘀组(对照组),其中治疗组50例,对照组30例,观察治疗前后2组患者痛经改善情况,比较治疗前后2组患者血清CA125、PGF_(2α)、OT水平的变化。结果:治疗组治疗痛经总有效率为96%,对照组治疗痛经总有效率为86.6%,两者总有效率差异有统计学意义(P<0.05)。2组治疗后痛经程度评分及兼症改善评分均较治疗前差异有统计学意义(P<0.05),其中治疗组治疗后的兼症评分下降程度与对照组比较差异有统计学意义(P<0.05);治疗后2组患者血清CA125、PGF_(2α)、OT含量均较治疗前明显下降(P<0.05),比较治疗组的效果更加明显,其中对CA125及OT水平的影响与对照组比较差异有统计学意义(P<0.05)。结论:活血化瘀方治疗子宫内膜异位症痛经患者,可以明显改善患者的痛经和兼症症状,降调患者经期血清中CA125、PGF_(2α)、OT等水平,而与补肾序贯法结合使用则疗效更显著,推断中药活血化瘀方治疗内异症的作用机制可能与降调上述指标有关,而补肾序贯中药可能具有免疫调控机制,能够改善痛经患者内分泌环境,不利于异位内膜的黏附,侵袭,从其作用靶点即"源头"上遏制而显疗效。

揿针联合站立数字作业治疗对卒中后肩痛的临床研究

目的:观察揿针联合站立数字作业治疗(OT)对卒中后肩痛的疗效。方法:将64例卒中后肩痛患者随机分为对照组和观察组各32例,均进行为期4周、每周5d的常规治疗;对照组在常规治疗基础上进行揿针治疗,主穴取患侧肩贞、臑俞、天宗、秉风、曲垣、肩外俞、肩中俞,配穴取合谷、中渚、后溪,留针4h后取下,1次/d;观察组在对照组的基础上增加站立数字OT训练,20 min/次,1次/d。在治疗前、治疗4周后均采用视觉模拟量表(VAS)评分、简化Fugl-Meyer运动功能评定表上肢部分(FMA-UE)及改良Barthel指数(MBI)分别评定肩痛程度、上肢运动功能及日常生活能力。结果:与治疗前相比,治疗4周后2组的VAS评分均显著降低、FMA-UE和MBI评分均显著提高(均P<0.05);与对照组相比,观察组治疗4周后的VAS评分显著降低、FMA-UE和MBI评分均显著提高(均P<0.05)。结论:揿针联合站立数字OT训练可有效减轻卒中后肩痛患者的肩部疼痛、改善上肢运动功能并提高日常生活能力。

靳三针联合站立数字OT训练对脑卒中偏瘫患者上肢运动功能的影响

目的:观察靳三针疗法结合站立数字OT训练对脑卒中后偏瘫患者上肢运动功能的影响。方法:选择2021年1—6月在广东省第二中医院针灸康复科住院部治疗的卒中后上肢偏瘫患者60例,采用随机数字表法分为对照组和观察组,每组30例。2组均接受常规药物治疗和常规康复训练;对照组在常规治疗基础上采用靳三针治疗,选择患肢对侧颞三针、患肢肩三针、手三针(曲池、外关、合谷)、百会、水沟穴进行针刺,以平补平泻手法施针,以患者得气为度,留针30 min,1次/d,6次/周,持续治疗8周;观察组在对照组的基础上接受站立数字OT训练,20 min/次,1次/d,6次/周,持续治疗8周。在干预前、干预8周后采用Fugl-Meyer运动功能评定表上肢部分(FMA-UE)评定上肢运动功能;采用Wolf上肢运动功能评价量表(WMFT)评定上肢运动完成量;采用改良Barthel指数(MBI)评定日常生活活动能力。结果:与治疗前比较,2组治疗后FMA-UE、WMFT、MBI评分均明显升高,差异均具有统计学意义(P<0.05)。与对照组比较,观察组治疗后FMA-UE、WMFT、MBI评分均明显更高,差异均具有统计学意义(P<0.05)。结论:靳三针联合站立数字OT训练能有效地改善脑卒中偏瘫患者上肢运动功能和日常生活活动能力。

Rate of pozzolanic reaction of two kinds of activated coal gangue

Two kinds of activated ways are used to prepare activated coal gangue fine powder,one is calcining coal gangue at 800(gangue A),and the other is calcining coal gangue with a certain calcite at 800(gangue B).The experiment shows that strengths of blended cement mortar with coal gangue B are higher than that of blended cement with coal gangue A.Hydration of cements with the two kinds of activated coal gangue is investigated through a differential thermal analysis.The weight loss due to Ca(OH)2 decomposition of hydration products by differential thermal analysis/thermo gravimetric(DTA/TG) can be used to quantify the pozzolanic reaction.A new method based on the composition of hydration cement is proposed to determine the degree of pozzolanic reaction.The results obtained suggest that the degree of pozzolanic reaction of gangue B is faster than that of gangue A.

数字OT认知功能训练对脑卒中注意功能下降患者康复效果观察

目的:探讨数字OT认知功能训练对脑卒中注意功能下降患者注意能力及日常生活活动能力的影响。方法:将50例脑卒中后注意功能下降患者按照随机数字表法分为观察组24例和对照组26例。观察组给予数字OT认知功能训练,对照组接受常规认知功能训练,每次20 min,每天1次,每周训练6 d,连续8周。治疗前后采用蒙特利尔认知评定量表(Mo CA)、简单反应时、字母划销测验评价2组患者注意能力,Barthel指数评价其日常生活活动能力。结果:治疗8周后,2组Mo CA注意评分、Barthel指数评分较治疗前明显增加(P<0.05),简单反应时较治疗前明显缩短(P<0.05),字母划销测验中遗漏数、错误数及完成时间较治疗前明显减少(P<0.05)。治疗后2组上述指标组间比较,差异有统计学意义(P<0.05)。结论:数字OT认知功能训练能有效提高脑卒中注意功能下降患者的注意能力和生活自理能力,其疗效优于常规认知功能训练,值得临床推广。

Effect of static magnetic field on activity of immobilized α-amylase

BIOLOGICAL effect of magnetic field is one of the three major subjects of contemporary biomagnetism, and is also the basis of applied biomagnetism. In order to explore magnetic biological effects, the influence of various sorts of magnetic field on many living systems as well as their different constructural levels has been extensively studied. However, only a few studies with in vitro enzyme have been reported, and few researches concerning the influence of magnetic field on immobilized enzyme reactions have been published. This note describes

Effect of Moisture Content on Melilotus suaveolens Seed Quality During Ultra-drying Storage

In order to enhance the seed storability and supply high-quality seeds for vegetation restoration in the arid and semi-arid regions in Northwest China,the effects of ultra-drying and accelerated aging on the physiological characterstics of Melilotus suaveolens seeds were studied.Melilotus suaveolens seeds were dried in a desiccator containing silica gel to 80,53,42,33,23 and 16 g·kg-1 of moisture contents(MC),respectively.The parameters of the seed quality including germination energy(GE),germination percentage(GP),relative conductivity(RC),dehydrogenase activity(DA) and α-amylase activity(AA) were determined after ultra-drying and accelerated aging.The results showed that ultra-dried seeds with 42 g·kg-1 of MC showed the minimum changes of GE and GP before and after seed aging.Moreover,ultra-dry seeds with 42 g·kg-1 of MC showed higher DA and AA,and lower RC than non-ultra-drying seeds.Therefore,ultra-drying to 42 g·kg-1 of MC was helpful for M.suaveolens seed storage.

11th Panchen Erdeni:Good at Buddhist Activities

At 18:30 on the evening of June 15, horns were blown as the 11th Panchen Erdeni stepped out of the Sunlight Hall of the Tashilhungpo Monastery in Xigaze into the Dorgyia Courtyard, where he was seated on the throne prepared for him.When all of the 500-odd monks present had taken their own seats, the young Buddhist master led them to recite the Sutra of Tara and eight other sutras.The sutra recitation lasted about 90 minutes. The young master, seated on his throne from beginning to end, never stumbled while the monks recited with closed eyes and lowered heads, obvi-

壳聚糖对小麦种子萌发及幼苗生理生化特性的影响

用不同浓度的壳聚糖溶液包衣处理“8802”和“徐州-24”两个小麦品种,在20℃下发芽,并测定种子发芽率、α-淀粉酶活性及幼苗的湿重、干重、叶绿素、可溶性糖和可溶性蛋白质等的含量。结果表明:壳聚糖处理后,能够有效地提高小麦种子发芽率、α-淀粉酶活性、幼苗湿重、干重及幼苗叶片中叶绿素、可溶性糖、可溶性蛋白质等的含量。

结构离复位控制的可行性研究

发展了一种离复位控制策略,通过考虑与模糊控制理论相结合实施结构振动控制。这种控制方法不仅体现了模糊控制系统无需精确数学模型即可有效执行控制功能,具有反应迅速、超调量小和鲁棒性好等诸多优点,而且解决了其控制规则不易建立的难题。采用这种控制策略,研究了一个底层设有主动拉索控制系统的多层结构的地震反应控制问题。在算例中通过与线性二次型最优控制算法进行控制效果的对比,验证了它的可行性。

半主动U型液力阻尼器的结构振动控制

半主动控制系统由于其动力可调范围广、耗能小和内在稳定性好等特点 ,受到了普遍的关注。基于离复位控制 (OTE)算法 ,本文检验了一变阻尼半主动控制装置 -SATLCD的风振控制效果。本文的主要目标是研究OTE算法应用于SATLCD实施结构振动控制的可能性。数值研究确认了SATLCD的有效性和它相对于无控和被动控制的优越性 。

脊髓TLR4/p38MAPK级联活化介导持续性术后痛时TNF-α的增加

目的:研究脊髓肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)在持续性术后痛中的作用及与Toll样受体4(TLR4)/p38有丝分裂原活化蛋白激酶(p38MAPK)级联的关系。方法:选择鞘内置管成功的雄性SD大鼠120只,采用随机数字表法随机分为5组(每组24只):假手术组、持续性术后痛组、Toll样受体4小干扰RNA组(TLR4 si RNA组)、4-(4-氟苯基)-2-(4-甲磺酰基苯基)-5-(4-吡啶基)咪唑组(SB203580组)和重组人Ⅱ型肿瘤坏死因子组(rh TNFR-Fc组)。按Flatters介绍的方法建立大鼠皮肤肌肉切口牵拉(skin/muscle incision and retraction,SMIR)持续性术后痛模型;在术前1天及术后第1、3、7、12和22天时测定大鼠机械缩足反应阈值(mechanical withdrawal threshold,MWT);按ELISA法测定脊髓TNF-α含量。结果:与术前基础值及假手术组比较,持续性术后痛组大鼠在术后第3、7、12和22天MWT明显降低(P<0.05),脊髓TNF-α含量在术后7、12和22天明显升高(P<0.05);与持续性术后痛组比较,TLR4 si RNA组和SB203580组大鼠在术后第3、7和12天,rh TNFR-Fc组在术后第7和12天MWT明显升高(P<0.05),TLR4 si RNA组、SB203580组和rh TNFR-Fc组大鼠脊髓TNF-α含量在术后第7、12和22天明显降低(P<0.05)。结论:脊髓TNF-α参与了大鼠SMIR持续性术后痛的形成,其增加与脊髓TLR4/p38MAPK级联的活化有关。

内皮—单核细胞激活多肽酶Ⅱ增强血肿瘤屏障通透性的作用位点

目的研究内皮—单核细胞激活多肽Ⅱ(EMAP-Ⅱ)增强血肿瘤屏障(BTB)的作用机制。方法采集出生3～5 d的Wistar胎鼠大脑皮质,应用酶消化法及葡聚糖离心法获得脑微血管段后,接种于培养皿中进行脑微血管内皮细胞(BMEC)的原代培养;将BMEC与C6脑胶质瘤细胞共培养,构建体外BTB模型;实验随机分为4组(每组6例):对照组、EMAP-Ⅱ组、寡霉素组和寡霉素+EMAP-Ⅱ组。测定跨内皮阻抗值和辣根过氧化物酶流量,评估各组BTB通透性变化;Western blot检测BMEC上紧密连接(TJ)相关蛋白ZO-1的表达水平;双重免疫荧光法检测EMAP-Ⅱ和α-ATP合成酶在BMEC上的分布。结果 EMAP-Ⅱ组BTB通透性较对照组和寡霉素组显著增高(P<0.01);与对照组和寡霉素组比较,EMAP-Ⅱ组TJ相关蛋白ZO-1的表达水平显著降低(P<0.01);EMAP-Ⅱ组与寡霉素组比较,EMAP-Ⅱ的作用受到ATP合成酶抑制剂寡霉素的显著抑制(P<0.01);EMAP-Ⅱ与α-ATP合成酶在BMEC膜上共定位。结论 EMAP-Ⅱ通过开放TJ增强BTB的通透性,BMEC膜上的α-ATP合成酶是其作用的位点。

我国国有商业银行表外业务发展的新思考——基于范围经济的分析框架

范围经济是对经济组织业务多元化选择与判断的衡量标准。商业银行作为金融企业,其范围经济的大小决定了它实行多元化经营以节约成本、提高效益的空间与机会。对我国国有商业银行表外业务范围经济的实证结果表明,我国四大国有商业银行在近年来大多数年份存在较为明显的范围经济,表外业务的开展对传统业务具有巨大的促进作用,有利于银行总体收入的增长。因此,国有商业银行应该大力开展表外业务,以充分发挥其范围经济的最大效益。

RANKL-RANK-OPG信号通路对类风湿关节炎骨破坏作用的研究进展

类风湿关节炎(RA)是一种以对称性、多关节的慢性炎症为主要特征的全身性自体免疫病,可导致软骨和骨骼的结构破坏。延缓甚至抑制RA患者骨破坏发生是临床治疗的主要目标。现有研究表明,在RA发病过程中,破骨细胞生成、分化和活化的改变在RA骨破坏的发病机制中起着至关重要的作用,RANKL-RANK-OPG信号系统是RA炎症导致继发性骨丢失的主要通路。但是,在RA骨破坏的过程中,RANKL-RANK-OPG系统与骨代谢系统间复杂的交互作用仍然有待进一步阐明。本文主要综述目前RANKL-RANK-OPG信号通路在RA骨破坏发生机制中的研究进展,以期为RA骨破坏的研究提供更系统的思路和新的方向。

结构离复位控制直接法的研究

针对传统控制算法通常难于处理结构控制系统在地震激励下的非线性行为、时变特征和参数不确定性这一难题,提出了一种基于离复位控制策略的直接法,可在相平面上通过网格划分设计控制力,这种控制方法可用于不同的控制系统．同时,通过数值算例分析了一个各层均设有主动拉索控制系统的3层剪切型结构的地震反应控制问题．研究结果表明,直接法的控制效果略优于LQR,而且无需建立控制系统的精确模型就可有效实施控制,验证了它的可行性和有效性．

义乌礼俗活动与礼俗音乐的考察与研究

义乌,不仅在商场上以"义乌经济模式"享誉国内外,为人类社会创造了丰富的物质财富,而且在历史发展的长河中,还延续和创造了丰富独特的民间文化艺术。本文以义乌礼俗音乐研究为出发点,旨在为这千年流传下来的礼俗活动及音乐的遗存工作做出努力,实现社会文化大繁荣景象。

对财政本质的再认识

我国对财政本质的传统论述中占主导地位的是“国家分配论”,随着对西方财政理论的研究和借鉴,现在比较具有代表性的观点认为财政是一种公共经济活动。但根据我国社会主义市场经济改革的实践,根据对市场与政府职能的再认识,财政是政府在生产、分配、交换、消费各环节的有计划的经济活动,可简称为“政府经济活动”。