Hypoxia-inducible factor 1 （HIF-1） attenuates amyloid-beta protein neurotoxicity and decreases apoptosis induced by oxidative stress or hypoxia in cortical neurons. In this study, we construct-ed a recombinant adeno...Hypoxia-inducible factor 1 （HIF-1） attenuates amyloid-beta protein neurotoxicity and decreases apoptosis induced by oxidative stress or hypoxia in cortical neurons. In this study, we construct-ed a recombinant adeno-associated virus （rAAV） vector expressing the human HIF-1αgene （rAAV-HIF-1α）, and tested the assumption that rAAV-HIF-1αrepresses hippocampal neuronal apoptosis induced by amyloid-beta protein. Our results conifrmed that rAAV-HIF-1αsigniifcant-ly reduces apoptosis induced by amyloid-beta protein in primary cultured hippocampal neurons. Direct intracerebral rAAV-HIF-1αadministration also induced robust and prolonged HIF-1αproduction in rat hippocampus. Single rAAV-HIF-1αadministration resulted in decreased apoptosis of hippocampal neurons in an Alzheimer＇s disease rat model established by intrace-rebroventricular injection of aggregated amyloid-beta protein （25-35）. Our in vitro and in vivo ifndings demonstrate that HIF-1 has potential for attenuating hippocampal neuronal apoptosis induced by amyloid-beta protein, and provides experimental support for treatment of neurode-generative diseases using gene therapy.展开更多
Objective:To compare effect of intrathecal meperidine,tramadol,magnesium sulfate,and dexmedetomidine on the prevention of post-spinal anesthesia shivering and adverse events in hip fracture repair patients.Methods:In ...Objective:To compare effect of intrathecal meperidine,tramadol,magnesium sulfate,and dexmedetomidine on the prevention of post-spinal anesthesia shivering and adverse events in hip fracture repair patients.Methods:In a randomized,double-blind trial,132 patients with American Society of Anesthesiology(ASA)ⅠandⅡspinal anesthesia who needed hip fracture surgery were enrolled.Patients were stratified into 4 intervention groups based on a randomized block pattern:meperidine,tramadol,magnesium sulfate,and dexmedetomidine.Hemodynamic parameters including blood pressure,heart rate,and oxygen saturation,as well as the severity of shivering,core body temperature,Ramsay sedation score,adverse events,meperidine consumption were recorded and compared.Results:There was no statistically significant difference in the normal hemodynamic parameters,temperature,duration of surgery,meperidine consumption,and adverse events such as dizziness,hypotension,nausea,and bradycardia among groups(P>0.05).Compared to other groups,severity of shivering was the lower in the dexmedetomidine group 6 and 8 h after surgery.The Ramsay sedation scores were higher in the dexmedetomidine and meperidine groups 4 h after surgery(P=0.020).Conclusion:Dexmedetomidine acts better than the other three adjuvants in reducing complications such as shivering.Overall,these four adjuvants are helpful to prevent postoperative shivering and could be put forward as promising local anesthetics in spinal anesthesia,based on anesthesiologists’discretion and patients’general conditions.Clinical registration:The study was approved by the Research and Ethics Committee at the Valiasr Hospital(Arak,Iran)with the clinical trial code of IRCT20141209020258N153.展开更多
OBJECTIVE: This study used gated myocardial perfusion imaging(G-MPI) to assess the clinical value of Xinmailong injection in chronic congestive heart failure(CHF).METHODS: A total of 102 CHF patients were randomly div...OBJECTIVE: This study used gated myocardial perfusion imaging(G-MPI) to assess the clinical value of Xinmailong injection in chronic congestive heart failure(CHF).METHODS: A total of 102 CHF patients were randomly divided into the control group(n=51) and the Xinmailong group(n=51). Patients in the control group were routinely treated. Patients in the Xinmailong group were additionally treated with Xinmailong injection in addition to routine treatment. Before and 3 months after treatment, G-MPI was used to determine changes in the left ventricular ejection fraction(LVEF), left ventricular end-diastolic volume(LVEDV) and left ventricular end-systolic volume(LVESV). Fourteen days after treatment, changes in plasma brain natriuretic peptide(BNP) levels were determined.RESULTS: Before treatment, there were no significant differences in LVEF, LVEDV, LVESV, and BNP levels between the two groups(all P>0.05). After treatment, LVEDV, LVESV, and BNP levels were significantly lower, and LVEF was significantly higher in the Xinmailong group than in the control group(all P<0.05).CONCLUSION: Additional use of Xinmailong injection in addition to routine treatment improves cardiac function of CHF patients. Because of the safety and effectiveness of Xinmailong injection, this therapy should be promoted.展开更多
Based on spin-flip model （SFM）, the nonlinear dynamics of 1550nm vertical-cavity surface-emitting lasers （VCSELs） subject to polarization-preserved optical feedback （PPOF） and orthogonal optical injection （OOI...Based on spin-flip model （SFM）, the nonlinear dynamics of 1550nm vertical-cavity surface-emitting lasers （VCSELs） subject to polarization-preserved optical feedback （PPOF） and orthogonal optical injection （OOI） are theoretically investigated. The results show that two linear polarization （LP） modes can be simultaneously stimulated and polarization switching （PS） can be observed, which is different from the case that only y LP mode exists in a VCSEL with PPOF. Under the joint action of PPOF and OOI, the two LP modes will exhibit rich dynamical states, such as period one, period two, multi- period and chaos oscillation. Different evolution routes to chaos can be also observed. Moreover, frequency detuning Af（Af=fm fs, wherefn andfs are the center frequencies of free-running master VCSEL and slave VCSEL, respectively） has an obvious influence on the PS. With the increase of the positive frequency detuning PS points shift toward larger injection strengths; meanwhile, a suitable negative frequency demning value makes the injection strength for PS be the lowest.展开更多
基金supported by the National Natural Science Foundation of China,No.81273983the Natural Science Foundation of Hebei Province of China,No.C2010001471the Scientific Research Fund of Hebei Provincial Education Department in China,No.Q2012036
文摘Hypoxia-inducible factor 1 （HIF-1） attenuates amyloid-beta protein neurotoxicity and decreases apoptosis induced by oxidative stress or hypoxia in cortical neurons. In this study, we construct-ed a recombinant adeno-associated virus （rAAV） vector expressing the human HIF-1αgene （rAAV-HIF-1α）, and tested the assumption that rAAV-HIF-1αrepresses hippocampal neuronal apoptosis induced by amyloid-beta protein. Our results conifrmed that rAAV-HIF-1αsigniifcant-ly reduces apoptosis induced by amyloid-beta protein in primary cultured hippocampal neurons. Direct intracerebral rAAV-HIF-1αadministration also induced robust and prolonged HIF-1αproduction in rat hippocampus. Single rAAV-HIF-1αadministration resulted in decreased apoptosis of hippocampal neurons in an Alzheimer＇s disease rat model established by intrace-rebroventricular injection of aggregated amyloid-beta protein （25-35）. Our in vitro and in vivo ifndings demonstrate that HIF-1 has potential for attenuating hippocampal neuronal apoptosis induced by amyloid-beta protein, and provides experimental support for treatment of neurode-generative diseases using gene therapy.
文摘Objective:To compare effect of intrathecal meperidine,tramadol,magnesium sulfate,and dexmedetomidine on the prevention of post-spinal anesthesia shivering and adverse events in hip fracture repair patients.Methods:In a randomized,double-blind trial,132 patients with American Society of Anesthesiology(ASA)ⅠandⅡspinal anesthesia who needed hip fracture surgery were enrolled.Patients were stratified into 4 intervention groups based on a randomized block pattern:meperidine,tramadol,magnesium sulfate,and dexmedetomidine.Hemodynamic parameters including blood pressure,heart rate,and oxygen saturation,as well as the severity of shivering,core body temperature,Ramsay sedation score,adverse events,meperidine consumption were recorded and compared.Results:There was no statistically significant difference in the normal hemodynamic parameters,temperature,duration of surgery,meperidine consumption,and adverse events such as dizziness,hypotension,nausea,and bradycardia among groups(P>0.05).Compared to other groups,severity of shivering was the lower in the dexmedetomidine group 6 and 8 h after surgery.The Ramsay sedation scores were higher in the dexmedetomidine and meperidine groups 4 h after surgery(P=0.020).Conclusion:Dexmedetomidine acts better than the other three adjuvants in reducing complications such as shivering.Overall,these four adjuvants are helpful to prevent postoperative shivering and could be put forward as promising local anesthetics in spinal anesthesia,based on anesthesiologists’discretion and patients’general conditions.Clinical registration:The study was approved by the Research and Ethics Committee at the Valiasr Hospital(Arak,Iran)with the clinical trial code of IRCT20141209020258N153.
文摘OBJECTIVE: This study used gated myocardial perfusion imaging(G-MPI) to assess the clinical value of Xinmailong injection in chronic congestive heart failure(CHF).METHODS: A total of 102 CHF patients were randomly divided into the control group(n=51) and the Xinmailong group(n=51). Patients in the control group were routinely treated. Patients in the Xinmailong group were additionally treated with Xinmailong injection in addition to routine treatment. Before and 3 months after treatment, G-MPI was used to determine changes in the left ventricular ejection fraction(LVEF), left ventricular end-diastolic volume(LVEDV) and left ventricular end-systolic volume(LVESV). Fourteen days after treatment, changes in plasma brain natriuretic peptide(BNP) levels were determined.RESULTS: Before treatment, there were no significant differences in LVEF, LVEDV, LVESV, and BNP levels between the two groups(all P>0.05). After treatment, LVEDV, LVESV, and BNP levels were significantly lower, and LVEF was significantly higher in the Xinmailong group than in the control group(all P<0.05).CONCLUSION: Additional use of Xinmailong injection in addition to routine treatment improves cardiac function of CHF patients. Because of the safety and effectiveness of Xinmailong injection, this therapy should be promoted.
文摘Based on spin-flip model （SFM）, the nonlinear dynamics of 1550nm vertical-cavity surface-emitting lasers （VCSELs） subject to polarization-preserved optical feedback （PPOF） and orthogonal optical injection （OOI） are theoretically investigated. The results show that two linear polarization （LP） modes can be simultaneously stimulated and polarization switching （PS） can be observed, which is different from the case that only y LP mode exists in a VCSEL with PPOF. Under the joint action of PPOF and OOI, the two LP modes will exhibit rich dynamical states, such as period one, period two, multi- period and chaos oscillation. Different evolution routes to chaos can be also observed. Moreover, frequency detuning Af（Af=fm fs, wherefn andfs are the center frequencies of free-running master VCSEL and slave VCSEL, respectively） has an obvious influence on the PS. With the increase of the positive frequency detuning PS points shift toward larger injection strengths; meanwhile, a suitable negative frequency demning value makes the injection strength for PS be the lowest.
