Effect of stimulating the acupoints Feishu (BL 13) and Dazhui (GV14) on transdermal uptake of sinapine thiocyanate in asthma gel

OBJECTIVE: To investigate the effect of stimulating the acupoints Feishu(BL 13) and Dazhui(GV 14) on the transdermal uptake of sinapine thiocyanate contained in a gel used for the management of asthma.METHODS: Thirty Sprague-Dawley rats were randomly divided into three equal groups using a random number table: the Feishu(BL 13) acupoint group, the Dazhui(GV 14) acupoint group, and the nonacupoint group or control group. Using micro-dialysis technology, preprocessed skin probes were implanted into the rats at Feishu(BL 13), Dazhui(GV 14), and a nonacupoint site. Asthma gel was then placed on the skin at Feishu(BL 13), Dazhui(GV14) acupoints, and the nonacupoint for all groups.Dialysate was collected every 30 min for 12 h. The normalized concentration of sinapine thiocyanate in the skin was determined by high-performance liquid chromatography.RESULTS: The rat in vivo transdermal experiment demonstrated that the quantity-time equation showed a good linear correlation with zero-order kinetics(r > 0.99). The transdermal behavior was in accordance with the first-order rate open model in which the transdermal penetration rates and the accumulative amounts of sinapine thiocyanate in the skin at the acupoint sites were greater than those through the skin of the nonacupoint site. The systemic maximum concentration and the area under the curve of sinapine thiocyanate in the acupoint groups were significantly greater than in the nonacupoint group. A lag time was observed in both acupoint groups, but not in the nonacupoint group.CONCLUSION: Stimulating the acupoints promotes the percutaneous absorption of sinapine thiocyanate and also controls its release, reducing concentration fluctuations in the blood.

Effect of scar-producing moxibustion at the acupoints Zusanli(ST 36) and Feishu(BL 13) on neutrophil-to-lymphocyte ratio and quality of life in patients with non-small-cell lung cancer: A randomized,controlled trial

OBJECTIVE: To evaluate the effect of heat stimulation via scar-producing moxibustion at the acupoints Zusanli(ST 36) and Feishu(BL 13) on the neutrophil-to-lymphocyte ratio(NLR) and quality of life in patients with non-small-cell lung cancer(NSCLC).METHODS: Seventy patients with NSCLC were randomly assigned into two groups: group A received scar-producing moxibustion at the acupoints Zusanli(ST 36) and Feishu(BL 13) every day for 6 weeks, while group B received no intervention(control group). Outcome measures were the NLR and the scores from the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire(EORTC QLQ-C30). The NLR and the EORTC QLQ-C30 were assessed at baseline and at the end of 6 weeks.RESULTS: Five participants dropped out, leaving afinal total of 65 participants who completed the trial. Groups A and B had a similar mean NLR at baseline. After the treatment course, the NLR in group A was significantly lower than that in group B(P <0.001). Compared with group B, the EORTC QLQ-C30 scores in group A were significantly greater in terms of global health status or quality of life(P < 0.001) and function(P < 0.05), and significantly lower in terms of symptoms(P < 0.05).CONCLUSION: The present study suggests that performing scar-producing moxibustion by heat-stimulating the acupoints Zusanli(ST 36) and Feishu(BL13) effectively decreases the NLR and improves the quality of life in patients with NSCLC.

Clinical observation on acupoint injection of Chuankezhi injection plus acupoint sticking in treating bronchial asthma

Objective: To observe the clinical efficacy of acupoint injection of Chuankezhi plus acupoint sticking treatment for bronchial asthma, and provide clinical evidence for the synergy of the two therapies, and explore their synergistic mechanism. Methods: A total of 70 patients were randomized into an acupoint injection plus sticking group and an acupiont sticking group by the random number table, with 35 cases in each group. The treatment took place in July and August. The acupoint injection plus sticking group was treated with acupoint injection and acupiont sticking, while the acupiont sticking group was treated only with acupiont sticking therapy. The treatment course was 4 weeks. After the treatment, the scores of symptom scale in the two groups before treatment, 3 months and 6 months after the treatment were observed. Results: During the treatment, there were 5 dropouts in the acupoint injection plus sticking group with 30 cases remained, and 4 dropouts in the acupiont sticking group with 31 cases remained. Before the treatment, there was no significant difference in the total scores of symptom scale between the two groups. Three months after the treatment, the total scores of symptom scale of both groups were lower than those before treatment, and the intra-group differences were statistically significant (bothP<0.05). In the inter-group comparison, there was no significant difference in the difference values of the scores before treatment and 3 months after treatment (P>0.05). Six months after the treatment, the total scores of symptom scale in both groups were lower than those before treatment, and the intra-group differences were statistically significant (bothP<0.05), so were the differences in the difference values of the scores before treatment and 6 months after treatment (bothP<0.05). Conclusion: Acupoint injection of Chuankezhi plus acupoint sticking or acupoint sticking alone both can improve the symptoms of patients with bronchial asthma. Acupoint injection of Chuankezhi plus acupoint sticking has a higher total effective rate than single acupoint sticking therapy.

Treatment and Prevention of 100 Cases with Allergic Rhinitis by Acupoint Sticking Therapy

Objective: To observe the clinical effects of acupoint sticking therapy in the three ten-day periods in summer and three nine-day periods in winter. Methods: Herbal medicines were prepared into crude herbal powder and made into paste with ginger juice, and were respectively applied to the corresponding acupoints in the three ten-day periods in summer and three nine-day periods in winter. Results: The results showed 45 cases were cured, 50 cases were improved, and 5 cases failed out of 100 cases, with the total effective rate of 95%. Conclusion: The acupoint sticking therapy for the treatment and prevention of allergic rhinitis is simple in method, remarkable in effects, acceptable to the patients, and is worthy of clinical popularization.

Electroacupuncture at Feishu(BL13) and Zusanli(ST36) down-regulates the expression of orexins and their receptors in rats with chronic obstructive pulmonary disease

Inflammation and lung function decline are the main pathophysiological features of chronic obstructive pulmonary disease （COPD）. Acupuncture can improve lung function in patients with COPD, but the underlying mechanisms are not well understood. Orexins （OXs）, which are found in peripheral plasma, are neuropeptides that regulate respiration and their levels are related to COPD. Therefore, we hypothesized that acupuncture might alter OXs, reduce lung inflammation and improve lung function in COPD. METHODS： COPD was induced in rats by exposure to cigarette smoke for 8 weeks and injecting with lipopolysaccharide twice. Electroacupuncture （EA） was performed at Feishu （BL13） and Zusanli （ST36） for 30 min/d for 2 weeks. Rat lung function and morphology were assessed after EA. The levels of tumor necrosis factor-α （TNF-a） and intedeukin-1β （IL-16） in bronchoalveolar lavage fluid （BALF） and orexin A and B levels in the lung tissue were detected by enzyme-linked immunosorbent assay. OX receptor mRNA levels and immunopositive cells were assessed with real-time polymerase chain reaction and immunohistochemical methods, respectively. The relationships among lung function, cell factors, and OX levels were analyzed by Pearson correlation analyses. RESULTS： Compared with the control group, lung function was significantly decreased in the rats with COPD （P〈0.05）. There were obvious increases in TNF-α and IL-1β levels in BALF （P〈0.05 and P〈0.01, respectively）, orexin A level in lung tissue （P〈0.01; but not orexin B） and mRNA expressions of OX 1 type receptor （OXR1） and OX 2 type receptor （OXR2） in lung tissue （P〈0.05 and P〈0.01, respectively）; the integrative optical densities （IODs） of both receptors were greater in the COPD group （P〈0.05）. For rats with COPD subjected to EA, lung function was improved （P〈0.05）. There were notable decreases in TNF-a and IL-1β levels （P〈0.05 and 〈0.01, respectively） in BALF. Orexin A, but not orexin B, levels in lung tissue also decreased （P〈0.01）, as did mRNA expression of OXlR and OX2R in lung tissue （P〈0.05 and P〈0.01, respectively）. Receptor IODs were also reduced after EA treatment （P〈0.05）. Furthermore, orexin A levels and ratio of forced expiratory volume in 0.3 s to forced vital capacity were strongly negatively correlated （P〈0.01）, and orexin A was positively correlated with TNF-α and IL-1β （P〈0.001 and P〈0.05, respectively）.CONCLUSION： EA at Zusanli and Feishu improved lung function of rats with COPD and had an anti-inflammatory effect, which may be related to down-regulation of OXA and its receptors.

Effect of moxibustion at Feishu(BL 13)on airway inflammation in asthma model rats

Objective:To observe the effect of moxibustion at Feishu(BL 13)on related inflammatory cells and inflammatory factors in the bronchoalveolar lavage fluid of asthma model rats,and to explore the mechanism of moxibustion in treating asthma.Methods:A total of 48 Sprague-Dawley(SD)rats were randomly divided into a normal group,a model group,a moxibustion group and a medication group,with 12 rats in each group.Except for the normal group,the rats in the other three groups were subjected to ovalbumin sensitization to stimulate the asthma.At the same time,rats in the moxibustion group received moxibustion at bilateral Feishu(BL 13),and rats in the medication group received dexamethasone by intragastric administration.Rats in the normal and the model groups only received the same fixation and normal saline by intragastric administration.After the interventions,the inspiratory resistance,the expiratory resistance,and the pulmonary compliance were measured for rats in each group;the numbers of the inflammatory cells in the bronchoalveolar lavage fluid were counted;the levels of the involved inflammatory factors in bronchoalveolar lavage fluid were detected;the pathological morphologies of the lung tissues were observed under light microscope.Results:After modeling,compared with the normal group,the rats in the model group showed obvious asthma attack-like response,significantly increased inspiratory resistance and expiratory resistance(both P<0.01),and significantly reduced pulmonary compliance(P<0.01);thickened tracheal wall and the narrowed tracheal lumen observed under the light microscope;infiltration of inflammatory cells and increased eosinophils in and around the tracheal wall;significantly increased total number of inflammatory cells and proportion of eosinophils in the bronchoalveolar lavage fluid(all P<0.01);significantly reduced levels of interleukin(IL)-10,IL-12 and interferon(IFN)-γ(all P<0.01),and significantly increased levels of IL-4,IL-5 and tumor necrosis factor(TNF)-α(all P<0.01)in the bronchoalveolar lavage fluid.After intervention,compared with the model group,rats in the moxibustion and the medication groups showed significantly reduced asthma-like reaction,pathological morphological damage of lung tissue,inspiratory resistance and expiratory resistance(all P<0.01);significantly increased pulmonary compliance(P<0.01);significantly reduced total number of inflammatory cells,proportion of eosinophils,levels of IL-4,IL-5 and TNF-αin the bronchoalveolar lavage fluid(P<0.05 or P<0.01),while significantly increased IL-12 and IFN-γlevels(all P<0.01)in the bronchoalveolar lavage fluid;rats in the medication group also showed a significantly reduced IL-10 level in the bronchoalveolar lavage fluid(P<0.01);there was no statistically significant difference between the moxibustion and the medication groups(all P>0.05).Conclusion:Both moxibustion at Feishu(BL 13)and intragastric administration of dexamethasone can improve the asthma attack-like symptoms of ovalbumin-sensitized rats;regulating the inflammatory cell numbers and the inflammatory factor contents in the lung may be one mechanism of moxibustion in treating asthma.

Effects of moxibustion at bilateral Feishu(BL13)and Xinshu(BL15)combined with benazepril on myocardial cells apoptosis index and apoptosis-related proteins cytochrome c and apoptosis-inducing factor in rats with chronic heart failure

OBJECTIVE:To observe the effects of moxibustion at bilateral Feishu(BL13)and Xinshu(BL15)combined with benazepril on myocardial cells apoptosis index,the expression levels of apoptosis-related proteins cytochrome c(Cyt-C)and apoptosis-inducing factor(AIF)in chronic heart failure(CHF)rats.METHODS:Sixty-five rats were randomly divided into normal group(n=10)and model-I group(n=55).After modeling,CHF rats in model-I group were divided into model group,moxibustion group,benazepril group,moxibustion plus benazepril group(abbreviated as aibei group,the same below),10 rats in each group.Echocardiogram index was examined by echocardiography.Hemodynamic indices were measured by rat cardiac function meter.Serum B-type brain natriuretic peptide(BNP)was detected by enzymelinked immunosorbent assay.Myocardial cells apoptosis index was detected by terminal-deoxynucleoitidyl transferase mediated nick end labeling staining.Pathological changes of myocardial tissues were observed by hematoxylin and eosin staining.The expression levels of Cyt-C and AIF in myocardial tissues were detected by Western blot.RESULTS:Compared with normal group,ejection fraction and left ventricular diameter shortening rate in model-Ⅰgroup were significantly reduced,myocardial cells of rats in model group exhibited unclear transverse striations,cells swellings and vacuoles,cardiac functions were deteriorated,serum BNP level,myocardial cells apoptosis index,and the expression levels of Cyt-C and AIF were significantly increased.Compared with model group,myocardial cells of rats in moxibustion group,benazepril group,and aibei group were dyed more evenly,muscle fibers were arranged relatively neatly,cardiac functions were improved,serum BNP level,myocardial cells apoptosis index,and the expression levels of Cyt-C and AIF were significantly decreased.Compared with aibei group,cardiac functions were worsened,myocardial cells apoptosis index,and the expression levels of Cyt-C and AIF were increased.CONCLUSION:Moxibustion at bilateral Feishu(BL13)and Xinshu(BL15)combined with benazepril could improve CHF better than moxibustion at bilateral Feishu(BL13)and Xinshu(BL15)or benazepril alone.The mechanisms might be that they can inhibit the expressions of Cyt-C and AIF,and inhibit the apoptosis of cardiomyocytes.

针刺肺俞穴及非经非穴点对肺功能影响的对照研究

目的 :探讨针刺肺俞穴及非经非穴点对肺功能的影响。方法 :选健康男性学员 80名 ,年龄2 0～ 2 2岁之间 ,随机分为两组 ,应用美制 2 12 0肺功能仪分别于针刺前 10min及针刺后 10min测试FVC、FEV1 0 、FEV1 0 %、MMF、PEFR、·V75、·V50 、·V2 5。结果 :针刺肺俞穴后FVC明显增高 (P <0 0 5 ) ,针刺非经非穴点后 ,各项指标虽有不同程度的提高 ,但无统计学意义 (P >0 0 5 )。 结论 :针刺肺俞穴可提高健康人的FVC ,而针刺非经非穴点则无此作用。

针刺肺俞及四花穴对慢性支气管炎迁延期老年患者免疫球蛋白的影响

目的观察针刺肺俞及四花(膈俞和胆俞)穴对慢性支气管炎迁延期老年患者免疫球蛋白的影响。方法将60例老年慢性支气管炎迁延期患者随机分为治疗组和对照组,每组30例。两组均采用常规药物治疗,治疗组采用针刺肺俞及四花穴治疗,对照组采用口服蜜炼川贝枇杷膏治疗。分别观察两组治疗前后免疫球蛋白变化。结果治疗组治疗后免疫球蛋白各项指标(IgA、IgM、IgG)与同组治疗前比较,差异均具有统计学意义(P<0.05)。治疗组治疗后免疫球蛋白各项指标与对照组比较,差异均具有统计学意义(P<0.05)。结论针刺肺俞及四花穴对慢性支气管炎迁延期老年患者免疫球蛋白具有升高作用。

大椎穴拔罐前后对健康成人左肺俞及其旁开非经穴的氧分压变化的影响

目的通过观察拔罐疗法对穴位局部组织氧分压改变的影响,探讨其对能量代谢影响的机理。方法该试验运用自制复合式氧分压传感器及智能型四通道氧分压测试仪进行动态连续实时监测,观察健康人体大椎穴拔罐前后对左肺俞及其旁开非经穴的氧分压变化的影响。结果左肺俞的氧分压从起罐5 min开始至20 min各时间点与基础5 min、拔罐5 min比较均具有统计学意义(P<0.05),旁开非经穴的氧分压在各时间点均无统计学差异(P>0.05);在基础5 min与拔罐5 min时,左肺俞的氧分压与旁开非经穴比较均有统计学差异(P<0.05)。结论拔罐大椎穴5 min后,左肺俞处组织随时间变化氧分压下降,起罐20 min时略有回升。大椎穴拔罐可能致使左肺俞处组织耗氧增多,能量代谢加快。旁开非经穴处组织,氧分压变化随时间变化无明显差异;生理状态下,左肺俞的含氧量可能高于旁开非经穴。拔罐后左肺俞处能量代谢更明显。

艾灸肺俞、心俞治疗慢性心衰临床观察

目的观察艾灸配合西药治疗慢性心衰的临床疗效。方法将60例患者随机分为对照组和治疗组,每组30例。对照组给予西药常规治疗;治疗组在西药常规治疗的基础上,采用艾条温和灸双侧肺俞、心俞穴,每穴灸治20 min,每日1次,共灸治4星期。比较两组治疗后临床疗效和心功能相关指标。结果对照组总有效率为76.7%,治疗组总有效率为90.0%,治疗组优于对照组(P<0.05);两组治疗后HR、CO、LVEF较治疗前有显著改善(P<0.01),治疗组治疗后HR、CO、LVEF与对照组比较有显著差异(P<0.01),治疗组治疗后HR、CO、LVEF改善状况优于对照组。结论艾灸配合西药治疗慢性心衰临床疗效优于单纯西药治疗。

针刺肺俞穴改善肺功能时效关系研究

通过针刺肺俞穴，观察不同留针时间对呼吸系统疾病患者肺功能改善情况。结果表明，针刺肺俞穴能明显改善肺功能，在留针２０分钟时可见针刺效应，４０分钟时针刺效应最明显，６０分钟时针刺效应有所降低。提示不同留针时间对肺功能的改善效应是不同的。

穴位注射治疗过敏性鼻炎40例

穴位注射治疗过敏性鼻炎４０例杨玉玲，罗红强（江西省人民医院，南昌３３０００６）笔者近４年来用地塞米松注射迎香穴治疗过敏性鼻炎４０例，收到较好的疗效，现报告如下。１临床资料一般资料：４０例患者中，男２９例，女１１例；年龄最小１５岁，最大６１岁；病程小于...

穴位贴敷治疗过敏性鼻炎选穴及用药规律

目的分析现代文献中穴位贴敷治疗过敏性鼻炎的选穴及用药规律,为临床治疗该病提供依据。方法采用计算机检索的方式,检索中国期刊全文数据库(CNKI)、万方数据知识服务平台(WF)及维普期刊数据库(VIP)自建刊以来符合穴位贴敷治疗过敏性鼻炎的相关文献,对其进行整理、筛选、统计及分析。结果最终纳入有效文献123篇,其中包含37个穴位、43味中药。选穴以腰背部的足太阳膀胱经穴为主,穴位类型以背俞穴为主,肺俞穴居首位;用药多选取发泡药物,以白芥子、细辛、甘遂、延胡索为多,多归于肺、脾经,药性多温,药味以辛、苦、甘味为主。结论中药穴位贴敷治疗过敏性鼻炎在改善症状方面有着一定的优势,其选穴及用药的规律性可为今后的临床治疗提供参考和借鉴。

风灸疗法临床体会

风灸疗法临床体会徐敬堂，唐永芬（安徽中医学院中医系，合肥２３００３８）风灸疗法是传统中医灸疗与现代科技电热效应相结合的一种灸疗法。它的治疗作用机理是通过电产生的热力，将装在风灸仪内中药配方的性味功能充分挥发出来，以热药风的形式，直接吹到人体皮肤、经络...

中药肺俞穴敷贴为主治疗黑人小儿支气管肺炎163例

323例埃塞俄比亚黑人小儿支气管肺炎随机分为2组。治疗组163例,采用自制中药肺俞穴敷贴结合常规西药治疗;对照组160例,采用安慰剂肺俞穴敷贴结合常规西药治疗。结果:治疗组的肺部湿性啰音消失率、X线情况改善率、平均治愈时间等均优于对照组。

毫针散刺治疗慢性筋膜炎216例

毫针散刺治疗慢性筋膜炎２１６例张倩如，程建东（广东省珠海市中医院针灸科，５１９０１５）主题词毫针，筋膜炎／针灸疗法筋膜位于肌肉的表面，分浅、深筋膜两种，分别由疏松结缔组织和致密结缔组织构成，是肌肉的一种保护装置，参与肌肉起止点的形成，亦能协助肌肉间产...

肺俞穴在小儿哮喘治疗中的应用

哮喘的病因病机主要责之于脾、肺、肾三脏功能失调,致使留痰伏饮,遇外感而发。其发作期的治疗宜宣肺化痰平喘,缓解期的治疗宜调理肺、脾、肾三脏功能,化痰逐饮。肺俞、定喘、大椎是治疗小儿哮喘的常用穴位。肺俞穴不仅是邪气入侵肺脏的部位,也是反映病证,接受刺激,达到治疗作用的特殊穴位。肺俞穴能改善肺功能,具有调补肺气,补虚清热的功效,主治呼吸系统疾病及与气有关的疾病。肺俞穴在中医外治治疗小儿哮喘方面虽然积累了较丰富的经验但是仍然存在一些问题:外治法大部分治疗是建立在西医或中药治疗基础上的辅助治疗;虽然中医外治治疗哮喘的取穴以肺俞穴为主,但目前与其配穴的选择暂不统一。治疗小儿哮喘的科学性、规范性,以及穴位组方规律,直接关系到临床疗效的可靠性及稳定性问题,仍需进一步进行研究。

穴位注射治疗眼部带状疱疹临床研究

目的:观察穴位注射治疗眼部带状疱疹的临床疗效。方法:将80例眼部带状疱疹患者随机分为对照组和治疗组,每组各40例。对照组给予常规治疗,治疗组在对照组的基础上加用穴位注射治疗。观察两组患者的临床疗效及各项指标恢复时间。结果:对照组有效率为75.00%,治疗组有效率为90.00%,两组患者临床疗效比较,差异有统计学意义(P<0.05)。治疗组止痛天数、疱疹结痂时间及眼部损伤好转天数明显短于对照组,差异有统计学意义(P<0.05)。结论:穴位注射治疗眼部带状疱疹临床疗效显著,且能够缩短病程,减轻患者疼痛程度。

灸肺俞刺四关治疗阴暑症临床疗效观察

目的观察灸肺俞刺四关治疗阴暑症的临床疗效。方法将100例阴暑症患者随机分为治疗组和对照组,各50例;治疗组采用灸肺俞刺四关治疗,对照组采用常规针灸治疗,疗程3天。结果治疗组和对照组的治愈率分别为100%、52%,两者比较有显著性差异(P<0.05)。结论灸肺俞刺四关治疗阴暑症的临床疗效确切。