We used the flow cytometric immunoassay to study the correlation between the tumor-suppressor gene product p53- and the DNA ploidy in 30 de novo cases of acute nonlymphocytic leukemia (ANLL).The results showed that 15...We used the flow cytometric immunoassay to study the correlation between the tumor-suppressor gene product p53- and the DNA ploidy in 30 de novo cases of acute nonlymphocytic leukemia (ANLL).The results showed that 15 cases were negative and the other 15 cases were positive expression for p53. As compared with p53 negative (p53) cases, the patients with positive p53 (p53+) had higher percentage of bone marrow blasts and lower peripheral leukocyte and platelet counts,which had no influence on the complete remission rate. Before treatment, DNA diploidy was seen in 18 cases including 12 p53- cases, and DNA aneuploidy in 12 cases including 9 p53+. After therapy, aneuploidy could be transformed into diploidy.Patients with P53+ or having aneuploidy in complete remission were at risk for early relapse. We believe that p53 may be involved in the process of leukemogenesis and progression of ANLL.展开更多
Chromosomal studies were performed in the same laboratory on 97 untreated cases of de novo acute nonlymphocytic leukemia M2.The overall incidence of chromosomal abnormality was 70.1% (68 out of 97 cases),which was hig...Chromosomal studies were performed in the same laboratory on 97 untreated cases of de novo acute nonlymphocytic leukemia M2.The overall incidence of chromosomal abnormality was 70.1% (68 out of 97 cases),which was higher in children(84. 2% )than in edults (61%).The male to female chromosomal abnormality ratio was nearly the same(male 71% and female 68. 4% .P>0.05).Hypodiploidy was the most common numerical abnormality(39%)and t (8;21) was the most common structural abnormality (48.1%) It the patients with t (8;21),64 5%(2) out of 31cases) male lost chromosome Y (-Y) and 33%(5 out of 15 cases)female lost one chromosomeX(-X).展开更多
Bone marrow studies including morphological,morphometrical and ultrastructural aspects were performed in 35 patients with M2/t(8;21)and and 23 patients with M2/NN.It was found that M2/t(8;21)patients had higher cellul...Bone marrow studies including morphological,morphometrical and ultrastructural aspects were performed in 35 patients with M2/t(8;21)and and 23 patients with M2/NN.It was found that M2/t(8;21)patients had higher cellularity in bone marrow.Type(Ⅱ)myeloblast cells were predominant among myeoloblasts.Deformation of nuclei,nucleocytoplasmic asynchronism and dysmegakaryctytopoiesis were more evident n M2/t (8;21) than in M2/NN patients.展开更多
文摘We used the flow cytometric immunoassay to study the correlation between the tumor-suppressor gene product p53- and the DNA ploidy in 30 de novo cases of acute nonlymphocytic leukemia (ANLL).The results showed that 15 cases were negative and the other 15 cases were positive expression for p53. As compared with p53 negative (p53) cases, the patients with positive p53 (p53+) had higher percentage of bone marrow blasts and lower peripheral leukocyte and platelet counts,which had no influence on the complete remission rate. Before treatment, DNA diploidy was seen in 18 cases including 12 p53- cases, and DNA aneuploidy in 12 cases including 9 p53+. After therapy, aneuploidy could be transformed into diploidy.Patients with P53+ or having aneuploidy in complete remission were at risk for early relapse. We believe that p53 may be involved in the process of leukemogenesis and progression of ANLL.
文摘Chromosomal studies were performed in the same laboratory on 97 untreated cases of de novo acute nonlymphocytic leukemia M2.The overall incidence of chromosomal abnormality was 70.1% (68 out of 97 cases),which was higher in children(84. 2% )than in edults (61%).The male to female chromosomal abnormality ratio was nearly the same(male 71% and female 68. 4% .P>0.05).Hypodiploidy was the most common numerical abnormality(39%)and t (8;21) was the most common structural abnormality (48.1%) It the patients with t (8;21),64 5%(2) out of 31cases) male lost chromosome Y (-Y) and 33%(5 out of 15 cases)female lost one chromosomeX(-X).
文摘Bone marrow studies including morphological,morphometrical and ultrastructural aspects were performed in 35 patients with M2/t(8;21)and and 23 patients with M2/NN.It was found that M2/t(8;21)patients had higher cellularity in bone marrow.Type(Ⅱ)myeloblast cells were predominant among myeoloblasts.Deformation of nuclei,nucleocytoplasmic asynchronism and dysmegakaryctytopoiesis were more evident n M2/t (8;21) than in M2/NN patients.
