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Plasma levels of acylated ghrelin in patients with functional dyspepsia 被引量:12
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作者 Yeon Soo Kim Joon Seong Lee +6 位作者 Tae Hee Lee Joo Young Cho Jin Oh Kim Wan Jung Kim Hyun Gun Kim Seong Ran Jeon Hoe Su Jeong 《World Journal of Gastroenterology》 SCIE CAS CSCD 2012年第18期2231-2237,共7页
AIM:To investigate the relationship between plasma acylated ghrelin levels and the pathophysiology of functional dyspepsia.METHODS:Twenty-two female patients with functional dyspepsia and twelve healthy volunteers wer... AIM:To investigate the relationship between plasma acylated ghrelin levels and the pathophysiology of functional dyspepsia.METHODS:Twenty-two female patients with functional dyspepsia and twelve healthy volunteers were recruited for the study.The functional dyspepsia patients were each diagnosed based on the Rome Ⅲ criteria.Eligible patients completed a questionnaire concerning the severity of 10 symptoms.Plasma acylated ghrelin levels before and after a meal were determined in the study participants using a commercial human acylated enzyme immunoassay kit;electrogastrograms were performed for 50 min before and after a standardized 10-min meal containing 265 kcal.RESULTS:There were no significant differences in plasma acylated ghrelin levels between healthy volunteers and patients with functional dyspepsia.However,in patients with functional dyspepsia,there was a negative correlation between fasting plasma acylated ghrelin levels and the sum score of epigastric pain(r =-0.427,P = 0.047) and a positive correlation between the postprandial/fasting plasma acylated ghrelin ratio and the sum score of early satiety(r = 0.428,P =0.047).Additionally,there was a negative correlation between fasting acylated ghrelin plasma levels and fasting normogastria(%)(r =-0.522,P = 0.013).Interestingly,two functional dyspepsia patients showed paradoxically elevated plasma acylated ghrelin levels after the meal.CONCLUSION:Abnormal plasma acylated ghrelin levels before or after a meal may be related to several of the dyspeptic symptoms seen in patients with functional dyspepsia. 展开更多
关键词 Functional dyspepsia acylated ghrelin Electrogastrogram Rome criteria Dyspeptic symptoms
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Diacylated Triterpenoid Saponin from Silene szechuensis 被引量:2
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作者 Cheng ZOU Jia Xiong JIANG +2 位作者 Chang Xiang CHEN Jun ZHOU Qing ZHAO(Faculty of Pharmacy, Kunming Medical College, Kunming 650031)(Kunming Institute of Botany, the Chinese Academy of Sciences, Kunming 650204)(Yunnan College of Traditional Chinese Medicine. Ku 《Chinese Chemical Letters》 SCIE CAS CSCD 1999年第1期33-36,共4页
A new triterpenoid saponin, named silenoside, with sugar chains esterified by two different acyl groups was isolated from Silene szechuensis. Its structure was elucidated by chemical and spectral methods.
关键词 silenoside acylated triterpenoids saponin Silene szechuensis
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Inhibition of DNA-Topoisomerase I by Acylated Triterpene Saponins from Pittosporum angustifolium Lodd. 被引量:2
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作者 Christian Backer Malgorzata N.Drwal +1 位作者 Robert Preissner Ulrike Lindequist 《Natural Products and Bioprospecting》 CAS 2016年第2期141-147,共7页
Previous phytochemical investigation of the leaves and seeds of Pittosporum angustifolium Lodd.led to the isolation and structural elucidation of polyphenols and triterpene saponins.Evaluation for cytotoxicity of isol... Previous phytochemical investigation of the leaves and seeds of Pittosporum angustifolium Lodd.led to the isolation and structural elucidation of polyphenols and triterpene saponins.Evaluation for cytotoxicity of isolated saponins revealed that the predominant structural feature for a cytotoxic activity are acyl substituents at the oleanane aglycon backbone.The present work reports the results of a screening of 10 selected acylated saponins for their potential to inhibit the human DNA-topoisomerase I,giving rise to IC50 values in a range of 2.8-46.5 lM.To clarify the mode of observed cytotoxic action and,moreover,to distinguish from a pure surfactant effect which is commonly accompanied with saponins,these results indicate an involvement of the topoisomerase I and its role as a possible target structure for a cytotoxic activity.In addition,computational predictions of the fitting of saponins to the topoisomerase I-DNA complex,indicate a similar binding mode to that of clinically used topoisomerase I inhibitors.Graphical Abstract Ten acylated triterpene saponins from Pittosporum angustifolium were investigated for their potential to inhibit the human DNA-topoisomerase I and computational predictions of the fitting of saponins to the topoisomerase I-DNA complex were carried out. 展开更多
关键词 Pittosporum angustifolium acylated triterpene saponins CYTOTOXICITY Topoisomerase I DOCKING
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Synthesis and properties of nickel borate acylate as a new rubber-steel cord adhesion promoter 被引量:1
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作者 LI Xiao ru 1,NIE Xi du 2,WU Hai ying 1,CHENG Ben cheng 1 (1 College of Chemistry and Chemical Engineering, Central South University, Changsha 410083, China 2 Hengyang Branch of Hunan University, Hengyang 412000, China) 《Journal of Central South University of Technology》 2001年第4期244-248,共5页
The synthetic method of nickel borate acylate (NBA), a kind of rubber steel cord adhesion promoter (AP), through nickel carbonate, borate and mixed carboxylic acid was studied. The preparation of nickel carbonate coul... The synthetic method of nickel borate acylate (NBA), a kind of rubber steel cord adhesion promoter (AP), through nickel carbonate, borate and mixed carboxylic acid was studied. The preparation of nickel carbonate could be performed through reaction of nickel sulfate with sodium carbonate in aqueous solution, in which fractional conversion of nickel was more than 99.9%. The mixed nickel carboxylate was prepared by the reaction of nickel carbonate with isooctanoic acid and acetic acid, under strong stirring for 2 h, the mole ratio of these chemicals were 1∶1∶1.1 respectively, and water should be removed completely by adding of inert organic solvent after reaction was finished so as to avoid hydrolysis of tributyl borate in the second reaction. NBA was synthesized by reaction of mixed nickel carboxylate with tributyl borate under strong stirring at 200 250 ℃ for 6 h. By detection and comparison with 680C product from Manobond Company of England, the NBA synthesized through isooctanoic acid and tributyl borate was very similar to product 680C in IR data, 300% fixed extension strength, tensile strength, hardness and cure curve, and was slightly lower than those of product 680C in tensile failure extensibility and hot air aging. The experimental results show that the prepared NBA can be used as rubber steel cord AP. 展开更多
关键词 NICKEL BORATE acylate ADHESION PROMOTER NICKEL CARBONATE BORATE
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Preparation and Characterization of Acylated Chitosan 被引量:2
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作者 LIMing-ehun LIUChao XINMei-hua ZHAOHuang WANGMin FENGZhen SUNXiao-li 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2005年第1期114-116,共3页
Fully acylated chitosan and N, N-diacyl chitosan were prepared. The products were characterized by elemental analysis, FTIR and 1H NMR. The experimental results indicate that the average degree of acylation depends ... Fully acylated chitosan and N, N-diacyl chitosan were prepared. The products were characterized by elemental analysis, FTIR and 1H NMR. The experimental results indicate that the average degree of acylation depends on the volume ratio of pyridine to chloroform in the reaction medium, the chain length of the acylation agent used, and the molecular weight of chitosan raw materials. The XRD measurements were carried out for pure chitosan, fully acylated chitosan and N, N-diacyl chitosan to verify the crystallinity change caused by the acylation. 展开更多
关键词 CHITOSAN ACYLATION Degree of substitution XRD
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A New Acylated Anthocyanin from the Red Flowers of Camellia hongkongensis and Characterization of Anthocyanins in the Section Camellia Species 被引量:15
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作者 Fumio Hashimoto Keiichi Shimizu Yusuke Sakata 《Journal of Integrative Plant Biology》 SCIE CAS CSCD 2009年第6期545-552,共8页
Twelve anthocyanins (1-12) were isolated from the red flowers of Camellia hongkongensis Seem. by chromatography using open columns. Their structures were elucidated on the basis of spectroscopic analyses, that is, p... Twelve anthocyanins (1-12) were isolated from the red flowers of Camellia hongkongensis Seem. by chromatography using open columns. Their structures were elucidated on the basis of spectroscopic analyses, that is, proton-nuclear magnetic resonance, carbon 13-nuclear magnetic resonance, heteronuclear multiple quantum correlation, heteronuclear multiple bond correlation, high resolution electrospray ionization mass and ultraviolet visible spectroscopies. Out of these anthocyanins, a novel acylated anthocyanin, cyanidin 3-O-(6-O-(Z)-p-coumaroyl)-β-galactopyranoside (6), two known acylated anthocyanins, cyanidin 3-O-(6-O-(E)-p-coumaroyl)-β-galactopyranoside (7) and cyanidin 3-O-(6-O-(E)-caffeoyl)-^- galactopyranoside (8), and three known delphinidin glycosides (10-12) were for the first time isolated from the genus Camellia. Furthermore, pigment components in C. japonica L., C. chekiangoleosa Hu and C. semiserrata Chi were studied. The results indicated that the distribution of anthocyanins was differed among these species. Delphinidin glycoside was only detected in the flowers of C. hongkongensis, which is a special and important species in the section Camellia. Based on the characterization of anthocyanins in the section Camellia species, there is a close relationship among these species, and C. hongkongensis might be an important parent for creating new cultivars with bluish flower color. 展开更多
关键词 acylated anthocyanin Camellia hongkongensis Camellia japonica Camellia chekiangoleosa Camellia semiserrata.
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The enzymatic biosynthesis of acylated steroidal glycosides and their cytotoxic activity 被引量:6
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作者 Ming Liu Jian-Qiang Kong 《Acta Pharmaceutica Sinica B》 SCIE CAS CSCD 2018年第6期981-994,共14页
Herein we describe the discovery and functional characterization of a steroidal glycosyltransferase(SGT) from Ornithogalum saundersiae and a steroidal glycoside acyltransferase(SGA) from Escherichia coli and their app... Herein we describe the discovery and functional characterization of a steroidal glycosyltransferase(SGT) from Ornithogalum saundersiae and a steroidal glycoside acyltransferase(SGA) from Escherichia coli and their application in the biosynthesis of acylated steroidal glycosides(ASGs). Initially,an SGT gene, designated as OsSGT1, was isolated from O. saundersiae. OsSGT1-containing cell free extract was then used as the biocatalyst to react with 49 structurally diverse drug-like compounds. The recombinant OsSGT1 was shown to be active against both 3β-and 17β-hydroxyl steroids. Unexpectedly,in an effort to identify OsSGT1, we found the bacteria lacA gene in lac operon actually encoded an SGA,specifically catalyzing the acetylations of sugar moieties of steroid 17β-glucosides. Finally, a novel enzymatic two-step synthesis of two ASGs, acetylated testosterone-17-O-β-glucosides(AT-17β-Gs) and acetylated estradiol-17-O-β-glucosides(AE-17β-Gs), from the abundantly available free steroids using OsSGT1 and EcSGA1 as the biocatalysts was developed. The two-step process is characterized by EcSGA1-catalyzed regioselective acylations of all hydroxyl groups on the sugar unit of unprotected steroidal glycosides(SGs) in the late stage, thereby significantly streamlining the synthetic route towards ASGs and thus forming four monoacylates. The improved cytotoxic activities of 30-acetylated testosterone17-O-β-glucoside towards seven human tumor cell lines were thus observable. 展开更多
关键词 Steroidal GLYCOSYLTRANSFERASE Steroidal GLYCOSIDE ACYLTRANSFERASE ORNITHOGALUM saundersiae acylated steroidal GLYCOSIDES LacA
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Low expression of fatty acid oxidation related gene ACADM indicates poor prognosis of renal clear cell carcinoma and is related to tumor immune infltration
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作者 JIECHUAN QIU TIANMIN YANG +3 位作者 YANNING SUN KAI SUN YINGKUN XU QINGHUA XIA 《Oncology Research》 SCIE 2024年第3期545-561,共17页
This research aims to identify the key fatty acid beta-oxidation(FAO)genes that are altered in kidney renal clear cell carcinoma(KIRC)and to analyze the role of these genes in KIRC The Gene Expression Omnibus(GEO)and ... This research aims to identify the key fatty acid beta-oxidation(FAO)genes that are altered in kidney renal clear cell carcinoma(KIRC)and to analyze the role of these genes in KIRC The Gene Expression Omnibus(GEO)and FAO datasets were used to identify these key genes.Wilcoxon rank sum test was used to assess the levels of acyl-CoA dehydrogenase medium chain(ACADM)between KIRC and non cancer samples.The logistic regression and Wilcoxon rank sum test were used to explore the association between ACADM and clinical features.The diagnostic performance of ACADM for KIRC was asessed using a diagnostic receiver operating ch aracteristic(ROC)curve.The co-expressed genes of ACADM were identifed in LinkedOmics database,and their function and pathway enrichment were analyzed.The correlation between ACADM expression level and immune infitration was analyzed by Gene Set Variation Analysis(GSVA)method Additionally,the proliferation,migration,and invasion abilities of KIRC cells were assessed after overexpressing ACADM.Following differential analysis and intersection,we identifed six hub genes,induding ACADM.We found that the expression level of ACADM was decreased in KIRC tissues and had a better diagnostic efect(AUC=0.916).Survival analysis suggested that patients with decreased ACADM expression had a worse prognosis.According to correlation analysis,a variety of dinical features were associated with the expression level of ACADML By analyzing the infiltration level of immune cells,we found that ACADM may be related to the enrichment of immune cells.Finally,ACADM overexpression inhibited proliferation,migration,and invasion of KIRC cells.In conclusion,our findings suggest that reduced ACADM expression in KIRC patients is indicative of poor prognosis.These results imply that ACADM may be a diagnostic and prognostic marker for individuals with KIRC,offering a reference for dinicians in diagnosis and treatment. 展开更多
关键词 Kidney renal clear cell carcinoma Acyl CoA dehydrogenase medium chain Immune infiltration Fatty acid oxidation Prognosis
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Synthesis of Long Chain Fatty Acids Acylated Coumarin Glycoside Esters with Lipase as Catalyst 被引量:1
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作者 LIU Duo CAI Shuanglian LUAN Fangjian WANG Qiu'an 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2015年第4期534-538,共5页
A series of novel coumarin glycoside esters(1-9) was synthesized through the acylation reaction of 4-methylcoumarin-7-O-β-D-glucoside(11) with different long chain fatty acids catalyzed by lipase in organic mediu... A series of novel coumarin glycoside esters(1-9) was synthesized through the acylation reaction of 4-methylcoumarin-7-O-β-D-glucoside(11) with different long chain fatty acids catalyzed by lipase in organic medium. The acylation occurred regioselectively at the 6'-OH of glycosyl moiety. The enzymatic synthesis was optimized to achieve 54%-70% yield using immobilized lipase(Novozym 435, 10 mg/mL) as catalyst and acetone and pyri- dine(9:1, volume ratio, water content〈1%) as solvent with an acyl donor/coumarin glycoside molar ratio of 10:1 at a temperature of 40--50 ℃ for 96 h. All the synthesized compounds were confirmed. 展开更多
关键词 Coumarin glycoside Lipase-catalyzed acylation Long chain fatty acid Regioselective acylation
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Regioselective Acylation of 2'- or 3'-Hydroxyl Group in Salicin: Hemisynthesis of Acylated Salicins
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作者 SHAO Chen PEI Yuxin +2 位作者 BORG-KARLSON Anna-Karin PEI Zhichao 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2014年第5期774-777,共4页
Salicin-based phenolic glycosides(PGs) are important defensive substances against herbivore feeding and have good bioactivities. In this work, a novel approach for the synthesis of salicin-based PGs has been develop... Salicin-based phenolic glycosides(PGs) are important defensive substances against herbivore feeding and have good bioactivities. In this work, a novel approach for the synthesis of salicin-based PGs has been developed, by which PGs of 2'-O-acetylsaliein(5a), 3'-O-acetylsalicin(gb) and 3'-O-benzoylsalicin(Sd) were hemisynthesized. The effects of acylation reagent, solvent and temperature on the regioselective acylation of 2'- or 3'-hydroxyl groups of salicin mediated by dibutyltin oxide were investigated. The optimal conditions under which the best regioselectivity reached for 5a-5d were discovered, respectively. Moreover, a tentative tin-oxygen coordination mechanism was put forward to explain the different regioselectivities shown under different conditions. 展开更多
关键词 Regioselective acylation SALICIN Phenolic glycoside Tin-oxygen coordination Hemisynthesis
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interrelationships between ghrelin, insulin and glucose homeostasis: physiological relevance 被引量:10
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作者 FranCois Chabot Alexandre Caron +1 位作者 Mathieu Laplante David H St-Pierre 《World Journal of Diabetes》 SCIE CAS 2014年第3期328-341,共14页
Ghrelin is a 28 amino acid peptide mainly derived from the oxyntic gland of the stomach. Both acylated(AG) and unacylated(UAG) forms of ghrelin are found in the circulation. Initially, AG was considered as the only bi... Ghrelin is a 28 amino acid peptide mainly derived from the oxyntic gland of the stomach. Both acylated(AG) and unacylated(UAG) forms of ghrelin are found in the circulation. Initially, AG was considered as the only bioactive form of ghrelin. However, recent advances indicate that both AG and UAG exert distinct and common effects in organisms. Soon after its discovery, ghrelin was shown to promote appetite and adiposity in animal and human models. In response to these anabolic effects, an impressive number of elements have suggested the influence of ghrelin on the regulation of metabolic functions and the development of obesityrelated disorders. However, due to the complexity ofits biochemical nature and the physiological processes it governs, some of the effects of ghrelin are still debated in the literature. Evidence suggests that ghrelin influences glucose homeostasis through the modulation of insulin secretion and insulin receptor signaling. On the other hand, insulin was also shown to influence circulating levels of ghrelin. Here, we review the relationship between ghrelin and insulin and we describe the impact of this interaction on the modulation of glucose homeostasis. 展开更多
关键词 acylated ghrelin Unacylated ghrelin Insulin secretion β-cell functions Insulin receptor signalling Glucose homeostasis
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Synthesis of Phenylpropanoid Glycoside Analogs 被引量:1
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作者 李树春 周静 李中军 《Journal of Chinese Pharmaceutical Sciences》 CAS 2004年第1期14-18,共5页
The regioselective acylation of unprotected phenylethyl glucoside withcinnamoyl chloride leads to 6-OH cin-namoylated glucoside. In this manner, thirteen phenylpropanoidglycoside analogs were designed and prepared. Th... The regioselective acylation of unprotected phenylethyl glucoside withcinnamoyl chloride leads to 6-OH cin-namoylated glucoside. In this manner, thirteen phenylpropanoidglycoside analogs were designed and prepared. Their structure was confirmed by ~1H NMR and ^(13)CNMR spectra. 展开更多
关键词 medicinal chemistry regioselective acylation phenylpropanoid glycoside glycoside analogs chemical synthesis
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Hrd1转基因小鼠的构建与验证 被引量:1
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作者 杨硕 刘磊 +5 位作者 万幸 王文丰 王娟 贺恒 雷蕾 李斌 《中华实验眼科杂志》 CAS CSCD 北大核心 2015年第2期115-117,共3页
背景 内质网应激在糖尿病视网膜病变(DR)中发挥着重要作用.羟甲基戊二酰辅酶A还原酶降解蛋白1(Hrd1,又名Syvn1)作为一种内质网相关蛋白降解(ERAD)途径中的核心蛋白,能够减少内质网应激. 目的 构建Hrd1高表达小鼠,为今后研究Hrd1... 背景 内质网应激在糖尿病视网膜病变(DR)中发挥着重要作用.羟甲基戊二酰辅酶A还原酶降解蛋白1(Hrd1,又名Syvn1)作为一种内质网相关蛋白降解(ERAD)途径中的核心蛋白,能够减少内质网应激. 目的 构建Hrd1高表达小鼠,为今后研究Hrd1在DR中的作用提供动物模型. 方法 构建Hrd1系统表达重组质粒,酶切、测序后显微注射3 p1到685枚C57BL/6小鼠受精卵中,移植到代孕母鼠,得到Hrd1转基因小鼠.利用鼠尾PCR检测法鉴定F0代小鼠的基因型.得到Hrd1基因检测阳性鼠后,使其分别与野生型鼠杂交,得到F1代Hrd1转基因小鼠,取鼠尾组织,用PCR检测法再次鉴定F1代小鼠基因型. 结果 成功构建了Hrd1重组载体,重组质粒pRP.ExBi-EF1 a-Syvn1-IRES-eGFP的全序列测定结果显示,cDNA序列均正确.接受了Hrd1-pcDNA显微注射的685枚受精卵中成活598枚.将存活的受精卵移植到代孕母鼠后共产子鼠41只,获F0代子鼠8只,生理活动均良好.PCR电泳显示339 bp处阳性条带,该基因型可以传递到子代F1.结论 成功构建了Hrd1转基因小鼠. 展开更多
关键词 羟甲基戊二酰辅酶A还原酶降解蛋白1 小鼠/转基因 内质网应激 糖尿病视网膜病变 HYDROXYMETHYL glutaric ACYL COENZYME A REDUCTASE degradation protein 1
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ACYL RADICAL CYCLISATIONS 被引量:1
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作者 陈立功 孙春光 王多禄 《Transactions of Tianjin University》 EI CAS 1996年第2期71+65-70,共7页
Several phenylselenyl esters were selected as precursors of acyl radicals and synthesised by our established method. Treatment of these phenylselenyl esters with tributyltinhydride and AIBN ge... Several phenylselenyl esters were selected as precursors of acyl radicals and synthesised by our established method. Treatment of these phenylselenyl esters with tributyltinhydride and AIBN generated the corresponding acyl radicals, which were found to proceed in 5 exo trig or 6 endo trig cyclisations to afford high yields of cyclopentanones and cyclohexanones, The substitution groups on the substrates have played an important role on the regioselectivity of cyclisations. 展开更多
关键词 phenylselenyl ester acyl radical 5 exo trig process 6 endo trig process regioselectivity
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Two new C-glycosylflavones from the leaves of Iris lactea var. chinensis 被引量:3
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作者 Wen Juan Shen Min Jian Qin +1 位作者 Pan Shu Chao Feng Zhang 《Chinese Chemical Letters》 SCIE CAS CSCD 2008年第7期821-824,共4页
Two new acylated C-glycosylflavones were isolated from the leaves of Iris lactea var. chinensis, and their structures were elucidated on the basis of extensive NMR experiments and mass spectrometry methods and were as... Two new acylated C-glycosylflavones were isolated from the leaves of Iris lactea var. chinensis, and their structures were elucidated on the basis of extensive NMR experiments and mass spectrometry methods and were assigned as 5-hydroxy-4'- methoxyflavone-7-O-(β-D-2''''4''''-diacetylrhamnopyranosyl)-6-C-[ O-(α-L-6'''-acetyl-glucpyranosyl)-1→2-β-D-glucopyrano- side] (irislactin A) and 5-hydroxy-4',7-dimethoxyflavone-6-C-[O-(α-L-2''',3'''-diacetylrhanmo-pyranosyl)-1→ 2-β-D-glucopyranoside] (irislactin B). 展开更多
关键词 Iris lactea IRIDACEAE acylated C-glycosylflavone
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Comparison of total/active ghrelin levels in primary open angle glaucoma,pseudoexfoliation glaucoma and pseudoexfoliation syndrome 被引量:3
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作者 Numan Eraslan Ufuk Elgin +2 位作者 Emine Sen Aytul Kilic Pelin Yilmazbas 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第5期823-827,共5页
AIM:To investigate the levels of ghrelin(Gh),acylated ghrelin(AGh) and AGh/Gh ratio in the humor aqueous(HA) of cases with pseudoexfoliation syndrome(PXS),pseudoexfoliation glaucoma(PXG),primary open angle ... AIM:To investigate the levels of ghrelin(Gh),acylated ghrelin(AGh) and AGh/Gh ratio in the humor aqueous(HA) of cases with pseudoexfoliation syndrome(PXS),pseudoexfoliation glaucoma(PXG),primary open angle glaucoma(POAG) and to compare these with control subjects.METHODS:A prospective examination was made of the total Gh,and AGh levels in HA of 67 patients undergoing cataract surgery.Patients were divided into 4 groups.HA samples were aspirated at the beginning of the surgery,stored at -70℃.Gh and AGh quantification was performed with ELISA kits and the AGh/total-Gh ratios were calculated.ANOVA,Kruskal-Wallis,Chi-square and post-hoc tests were used for statistical analysis.RESULTS:Total Gh levels in HA were 189.2±45.6 pg/mL in the control group,199.2±32.9 pg/mL in PXS,180.6±20.9 pg/mL in PXG and 176.8±21.4 pg/mL in POAG groups(P〉0.05).AGh levels in HA were 23.09±5.01 pg/mL in the control group,24.13±5.22 pg/mL in PXS,22.29±1.55 pg/m L in PXG and 19.69±2.93 pg/mL in POAG groups(P〉0.05).The ratio of AGh/Gh was 10.3%±2.34% in the control group,13.03%±2.58% in PXS,12.3%±1.54% in PXG and 11.79%±1.41% in POAG groups(P=0.044).The difference between the PXS and control groups was significant(P=0.03).CONCLUSION:In spite of statistically insignificant results,the HA total Gh levels were lower than those of the control subjects but not parallel with the AGh levels in glaucoma patients.The relative increase in the AGh/Gh ratio in glaucoma cases supports the view that proportional increases of AGh might play a role in the pathogenesis of glaucoma. 展开更多
关键词 GHRELIN acylated ghrelin humour aqueous pseudoexfoliation syndrome pseudoexfoliation glaucoma primary open angle glaucoma
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Synthesis and characterization of N-3-phenyl-2-propenoyl amino acids
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作者 WANG Jun-ling LIU Jin-gui LI Gong-an MIAO Chang-qing 《合成化学》 CAS CSCD 2004年第z1期69-69,共1页
关键词 acylate animo ACIDS 3-phenyl-2-propenoyl CHLORIDE SYNTHESIS
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A magnetic biocatalyst based on mussel-inspired polydopamine and its acylation of dihydromyricetin 被引量:4
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作者 邓啸 曹诗林 +4 位作者 李宁 吴虹 Thomas J.Smithd 宗敏华 娄文勇 《Chinese Journal of Catalysis》 SCIE EI CAS CSCD 北大核心 2016年第4期584-595,共12页
A support made of mussel-inspired polydopamine-coated magnetic iron oxide nanoparticles (PD-MNPs) was prepared and characterized. The widely used Aspetyillus niger lipase (ANL) was immobilized on the PD-MNPs (ANL... A support made of mussel-inspired polydopamine-coated magnetic iron oxide nanoparticles (PD-MNPs) was prepared and characterized. The widely used Aspetyillus niger lipase (ANL) was immobilized on the PD-MNPs (ANL@PD-MNPs) with a protein loading of 138 mg/g and an activity recovery of 83.6% under optimized conditions. For the immobilization, the pH and immobilization time were investigated. The pH and thermal and storage stability of the ANL@PD-MNPs significant- ly surpassed those of free ANL. The ANL@PD-MNPs had better solvent tolerance than free ANL. The secondary structure of free ANL and ANL@PD-MNPs was analyzed by infrared spectroscopy, A kinetic study demonstrated that the ANL@PD-MNPs had enhanced enzyme-substrate affinity and high catalytic efficiency. The ANL@PD-MNPs was applied as a biocatalyst for the regioselective acylation of dihydromyricetin (DMY) in DMSO and gave a conversion of 79.3%, which was higher than that of previous reports. The ANL@PD-MNPs retained over 55% of its initial activity after 10 cycles of reuse. The ANL@PD-MNPs were readily separated from the reaction system by a magnet. The PD-MNPs is an excellent support for ANL and the resulting ANL@PD-MNPs displayed good potential for the efficient synthesis of dihydromyricetin-3-acetate by enzymatic regioselective acylation. 展开更多
关键词 Magnetic iron oxide Nanoparticle POLYDOPAMINE Aspergillus niger lipase DIHYDROMYRICETIN ACYLATION
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法国公司推出防治母猪尿道和生殖道疾病的营养方案
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作者 邹晓文 《中国动物保健》 2005年第1期28-28,共1页
母猪尿道和生殖系统的疾病是生猪饲养当中经常发生的问题,不管从经济上还是技术上,都会导致一些饲养者无法忽视的后果。在法国,根据饲养的类型不同,这类疾病所导致的损失每百头母猪高达一千五百到两千欧元。因病症出现很晚,这类疾... 母猪尿道和生殖系统的疾病是生猪饲养当中经常发生的问题,不管从经济上还是技术上,都会导致一些饲养者无法忽视的后果。在法国,根据饲养的类型不同,这类疾病所导致的损失每百头母猪高达一千五百到两千欧元。因病症出现很晚,这类疾病又很难检测,因此提高尿液的酸性程度,能够起到限制细菌生长的作用,是一种有效的预防措施。 展开更多
关键词 法国 母猪 尿道疾病 生殖道疾病 营养方案 ACYL
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Anti-oxidant and anti-inflammatory effects of hydrogenrich water alleviate ethanol-induced fatty liver in mice 被引量:14
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作者 Ching-Pin Lin Wen-Chen Chuang +1 位作者 Fung-Jou Lu Chih-Yen Chen 《World Journal of Gastroenterology》 SCIE CAS 2017年第27期4920-4934,共15页
AIM To investigate the effects of hydrogen-rich water(HRW) treatment on prevention of ethanol(Et OH)-induced early fatty liver in mice.METHODS In vitro reduction of hydrogen peroxide by HRW was determined with a chemi... AIM To investigate the effects of hydrogen-rich water(HRW) treatment on prevention of ethanol(Et OH)-induced early fatty liver in mice.METHODS In vitro reduction of hydrogen peroxide by HRW was determined with a chemiluminescence system. Female mice were randomly divided into five groups: control,Et OH,Et OH + silymarin,Et OH + HRW and Et OH + silymarin + HRW. Each group was fed a Lieber-De Carli liquid diet containing Et OH or isocaloric maltose dextrin(control diet). Silymarin was used as a positive control to compare HRW efficacy against chronic Et OH-induced hepatotoxicity. HRW was freshly prepared and given at a dosage of 1.2 m L/mouse trice daily. Blood and liver tissue were collected after chronic-binge liquid-diet feeding for 12 wk.RESULTS The in vitro study showed that HRW directly scavenged hydrogen peroxide. The in vivo study showed that HRW increased expression of acyl ghrelin,which was correlated with food intake. HRW treatment significantly reduced Et OH-induced increases in serum alanine aminotransferase,aspartate aminotransferase,triglycerol and total cholesterol levels,hepatic lipid accumulation and inflammatory cytokines,including tumor necrosis factor-alpha(TNF-α) and interleukin(IL)-6. HRW attenuated malondialdehyde level,restored glutathione depletion and increased superoxide dismutase,glutathione peroxidase and catalase activities in the liver. Moreover,HRW reduced TNF-α and IL-6 levels but increased IL-10 and IL-22 levels.CONCLUSION HRW protects against chronic Et OH-induced liver injury,possibly by inducing acyl ghrelin to suppress the pro-inflammatory cytokines TNF-α and IL-6 and induce IL-10 and IL-22,thus activating antioxidant enzymes against oxidative stress. 展开更多
关键词 Hydrogen Chronic plus binge Et OH feeding Antioxidant Protective cytokine Acyl ghrelin Female mice
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