Changes in Florets’Vertical Direction within Inflorescence Affects Pollinator Behavior,and Fitness in Trifolium repens

interactions,many flowers are known to display their attractive qualities,such as scent emission,flower rewards and floral vertical direction,in a rhythmic fashion.However,less is known about how plants regulate the relationship between these flower traits to adapt to pollinator visiting behavior and increase reproduction success.Here we investigated the adaptive significance of the flower bending from erect to downward in Trifolium repens.We observed the flowering dynamic characteristics(changes of vertical direction of florets,flowering number,pollen grain numbers,pollen viability and stigma receptivity over time after blossom)and the factors affecting the rate of flower bending in T.repens.Then we altered the vertical direction of florets in inflorescence of different types(upright and downward),and compared the pollinator behaviors and female reproductive success.Our results showed that florets opened sequentially in inflorescence,and then bend downwards slowly after flowering.The bending speed of florets was mainly influenced by pollination,and bending angle increased with the prolongation of flowering time,while the pollen germination rate,stigma receptivity and nectar secretion has a rhythm of“lowhigh-low”during the whole period with the time going.The visiting frequency of all the four species of pollinators on upward flowers was significantly higher than that of downward flowers,and they especially prefer to visit flowers with a bending angle of 30°–60°,when the flowers was exactly of the highest flower rewards(nectar secretion and number of pollen grains),stigma receptivity and pollen germination rate.The seed set ratio and fruit set ratio of upward flowers were significantly higher than downward flowers,but significantly lower than unmanipulated flowers.Our results indicated that the T.repens could increase female and male fitness by accurate pollination.The most suitable flower angle saves pollinators’visiting energy and enables them to obtain the highest nectar rewards.This coordination between plants and pollinators maximizes the interests of them,which is a crucial factor in initiating specialized plant-pollinator relationships.

High capacity reversible data hiding in encrypted images based on adaptive quadtree partitioning and MSB prediction

To improve the embedding capacity of reversible data hiding in encrypted images(RDH-EI),a new RDH-EI scheme is proposed based on adaptive quadtree partitioning and most significant bit(MSB)prediction.First,according to the smoothness of the image,the image is partitioned into blocks based on adaptive quadtree partitioning,and then blocks of different sizes are encrypted and scrambled at the block level to resist the analysis of the encrypted images.In the data embedding stage,the adaptive MSB prediction method proposed by Wang and He(2022)is improved by taking the upper-left pixel in the block as the target pixel,to predict other pixels to free up more embedding space.To the best of our knowledge,quadtree partitioning is first applied to RDH-EI.Simulation results show that the proposed method is reversible and separable,and that its average embedding capacity is improved.For gray images with a size of 512×512,the average embedding capacity is increased by 25565 bits.For all smooth images with improved embedding capacity,the average embedding capacity is increased by about 35530 bits.