Adipocytokine profile in children with Kawasaki disease

This letter to editor discusses on the publication on adipocytokine profile in children with Kawasaki disease.Concerns on confounding factors are raised and discussed.

Changes of adipocytokine expression after diabetic rats received sitagliptin and the molecular mechanism

Objective:To study the effect of sitagliptin on adipocytokines expression in diabetic rats and its molecular mechanism.Methods:Male SD rats were chosen and randomly divided into NC group,T2DM group,SP group and SP+LY group.NC group received conventional breeding,T2DM group,SP group and SP+LY group received intraperitoneal injection of streptozotocin after 12 weeks of high-fat diet to establish diabetes animal model,SP group received sitagliptin intervention and SP+LY group received sitagliptin combined with PI3 K inhibitor LY294002 intervention.6 weeks after the intervention,serum was collected to determine the levels of biochemical indexes and adipocytokines,and visceral adipose tissue was collected to determine expression levels of adipocytokines.Results:Serum TC,TG,LDL-C,FBG,FINS,Leptin and Chemerin levels as well as HOMA-IR of T2DM group were higher than those of NC group,and HDL-C,Adiponectin and Omentin-1 levels were significantly lower than those of NC group; serum TC,TG,LDL-C,FBG,FINS,Leptin and Chemerin levels as well as HOMA-IR of SP group were lower than those of T2DM group,and HDL-C,Adiponectin and Omentin-1 levels were significantly higher than those of T2DM group; Leptin and Chemerin levels in serum and visceral adipose tissue of SP+LY group were higher than those of SP group while Adiponectin and Omentin-1 levels were significantly lower than those of SP group.Conclusion:Sitagliptin can regulate the expression of adipocytokines in adipose tissue of diabetic rats through PI3K-AKT pathway.

Exploration of the Relationship between Adipocytokines, Tradition Risk Markers, Nontraditional Risk Markers and Anthropometric Measurements in T2DM Patients

Obesity is commonly associated with type 2 diabetes and vascular disease. Changes in body composition in the obese state lead to a dysregulation of secretion of adipocyte-secreted hormones known as adipokines. The current study aimed to assess the relative physiological correlation of adipocytokines with immunity in urban Saudi patients. The serum adipocytokine (leptin, adiponectin, resistin, visfatin and apelin), metabolic parameters (insulin, fasting glucose, HbA1c % (Glycated Hemoglobin) immunological indices (IgG, IgA, IgM and IgE) and complement factors (C3, C4) in different metabolic disorders states such as obesity and T2DM (Type 2 Diabetes Mellitus) were determined. A total 100 adult male subjects were enrolled including 30 healthy that served as a control, 25 Glucophage treated T2DM, 22 overweight (Body Mass Index (BMI) ≥ 25 - 29.99) and 23 obese (BMI ≥ 30) patients. The current results showed that serum adipocytokines status has altered in obesity and treated T2DM compared to healthy individuals. In addition to HbA1c %, serum visfatin was also the prominent biomarker adipokine in treated T2DM while leptin was the highest in obese (BMI ≥ 30). These metabolic disorders did not affect serum levels of the assessed immunity indices. Current knowledge suggests that adipokines provide potential therapeutic targets against type 2 diabetes and vascular disease. This study provides a strong association between adipocytokine and IR (Insulin Resistance). With the increasing epidemic of obesity and T2DM in Saudi Arabia, these adipocytokine markers that integrate metabolic and inflammatory signals may play important roles in the treatment and prevention of obesity and diabetes as well as planning of therapeutic strategies and the early detection of diabetes.

Quantification of Steroid Receptors Gene Expression in Breast Cancer Patients: Possible Correlation with Serum Level of Adipocytokines

Background: While the role of adipocyte-derived hormones in breast cancer pathogenesis well studied, it is speculated that there is a crosstalk between adipocytokines and Esterogen receptor(ER) signaling. To test this hypothesis we evaluate the possible correlation between serum levels of adipocytokins with steroid hormone receptors gene expression in breast cancer patients. Methods: In this case-control study, 70 women with breast cancer participated with different grades of obesity (36 none obese, BMI 0.05). In case of leptin, we observed a positive correlation between leptin and ERα and PR mRNA. (r = 0.553, n = 64, p < 0.001, r = 0.359, n = 64, p = 0.002 and a reverse significant correlation between leptin and ERβ (r= –0.506, n = 64 and p < 0.001). We also observed a significant correlation between TNFα and IL6 with steroid receptors gene expression in samples (P < 0.05). Conclusion: As the presence of steroid receptors are used as the most common prognostic markers in breast cancer patients, so targeting adipocytokines as potential regulators of steroid receptorsgene expression can be a new approach in treatment of obese breast cancer subjects.

Adipocytokines and psoriasis: Insights into mechanisms linking obesity and inflammation to psoriasis

Psoriasis has been lately seen as a potential systemic inflammatory disease associated with a range of comorbidities exhibiting an overlapping pathology and presenting a great social health impact such as cardiovascular disease and metabolic diseases,including obesity.Adipose tissue is considered a genuine endocrine organ producing a variety of bioactive adipocytokines,like leptin,adiponectin,resistin and visfatin,participating in physiological and pathological processes,such as energy balance,insulin sensitivity and resistance,immunity,inflammation,hematopoiesis and angiogenesis.Adipocytokines could serve as a missing link in the association between psoriasis,obesity and metabolic co-morbidities.In chronic inflammatory disease states such as psoriasis,adipocytokines may be implicated in psoriasis onset,progression,severity as well as in the pathogenesis of co-morbidities.Measuring serum adipocytokine levels in the future may be useful in predicting psoriasis severity,progression,treatment outcome and risk of any co-mor-bidities.Interventions to decrease pro-inflammatory adipocytokine levels could offer preventive and therapeutic options for improving psoriasis severity and protecting against its co-morbidities.Candidate strategic interventions incorporate increased physical activity,weight control and pharmacologic approaches such as metformin.However,the mechanisms underlying the actions of adipocytokines in psoriasis as well as their potential diagnostic,prognostic and/or therapeutic utility require further investigation with larger prospective,longitudinal and mechanistic studies.

Adipocytokine profile in children with Kawasaki disease at a mean follow-up period of 5.5 years: A study from North India

BACKGROUND Kawasaki disease(KD)is an acute self-limited vasculitis with a predilection for coronary arteries.Children with KD may have altered lipid metabolism and abnormal lipid profiles that may last for prolonged periods.However,there is a paucity of literature on the role of adipocytokines in KD.AIM To estimate the levels of adipocytokines(adiponectin,leptin and resistin)during the convalescent phase of KD.METHODS Twenty children,who had KD at least three years earlier,were enrolled in this study.In addition,20 healthy controls were also enrolled.Clinical and laboratory profiles of patients were obtained from hospital records.Serum adiponectin,leptin and resistin levels were estimated by enzyme-linked immunosorbent assay.RESULTS Mean age of the patients in the study group was 10.15±3 years and the male:female ratio was 1.5:1.Median serum resistin levels in patients with KD(27.77 ng/mL;[IQR:18.66,48.90])were decreased compared to controls(21.20 ng/mL;[IQR:14.80,27.00])(P=0.04).Median serum leptin levels in cases and controls were 1.83 ng/mL;(IQR:1.13,3.80),and 1.10 ng/mL;(IQR:0.41,2.88),respectively(P=0.09).Median serum adiponectin levels were similar in both cases(12.20μg/mL;[IQR:9.76,17.97])and controls(13.95μg/mL;[IQR:11.17,22.58]);(P=0.18).There was no significant difference in all 3 adipocytokines between children with(4/20)and without coronary artery abnormalities(16/20).CONCLUSION Serum resistin levels were significantly elevated in patients with KD during the convalescent phase compared to controls.Serum leptin levels appeared to be higher in patients with KD,although the difference was not statistically significant.Adiponectin levels were similar in both cases and controls.Raised resistin and leptin levels may partially explain lipid perturbations observed during the convalescent phase of KD.

Role of Estradiol, Progestins, Insulines and Adipocytokines in Breast Cancer Promotion in Post-Menopausal Women

Estrogens and artificial progestins used in hormone replacement therapy increase breast cancer risk. This seems to bedue to a promoting and not initiating effect. A synergic effect of estradiol and hyperinsulinism has been shown. Insulinplays a role in the increase of breast cancer risk when associated with android obesity, sedentariness, type II diabetes,and high glycemic index food, alcohol and trans fatty acids intake. Natural menopause induces insulin resistance anddoes not induce a risk decrease. The role of insulin gives a new outlook on the influence of HRT in breast cancer promotion:estradiol alone, which improves insulin-sensitivity, does not increase breast cancer risk. Artificial progestinsassociated with estrogens increase the risk, whereas estrogens associated with progesterone do not. This could be dueto the fact that artificial progestins increase insulin resistance, whereas natural progesterone does not. Adipose tissue,which is an endocrine gland, is insulin dependant. Breast cancer and its seriousness are correlated to adipocytokincirculating levels such as resistin, leptin, interleukin 1, adipocyte fatty acid-binding protein, and are inversely correlatedto the level of adiponectin. Insulin could play a synergic role with sexual steroids by a direct effect and by increasingadipose tissue secretions.

Differentially expressed genes in adipocytokine signaling pathway of adipose tissue in pregnancy

Objective: To profile the differential gene expression of the KEGG Adipocytokine Signaling pathway in omental compared to subcutaneous tissue in normal pregnancy. Study Design: Subjects included 14 nonobese, normal glucose tolerant, healthy pregnant women. Matched omental and subcutaneous tissue were obtained at elective cesarean delivery. Gene expression was evaluated using microarray and validated by RT-PCR. Differential gene expression was defined as ≥1.5 fold increase at p < 0.05. Results: Six genes were significantly downregulated with two upregulated genes in omental tissue. Downregulation of Adiponectin and Insulin Receptor substrate, key genes mediating insulin sensitivity, were observed with borderline upregulation of GLUT-1. There were downregulations of CD36 and acyl-CoA Synthetase Long-chain Family Member 1which are genes involved in fatty acid uptake and activation. There was a novel expression of Carnitine palmitoyltransferase 1C. Conclusion: Differential gene expression of Adipocytokine Signaling Pathway in omental relative to subcutaneous adipose tissue in normal pregnancy suggests a pattern of insulin resistance, hyperlipidemia, and inflammation.

Correlation of sex hormones with adipocytokines and bone metabolism biochemical indexes in women with menopausal osteoporosis

Objective:To investigate the correlation of sex hormones with adipocytokines and bone metabolism biochemical indexes in women with menopausal osteoporosis.Methods: A total of 118 female patients who were diagnosed with menopausal osteoporosis in the West China Second University Hospital of Sichuan University between September 2015 and October 2017 were selected as menopausal osteoporosis group, and 100 menopausal women with normal bone density who received medical examination in the West China Second University Hospital of Sichuan University during the same period were selected as normal control group. The contents of sex hormones, adipocytokines and bone metabolism biochemical indexes in peripheral blood were compared between two groups of women, and Pearson test was used to evaluate the correlation between sex hormones and disease severity in women with menopausal osteoporosis.Results: E2 content in the peripheral blood of menopausal osteoporosis group was significantly lower than that of control group whereas FSH and LH contents were not significantly different from those of control group;Resistin and chemerin contents in peripheral blood were higher than those of control group whereas LEP and Visfatin contents were lower than those of control group;OC, NBAP, TRACP and ICTP contents in peripheral blood were significantly higher than those of control group. Pearson test showed that the sex hormone E2 content in women with menopausal osteoporosis was directly correlated with the contents of adipocytokines and bone metabolism biochemical indexes.Conclusion: The sex hormone expression is abnormal in women with menopausal osteoporosis, and the specific abnormal level can objectively reflect the condition of osteoporosis.

Correlation of visceral adipose tissue content with insulin signal transduction, lipid metabolism features and adipocytokine secretion in patients with T2DM

Objective: To study the correlation of visceral adipose tissue content with insulin signal transduction, lipid metabolism features and adipocytokine secretion in patients with type 2 diabetes mellitus (T2DM). Methods: The patients who were first diagnosed as T2DM in our hospital between March 2015 and February 2018 were selected as the T2DM group, and the healthy subjects who underwent physical examination during the same period were selected as the control group. The T2DM group underwent visceral adipose tissue content scanning and were divided into those with VAT 100 cm2 and those with VAT<100 cm2. Fasting peripheral blood was collected from the two groups to measure the F-Ins, lipid metabolism indexes and adipocytokines and calculate the HOMA-IR and ISI. Results: F-Ins and HOMA-IR levels as well as LDL-C, Apo-B, FFA, LP and RBP4 contents of T2DM group were significantly higher than those of control group whereas ISI level as well as HDL-C, Apo-A, APN and Omentin-1 contents was significantly lower than those of control group;F-Ins and HOMA-IR levels as well as of LDL-C, Apo-B, FFA, LP and RBP4 contents of T2DM group of patients with VAT 100 cm2 were higher than those of patients with VAT<100 cm2 whereas ISI level as well as HDL-C, Apo-A, APN and Omentin-1 contents was lower than those of patients with VAT<100 cm2. Conclusion: The increase of visceral adipose tissue content in patients with T2DM can aggravate the insulin resistance and cause the disorder of lipid metabolism and adipocytokine secretion.

Effect of high-intensity interval training on blood glucose control, adipocytokine secretion and oxidative stress response in patients with T2DM

Objective:To investigate the effect of high-intensity interval training on blood glucose control, adipocytokine secretion and oxidative stress response in patients with T2DM.Methods: A total of 92 patients with newly diagnosed T2DM who were treated in this hospital between July 2016 and July 2017 were divided into the control group (n=46) and HIIT group (n=46) by random number table method. Control group received conventional hypoglycemic therapy, HIIT group received hypoglycemic combined with high-intensity interval training therapy, and the intervention lasted for 3 months. The differences in blood glucose control, adipocytokine secretion and oxidative stress response were compared between the two groups before and after intervention.Results: Before intervention, the differences in blood glucose index levels in peripheral blood as well as the contents of adipocytokines and oxidative stress indexes in serum were not statistically significant between the two groups of patients. After 3 months of intervention, blood glucose indexes FPG, FINS and 2hPBG levels in peripheral blood of HIIT group were lower than those of control group;adipocytokine APN content in serum was higher than that of control group whereas LEP, Resistin and CHEM contents were lower than those of control group;oxidative stress indexes MDA and ROS contents in serum were lower than those of control group whereas T-AOC content was higher than that of control group. Conclusion: Routine hypoglycemic therapy combined with high-intensity interval training can further control the blood glucose levels, regulate the secretion of adipocytokines and reduce the systemic oxidative stress response.

Correlation of serum ADAMTS-1 and ADAMTS-13 contents with plaque nature, inflammatory factors and adipocytokines in patients with coronary heart disease

Objective: To investigate the correlation of serum ADAMTS-1 and ADAMTS-13 contents with plaque nature, inflammatory factors and adipocytokines in patients with coronary heart disease. Methods: A total of 106 patients with coronary heart disease undergoing examination in the hospital between September 2014 and June 2017 were enrolled in coronary heart disease group, and 100 healthy volunteers were enrolled in normal control group. Differences in serum ADAMTS-1, ADAMTS-13, plaque nature-related index, inflammatory factor and adipocytokine contents were compared between the two groups. Pearson test was used to assess the correlation of ADAMTS-1 and ADAMTS-13 contents with plaque nature, inflammatory factor and adipocytokines in patients with coronary heart disease. Results: Serum ADAMTS-1 content of coronary heart disease group was higher than that of normal control group whereas ADAMTS-13 content was lower than that of normal control group;serum plaque nature-related indexes Hcy and UA contents were higher than those of normal control group;serum inflammatory factors HMGB1, IL-6, IL-8 and IL-18 contents were higher than those of normal control group;serum adipocytokines APN and Vaspin contents were lower than those of normal control group whereas Visfatin and RBP4 contents were higher than those of normal control group. Pearson test showed that serum ADAMTS-1 and ADAMTS-13 contents of patients with coronary heart disease were directly correlated with the contents of plaque nature-related indexes, inflammatory factors and adipocytokines. Conclusion: Serum ADAMTS-1 content abnormally increases and ADAMTS-13 content abnormally decreases in patients with coronary heart disease, and the specific contents are directly correlated with the condition of coronary heart disease.

Correlation of second-trimester serum 25-OH-VitD3 with inflammatory factors and adipocytokines in patients with gestational diabetes mellitus

Objective: To investigate the correlation of second-trimester serum 25-OH-VitD3 with inflammatory factors and adipocytokines in patients with gestational diabetes mellitus. Methods: A total of 92 second-trimester pregnant women who were diagnosed with GDM in this hospital between December 2014 and January 2017 were selected as GDM group, and 80 healthy pregnant women who received antenatal care in this hospital during the same period were selected as normal control group. The serum levels of 25-OH-VitD3, inflammatory factors and adipocytokines were compared between the two groups of pregnant women, and Pearson test was used to evaluate the correlation of serum 25-OH-VitD3 level with inflammatory factors and adipocytokines in patients with GDM. Results: Serum 25-OH-VitD3 level of GDM group was lower than that of normal control group;serum inflammatory factors TNF-α, CRP and IL-6 levels were higher than those of normal control group while IL-10 level was lower than that of normal control group;serum adipocytokines RBP4 and VF levels were higher than those of normal control while APN level was lower than that of normal control group. Second-trimester serum 25-OH-VitD3 level of patients with GDM was directly correlated with the contents of inflammatory factors and adipocytokines. Conclusion: Second-trimester 25-OH-VitD3 deficiency in patients with GDM may be involved in the development of GDM through inflammatory factors and adipocytokines.

Correlation of serum SFRP5 and Klotho expression with oxidative stress and adipocytokine secretion in patients with diabetic nephropathy

Objective: To investigate the correlation of serum SFRP5 and Klotho expression with oxidative stress and adipocytokine secretion in patients with diabetic nephropathy. Methods: A total of 180 patients with DN were selected as DN group and 100 healthy volunteers were selected as normal control group. The differences in serum SFRP5 and Klotho expression as well as oxidative stress index and adipocytokine levels were compared between the two groups. Pearson test was used to evaluate the correlation of serum SFRP5 and Klotho expression with oxidative stress and adipocytokine secretion in DN patients. Results: Serum SFRP5 and Klotho levels of DN group were lower than those of normal control group;oxidative stress indexes MDA and LHP levels were higher than those of normal control group whereas SOD and CAT levels were lower than those of control group;adipocytokines Visfatin, Nesfatin-1 and APN levels were lower than those of normal control group whereas LEP level was higher than that of normal control group. Pearson test showed that serum SFRP5 and Klotho expression in patients with DN were directly correlated with the oxidative stress index levels and adipocytokine secretion. Conclusion: Serum SFRP5 and Klotho expression are extremely low in patients with DN, and the specific expression can objectively reflect the oxidative stress extent and the abnormal secretion of adipocytokines.

Effect of rosuvastatin intensification therapy on blood lipid metabolism,adipocytokines and plaque stability after PCI in ACS patients

Objective: To explore the effect of rosuvastatin intensification therapy on blood lipid metabolism, adipocytokines and plaque stability after PCI in ACS patients. Methods: ACS patients who received PCI in the hospital between July 2015 and January 2017were reviewed and divided into the routine dose group (n=60) who received rosuvastatin routine dose therapy after PCI and the intensification therapy group (n=46) who received rosuvastatin intensification therapy after PCI. The differences in blood lipid metabolism, adipocytokines and plaque stability were compared between the two groups before and after treatment. Results: Before PCI, the differences in blood lipid metabolism, adipocytokines and plaque stability were not statistically significant between the two groups. 1 month after PCI, lipid metabolism indexes HDL-C and ApoA1 levels in peripheral blood of intensification therapy group were higher than those of routine dose group while LDL-C and ApoB levels were lower than those of routine dose group;adipocytokines APN and Leptin levels in serum were higher than those of routine dose group while Resistin level was lower than that of routine dose group;plaque stability-related indexes ICAM-1, MMP-1 and TIMP-1 levels were lower than those of routine dose group. Conclusion: Rosuvastatin intensification therapy after PCI could effectively regulate the lipid metabolism and increase the plaque stability in ACS patients.

Relationships of body composition and adipocytokines with outcomes in metastatic castration-resistant prostate cancer patients receiving docetaxel chemotherapy

We evaluated the relationships of body composition and serum adipocytokine levels with progression-free survival(PFS)and overall survival(OS)in metastatic castration-resistant prostate cancer(mCRPC)patients receiving docetaxel.The medical records of mCRPC patients who received docetaxel between January 2011 and December 2015 at Fudan University Shanghai Cancer Center(Shanghai,China)were reviewed.The following body composition parameters were calculated using computed tomography:skeletal muscle index(SMI),visceral adipose tissue index(VATI),and subcutaneous adipose tissue index(SATI).Pretreatment serum adipocytokine levels,including interleukin 6,insulin,leptin,monocyte chemoattractant protein-1,adiponectin,and resistin,were measured using the multiplex bead-based immunoassays.Cox regression and Kaplan–Meier methods were used for survival analyses.Of the 453 mCRPC patients initially identified,105 were included in the analysis.High VATI group patients had longer PFS(median,10 months vs 7 months,P=0.008)and OS(median,24 months vs 15 months,P=0.017),compared with low VATI group patients.SMI and SATI were not significantly associated with PFS or OS.Of the six detected adipocytokines,only leptin was associated with mCRPC prognosis.High leptin group patients had shorter PFS(median,7 months vs 12 months,P=0.0018)and OS(median,17 months vs 22 months,P=0.042),compared with low leptin group patients.Multivariate analysis showed that a high VATI was an independent protective factor for PFS and OS,while a high leptin level was an independent risk factor for PFS and OS.Therefore,VATI and serum leptin levels could provide important information concerning mCRPC prognosis.

创伤性膝骨关节炎患者血清CX3CL1、Apelin-13与炎症指标的相关性及预后价值

目的 探索创伤性膝骨关节炎(PTOA)患者血清趋化因子CX3CL1及脂肪细胞因子-13(Apelin-13)的表达水平,并分析二者与炎症指标的相关性及预后价值。方法 选取2020年1月至2022年2月在该院确诊的65例PTOA患者作为试验组,选取同期来该院体检的55例体检健康者作为对照组。通过酶联免疫吸附试验(ELISA)检测血清CX3CL1与Apelin-13的表达水平。Pearson法相关性分析血清CX3CL1与Apelin-13与炎症指标的相关性。绘制受试者工作特征(ROC)曲线分析CX3CL1与Apelin-13对PTOA患者预后的诊断价值。多因素Logistic回归分析PTOA患者预后不良的影响因素。结果 与对照组相比,试验组中白细胞介素-6(IL-6)、瘦素、降钙素原(PCT)、超敏C反应蛋白(hsCRP)、肿瘤坏死因子-α(TNF-α)、CX3CL1的表达水平显著升高,差异有统计学意义(P<0.05),Apelin-13的表达水平显著下降,差异有统计学意义(P<0.05);Pearson法相关性分析显示,CX3CL1与IL-6、瘦素、TNF-α呈正相关(r=0.528、0.602、0.511,P<0.001)。Apelin-13与IL-6呈负相关(r=-0.541,P<0.001);多因素Logistic回归分析显示IL-6、瘦素、TNF-α、CX3CL1与Apelin-13是PTOA患者预后不良的影响因素(P<0.05);ROC曲线下面积(AUC)分析显示,与血清CX3CL1和Apelin-13单独指标相比,CX3CL1联合Apelin-13预测预后不良的AUC更大(Z_(联合)-CX3CL1=2.010,P=0.044;Z_(联合)-Apelin-13=2.091,P=0.036)。结论 PTOA患者血清CX3CL1表达水平显著升高,Apelin-13表达水平显著降低,CX3CL1与Apelin-13是PTOA预后不良的影响因素,二者联合检测对于PTOA的预后提供帮助。

骨髓脂肪细胞因子与骨髓增生异常综合征的相关性研究

目的:研究骨髓脂肪细胞因子与骨髓增生异常综合征(myelodysplastic syndromes,MDS)发生、进展及预后的关系。方法:回顾性分析2020年2月至2022年2月郑州大学附属肿瘤医院治疗的采用酶联免疫吸附法(ELISA)检测的72例MDS和16例MDS继发急性髓系白血病(secondary acute myeloid leukemia,sAML)患者骨髓上清液中的脂肪细胞因子,包括脂联素(adiponectin,ADP)、瘦素(leptin,LEP)、内脏脂肪素(nicotinamide phosphoribosyltransferase,NAMPT)、降脂素(complement factor D,CFD)和人补体C1q肿瘤坏死因子相关蛋白1(C1q/TNF-related protein 1,CTRP1)。对其中70例患者采用高通量测序靶向检测78种血液肿瘤相关基因,分析脂肪细胞因子与患者临床特征、疾病亚型、异常基因和预后等的关系。结果:临床特征相关结果显示,男性ADP和LEP水平分别较女性显著降低(P=0.027,P=0.019);年龄<65岁患者的ADP、CFD和NAMPT水平较年龄≥65岁患者显著降低(P=0.020,P<0.001,P=0.021),而LEP水平显著升高(P=0.043);体质量指数(BMI)<24 kg/m^(2)患者ADP水平较BMI≥24 kg/m^(2)患者显著升高(P=0.025),而LEP水平显著降低(P=0.020);原始细胞升高组的NAMPT水平较无原始细胞升高组显著升高(P=0.037);MDS组的CTRP1水平较sAML组显著升高(P=0.010)。异常基因相关分析显示,CTRP1水平的升高与表观遗传相关异常基因的发生呈正相关(P=0.001),与TET2和U2AF1的发生呈正相关(P<0.001,P=0.036);ADP和CFD水平分别与NPM1的发生呈正相关(P=0.048,P=0.026)。多因素Cox回归生存分析显示,LEP<0.2 ng/mL是MDS患者无进展生存期(progression-free survival,PFS)和总生存期(overall survival,OS)的独立危险因素(P=0.002,P<0.001),而NAMPT<2.1 ng/mL是MDS患者PFS的保护因素(P=0.043)。结论:骨髓微环境中脂肪细胞因子与MDS患者一般特征、基因突变以及预后密切相关,其中LEP<0.2 ng/mL是MDS患者独立的预后危险因素,而NAMPT<2.1 ng/mL是预后保护因素。

脂肪细胞因子通路基因多态性与肥胖儿童非酒精性脂肪性肝病的关联

目的:非酒精性脂肪性肝病(non-alcoholic fatty liver disease,NAFLD)存在明显的遗传易感性,脂肪细胞因子通过参与胰岛素抵抗和肝脏脂肪变性等过程,在NAFLD的发生和发展中发挥重要作用,但参与脂肪细胞因子通路的基因与NAFLD之间的关联仍不明确。本研究旨在探索脂肪细胞因子通路的基因多态性位点及其交互作用与肥胖儿童NAFLD的关联。方法:采用病例对照研究,将肥胖儿童分为NAFLD组和对照组。采集受试者外周静脉血2 mL,提取DNA后采用多重PCR和高通量测序对脂肪细胞因子通路的14个单核苷酸多态性(single nucleotide polymorphism,SNP)进行分型检测。采用单因素及多因素Logistic回归分析各SNP与肥胖儿童NAFLD的关联。基于显性模型,联合使用交叉分析和Logistic回归分析相加或相乘交互作用。采用广义多因子降维法(generalized multifactor dimensionality reduction,GMDR)检测14个SNP之间基因-基因交互作用与肥胖儿童NAFLD之间的关联。结果:共纳入1 022例儿童,NAFLD组与对照组各511例。在调整年龄、性别、BMI后,多因素Logistic回归结果显示:PPARG rs1801282在3个遗传模型中与肥胖儿童NAFLD存在关联,分别是杂合子模型(CG vs CC,OR=0.58,95%CI 0.36~0.95,P=0.029)、显性模型(CG+GG vs CC,OR=0.62,95%CI 0.38~1.00,P=0.049)、超显性模型(CC+GG vs CG,OR=1.72,95%CI 1.06~2.80,P=0.028);PRKAG2 rs12703159在4个遗传模型中与肥胖儿童NAFLD存在关联,分别是杂合子模型(CT vs CC,OR=1.51,95%CI 1.10~2.07,P=0.011)、显性模型(CT+TT vs CC,OR=1.50,95%CI 1.10~2.03,P=0.010)、超显性模型(CC+TT vs CT,OR=0.67,95%CI 0.49~0.92,P=0.012)、加性模型(CC vs CT vs TT,OR=1.40,95%CI 1.07~1.83,P=0.015)。但PPARG rs1801282与PRKAG2 rs12703159间的相乘及相加交互作用均与肥胖儿童NAFLD不存在关联。经GMDR分析,调整年龄、性别、BMI后,14个SNP之间的交互作用均无统计学意义(均P>0.05)。结论:PPARG rs1801282、PRKAG2 rs12703159突变型与肥胖儿童NAFLD存在关联,但未发现SNP交互作用与肥胖儿童NAFLD之间的关联。

妊娠期糖尿病孕妇血清Chemerin的变化及其与胰岛素抵抗的关系

目的探讨血清趋化素(Chemerin)在妊娠期糖尿病(GDM)孕妇中的变化,分析其与胰岛素抵抗的关系。方法选择2019年3月至2020年2月深圳市龙岗中心医院收治的50例GDM孕妇作为观察组,并选择同期接诊的血糖筛查或75 g葡萄糖耐量(OGTT)检查正常的50例孕妇作为对照组。比较两组孕妇的甘油三酯(TG)、总胆固醇(TC)、空腹血糖(FPG)、胰岛素抵抗指数(HOMA-IR)及血清Chemerin水平,并采用Pearson法分析血清Chemerin水平与HOMA-IR的相关性。结果观察组孕妇的TG、TC、FPG、HOMA-IR分别为(2.24±0.41)mmol/L、(5.30±0.68)mmol/L、(6.02±0.58)mmol/L、2.30±0.42,均明显高于对照组的(1.62±0.33)mmol/L、(4.76±0.57)mmol/L、(4.29±0.41)mmol/L、1.23±0.37,差异均有统计学意义(P<0.05);观察组孕妇的血清Chemerin水平为(8.78±1.89)mg/L,明显高于对照组的(4.54±1.21)mg/L,差异具有统计学意义(P<0.05);Pearson相关性分析结果显示,血清Chemerin水平与HOMA-IR呈正相关(r=0.619,P<0.05)。结论血清Chemerin在GDM孕妇中明显升高,且与胰岛素抵抗有明显相关性。