In this study a hovel metal ion affinity ligand was immobility onto the sensor chip. Three poly-histidine peptides were used to study the interaction of tile peptides and the immobilised metal ion affinity ligand via ...In this study a hovel metal ion affinity ligand was immobility onto the sensor chip. Three poly-histidine peptides were used to study the interaction of tile peptides and the immobilised metal ion affinity ligand via biosensor system . The results obtained in this study indicate that the affinity of immobilised Ni(Ⅱ) ion affinity ligand for these peptides appear to be related to the arrangement of the histidine residues in the peptides. This study first documents the application of biosensor technique for paptide screening.展开更多
Inorganic nanocarriers are potent candidates for delivering conventional anticancer drugs,nucleic acid-based therapeutics,and imaging agents,influencing their blood half-lives,tumor targetability,and bioactivity.In ad...Inorganic nanocarriers are potent candidates for delivering conventional anticancer drugs,nucleic acid-based therapeutics,and imaging agents,influencing their blood half-lives,tumor targetability,and bioactivity.In addition to the high surface area-to-volume ratio,they exhibit excellent scalability in synthesis,controllable shape and size,facile surface modification,inertness,stability,and unique optical and magnetic properties.However,only a limited number of inorganic nanocarriers have been so far approved for clinical applications due to burst drug release,poor target specificity,and toxicity.To overcome these barriers,understanding the principles involved in loading therapeutic and imaging molecules into these nanoparticles(NPs)and the strategies employed in enhancing sustainability and targetability of the resultant complexes and ensuring the release of the payloads in extracellular and intracellular compartments of the target site is of paramount importance.Therefore,we will shed light on various loading mechanisms harnessed for different inorganic NPs,particularly involving physical entrapment into porous/hollow nanostructures,ionic interactions with native and surface-modified NPs,covalent bonding to surface-functionalized nanomaterials,hydrophobic binding,affinity-based interactions,and intercalation through co-precipitation or anion exchange reaction.展开更多
Here, we introduce the design of the structure of RR（RYYAAFFARR）, an inhibitor of Aβ aggregation, using molecular docking, and compare the inhibitory ability between RR and LPFFD, which interacts with Aβ mainly de...Here, we introduce the design of the structure of RR（RYYAAFFARR）, an inhibitor of Aβ aggregation, using molecular docking, and compare the inhibitory ability between RR and LPFFD, which interacts with Aβ mainly depending on hydrophobic interaction. Our results showed that RR which can target multiple regions of Aβ with multiple weak interactions is better than those that only target a single region with the single driving force mainly.展开更多
文摘In this study a hovel metal ion affinity ligand was immobility onto the sensor chip. Three poly-histidine peptides were used to study the interaction of tile peptides and the immobilised metal ion affinity ligand via biosensor system . The results obtained in this study indicate that the affinity of immobilised Ni(Ⅱ) ion affinity ligand for these peptides appear to be related to the arrangement of the histidine residues in the peptides. This study first documents the application of biosensor technique for paptide screening.
文摘Inorganic nanocarriers are potent candidates for delivering conventional anticancer drugs,nucleic acid-based therapeutics,and imaging agents,influencing their blood half-lives,tumor targetability,and bioactivity.In addition to the high surface area-to-volume ratio,they exhibit excellent scalability in synthesis,controllable shape and size,facile surface modification,inertness,stability,and unique optical and magnetic properties.However,only a limited number of inorganic nanocarriers have been so far approved for clinical applications due to burst drug release,poor target specificity,and toxicity.To overcome these barriers,understanding the principles involved in loading therapeutic and imaging molecules into these nanoparticles(NPs)and the strategies employed in enhancing sustainability and targetability of the resultant complexes and ensuring the release of the payloads in extracellular and intracellular compartments of the target site is of paramount importance.Therefore,we will shed light on various loading mechanisms harnessed for different inorganic NPs,particularly involving physical entrapment into porous/hollow nanostructures,ionic interactions with native and surface-modified NPs,covalent bonding to surface-functionalized nanomaterials,hydrophobic binding,affinity-based interactions,and intercalation through co-precipitation or anion exchange reaction.
基金financially supported by the National Natural Science Foundation of China(Nos.20634030 and 51273094)State Key Fundamental R&D Project(No.2011CB606202)PCSIRT(No.IRT1257)
文摘Here, we introduce the design of the structure of RR（RYYAAFFARR）, an inhibitor of Aβ aggregation, using molecular docking, and compare the inhibitory ability between RR and LPFFD, which interacts with Aβ mainly depending on hydrophobic interaction. Our results showed that RR which can target multiple regions of Aβ with multiple weak interactions is better than those that only target a single region with the single driving force mainly.
