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Terahertz toroidal dipole metamaterial sensors for detection of aflatoxin B1
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作者 徐建伟 欧阳收剑 +4 位作者 段守鑫 邹林儿 叶丹妮 杨思嘉 邓晓华 《Chinese Physics B》 SCIE EI CAS CSCD 2024年第3期672-676,共5页
Terahertz metamaterial biosensors have attracted significant attention in the biological field due to their advantages of label-free,real-time and in situ detection.In this paper,a highly sensitive metamaterial sensor... Terahertz metamaterial biosensors have attracted significant attention in the biological field due to their advantages of label-free,real-time and in situ detection.In this paper,a highly sensitive metamaterial sensor with semi-ring mirror symmetry based on toroidal dipole resonance is designed for a new metamaterial biosensor.It is shown that a refractive index sensitivity of 337.5 GHz per refractive index unit can be achieved under an analyte of saturated thickness near a 1.33 THz transmission dip.For biosensor samples where aflatoxin B1 is dropped on the metamaterial surface in our experiment,dip amplitudes of transmission varying from 0.1904 to 0.203 and 0.2093 are observed as aflatoxin B1 concentrations are altered from 0 to 0.001μg·ml-1 and to 0.01μg·ml-1,respectively.Furthermore,when aflatoxin B1 concentrations are 0.1μg·ml-1,1μg·ml-1,10μg·ml-1 and 100μg·ml-1,dip amplitudes of 0.2179,0.226,0.2384 and 0.2527 and dip redshifts of 10.1 GHz,20.1 GHz,27.7 GHz and 37.6 GHz are respectively observed.These results illustrate high-sensitivity,label-free detection of aflatoxin B1,enriching the applications of sensors in the terahertz domain. 展开更多
关键词 TERAHERTZ METAMATERIAL toroidal dipole aflatoxin b1
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基于Nafion-CNNS复合膜构置免标记的AFB1免疫传感器
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作者 杨彩萍 《化学研究与应用》 CAS 北大核心 2024年第3期474-481,共8页
本文将氮化碳纳米片(g-C_(3)N_(4) nanosheets,CNNS)分散到一定浓度的Nafion溶液中,滴涂至玻碳电极制备修饰电极,而后将黄曲霉毒素B1抗体、黄曲霉毒素B1(AFB1)先后孵育至该修饰电极上,以鲁米诺溶液为电化学发光探针测定免疫作用前后化... 本文将氮化碳纳米片(g-C_(3)N_(4) nanosheets,CNNS)分散到一定浓度的Nafion溶液中,滴涂至玻碳电极制备修饰电极,而后将黄曲霉毒素B1抗体、黄曲霉毒素B1(AFB1)先后孵育至该修饰电极上,以鲁米诺溶液为电化学发光探针测定免疫作用前后化学发光值,依据前后发光值的差值,构置免标记的用于定量检测黄曲霉毒素B1的电致化学发光免疫传感器。结果表明,免疫结合前后鲁米诺溶液的ECL差值与溶液中黄曲霉毒素B1(AFB1)的含量在1.0量在曲^(-4)~10.0 ng·mL^(-1)和10.0~160.0 ng·mL^(-1)两个区间有着良好的线性关系,对AFB1的检出限为0.1pg·mL^(-4),该电致化学发光免疫传感器不但线性范围广,而且检出限超低,可实现对黄曲霉毒素B1的超灵敏检测。 展开更多
关键词 氮化碳纳米片(CNNS) NAFION 黄曲霉毒素b1(afb1) 电致化学发光免疫传感器 快速检测
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Tissue inhibitor of metalloproteinase-3 expression affects clinicopathological features and prognosis of aflatoxin B1-related hepatocellular carcinoma
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作者 Qiu-Ju Liang Qin-Qin Long +3 位作者 Feng-Qin Tian Qun-Ying Su Xiao-Ying Zhu Xi-Dai Long 《World Journal of Hepatology》 2024年第8期1131-1144,共14页
BACKGROUND The dysregulation of tissue inhibitor of metalloproteinase-3(TIMP3)was positively correlated with the progression of hepatocellular carcinoma(HCC).However,it is not clear whether TIMP3 expression is associa... BACKGROUND The dysregulation of tissue inhibitor of metalloproteinase-3(TIMP3)was positively correlated with the progression of hepatocellular carcinoma(HCC).However,it is not clear whether TIMP3 expression is associated with the clinico-pathological features and prognosis of aflatoxin B1(AFB1)-related HCC(AHCC).A retrospective study,including 182 patients with AHCC,was conducted to explore the link between TIMP3 expression in cancerous tissues and the clinico-pathological characteristics and prognosis of AHCC.TIMP3 expression was detected by immunohistochemistry and its effects on the clinicopathological features and prognosis of AHCC were evaluated by Kaplan-Meier survival analysis and Cox regression survival analysis.Odds ratio,hazard ratio(HR),median overall survival time(MST),median tumor recurrence-free survival time(MRT),and corresponding 95%confidential interval(CI)was calculated to RESULTS Kaplan-Meier survival analysis showed that compared with high TIMP3 expression,low TIMP3 expression in tumor tissues significantly decreased the MST(36.00 mo vs 18.00 mo)and MRT(32.00 mo vs 16 mo)of patients with AHCC.Multivariate Cox regression survival analysis further proved that decreased expression of TIMP3 increased the risk of death(HR=2.85,95%CI:2.04-4.00)and tumor recurrence(HR=2.26,95%CI:1.57-3.26).Furthermore,decreased expression of TIMP3 protein in tissues with AHCC was significantly correlated with tumor clinicopatho-logical features,such as tumor size,tumor grade and stage,tumor microvessel density,and tumor blood invasion.Additionally,TIMP3 protein expression was also negatively associated with amount of AFB1-DNA adducts in tumor tissues.CONCLUSION These findings indicate that the dysregulation of TIMP3 expression is related to AHCC biological behaviors and affects tumor outcome,suggesting that TIMP3 may act as a prognostic biomarker for AHCC. 展开更多
关键词 Tissue inhibitor of metalloproteinase-3 expression aflatoxin b1 Hepatocellular carcinoma Clinicopathological feature PROGNOSIS
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量子点荧光微球免疫法定量检测小麦中黄曲霉毒素B1
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作者 范雅靓 吴才章 赵志科 《食品安全质量检测学报》 CAS 2024年第10期208-216,共9页
目的建立量子点荧光微球免疫法快速检测小麦中黄曲霉毒素B1的方法。方法采用量子点荧光微球作为荧光标记物,与黄曲霉毒素B1的单克隆抗体偶联,构建量子点荧光微球探针。优化缓冲液pH、抗体最小标记量、荧光探针用量和包被抗原浓度等实验... 目的建立量子点荧光微球免疫法快速检测小麦中黄曲霉毒素B1的方法。方法采用量子点荧光微球作为荧光标记物,与黄曲霉毒素B1的单克隆抗体偶联,构建量子点荧光微球探针。优化缓冲液pH、抗体最小标记量、荧光探针用量和包被抗原浓度等实验条件,建立检测卡上T线和C线信号峰值面积的比值与样本中黄曲霉毒素B1浓度的关系,构建定量标准曲线。针对小麦样品,将该检测方法与时间分辨荧光定量检测方法进行比较。结果本研究建立的荧光定量免疫层析检测方法最佳反应条件为:pH 7.5磷酸钠缓冲液,抗体标记量为20μg,荧光探针用量为4.0μL,抗原质量浓度使用0.40mg/mL。小麦中黄曲霉毒素B1的定量检测线性范围为0.05~25.00μg/kg,相关系数(r^(2))为0.9994,检出限为0.02μg/kg,定量限为0.05μg/kg。加标回收率为91.50%~115.00%,变异系数为1.88%~5.46%。结论本研究建立的荧光定量免疫层析方法快速、准确、稳定性好、可靠性高,适用于小麦中黄曲霉毒素B1的现场快速检测。 展开更多
关键词 量子点荧光微球 黄曲霉毒素b1 荧光免疫 小麦
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蒲公英甾醇对AFB1所致鸡原代肝细胞氧化损伤的保护作用 被引量:1
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作者 鲁萍 王萌 +2 位作者 桑锐 王巍 张雪梅 《中国畜牧兽医》 CAS CSCD 北大核心 2023年第9期3541-3549,共9页
【目的】探讨蒲公英甾醇对黄曲霉毒素B1(AFB1)诱导的鸡原代肝细胞氧化损伤的保护作用及机制,为应用蒲公英甾醇防治AFB1中毒提供理论依据。【方法】采用组织块酶消化法分离鸡原代肝细胞并进行PAS糖原染色鉴定,通过CCK-8法绘制肝细胞生长... 【目的】探讨蒲公英甾醇对黄曲霉毒素B1(AFB1)诱导的鸡原代肝细胞氧化损伤的保护作用及机制,为应用蒲公英甾醇防治AFB1中毒提供理论依据。【方法】采用组织块酶消化法分离鸡原代肝细胞并进行PAS糖原染色鉴定,通过CCK-8法绘制肝细胞生长曲线;通过MTT法测定AFB1对鸡肝细胞的毒性,结合测定肝细胞上清液中谷丙转氨酶(ALT)和谷草转氨酶(AST)水平,以确定AFB1的体外建模浓度。通过MTT法确定蒲公英甾醇的安全给药浓度后,将试验分为6组:空白对照组、模型组、蒲公英甾醇剂量组(高、中、低剂量组)、阳性对照组。建立AFB1诱导的鸡原代肝细胞损伤模型并给予药物,通过试剂盒测定细胞内活性氧(ROS)、丙二醛(MDA)、超氧化物歧化酶(SOD)以及还原型谷胱甘肽(GSH)水平。通过实时荧光定量PCR法测定Keap1/Nrf2信号通路关键基因血红素加氧酶-1(HO-1)、NADPH醌氧化还原酶1(NQO1)、Kelch样环氧氯丙烷相关蛋白1(Keap1)和核转录因子E2相关因子2(Nrf2)表达量。【结果】试验成功分离鉴定鸡原代肝细胞,在培养24~72 h细胞活力较高;确定0.05μg/mL作为AFB1体外诱导鸡原代肝细胞损伤的建模浓度;确定20、10、5μg/mL作为蒲公英甾醇高、中、低剂量组的给药浓度;与模型组相比,蒲公英甾醇可极显著降低AFB1所致鸡原代肝细胞氧化应激相关指标ROS和MDA含量(P<0.01),极显著提高抗氧化物SOD活性(P<0.01);极显著或显著提高AFB1所致鸡原代肝细胞中HO-1、NQO1和Nrf2基因(除低剂量组外)表达量(P<0.01;P<0.05),降低Keap1基因表达量。【结论】蒲公英甾醇通过调控鸡原代肝细胞Keap1/Nrf2信号通路提高其抗氧化能力,从而对AFB1诱导的鸡原代肝细胞氧化损伤起到保护作用。 展开更多
关键词 黄曲霉毒素b1(afb1) 蒲公英甾醇 鸡原代肝细胞 氧化应激 保护作用
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乳酸菌肽聚糖对雏鸭饲粮中黄曲霉毒素B 1的脱毒效果研究
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作者 邵怡豪 王煜琦 +3 位作者 张宇豪 张广明 肖发沂 李庆梅 《动物营养学报》 CAS CSCD 北大核心 2024年第7期4339-4349,共11页
本试验旨在探究乳酸菌肽聚糖对黄曲霉毒素B_(1)(AFB_(1))的吸附作用,为乳酸菌肽聚糖的制备和饲粮中AFB_(1)的脱毒提供科学依据及理论基础。试验以4种乳酸菌(嗜酸乳杆菌、嗜热链球菌、罗伊氏乳杆菌和植物乳杆菌)为原料提取乳酸菌肽聚糖,... 本试验旨在探究乳酸菌肽聚糖对黄曲霉毒素B_(1)(AFB_(1))的吸附作用,为乳酸菌肽聚糖的制备和饲粮中AFB_(1)的脱毒提供科学依据及理论基础。试验以4种乳酸菌(嗜酸乳杆菌、嗜热链球菌、罗伊氏乳杆菌和植物乳杆菌)为原料提取乳酸菌肽聚糖,采用高效液相色谱法比较4种乳酸菌肽聚糖对AFB_(1)的体外吸附率,选取体外吸附效果最好的罗伊氏乳杆菌肽聚糖进行动物试验。选择1日龄健康樱桃谷鸭120只,随机分为5组,每组4个重复,每个重复6只。对照组饲喂基础饲粮,AFB_(1)组在基础饲粮中添加0.10 mg/kg AFB_(1),试验组在AFB_(1)组饲粮中分别添加0.10%(Ⅰ组)、0.15%(Ⅱ组)和0.20%(Ⅲ组)罗伊氏乳杆菌肽聚糖。预试期3 d,正试期21 d。结果表明:1)乳酸菌肽聚糖种类、添加量及二者的交互作用均能够显著影响AFB_(1)的体外吸附率(P<0.05),其中10.0 mg/mL罗伊氏乳杆菌肽聚糖对AFB_(1)的体外吸附率最高,达75.29%。2)与对照组相比,AFB_(1)组的平均日增重、平均日采食量显著降低(P<0.05),料重比显著升高(P<0.05);血浆谷草转氨酶(AST)、谷丙转氨酶(ALT)、碱性磷酸酶(AKP)活性及丙二醛(MDA)含量显著升高(P<0.05),肝脏、脾脏、胸腺和法氏囊指数显著升高(P<0.05),血浆免疫球蛋白G(IgG)、免疫球蛋白M(IgM)、γ-干扰素(IFN-γ)、白细胞介素-2(IL-2)、白细胞介素-4(IL-4)含量和超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及总抗氧化能力(T-AOC)显著降低(P<0.05)。3)与AFB_(1)组相比,Ⅰ、Ⅱ、Ⅲ组的平均日增重显著升高(P<0.05),料重比显著降低(P<0.05);Ⅰ、Ⅱ、Ⅲ组的血浆IgG、IFN-γ、IL-2、IL-4含量和SOD、GSH-Px活性显著升高(P<0.05),血浆AST、AKP、ALT活性及MDA含量显著降低(P<0.05);Ⅱ、Ⅲ组的胸腺和法氏囊指数显著降低(P<0.05)。由此可见,不同乳酸菌肽聚糖对AFB_(1)体外吸附效果不同,饲粮中添加罗伊氏乳杆菌肽聚糖能够部分消除AFB_(1)对雏鸭造成的生长性能下降,改善AFB_(1)所导致的免疫功能降低以及肝脏毒性。 展开更多
关键词 肽聚糖 乳酸菌 黄曲霉毒素b 1 生长性能 脱毒效果
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Dietary aflatoxin B1 induces abnormal deposition of melanin in the corium layer of the chicken shank possibly via promoting the expression of melanin synthesis-related genes
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作者 WANG Yong-li HUANG Chao +5 位作者 YU Yang CAI Ri-chun SU Yong-chun CHEN Zhi-wu ZHENG Mai-qing CUI Huan-xian 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2023年第6期1847-1856,共10页
San-Huang chicken is a high-quality breed in China with yellow feather, claw and break. However, the abnormal phenomenon of the yellow shank turning into green shank of San-Huang chicken has been a concern, as it seri... San-Huang chicken is a high-quality breed in China with yellow feather, claw and break. However, the abnormal phenomenon of the yellow shank turning into green shank of San-Huang chicken has been a concern, as it seriously reduces the carcass quality and economic benefit of yellow-feathered broilers. In this study, the cause of this abnormal green skin in shank was systematically investigated. Physiological anatomy revealed that the abnormal skin in shank was primarily due to the deposition of melanin under the dermis. After analyzing multiple potential causes such as heredity(pedigree and genetic markers), environment(water quality monitoring) and feed composition(mycotoxin detection), excessive aflatoxin B1(AFB1) in feed was screened, accompanied with a higher L-dihydroxy-phenylalanine(L-DOPA)(P<0.05) and melanin content(P<0.01). So it was speculated that excessive AFB1 might be the main cause of abnormal green skin in shank. Subsequently, the further results showed that a high concentration of AFB1(>170 μg kg–1)indeed induced the abnormal green skin in shank compared to the normal AFB1 content(<10 μg kg–1), and the mRNA levels of TYR, TYRP1, MITE, MC1R and EDN3 genes related to melanin deposition would significantly up-regulate(P<0.01) and the content and activity of tyrosinase(TyR) significantly increased(P<0.05). At the same time, the content of L-DOPA and melanin deposition also increased significantly(P<0.01), which also confirmed the effect of excessive AFB1 on melanin deposition in skin of shank. Results of additional experiments revealed that the AFB1's negative effect on melanin deposition in skin of shank could last for a longer time. Taken together, the results of this study explained the occurrence and possible mechanisms of the abnormal AFB1-related green skin in shank of chickens. Excessive AFB1 in diets increased the L-DOPA content and melanin abnormal deposition in the chicken shank possibly via promoting TyR content and activity, and the expression of melanin synthesis-related genes. Furthermore, our findings once again raised the alarm of the danger of AFB1 in the broiler production. 展开更多
关键词 aflatoxin b1 melanin deposition skin color in shank CHICKEN negative effect
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Construction of Fluorescence Sensing Platform on the Basis of Molybdenum Disulfide Nanosheet for the Detection of AFB1
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作者 Xiaoqing Wen Zichun Song +3 位作者 Jiuying Cui Yan Li Qianli Tang Xianjiu Liao 《Journal of Biosciences and Medicines》 CAS 2023年第2期1-14,共14页
Purpose: Aflatoxin B<sub>1</sub> is the most common mycotoxin in cereal crops;it is of stronger toxicity and has a carcinogenic effect. In recent years, a series of fluorescence sensors constructed on the ... Purpose: Aflatoxin B<sub>1</sub> is the most common mycotoxin in cereal crops;it is of stronger toxicity and has a carcinogenic effect. In recent years, a series of fluorescence sensors constructed on the basis of MoS<sub>2</sub>NS fluorescence quenching property have become a research hotspot. Therefore, we can construct a fast and simple analysis method with high specificity to detect AFB<sub>1</sub> by utilizing MoS<sub>2</sub>NS, which can be effectively applied to food safety monitoring and clinical diagnosis. Method: In the current research, a fluorescence biosensor is developed on the basis of a new type of two-dimensional nano-material namely MoS<sub>2</sub>NS applied for the detection of AFB<sub>1</sub>. The fluorescence of Apt@AFB<sub>1</sub> can be quickly quenched by MoS<sub>2</sub>NS through the fluorescence resonance energy transfer (FRET). When the target molecule AFB<sub>1</sub> exists, after the specificity binding between AFB<sub>1</sub> and aptamer, the Apt@AFB<sub>1</sub> loses its single stranded structure and is away from MoS<sub>2</sub>NS, and the fluorescence of Apt®AFB<sub>1</sub> cannot be quenched effectively. Such sensing signals can be used to achieve the sensitive detection of AFB<sub>1</sub>. Result: With this new method, under the optimized conditions, the AFB<sub>1</sub> is analyzed in the MoS<sub>2</sub>NS/Apt®AFB<sub>1</sub> sensing platform. Within the dynamic range of 0.2 - 25 ng/mL, the sensing platform expresses a good linear response to the level of AFB<sub>1</sub> with the R<sup>2</sup> = 0.9964 and LOD as 90 pg/mL. This method is applied to detect the actual serum samples and soybean milk with the recovery rate of 93.10% - 107.23% and 95.15% - 102.60% separately, and it can be used in the quantitative detection under the interference of other mycotoxins in a relatively accurate way. Conclusion: It is proved that this new detection method can be used as a potential biosensor platform for the detection of AFB<sub>1</sub>. This detection method features several advantages such as specificity, rapidness and low costs, which can meet the requirement of trace detection in clinical detection and food safety. 展开更多
关键词 MoSb>2b>NS APTAMER aflatoxin bb>1b> Fluorescence Sensing Analysis Food Monitoring
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HPLC法测定远志中黄曲霉毒素B1含量的不确定度评定 被引量:2
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作者 马力辉 崔炎 +1 位作者 李迅 赵志磊 《河北大学学报(自然科学版)》 CAS 北大核心 2023年第5期484-491,共8页
为评定高效液相色谱法测定远志中黄曲霉毒素B1含量的不确定度,依据国家计量技术规范JJF 1059.1—2012《测量不确定度评定与表示》,对随机选取的6个远志样品,建立数学模型,从标准溶液稀释、样品称量、样品最终定容体积和仪器测定重复性... 为评定高效液相色谱法测定远志中黄曲霉毒素B1含量的不确定度,依据国家计量技术规范JJF 1059.1—2012《测量不确定度评定与表示》,对随机选取的6个远志样品,建立数学模型,从标准溶液稀释、样品称量、样品最终定容体积和仪器测定重复性等方面分析不确定度来源,量化不确定度分量,计算合成不确定度和扩展不确定度.实验结果表明远志中黄曲霉毒素B1测定结果合成标准不确定度为0.01639μg/kg,扩展不确定度0.03278μg/kg.不确定度评定结果表明:HPLC法对远志中黄曲霉毒素B1含量的测定数据可靠,远志中黄曲霉毒素B1含量的测量不确定度主要来源于重复性实验、标准曲线的拟合、液相色谱仪的使用. 展开更多
关键词 不确定度 黄曲霉毒素b1 HPLC 远志
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用cDNA微阵列技术研究HBV X基因与AFB_1对HBVx转基因小鼠药物代谢酶基因表达谱的影响 被引量:6
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作者 张豪 孙桂菊 +3 位作者 屠红 金晏 许丽 钱耕荪 《肿瘤》 CAS CSCD 北大核心 2005年第2期128-131,共4页
目的 研究乙型肝炎病毒X基因和黄曲霉毒素诱发小鼠肝癌过程中药物代谢酶基因表达谱的变化,探讨两因素协同致肝癌的机制。方法 用BiostarM 40s微阵列芯片比较研究HBVx组、AFB1 组和(AFB1+HBVx)组的肝组织基因表达谱与对照组的差异。结... 目的 研究乙型肝炎病毒X基因和黄曲霉毒素诱发小鼠肝癌过程中药物代谢酶基因表达谱的变化,探讨两因素协同致肝癌的机制。方法 用BiostarM 40s微阵列芯片比较研究HBVx组、AFB1 组和(AFB1+HBVx)组的肝组织基因表达谱与对照组的差异。结果 各实验组分别与对照组相比基因表达谱发生了明显的改变,各实验组上调与下调的基因数目分别为(AFB1+HBVx)组69项;AFB1 组101项;HBVx组35 项;其中与代谢酶相关的基因有18 项表达发生改变,分别为(AFB1 +HBVx)组13项(13/18,72%);HBVx组4项(4/18,22%);AFB1 组8项(8/18,44%)。结论 小鼠受到HBV X基因和AFB1双重攻击后,其体内的GST、EPHX和UDPGT等药物代谢酶基因表达水平明显低于HBVx组和AFB1 组。HBV与AFB1 协同致癌的分子机制很可能与两者引起药物代谢酶基因表达水平下调有关。 展开更多
关键词 CDNA微阵列 酶类 黄曲霉毒素b1 肝炎抗原 乙型 小鼠 转基因
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有机相萃取法消除ELISA检测AFB_1假阳性的研究 被引量:11
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作者 赵春城 赵晓联 蔡正森 《饲料工业》 北大核心 2003年第3期40-43,共4页
近年来,饲料质量检验部门广泛采用酶联免疫吸附法(ELISA)检测饲料中黄曲霉毒素B_1(AFB_1)(GB/T17480—1998),但由于目前的饲料产品中添加了一定量的重金属离子及其它营养增强剂,采用国标中的提取方法易出现假阳性结果。利用有机相萃取... 近年来,饲料质量检验部门广泛采用酶联免疫吸附法(ELISA)检测饲料中黄曲霉毒素B_1(AFB_1)(GB/T17480—1998),但由于目前的饲料产品中添加了一定量的重金属离子及其它营养增强剂,采用国标中的提取方法易出现假阳性结果。利用有机相萃取法可有效地消除假阳性。 展开更多
关键词 ELISA 检测afb1 假阳性 饲料 黄曲霉毒素b1 酶联免疫吸附法 有机相萃取法
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茵陈蒿拮抗AFB_1诱发基因突变和小鼠骨髓细胞遗传损伤的实验研究 被引量:13
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作者 陈少华 洪振丰 +3 位作者 罗仁夏 林丽 刘凌冰 陈紫榕 《癌变.畸变.突变》 CAS CSCD 1998年第1期35-38,共4页
茵陈蒿是常用的保肝中药,具有提高机体免疫功能和抗御肿瘤的作用。本研究利用茵陈蒿可能具有抗突变作用,对强致癌剂AFB1进行Ames试验、小鼠骨髓细胞的遗传毒理学实验,以评价茵陈蒿的抗突变能力。结果表明:1茵陈蒿Ame... 茵陈蒿是常用的保肝中药,具有提高机体免疫功能和抗御肿瘤的作用。本研究利用茵陈蒿可能具有抗突变作用,对强致癌剂AFB1进行Ames试验、小鼠骨髓细胞的遗传毒理学实验,以评价茵陈蒿的抗突变能力。结果表明:1茵陈蒿Ames试验为阴性。在AFB1(5μg/皿)培养基中加入01ml浓度分别为0125、025、05、1、2g/ml的茵陈蒿煎剂,TA98和TA100的回变菌落抑制率达229-5436,差异极其显著(P<001)。2茵陈蒿煎剂对AFB1诱发的小鼠骨髓嗜多染红细胞微核率、染色体畸变率和姊妹染色单体交换率的增高有明显的拮抗作用(P<0.01),且具有明显的剂量———反应关系。本结果提示茵陈蒿可能对癌症预防与治疗有积极的意义。 展开更多
关键词 茵陈蒿 微核 染色体畸变 AMES试验 抗肿瘤作用
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黄曲霉毒素解毒酶催化AFB_1—抗体复合物中抗原变构解离初步研究 被引量:3
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作者 刘大岭 姚冬生 +2 位作者 林伟雄 谢春芳 姚立新 《中国生物工程杂志》 CAS CSCD 2004年第6期93-97,共5页
用免疫学的方法对AFB1在黄曲霉毒素解毒酶催化前后抗原表位发生改变并从抗原抗体复合物中解离的可能性进行研究探讨。结果表明 :经黄曲霉毒素解毒酶处理后产物中有AFB1表位特征产物的量下降了 2 4 8%~ 72 8% ,而灭活酶组平均只下降 2... 用免疫学的方法对AFB1在黄曲霉毒素解毒酶催化前后抗原表位发生改变并从抗原抗体复合物中解离的可能性进行研究探讨。结果表明 :经黄曲霉毒素解毒酶处理后产物中有AFB1表位特征产物的量下降了 2 4 8%~ 72 8% ,而灭活酶组平均只下降 2 0 3% ;活性酶组AFB1的解离率达到 88% ,灭活酶组AFB1的解离率为 8 7% ,两组比较有显著性差异 (P <0 0 1 ) ,而灭活酶组与质控组的比较无显著性差异 (P >0 0 5 )。分子的模拟计算也支持以上结果。 展开更多
关键词 抗原表位 亲和吸附 afbl 黄曲霉毒素 解毒酶
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GSTM1 and XRCC3 Polymorphisms:Effects on Levels of Aflatoxin B1-DNA Adducts 被引量:2
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作者 Xi-dai Long Yun Ma Zhou-lin Deng 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2009年第3期177-184,共8页
Objective: Aflatoxin B1 (AFB1), which can cause the formation of AFB1-DNA adducts, is a known human carcinogen. AFB1-exposure individuals with inherited susceptible carcinogen-metabolizing or repairing genotypes ma... Objective: Aflatoxin B1 (AFB1), which can cause the formation of AFB1-DNA adducts, is a known human carcinogen. AFB1-exposure individuals with inherited susceptible carcinogen-metabolizing or repairing genotypes may experience an increased risk of genotoxicity. This study was designed to investigate whether the polymorphisms of two genes, the metabolic gene Glutathione S-transferase M1 (GSTM1) and DNA repair gene x-ray repair cross-complementing group 3 (XRCC3), can affect the levels of AFB1-DNA adducts in Guangxi Population (n= 966) from an AFB1-exposure area. Methods: AFB1-DNA adducts were measured by ELISA, and GSTM1 and XRCC3 codon 241 genotypes were identified by PCR-RFLP. Results: The GSTM1-null genotype [adjusted odds ratio (OR) = 2.09; 95% confidence interval (CI) = 1.61-2.71] and XRCC3 genotypes with 241 Met alleles [i.e., XRCC3-TM and -MM, adjusted ORs (95% CI) were 1.43 (1.08-1.89) and 2.42 (1.13-5.22), respectively] were significantly associated with higher levels of AFB1-DNA adducts. Compared with those individuals who did not express any putative risk genotypes as reference (OR = 1), individuals featuring all of the putative risk genotypes did experience a significantly higher DNA-adduct levels (adjusted ORs were 2.87 for GSTM1-null and XRCC3-TM; 5.83 for GSTM1-null and XRCC3-MM). Additionally, there was a positive joint effect between XRCC3 genotypes and long-term AFB1 exposure in the formation of AFB1-DNA adducts. Conclusion: These results suggest that individuals with susceptible genotypes GSTM1-null, XRCC3-TM, or XRCC3-MM may experience an increased risk of DNA damage elicited by AFB1 exposure. 展开更多
关键词 aflatoxin b1 (afb1) afb1-DNA adducts GSTM1 XRCC3 POLYMORPHISM
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免疫亲和柱-HPLC在食醋AFB_1含量测定中的应用研究 被引量:2
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作者 孙林超 黄亚东 《中国酿造》 CAS 北大核心 2008年第10X期81-83,共3页
将免疫亲和柱与HPLC相结合,对食醋中AFB1的含量进行分析,建立免疫亲和柱、柱后衍生、高效液相色谱结合荧光检测器测定食醋中AFB1含量的方法。流动相为甲醇-水(65+35),Agilent-Cl8柱(4.6mm×300mm,5μm),流量为0.8mL/min,激发波长为3... 将免疫亲和柱与HPLC相结合,对食醋中AFB1的含量进行分析,建立免疫亲和柱、柱后衍生、高效液相色谱结合荧光检测器测定食醋中AFB1含量的方法。流动相为甲醇-水(65+35),Agilent-Cl8柱(4.6mm×300mm,5μm),流量为0.8mL/min,激发波长为365nm,发射波长为440nm。实验表明:当AFB1的含量在10ng/mL^50ng/mL时,AFB1的浓度与峰面积呈线性关系,加标回收率为89.36%~99.19%,最低检出限为0.1μg/kg,相对标准偏差为1.19%~5.25%。 展开更多
关键词 黄曲霉毒素b1(afb1) 免疫亲和柱 HPLC 柱后衍生 食醋
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有机相萃取法对ELISA检测饲料中AFB_1结果的影响 被引量:2
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作者 柏凡 王宇萍 《中国饲料》 北大核心 2004年第11期36-38,共3页
关键词 有机相萃取法 ELISA 饲料检验 afb1 黄曲霉毒素b1 含量测定
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黄曲霉毒素B_1(AFB_1)降解菌的筛选鉴定 被引量:4
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作者 陈晓飞 周伏忠 +2 位作者 孙玉飞 宁萌 常丽 《河南科学》 2011年第12期1447-1450,共4页
以香豆素为唯一碳源和能源的培养基进行初筛,然后将菌株与黄曲霉毒素B1(AFB1)共培养后测定AFB1降解率的方法进行复筛,最后筛选出4株高活性AFB1降解菌;其中G3-21和T1-4-1对AFB1降解率可达到65%,Y2-30和T1-3-7对AFB1的降解率达到45%以上,... 以香豆素为唯一碳源和能源的培养基进行初筛,然后将菌株与黄曲霉毒素B1(AFB1)共培养后测定AFB1降解率的方法进行复筛,最后筛选出4株高活性AFB1降解菌;其中G3-21和T1-4-1对AFB1降解率可达到65%,Y2-30和T1-3-7对AFB1的降解率达到45%以上,形态和16S rDNA序列分析鉴定结果表明,G3-21和Y2-30为恶臭假单胞菌(Pseudomonas putida),T1-4-1为链霉菌(Streptomyces sp.),T1-3-7为厚壁菌(Firmicutes bacterium). 展开更多
关键词 黄曲霉毒素b1 降解菌 筛选 鉴定
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索诺拉沙漠芽孢杆菌s262降解黄曲霉毒素B1发酵条件优化及活性物质初步分析 被引量:1
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作者 吴甜甜 李晓亚 +3 位作者 刘亚楠 葛文沛 王洪玲 刘娜 《河南工业大学学报(自然科学版)》 CAS 北大核心 2023年第6期83-88,共6页
为探究索诺拉沙漠芽孢杆菌s262中降解黄曲霉毒素B1(Aflatoxin B1,AFB1)的活性物质,在单因素试验基础上,结合响应面法对菌株s262降解AFB1的发酵条件进行了优化,使用硫酸铵沉淀发酵上清液中的蛋白质,对粗酶液进行了质谱分析及酶活检测。... 为探究索诺拉沙漠芽孢杆菌s262中降解黄曲霉毒素B1(Aflatoxin B1,AFB1)的活性物质,在单因素试验基础上,结合响应面法对菌株s262降解AFB1的发酵条件进行了优化,使用硫酸铵沉淀发酵上清液中的蛋白质,对粗酶液进行了质谱分析及酶活检测。结果表明:降解活性物质主要来自菌株的胞外代谢产物;最优发酵条件为种子液接种量6%、发酵时间48 h、发酵温度38℃;质谱检测得出的过氧化氢酶活性与AFB1降解率呈显著正相关(P<0.01)。推测过氧化氢酶为菌株s262发酵液降解AFB1的主要活性物质。 展开更多
关键词 黄曲霉毒素b1 生物脱毒 活性产物 发酵条件
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在HBV和(或)AFB_1诱发树鼩肝癌过程中肝组织ras癌基因的表达 被引量:3
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作者 班克臣 苏建家 +6 位作者 杨春 黄国华 覃柳亮 李瑗 罗小玲 段小娴 严瑞琪 《实验动物科学与管理》 1999年第1期4-7,共4页
为了解在人乙型肝炎病毒(HBV)和/或黄曲霉毒素B1(AFB1)诱发树肝癌过程中P21在肝组织的表达情况,用免疫组化方法观察人HBV+AFB1双因素组(A组)、单因素HBV组(B组)、单因素AFB1组(C组)、及对... 为了解在人乙型肝炎病毒(HBV)和/或黄曲霉毒素B1(AFB1)诱发树肝癌过程中P21在肝组织的表达情况,用免疫组化方法观察人HBV+AFB1双因素组(A组)、单因素HBV组(B组)、单因素AFB1组(C组)、及对照组(D组)实验第44周、105周、119周动物肝活检组织P21蛋白的表达情况。结果,至119周,累计肝组织P21阳性动物百分率分别为353、53、133、0。表明人HBV与AFB1可协同促进树肝组织P21的表达。 展开更多
关键词 树QU 肝癌 乙型肝炎病毒 黄曲霉毒素b1
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耐盐酵母菌Candida versatilis去除AFB_1的机制探究
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作者 何菲 李从虎 +2 位作者 崔瑞迎 吴重德 周荣清 《中国调味品》 CAS 北大核心 2014年第4期8-12,20,共6页
完成了10株应用于酱油和豆酱等调味品生产中的酵母菌和乳酸菌在含有10ng/mL AFB1液体培养基中除去AFB1能力的评估。结果表明:所有的菌株均能去除AFB1,去除效率因菌株而异。Candida versatilis CGMCC 3790去除率较高。优化所得的最适工... 完成了10株应用于酱油和豆酱等调味品生产中的酵母菌和乳酸菌在含有10ng/mL AFB1液体培养基中除去AFB1能力的评估。结果表明:所有的菌株均能去除AFB1,去除效率因菌株而异。Candida versatilis CGMCC 3790去除率较高。优化所得的最适工艺参数:温度为30℃,起始pH为5.0,接种量为15%,时间为120h。评估培养物上清、胞内组分、菌体细胞状态去除AFB1的能力和AFB1浓度对去除率影响的结果表明,其去除机制可能类似非耐盐酵母菌,是细胞壁吸附所致。 展开更多
关键词 耐盐酵母菌 afb1 去除机制 酶联免疫法 aflatoxin b1
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