Terahertz metamaterial biosensors have attracted significant attention in the biological field due to their advantages of label-free,real-time and in situ detection.In this paper,a highly sensitive metamaterial sensor...Terahertz metamaterial biosensors have attracted significant attention in the biological field due to their advantages of label-free,real-time and in situ detection.In this paper,a highly sensitive metamaterial sensor with semi-ring mirror symmetry based on toroidal dipole resonance is designed for a new metamaterial biosensor.It is shown that a refractive index sensitivity of 337.5 GHz per refractive index unit can be achieved under an analyte of saturated thickness near a 1.33 THz transmission dip.For biosensor samples where aflatoxin B1 is dropped on the metamaterial surface in our experiment,dip amplitudes of transmission varying from 0.1904 to 0.203 and 0.2093 are observed as aflatoxin B1 concentrations are altered from 0 to 0.001μg·ml-1 and to 0.01μg·ml-1,respectively.Furthermore,when aflatoxin B1 concentrations are 0.1μg·ml-1,1μg·ml-1,10μg·ml-1 and 100μg·ml-1,dip amplitudes of 0.2179,0.226,0.2384 and 0.2527 and dip redshifts of 10.1 GHz,20.1 GHz,27.7 GHz and 37.6 GHz are respectively observed.These results illustrate high-sensitivity,label-free detection of aflatoxin B1,enriching the applications of sensors in the terahertz domain.展开更多
BACKGROUND The dysregulation of tissue inhibitor of metalloproteinase-3(TIMP3)was positively correlated with the progression of hepatocellular carcinoma(HCC).However,it is not clear whether TIMP3 expression is associa...BACKGROUND The dysregulation of tissue inhibitor of metalloproteinase-3(TIMP3)was positively correlated with the progression of hepatocellular carcinoma(HCC).However,it is not clear whether TIMP3 expression is associated with the clinico-pathological features and prognosis of aflatoxin B1(AFB1)-related HCC(AHCC).A retrospective study,including 182 patients with AHCC,was conducted to explore the link between TIMP3 expression in cancerous tissues and the clinico-pathological characteristics and prognosis of AHCC.TIMP3 expression was detected by immunohistochemistry and its effects on the clinicopathological features and prognosis of AHCC were evaluated by Kaplan-Meier survival analysis and Cox regression survival analysis.Odds ratio,hazard ratio(HR),median overall survival time(MST),median tumor recurrence-free survival time(MRT),and corresponding 95%confidential interval(CI)was calculated to RESULTS Kaplan-Meier survival analysis showed that compared with high TIMP3 expression,low TIMP3 expression in tumor tissues significantly decreased the MST(36.00 mo vs 18.00 mo)and MRT(32.00 mo vs 16 mo)of patients with AHCC.Multivariate Cox regression survival analysis further proved that decreased expression of TIMP3 increased the risk of death(HR=2.85,95%CI:2.04-4.00)and tumor recurrence(HR=2.26,95%CI:1.57-3.26).Furthermore,decreased expression of TIMP3 protein in tissues with AHCC was significantly correlated with tumor clinicopatho-logical features,such as tumor size,tumor grade and stage,tumor microvessel density,and tumor blood invasion.Additionally,TIMP3 protein expression was also negatively associated with amount of AFB1-DNA adducts in tumor tissues.CONCLUSION These findings indicate that the dysregulation of TIMP3 expression is related to AHCC biological behaviors and affects tumor outcome,suggesting that TIMP3 may act as a prognostic biomarker for AHCC.展开更多
San-Huang chicken is a high-quality breed in China with yellow feather, claw and break. However, the abnormal phenomenon of the yellow shank turning into green shank of San-Huang chicken has been a concern, as it seri...San-Huang chicken is a high-quality breed in China with yellow feather, claw and break. However, the abnormal phenomenon of the yellow shank turning into green shank of San-Huang chicken has been a concern, as it seriously reduces the carcass quality and economic benefit of yellow-feathered broilers. In this study, the cause of this abnormal green skin in shank was systematically investigated. Physiological anatomy revealed that the abnormal skin in shank was primarily due to the deposition of melanin under the dermis. After analyzing multiple potential causes such as heredity(pedigree and genetic markers), environment(water quality monitoring) and feed composition(mycotoxin detection), excessive aflatoxin B1(AFB1) in feed was screened, accompanied with a higher L-dihydroxy-phenylalanine(L-DOPA)(P<0.05) and melanin content(P<0.01). So it was speculated that excessive AFB1 might be the main cause of abnormal green skin in shank. Subsequently, the further results showed that a high concentration of AFB1(>170 μg kg–1)indeed induced the abnormal green skin in shank compared to the normal AFB1 content(<10 μg kg–1), and the mRNA levels of TYR, TYRP1, MITE, MC1R and EDN3 genes related to melanin deposition would significantly up-regulate(P<0.01) and the content and activity of tyrosinase(TyR) significantly increased(P<0.05). At the same time, the content of L-DOPA and melanin deposition also increased significantly(P<0.01), which also confirmed the effect of excessive AFB1 on melanin deposition in skin of shank. Results of additional experiments revealed that the AFB1's negative effect on melanin deposition in skin of shank could last for a longer time. Taken together, the results of this study explained the occurrence and possible mechanisms of the abnormal AFB1-related green skin in shank of chickens. Excessive AFB1 in diets increased the L-DOPA content and melanin abnormal deposition in the chicken shank possibly via promoting TyR content and activity, and the expression of melanin synthesis-related genes. Furthermore, our findings once again raised the alarm of the danger of AFB1 in the broiler production.展开更多
Purpose: Aflatoxin B<sub>1</sub> is the most common mycotoxin in cereal crops;it is of stronger toxicity and has a carcinogenic effect. In recent years, a series of fluorescence sensors constructed on the ...Purpose: Aflatoxin B<sub>1</sub> is the most common mycotoxin in cereal crops;it is of stronger toxicity and has a carcinogenic effect. In recent years, a series of fluorescence sensors constructed on the basis of MoS<sub>2</sub>NS fluorescence quenching property have become a research hotspot. Therefore, we can construct a fast and simple analysis method with high specificity to detect AFB<sub>1</sub> by utilizing MoS<sub>2</sub>NS, which can be effectively applied to food safety monitoring and clinical diagnosis. Method: In the current research, a fluorescence biosensor is developed on the basis of a new type of two-dimensional nano-material namely MoS<sub>2</sub>NS applied for the detection of AFB<sub>1</sub>. The fluorescence of Apt@AFB<sub>1</sub> can be quickly quenched by MoS<sub>2</sub>NS through the fluorescence resonance energy transfer (FRET). When the target molecule AFB<sub>1</sub> exists, after the specificity binding between AFB<sub>1</sub> and aptamer, the Apt@AFB<sub>1</sub> loses its single stranded structure and is away from MoS<sub>2</sub>NS, and the fluorescence of Apt®AFB<sub>1</sub> cannot be quenched effectively. Such sensing signals can be used to achieve the sensitive detection of AFB<sub>1</sub>. Result: With this new method, under the optimized conditions, the AFB<sub>1</sub> is analyzed in the MoS<sub>2</sub>NS/Apt®AFB<sub>1</sub> sensing platform. Within the dynamic range of 0.2 - 25 ng/mL, the sensing platform expresses a good linear response to the level of AFB<sub>1</sub> with the R<sup>2</sup> = 0.9964 and LOD as 90 pg/mL. This method is applied to detect the actual serum samples and soybean milk with the recovery rate of 93.10% - 107.23% and 95.15% - 102.60% separately, and it can be used in the quantitative detection under the interference of other mycotoxins in a relatively accurate way. Conclusion: It is proved that this new detection method can be used as a potential biosensor platform for the detection of AFB<sub>1</sub>. This detection method features several advantages such as specificity, rapidness and low costs, which can meet the requirement of trace detection in clinical detection and food safety.展开更多
Objective： Aflatoxin B1 （AFB1）, which can cause the formation of AFB1-DNA adducts, is a known human carcinogen. AFB1-exposure individuals with inherited susceptible carcinogen-metabolizing or repairing genotypes ma...Objective： Aflatoxin B1 （AFB1）, which can cause the formation of AFB1-DNA adducts, is a known human carcinogen. AFB1-exposure individuals with inherited susceptible carcinogen-metabolizing or repairing genotypes may experience an increased risk of genotoxicity. This study was designed to investigate whether the polymorphisms of two genes, the metabolic gene Glutathione S-transferase M1 （GSTM1） and DNA repair gene x-ray repair cross-complementing group 3 （XRCC3）, can affect the levels of AFB1-DNA adducts in Guangxi Population （n= 966） from an AFB1-exposure area. Methods： AFB1-DNA adducts were measured by ELISA, and GSTM1 and XRCC3 codon 241 genotypes were identified by PCR-RFLP. Results： The GSTM1-null genotype [adjusted odds ratio （OR） = 2.09; 95% confidence interval （CI） = 1.61-2.71] and XRCC3 genotypes with 241 Met alleles [i.e., XRCC3-TM and -MM, adjusted ORs （95% CI） were 1.43 （1.08-1.89） and 2.42 （1.13-5.22）, respectively] were significantly associated with higher levels of AFB1-DNA adducts. Compared with those individuals who did not express any putative risk genotypes as reference （OR = 1）, individuals featuring all of the putative risk genotypes did experience a significantly higher DNA-adduct levels （adjusted ORs were 2.87 for GSTM1-null and XRCC3-TM; 5.83 for GSTM1-null and XRCC3-MM）. Additionally, there was a positive joint effect between XRCC3 genotypes and long-term AFB1 exposure in the formation of AFB1-DNA adducts. Conclusion： These results suggest that individuals with susceptible genotypes GSTM1-null, XRCC3-TM, or XRCC3-MM may experience an increased risk of DNA damage elicited by AFB1 exposure.展开更多
基金Project supported by the National Natural Science Foundation of China(Grant Nos.61927813,61865009,and 12104203)Jiangxi Provincial Natural Science Foundation(Grant No.20212ACB201007).
文摘Terahertz metamaterial biosensors have attracted significant attention in the biological field due to their advantages of label-free,real-time and in situ detection.In this paper,a highly sensitive metamaterial sensor with semi-ring mirror symmetry based on toroidal dipole resonance is designed for a new metamaterial biosensor.It is shown that a refractive index sensitivity of 337.5 GHz per refractive index unit can be achieved under an analyte of saturated thickness near a 1.33 THz transmission dip.For biosensor samples where aflatoxin B1 is dropped on the metamaterial surface in our experiment,dip amplitudes of transmission varying from 0.1904 to 0.203 and 0.2093 are observed as aflatoxin B1 concentrations are altered from 0 to 0.001μg·ml-1 and to 0.01μg·ml-1,respectively.Furthermore,when aflatoxin B1 concentrations are 0.1μg·ml-1,1μg·ml-1,10μg·ml-1 and 100μg·ml-1,dip amplitudes of 0.2179,0.226,0.2384 and 0.2527 and dip redshifts of 10.1 GHz,20.1 GHz,27.7 GHz and 37.6 GHz are respectively observed.These results illustrate high-sensitivity,label-free detection of aflatoxin B1,enriching the applications of sensors in the terahertz domain.
基金the Science-Technology Planning Project of Guangxi,No.Guike-AD19245174Guangxi Training Program for Medical High-level Academic Leaders,No.6 of Guiweikejiaofa[2020]-15+3 种基金Bose Talent Highland,No.2020-3-2Building Projects from the Key Laboratory of Molecular Pathology(Hepatobiliary Diseases)of Guangxi,No.Guiweikejiaofa[2020]-17the Key Laboratory of Tumor Molecular Pathology of Guangxi Colleges and Universities,No.Guijiaokeyan[2022]-10and Clinical Key Specialty Building Project(For Pathology)of Guangxi,No.Guiweiyifa[2022]-21.
文摘BACKGROUND The dysregulation of tissue inhibitor of metalloproteinase-3(TIMP3)was positively correlated with the progression of hepatocellular carcinoma(HCC).However,it is not clear whether TIMP3 expression is associated with the clinico-pathological features and prognosis of aflatoxin B1(AFB1)-related HCC(AHCC).A retrospective study,including 182 patients with AHCC,was conducted to explore the link between TIMP3 expression in cancerous tissues and the clinico-pathological characteristics and prognosis of AHCC.TIMP3 expression was detected by immunohistochemistry and its effects on the clinicopathological features and prognosis of AHCC were evaluated by Kaplan-Meier survival analysis and Cox regression survival analysis.Odds ratio,hazard ratio(HR),median overall survival time(MST),median tumor recurrence-free survival time(MRT),and corresponding 95%confidential interval(CI)was calculated to RESULTS Kaplan-Meier survival analysis showed that compared with high TIMP3 expression,low TIMP3 expression in tumor tissues significantly decreased the MST(36.00 mo vs 18.00 mo)and MRT(32.00 mo vs 16 mo)of patients with AHCC.Multivariate Cox regression survival analysis further proved that decreased expression of TIMP3 increased the risk of death(HR=2.85,95%CI:2.04-4.00)and tumor recurrence(HR=2.26,95%CI:1.57-3.26).Furthermore,decreased expression of TIMP3 protein in tissues with AHCC was significantly correlated with tumor clinicopatho-logical features,such as tumor size,tumor grade and stage,tumor microvessel density,and tumor blood invasion.Additionally,TIMP3 protein expression was also negatively associated with amount of AFB1-DNA adducts in tumor tissues.CONCLUSION These findings indicate that the dysregulation of TIMP3 expression is related to AHCC biological behaviors and affects tumor outcome,suggesting that TIMP3 may act as a prognostic biomarker for AHCC.
基金funded by the grants from the China Agriculture Research System of MOF and MARA (CARS-41)the Agricultural Science and Technology Innovation Program, China (ASTIP-IAS04)。
文摘San-Huang chicken is a high-quality breed in China with yellow feather, claw and break. However, the abnormal phenomenon of the yellow shank turning into green shank of San-Huang chicken has been a concern, as it seriously reduces the carcass quality and economic benefit of yellow-feathered broilers. In this study, the cause of this abnormal green skin in shank was systematically investigated. Physiological anatomy revealed that the abnormal skin in shank was primarily due to the deposition of melanin under the dermis. After analyzing multiple potential causes such as heredity(pedigree and genetic markers), environment(water quality monitoring) and feed composition(mycotoxin detection), excessive aflatoxin B1(AFB1) in feed was screened, accompanied with a higher L-dihydroxy-phenylalanine(L-DOPA)(P<0.05) and melanin content(P<0.01). So it was speculated that excessive AFB1 might be the main cause of abnormal green skin in shank. Subsequently, the further results showed that a high concentration of AFB1(>170 μg kg–1)indeed induced the abnormal green skin in shank compared to the normal AFB1 content(<10 μg kg–1), and the mRNA levels of TYR, TYRP1, MITE, MC1R and EDN3 genes related to melanin deposition would significantly up-regulate(P<0.01) and the content and activity of tyrosinase(TyR) significantly increased(P<0.05). At the same time, the content of L-DOPA and melanin deposition also increased significantly(P<0.01), which also confirmed the effect of excessive AFB1 on melanin deposition in skin of shank. Results of additional experiments revealed that the AFB1's negative effect on melanin deposition in skin of shank could last for a longer time. Taken together, the results of this study explained the occurrence and possible mechanisms of the abnormal AFB1-related green skin in shank of chickens. Excessive AFB1 in diets increased the L-DOPA content and melanin abnormal deposition in the chicken shank possibly via promoting TyR content and activity, and the expression of melanin synthesis-related genes. Furthermore, our findings once again raised the alarm of the danger of AFB1 in the broiler production.
文摘Purpose: Aflatoxin B<sub>1</sub> is the most common mycotoxin in cereal crops;it is of stronger toxicity and has a carcinogenic effect. In recent years, a series of fluorescence sensors constructed on the basis of MoS<sub>2</sub>NS fluorescence quenching property have become a research hotspot. Therefore, we can construct a fast and simple analysis method with high specificity to detect AFB<sub>1</sub> by utilizing MoS<sub>2</sub>NS, which can be effectively applied to food safety monitoring and clinical diagnosis. Method: In the current research, a fluorescence biosensor is developed on the basis of a new type of two-dimensional nano-material namely MoS<sub>2</sub>NS applied for the detection of AFB<sub>1</sub>. The fluorescence of Apt@AFB<sub>1</sub> can be quickly quenched by MoS<sub>2</sub>NS through the fluorescence resonance energy transfer (FRET). When the target molecule AFB<sub>1</sub> exists, after the specificity binding between AFB<sub>1</sub> and aptamer, the Apt@AFB<sub>1</sub> loses its single stranded structure and is away from MoS<sub>2</sub>NS, and the fluorescence of Apt®AFB<sub>1</sub> cannot be quenched effectively. Such sensing signals can be used to achieve the sensitive detection of AFB<sub>1</sub>. Result: With this new method, under the optimized conditions, the AFB<sub>1</sub> is analyzed in the MoS<sub>2</sub>NS/Apt®AFB<sub>1</sub> sensing platform. Within the dynamic range of 0.2 - 25 ng/mL, the sensing platform expresses a good linear response to the level of AFB<sub>1</sub> with the R<sup>2</sup> = 0.9964 and LOD as 90 pg/mL. This method is applied to detect the actual serum samples and soybean milk with the recovery rate of 93.10% - 107.23% and 95.15% - 102.60% separately, and it can be used in the quantitative detection under the interference of other mycotoxins in a relatively accurate way. Conclusion: It is proved that this new detection method can be used as a potential biosensor platform for the detection of AFB<sub>1</sub>. This detection method features several advantages such as specificity, rapidness and low costs, which can meet the requirement of trace detection in clinical detection and food safety.
基金supported by the National Natural Science Foundation of China (No.39860032)the Youth Science Foundation of Guangxi (No.0833097)
文摘Objective： Aflatoxin B1 （AFB1）, which can cause the formation of AFB1-DNA adducts, is a known human carcinogen. AFB1-exposure individuals with inherited susceptible carcinogen-metabolizing or repairing genotypes may experience an increased risk of genotoxicity. This study was designed to investigate whether the polymorphisms of two genes, the metabolic gene Glutathione S-transferase M1 （GSTM1） and DNA repair gene x-ray repair cross-complementing group 3 （XRCC3）, can affect the levels of AFB1-DNA adducts in Guangxi Population （n= 966） from an AFB1-exposure area. Methods： AFB1-DNA adducts were measured by ELISA, and GSTM1 and XRCC3 codon 241 genotypes were identified by PCR-RFLP. Results： The GSTM1-null genotype [adjusted odds ratio （OR） = 2.09; 95% confidence interval （CI） = 1.61-2.71] and XRCC3 genotypes with 241 Met alleles [i.e., XRCC3-TM and -MM, adjusted ORs （95% CI） were 1.43 （1.08-1.89） and 2.42 （1.13-5.22）, respectively] were significantly associated with higher levels of AFB1-DNA adducts. Compared with those individuals who did not express any putative risk genotypes as reference （OR = 1）, individuals featuring all of the putative risk genotypes did experience a significantly higher DNA-adduct levels （adjusted ORs were 2.87 for GSTM1-null and XRCC3-TM; 5.83 for GSTM1-null and XRCC3-MM）. Additionally, there was a positive joint effect between XRCC3 genotypes and long-term AFB1 exposure in the formation of AFB1-DNA adducts. Conclusion： These results suggest that individuals with susceptible genotypes GSTM1-null, XRCC3-TM, or XRCC3-MM may experience an increased risk of DNA damage elicited by AFB1 exposure.
