Ni^(2+)and Cd^(2+)in wastewater accumulated through the ecological chain and could jeopardize human health.Adsorption of Ni^(2+)and Cd^(2+)from wastewater using recovered perlite was an important way to solve the prob...Ni^(2+)and Cd^(2+)in wastewater accumulated through the ecological chain and could jeopardize human health.Adsorption of Ni^(2+)and Cd^(2+)from wastewater using recovered perlite was an important way to solve the problem of resource utilization of solid waste from agar production.Our previous study confirmed that recovered perlite from agar extraction residue had better pore size and specific surface area than commercial perlite.However,the adsorption efficiency and adsorption mechanism of recovered perlite were the main factors limiting its adsorption application.The adsorption process of Ni^(2+)and Cd^(2+)by recovered perlite in aqueous solution was described by the pseudo-second-order kinetic equation,and the relevant adsorption mechanism was mainly chemisorption.Compared with commercial perlite,the adsorption removal rate of Ni^(2+)and Cd^(2+)by enzymatic recovered perlite could reach 92.9%and 89.2%,respectively,and were improved by 12.63%and 13.03%.Langmuir isothermal adsorption model could better describe the isothermal adsorption process of recovered perlite on heavy metal Ni^(2+)and Cd^(2+),and the relevant adsorption mechanism was mainly monolayer adsorption.The X-ray photoelectron spectroscopy(XPS)results indicated that the decrease of Si—O Si^(2+)hydroxyl coordination bond and the increase of C—Si bond might make the binding effect of recovered perlite with heavy metals stronger.The competitive adsorption of Ni^(2+)and Cd^(2+)by recovered perlite was still dominated by chemisorption and monolayer adsorption.This study was expected to provide a theoretical basis and technical support for the removal of Ni^(2+)and Cd^(2+)from wastewater using recovered perlite from seaweed residue.展开更多
The purpose of this study was to identify and compare the degradation efficiencies of free and entrapped bacterial consortia(Staphylococcus capitis CP053957.1 and Achromobacter marplatensis MT078618.1)to different pol...The purpose of this study was to identify and compare the degradation efficiencies of free and entrapped bacterial consortia(Staphylococcus capitis CP053957.1 and Achromobacter marplatensis MT078618.1)to different polymers such as Sodium Alginate(SA),Sodium Alginate/Poly(Vinyl Alcohol)(SA/PVA),and Bushnell Haas Agar(BHA).In addition to SA and SA/PVA,which are cost-effective,non-toxic and have different functional groups,BHA,which is frequently encountered in laboratory-scale studies but has not been used as an entrapment material until now.Based on these,the polymers with different surface morphologies and chemical compositions were analyzed by SEM and FT-IR.While the petroleum removal efficiency was higher with the entrapped bacterial consortia than with the free one,BHA-entrapped bacterial consortium enhanced the petroleum removal more than SA and SA/PVA.Accordingly,the degradation rate of bacterial consortia entrapped with BHA was 2.039 day^(-1),SA/PVA was 1.560,SA was 0.993,the half-life period of BHA-entrapped bacterial consortia is quite low(t_(1/2)=0.339)compared with SA(t_(1/2)=0.444)and SA/PVA(t_(1/2)=0.697).The effects of the four main factors such as:amount of BHA(0.5,1,1.5,2,2.5,3 g),disc size(4,5,6,7,8 mm),inoculum concentration(1,2.5,5,7.5,10 mL),and incubation period on petroleum removal were also investigated.The maximum petroleum removal(94.5%)was obtained at≥2.5 mL of bacterial consortium entrapped in 2 g BHA with a 7 mm disc size at 168 h and the results were also confirmed by statistical analysis.Although a decrease was observed during the reuse of bacterial consortium entrapped in BHA,the petroleum removal was still above 50%at 10th cycle.Based on GC-MS analysis,the removal capacity of BHA-entrapped consortium was over 90%for short-chain n-alkanes and 80%for medium-chain n-alkanes.Overall,the obtained data are expected to provide a potential guideline in cleaning up the large-scale oil pollution in the future.Since there has been no similar study investigating petroleum removal with the bacterial consortia entrapped with BHA,this novel entrapment material can potentially be used in the treatment of petroleum pollution in advanced remediation studies.展开更多
Three materials(agar,konjac glucomannan(KGM)andκ-carrageenan)were used to prepare ternary systems,i.e.,sol-gels and their dried composites conditioned at varied relative humidity(RH)(33%,54%and 75%).Combined methods,...Three materials(agar,konjac glucomannan(KGM)andκ-carrageenan)were used to prepare ternary systems,i.e.,sol-gels and their dried composites conditioned at varied relative humidity(RH)(33%,54%and 75%).Combined methods,e.g.,scanning electron microscopy,small-angle X-ray scattering,infrared spectroscopy(IR)and X-ray diffraction(XRD),were used to disclose howκ-carrageenan addition tailors the features of agar/KGM/κ-carrageenan ternary system.As affirmed by IR and XRD,the ternary systems withκ-carrageenan below 25%(agar/KGM/carrageenan,50:25:25,m/m)displayed proper component interactions,which increased the sol-gel transition temperature and the hardness of obtained gels.For instance,the ternary composites could show hardness about 3 to 4 times higher than that for binary counterpart.These gels were dehydrated to acquire ternary composites.Compared to agar/KGM composite,the ternary composites showed fewer crystallites and nanoscale orders,and newly-formed nanoscale structures from chain assembly.Such multi-scale structures,for composites withκ-carrageenan below 25%,showed weaker changes with RH,as revealed by especially morphologic and crystalline features.Consequently,the ternary composites with lessκ-carrageenan(below 25%)exhibited stabilized elongation at break and hydrophilicity at different RHs.This hints to us that agar/KGM/κ-carrageenan composite systems can display series applications with improved features,e.g.,increased sol-gel transition point.展开更多
The sulfate groups in agar structure played a good role in the formation of fiber.However,commercially available agar is usually extracted from red algae by alkali treatment to decrease the content of sulfate group fo...The sulfate groups in agar structure played a good role in the formation of fiber.However,commercially available agar is usually extracted from red algae by alkali treatment to decrease the content of sulfate group for the purpose of high gel strength.In this paper,an alkali-free method of agar extraction from Gracilaria lemaneiformis was proposed for the wet-spinning purpose.This method is environmentally friendly,reduces the extraction steps,saves energy,and reduces the production cost of agar fiber.The improved agar preparation process not only has higher agar yield,but also has higher molecular weight and sulfate group content,which is beneficial to the preparation and forming of fiber and makes the fiber have higher mechanical strength and elongation.Therefore,this extraction technology has broad application prospect in the textile field.展开更多
【目的】建立并优化布鲁氏菌天然半抗原琼脂扩散试验(Native hapten agar gel immuno-diffusion test,NH-AGID),检测临床样品。【方法】建立NH-AGID方法,评价方法特异性、敏感性及重复性;检测免疫地区2287份、非免疫地区252份牛、羊血...【目的】建立并优化布鲁氏菌天然半抗原琼脂扩散试验(Native hapten agar gel immuno-diffusion test,NH-AGID),检测临床样品。【方法】建立NH-AGID方法,评价方法特异性、敏感性及重复性;检测免疫地区2287份、非免疫地区252份牛、羊血清。【结果】建立了NH-AGID方法,检测稳定,重复性好,具有较高的特异性;对自然感染阳性动物检测敏感性低于80%,对排菌动物的检测敏感性高于90%,检出布病阳性抗体的阈值高于RBT。免疫地区血清NH抗体平均检出率17.8%,LPS抗体平均检出率52.3%;非免疫地区血清NH抗体平均检出率40.9%,LPS抗体平均检出率54.8%。【结论】应用NH-AGID方法可鉴别区分免疫地区牛、羊布病感染动物和免疫动物。检出NH抗体动物处于布鲁氏菌活动期或排菌期为布病感染动物,应及时剔除出群。展开更多
[Objective] This study aimed to compare the detection results of antibodies against infectious bursal disease virus with test strips and agar gel immunodiffusion method. [Method] Antibodies against infectious bursal d...[Objective] This study aimed to compare the detection results of antibodies against infectious bursal disease virus with test strips and agar gel immunodiffusion method. [Method] Antibodies against infectious bursal disease virus in chicken serum were detected using test strips developed in our laboratory, and the results were comparad^with that using traditional agar diffusion method. [Result] The comparative study of the two methods showed that the sensitivity of test strips was eight times over agar gel immunodiffusion; test strips showed higher detection rate in the deter- mination test of 216 clinical samples, with high specificity, easy preservation, and simple and rapid operation, thereby being more suitable for the monitoring of clinical antibodies. [Conclusion] Test strips could replace the existing serological methods, having great promotion and application value in antibody monitoring.展开更多
基金financially supported by National Natural Science Foundation of China(22038012,32172339,and 22178142)National Key Research and Development Program(2023YF D2100603)。
文摘Ni^(2+)and Cd^(2+)in wastewater accumulated through the ecological chain and could jeopardize human health.Adsorption of Ni^(2+)and Cd^(2+)from wastewater using recovered perlite was an important way to solve the problem of resource utilization of solid waste from agar production.Our previous study confirmed that recovered perlite from agar extraction residue had better pore size and specific surface area than commercial perlite.However,the adsorption efficiency and adsorption mechanism of recovered perlite were the main factors limiting its adsorption application.The adsorption process of Ni^(2+)and Cd^(2+)by recovered perlite in aqueous solution was described by the pseudo-second-order kinetic equation,and the relevant adsorption mechanism was mainly chemisorption.Compared with commercial perlite,the adsorption removal rate of Ni^(2+)and Cd^(2+)by enzymatic recovered perlite could reach 92.9%and 89.2%,respectively,and were improved by 12.63%and 13.03%.Langmuir isothermal adsorption model could better describe the isothermal adsorption process of recovered perlite on heavy metal Ni^(2+)and Cd^(2+),and the relevant adsorption mechanism was mainly monolayer adsorption.The X-ray photoelectron spectroscopy(XPS)results indicated that the decrease of Si—O Si^(2+)hydroxyl coordination bond and the increase of C—Si bond might make the binding effect of recovered perlite with heavy metals stronger.The competitive adsorption of Ni^(2+)and Cd^(2+)by recovered perlite was still dominated by chemisorption and monolayer adsorption.This study was expected to provide a theoretical basis and technical support for the removal of Ni^(2+)and Cd^(2+)from wastewater using recovered perlite from seaweed residue.
文摘The purpose of this study was to identify and compare the degradation efficiencies of free and entrapped bacterial consortia(Staphylococcus capitis CP053957.1 and Achromobacter marplatensis MT078618.1)to different polymers such as Sodium Alginate(SA),Sodium Alginate/Poly(Vinyl Alcohol)(SA/PVA),and Bushnell Haas Agar(BHA).In addition to SA and SA/PVA,which are cost-effective,non-toxic and have different functional groups,BHA,which is frequently encountered in laboratory-scale studies but has not been used as an entrapment material until now.Based on these,the polymers with different surface morphologies and chemical compositions were analyzed by SEM and FT-IR.While the petroleum removal efficiency was higher with the entrapped bacterial consortia than with the free one,BHA-entrapped bacterial consortium enhanced the petroleum removal more than SA and SA/PVA.Accordingly,the degradation rate of bacterial consortia entrapped with BHA was 2.039 day^(-1),SA/PVA was 1.560,SA was 0.993,the half-life period of BHA-entrapped bacterial consortia is quite low(t_(1/2)=0.339)compared with SA(t_(1/2)=0.444)and SA/PVA(t_(1/2)=0.697).The effects of the four main factors such as:amount of BHA(0.5,1,1.5,2,2.5,3 g),disc size(4,5,6,7,8 mm),inoculum concentration(1,2.5,5,7.5,10 mL),and incubation period on petroleum removal were also investigated.The maximum petroleum removal(94.5%)was obtained at≥2.5 mL of bacterial consortium entrapped in 2 g BHA with a 7 mm disc size at 168 h and the results were also confirmed by statistical analysis.Although a decrease was observed during the reuse of bacterial consortium entrapped in BHA,the petroleum removal was still above 50%at 10th cycle.Based on GC-MS analysis,the removal capacity of BHA-entrapped consortium was over 90%for short-chain n-alkanes and 80%for medium-chain n-alkanes.Overall,the obtained data are expected to provide a potential guideline in cleaning up the large-scale oil pollution in the future.Since there has been no similar study investigating petroleum removal with the bacterial consortia entrapped with BHA,this novel entrapment material can potentially be used in the treatment of petroleum pollution in advanced remediation studies.
基金the National Natural Science Foundation of China(32172240)BL19U2 beamline of National Facility for Protein Science in Shanghai(NFPS)at Shanghai Synchrotron Radiation Facility,for their assistance during data collection。
文摘Three materials(agar,konjac glucomannan(KGM)andκ-carrageenan)were used to prepare ternary systems,i.e.,sol-gels and their dried composites conditioned at varied relative humidity(RH)(33%,54%and 75%).Combined methods,e.g.,scanning electron microscopy,small-angle X-ray scattering,infrared spectroscopy(IR)and X-ray diffraction(XRD),were used to disclose howκ-carrageenan addition tailors the features of agar/KGM/κ-carrageenan ternary system.As affirmed by IR and XRD,the ternary systems withκ-carrageenan below 25%(agar/KGM/carrageenan,50:25:25,m/m)displayed proper component interactions,which increased the sol-gel transition temperature and the hardness of obtained gels.For instance,the ternary composites could show hardness about 3 to 4 times higher than that for binary counterpart.These gels were dehydrated to acquire ternary composites.Compared to agar/KGM composite,the ternary composites showed fewer crystallites and nanoscale orders,and newly-formed nanoscale structures from chain assembly.Such multi-scale structures,for composites withκ-carrageenan below 25%,showed weaker changes with RH,as revealed by especially morphologic and crystalline features.Consequently,the ternary composites with lessκ-carrageenan(below 25%)exhibited stabilized elongation at break and hydrophilicity at different RHs.This hints to us that agar/KGM/κ-carrageenan composite systems can display series applications with improved features,e.g.,increased sol-gel transition point.
基金supported by the Program of National Natural Science Foundation of China(52173037)Natural Science Foundation of Shandong Province(ZR2020ME061)+2 种基金Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China(IRT14R30)State Key Laboratory of Bio-Fibers and Eco-Textiles of Qingdao University(ZFT201810,ZKT17)Yunnan Expert Workstation Project(202105AF150061).
文摘The sulfate groups in agar structure played a good role in the formation of fiber.However,commercially available agar is usually extracted from red algae by alkali treatment to decrease the content of sulfate group for the purpose of high gel strength.In this paper,an alkali-free method of agar extraction from Gracilaria lemaneiformis was proposed for the wet-spinning purpose.This method is environmentally friendly,reduces the extraction steps,saves energy,and reduces the production cost of agar fiber.The improved agar preparation process not only has higher agar yield,but also has higher molecular weight and sulfate group content,which is beneficial to the preparation and forming of fiber and makes the fiber have higher mechanical strength and elongation.Therefore,this extraction technology has broad application prospect in the textile field.
文摘【目的】建立并优化布鲁氏菌天然半抗原琼脂扩散试验(Native hapten agar gel immuno-diffusion test,NH-AGID),检测临床样品。【方法】建立NH-AGID方法,评价方法特异性、敏感性及重复性;检测免疫地区2287份、非免疫地区252份牛、羊血清。【结果】建立了NH-AGID方法,检测稳定,重复性好,具有较高的特异性;对自然感染阳性动物检测敏感性低于80%,对排菌动物的检测敏感性高于90%,检出布病阳性抗体的阈值高于RBT。免疫地区血清NH抗体平均检出率17.8%,LPS抗体平均检出率52.3%;非免疫地区血清NH抗体平均检出率40.9%,LPS抗体平均检出率54.8%。【结论】应用NH-AGID方法可鉴别区分免疫地区牛、羊布病感染动物和免疫动物。检出NH抗体动物处于布鲁氏菌活动期或排菌期为布病感染动物,应及时剔除出群。
文摘[Objective] This study aimed to compare the detection results of antibodies against infectious bursal disease virus with test strips and agar gel immunodiffusion method. [Method] Antibodies against infectious bursal disease virus in chicken serum were detected using test strips developed in our laboratory, and the results were comparad^with that using traditional agar diffusion method. [Result] The comparative study of the two methods showed that the sensitivity of test strips was eight times over agar gel immunodiffusion; test strips showed higher detection rate in the deter- mination test of 216 clinical samples, with high specificity, easy preservation, and simple and rapid operation, thereby being more suitable for the monitoring of clinical antibodies. [Conclusion] Test strips could replace the existing serological methods, having great promotion and application value in antibody monitoring.
