Maillard reaction(MR)is a non-enzymatic browning reaction commonly seen in food processing,which occurs between reducing sugars and compounds with amino groups.Despite certain advantages based on Maillard reaction pro...Maillard reaction(MR)is a non-enzymatic browning reaction commonly seen in food processing,which occurs between reducing sugars and compounds with amino groups.Despite certain advantages based on Maillard reaction products(MRPs)found in some food for health and storage application have appeared,however,the MR occurring in human physiological environment can produce advanced glycation end products(AGEs)by non-enzymatic modification of macromolecules such as proteins,lipids and nucleic acid,which could change the structure and functional activity of the molecules themselves.In this review,we take AGEs as our main object,on the one hand,discuss physiologic aging,that is,age-dependent covalent cross-linking and modification of proteins such as collagen that occur in eyes and skin containing connective tissue.On the other hand,pathological aging associated with autoimmune and inflammatory diseases,neurodegenerative diseases,diabetes and diabetic nephropathy,cardiovascular diseases and bone degenerative diseases have been mainly proposed.Based on the series of adverse effects of accelerated aging and disease pathologies caused by MRPs,the possible harm caused by some MR can be slowed down or inhibited by artificial drug intervention,dietary pattern and lifestyle control.It also stimulates people's curiosity to continue to explore the potential link between the MR and human aging and health,which should be paid more attention to for the development of life sciences.展开更多
Over the past century,age-related diseases,such as cancer,type-2 diabetes,obesity,and mental illness,have shown a significant increase,negatively impacting overall quality of life.Studies on aged animal models have un...Over the past century,age-related diseases,such as cancer,type-2 diabetes,obesity,and mental illness,have shown a significant increase,negatively impacting overall quality of life.Studies on aged animal models have unveiled a progressive discoordination at multiple regulatory levels,including transcriptional,translational,and post-translational processes,resulting from cellular stress and circadian derangements.The circadian clock emerges as a key regulator,sustaining physiological homeostasis and promoting healthy aging through timely molecular coordination of pivotal cellular processes,such as stem-cell function,cellular stress responses,and inter-tissue communication,which become disrupted during aging.Given the crucial role of hypothalamic circuits in regulating organismal physiology,metabolic control,sleep homeostasis,and circadian rhythms,and their dependence on these processes,strategies aimed at enhancing hypothalamic and circadian function,including pharmacological and non-pharmacological approaches,offer systemic benefits for healthy aging.Intranasal brain-directed drug administration represents a promising avenue for effectively targeting specific brain regions,like the hypothalamus,while reducing side effects associated with systemic drug delivery,thereby presenting new therapeutic possibilities for diverse age-related conditions.展开更多
Malaria is uncontrolled burden in the world till now.Despite of different efforts to develop antimalarial drug for decades,any anti-malarial drug can able to eradicate completely till now.Many anti-malarial substances...Malaria is uncontrolled burden in the world till now.Despite of different efforts to develop antimalarial drug for decades,any anti-malarial drug can able to eradicate completely till now.Many anti-malarial substances are practically ineffectual because of their physicochemical limitations,cytotoxicity,chemical instability and degradation,and limited activities against intracellular parasites.Taking into consideration,the amount of research is going to conduct in the field of nanoparticle based drug delivery systems,lead to new ways of improving the treatment of infectious diseases.The study has focused on the progress and advancement of research on nanotechnology based drug delivery to eradicate the malaria.We like to focus the efficacy of nanotechnology based drug application for the opening out of novel chemotherapeutics in laboratory research,which may show the way to better use with age-old antimalarial drug and may draw the attention of pharmaceutical industries for the improvement and designing of effective anti-malarial drugs in future.展开更多
This paper aims to describe the socioeconomic and epidemiological profile, as well as the standards of medicine consumption in a group of 154 elderly people from five homes for the aged in Brasilia, in order to expand...This paper aims to describe the socioeconomic and epidemiological profile, as well as the standards of medicine consumption in a group of 154 elderly people from five homes for the aged in Brasilia, in order to expand the understanding about some characteristics and individual needs of this population and its influence on the quality of drug’s therapy. Data were collected between January and December of 2007, it was used a questionnaire adapted from Dader’s method (2002) and a pharmacotherapeutic follow up method and it was responded by the elders and caregivers. Data were supplemented with information from medical records and prescriptions available in the institutions. The studied group has an average age of 74.6 years, living in their current homes for about 5.4 years and the group consists mainly of men with preserved cognitive status. The members of the group have low monthly income, low education level and are sedentary. They consume 4 - 5 drugs and are affected mainly by cardiovascular and psychiatric diseases. Results suggest that low monthly income, low education level, the prevalence of sedentary lifestyle, elders with compromised cognitive status, the increasing number of chronic diseases and the high consumption of drugs in the researched group may be important factors for the emergence or injury drugs-related problems (DRP) as non-adherence to treatment, medication errors, drug interactions and adverse drug reactions compromising the quality of medication therapy. This study points out the necessity of including a pharmacotherapeutic follow-up for the elderly people in order to minimize such problems and provide better quality of life for patients.展开更多
Neurodegenerative disorders affect millions of people worldwide,and the prevalence of these disorders is only projected to rise as the number of people over 65 will drastically increase in the coming years.While thera...Neurodegenerative disorders affect millions of people worldwide,and the prevalence of these disorders is only projected to rise as the number of people over 65 will drastically increase in the coming years.While therapies exist to aid in symptomatic relief,effective treatments that can stop or reve rse the progress of each neurodegenerative disease are lacking.Recently,research on the role of extracellular vesicles as disease markers and therapeutics has been intensively studied.Exosomes,30-150 nm in diameter,are one type of extracellular vesicles facilitating cell-to-cell communication.Exosomes are thought to play a role in disease propagation in a variety of neurodegenerative diseases,such as Alzheimer's disease,Parkinson s disease,and amyotrophic lateral sclerosis.Accordingly,the exosomes derived from the patients are an invaluable source of disease biomarkers.On the other hand,exosomes,especially those derived from stem cells,could serve as a therapeutic for these disorders,as seen by a rapid increase in clinical trials investigating the therapeutic efficacy of exosomes in different neurological diseases.This review summarizes the pathological burden and therapeutic approach of exosomes in neurodegenerative disorders.We also highlight how heat shock increases the yield of exosomes while still maintaining their therapeutic efficacy.Finally,this review concludes with outstanding questions that remain to be addressed in exosomal research.展开更多
目的:探究ATF5蛋白表达水平与胃癌AGS细胞药敏水平的相关性。方法:采用脂质体载体转染技术转染ATF5 pc DNA3.1质粒、ATF5 si RNA空质粒,构建ATF5蛋白高、中、低表达水平的AGS细胞组。Western blot实验检测AGS细胞中ATF5蛋白表达水平,MT...目的:探究ATF5蛋白表达水平与胃癌AGS细胞药敏水平的相关性。方法:采用脂质体载体转染技术转染ATF5 pc DNA3.1质粒、ATF5 si RNA空质粒,构建ATF5蛋白高、中、低表达水平的AGS细胞组。Western blot实验检测AGS细胞中ATF5蛋白表达水平,MTT实验检测AGS细胞对紫杉醇、顺铂药物敏感性,平板克隆形成实验探讨AGS细胞集落形成差异,分析ATF5蛋白表达水平与胃癌AGS细胞药敏水平的相关性。结果:ATF5蛋白过表达组AGS细胞在紫杉醇、顺铂影响后存活率较对照组高,均P<0.05,IC50值较对照组也明显升高,均P=0.00;而低表达组存活率较对照组低,均P<0.05,IC50值较对照组也明显降低,P顺=0.00,P紫=0.002。过表达组细胞在药物影响后克隆形成数较对照组高,而低表达组克隆形成集落较对照组低,均P<0.05。结论:ATF5蛋白的过表达能降低胃癌AGS细胞对紫杉醇、顺铂的药物敏感性,而ATF5蛋白的低表达则能增加胃癌AGS细胞对紫杉醇、顺铂的药物敏感性,提示胃癌AGS细胞药敏水平可能与ATF5蛋白表达水平相关。展开更多
目的探索药品说明书适老化的研究热点,为我国药品说明书适老化发展提供依据。方法在Web of Science核心合集数据库中检索2012-2022年发表的老年人使用药品说明书的相关英文文献,应用VOSviewer和CiteSpace软件进行文献计量学分析,探索该...目的探索药品说明书适老化的研究热点,为我国药品说明书适老化发展提供依据。方法在Web of Science核心合集数据库中检索2012-2022年发表的老年人使用药品说明书的相关英文文献,应用VOSviewer和CiteSpace软件进行文献计量学分析,探索该领域的研究热点,并总结该领域发展的障碍和解决措施。结果与结论本研究共收集到药品说明书适老化相关文献335篇,来自51个国家(地区)的819个研究机构,涉及2174位作者。近10年,药品说明书适老化研究发展趋缓,美国和日本等发达国家占该领域的主导地位,其中美国西北大学Wolf等学者的发文量最大(12篇)。老年人用药风险管理、药品说明书中老年用药信息的更新,以及老年人对药品说明书的理解和依从及其影响因素等是该领域的研究热点。药品说明书适老化发展中相关障碍的解决措施包括提高药品说明书的可视性、可读性,填补药品说明书中老年用药信息等。我国可借鉴其他国家的经验和方法,针对我国人口特点开展老年药品说明书影响因素的调查及老年用药的药动学研究,多维度提高老年患者用药的安全性。展开更多
基金financially supported by grants from the National Natural Science Foundation of China (82170873,81871095)the National Natural Science Foundation of China (81974503)the Tsinghua University Spring Breeze Fund (20211080005)。
文摘Maillard reaction(MR)is a non-enzymatic browning reaction commonly seen in food processing,which occurs between reducing sugars and compounds with amino groups.Despite certain advantages based on Maillard reaction products(MRPs)found in some food for health and storage application have appeared,however,the MR occurring in human physiological environment can produce advanced glycation end products(AGEs)by non-enzymatic modification of macromolecules such as proteins,lipids and nucleic acid,which could change the structure and functional activity of the molecules themselves.In this review,we take AGEs as our main object,on the one hand,discuss physiologic aging,that is,age-dependent covalent cross-linking and modification of proteins such as collagen that occur in eyes and skin containing connective tissue.On the other hand,pathological aging associated with autoimmune and inflammatory diseases,neurodegenerative diseases,diabetes and diabetic nephropathy,cardiovascular diseases and bone degenerative diseases have been mainly proposed.Based on the series of adverse effects of accelerated aging and disease pathologies caused by MRPs,the possible harm caused by some MR can be slowed down or inhibited by artificial drug intervention,dietary pattern and lifestyle control.It also stimulates people's curiosity to continue to explore the potential link between the MR and human aging and health,which should be paid more attention to for the development of life sciences.
基金supported by National Council of Science and Technology(CONACYT)(grants FC 2016/2672 and FOSISS 272757),INMEGEN(09/2017/I)the Ministry of Education,Science,Technology and Innovation of Mexico City(SECTEI)(grant 228/2021).
文摘Over the past century,age-related diseases,such as cancer,type-2 diabetes,obesity,and mental illness,have shown a significant increase,negatively impacting overall quality of life.Studies on aged animal models have unveiled a progressive discoordination at multiple regulatory levels,including transcriptional,translational,and post-translational processes,resulting from cellular stress and circadian derangements.The circadian clock emerges as a key regulator,sustaining physiological homeostasis and promoting healthy aging through timely molecular coordination of pivotal cellular processes,such as stem-cell function,cellular stress responses,and inter-tissue communication,which become disrupted during aging.Given the crucial role of hypothalamic circuits in regulating organismal physiology,metabolic control,sleep homeostasis,and circadian rhythms,and their dependence on these processes,strategies aimed at enhancing hypothalamic and circadian function,including pharmacological and non-pharmacological approaches,offer systemic benefits for healthy aging.Intranasal brain-directed drug administration represents a promising avenue for effectively targeting specific brain regions,like the hypothalamus,while reducing side effects associated with systemic drug delivery,thereby presenting new therapeutic possibilities for diverse age-related conditions.
文摘Researches in recent years have shown that cellular immune factor plays an important role in the generation and development of cerebral hemorrhage1-3.
文摘Malaria is uncontrolled burden in the world till now.Despite of different efforts to develop antimalarial drug for decades,any anti-malarial drug can able to eradicate completely till now.Many anti-malarial substances are practically ineffectual because of their physicochemical limitations,cytotoxicity,chemical instability and degradation,and limited activities against intracellular parasites.Taking into consideration,the amount of research is going to conduct in the field of nanoparticle based drug delivery systems,lead to new ways of improving the treatment of infectious diseases.The study has focused on the progress and advancement of research on nanotechnology based drug delivery to eradicate the malaria.We like to focus the efficacy of nanotechnology based drug application for the opening out of novel chemotherapeutics in laboratory research,which may show the way to better use with age-old antimalarial drug and may draw the attention of pharmaceutical industries for the improvement and designing of effective anti-malarial drugs in future.
文摘This paper aims to describe the socioeconomic and epidemiological profile, as well as the standards of medicine consumption in a group of 154 elderly people from five homes for the aged in Brasilia, in order to expand the understanding about some characteristics and individual needs of this population and its influence on the quality of drug’s therapy. Data were collected between January and December of 2007, it was used a questionnaire adapted from Dader’s method (2002) and a pharmacotherapeutic follow up method and it was responded by the elders and caregivers. Data were supplemented with information from medical records and prescriptions available in the institutions. The studied group has an average age of 74.6 years, living in their current homes for about 5.4 years and the group consists mainly of men with preserved cognitive status. The members of the group have low monthly income, low education level and are sedentary. They consume 4 - 5 drugs and are affected mainly by cardiovascular and psychiatric diseases. Results suggest that low monthly income, low education level, the prevalence of sedentary lifestyle, elders with compromised cognitive status, the increasing number of chronic diseases and the high consumption of drugs in the researched group may be important factors for the emergence or injury drugs-related problems (DRP) as non-adherence to treatment, medication errors, drug interactions and adverse drug reactions compromising the quality of medication therapy. This study points out the necessity of including a pharmacotherapeutic follow-up for the elderly people in order to minimize such problems and provide better quality of life for patients.
基金supported by the National Institute on Aging of NIH(No.RF1AG072510 to HW)the National Institute of General Medical Sciences(NINGM)of NIH(No.P20GM103443 to HW via Dr.Victor Huber)+1 种基金the National Science Foundation(NSF)(No.DGE-1633213 to CCH via Dr.Brian Burrell)the NIH/NIGMS(No.T32GM-136503 to CCH via Dr.Brian Burrell)。
文摘Neurodegenerative disorders affect millions of people worldwide,and the prevalence of these disorders is only projected to rise as the number of people over 65 will drastically increase in the coming years.While therapies exist to aid in symptomatic relief,effective treatments that can stop or reve rse the progress of each neurodegenerative disease are lacking.Recently,research on the role of extracellular vesicles as disease markers and therapeutics has been intensively studied.Exosomes,30-150 nm in diameter,are one type of extracellular vesicles facilitating cell-to-cell communication.Exosomes are thought to play a role in disease propagation in a variety of neurodegenerative diseases,such as Alzheimer's disease,Parkinson s disease,and amyotrophic lateral sclerosis.Accordingly,the exosomes derived from the patients are an invaluable source of disease biomarkers.On the other hand,exosomes,especially those derived from stem cells,could serve as a therapeutic for these disorders,as seen by a rapid increase in clinical trials investigating the therapeutic efficacy of exosomes in different neurological diseases.This review summarizes the pathological burden and therapeutic approach of exosomes in neurodegenerative disorders.We also highlight how heat shock increases the yield of exosomes while still maintaining their therapeutic efficacy.Finally,this review concludes with outstanding questions that remain to be addressed in exosomal research.
文摘目的:探究ATF5蛋白表达水平与胃癌AGS细胞药敏水平的相关性。方法:采用脂质体载体转染技术转染ATF5 pc DNA3.1质粒、ATF5 si RNA空质粒,构建ATF5蛋白高、中、低表达水平的AGS细胞组。Western blot实验检测AGS细胞中ATF5蛋白表达水平,MTT实验检测AGS细胞对紫杉醇、顺铂药物敏感性,平板克隆形成实验探讨AGS细胞集落形成差异,分析ATF5蛋白表达水平与胃癌AGS细胞药敏水平的相关性。结果:ATF5蛋白过表达组AGS细胞在紫杉醇、顺铂影响后存活率较对照组高,均P<0.05,IC50值较对照组也明显升高,均P=0.00;而低表达组存活率较对照组低,均P<0.05,IC50值较对照组也明显降低,P顺=0.00,P紫=0.002。过表达组细胞在药物影响后克隆形成数较对照组高,而低表达组克隆形成集落较对照组低,均P<0.05。结论:ATF5蛋白的过表达能降低胃癌AGS细胞对紫杉醇、顺铂的药物敏感性,而ATF5蛋白的低表达则能增加胃癌AGS细胞对紫杉醇、顺铂的药物敏感性,提示胃癌AGS细胞药敏水平可能与ATF5蛋白表达水平相关。
文摘目的探索药品说明书适老化的研究热点,为我国药品说明书适老化发展提供依据。方法在Web of Science核心合集数据库中检索2012-2022年发表的老年人使用药品说明书的相关英文文献,应用VOSviewer和CiteSpace软件进行文献计量学分析,探索该领域的研究热点,并总结该领域发展的障碍和解决措施。结果与结论本研究共收集到药品说明书适老化相关文献335篇,来自51个国家(地区)的819个研究机构,涉及2174位作者。近10年,药品说明书适老化研究发展趋缓,美国和日本等发达国家占该领域的主导地位,其中美国西北大学Wolf等学者的发文量最大(12篇)。老年人用药风险管理、药品说明书中老年用药信息的更新,以及老年人对药品说明书的理解和依从及其影响因素等是该领域的研究热点。药品说明书适老化发展中相关障碍的解决措施包括提高药品说明书的可视性、可读性,填补药品说明书中老年用药信息等。我国可借鉴其他国家的经验和方法,针对我国人口特点开展老年药品说明书影响因素的调查及老年用药的药动学研究,多维度提高老年患者用药的安全性。