Thirty three species of marine algae belonging to Rhodophyta, Phaeophyta and Chlorophyta from the Fujian coast were examined for agglutinins with different animal and human erythrocytes. Protein extracts from 26 speci...Thirty three species of marine algae belonging to Rhodophyta, Phaeophyta and Chlorophyta from the Fujian coast were examined for agglutinins with different animal and human erythrocytes. Protein extracts from 26 species were active against at least one type of the erythrocytes tested. There were 3 species ( Grateloupia imbricata, Ishige foliacea and Entermorpha prolifera ) whose extracts could agglutinate all the erythrocytes used. The lowest protein concentration required to produce erythrocyte agglutination varied remarkably, from 3.1 μg/ml to 500 μg/ml . The strongest activity was found in the agglutination of rabbit erythrocytes by Gloiopeltis furcata extract. Inhibition assays performed with nine mono and bisaccharides indicated that agglutinations of rabbit erythrocytes by extracts of 7 species were inhibited by one or more types of the sugars assayed. The agglutinating activity shown by extracts of most species was not affected when the test solution was heated to 90℃, but was lost at 95℃-100℃. A few extracts lost their activity at 60℃, 65℃ and 75 ℃, respectively.展开更多
本研究旨在建立体外溶血试验配血方法,指导急性溶血性贫血产妇输血的救治治疗。在体外将患者血浆中所有抗体全部致敏供者红细胞并加入补体,经6.5 h 38℃孵育,观察是否出现溶血现象,不溶血的供血者的血液可以安全输注给受者。结果表明:...本研究旨在建立体外溶血试验配血方法,指导急性溶血性贫血产妇输血的救治治疗。在体外将患者血浆中所有抗体全部致敏供者红细胞并加入补体,经6.5 h 38℃孵育,观察是否出现溶血现象,不溶血的供血者的血液可以安全输注给受者。结果表明:患急性溶血病产妇配血困难时采用体外溶血试验方法筛查到相容供者,给予安全输注去白细胞的红细胞悬液6个单位,无不良反应,输血后患者血红蛋白、总胆红素、间接胆红质、网织红细胞、D-二聚体等指标迅速改善,临床输血治疗获得良好效果。结论:常规交叉配血无法准确判断配血结果是否相容时,采用体外溶血试验能筛查到凝集-不溶血红细胞,输血效果好,血红蛋白提升快,无不良反应。展开更多
文摘Thirty three species of marine algae belonging to Rhodophyta, Phaeophyta and Chlorophyta from the Fujian coast were examined for agglutinins with different animal and human erythrocytes. Protein extracts from 26 species were active against at least one type of the erythrocytes tested. There were 3 species ( Grateloupia imbricata, Ishige foliacea and Entermorpha prolifera ) whose extracts could agglutinate all the erythrocytes used. The lowest protein concentration required to produce erythrocyte agglutination varied remarkably, from 3.1 μg/ml to 500 μg/ml . The strongest activity was found in the agglutination of rabbit erythrocytes by Gloiopeltis furcata extract. Inhibition assays performed with nine mono and bisaccharides indicated that agglutinations of rabbit erythrocytes by extracts of 7 species were inhibited by one or more types of the sugars assayed. The agglutinating activity shown by extracts of most species was not affected when the test solution was heated to 90℃, but was lost at 95℃-100℃. A few extracts lost their activity at 60℃, 65℃ and 75 ℃, respectively.
文摘本研究旨在建立体外溶血试验配血方法,指导急性溶血性贫血产妇输血的救治治疗。在体外将患者血浆中所有抗体全部致敏供者红细胞并加入补体,经6.5 h 38℃孵育,观察是否出现溶血现象,不溶血的供血者的血液可以安全输注给受者。结果表明:患急性溶血病产妇配血困难时采用体外溶血试验方法筛查到相容供者,给予安全输注去白细胞的红细胞悬液6个单位,无不良反应,输血后患者血红蛋白、总胆红素、间接胆红质、网织红细胞、D-二聚体等指标迅速改善,临床输血治疗获得良好效果。结论:常规交叉配血无法准确判断配血结果是否相容时,采用体外溶血试验能筛查到凝集-不溶血红细胞,输血效果好,血红蛋白提升快,无不良反应。
