Therapeutic Effect and Mechanism of New Maixian Powder on DSS-induced UC Rats

[Objectives] To study the therapeutic effect and mechanism of New Maixian Powder on ulcerative colitis( UC) rats through observing its regulatory effect on the protein kinase R-like endoplasmic reticulum kinase( PERK)/eukaryotic translation initiation factor-2α( e IF-2α)/nuclear transcription factor-kappa B( NF-κB) signaling pathway. [Methods]First,60 SD rats were randomly divided into normal group,model group,mesalazine group,and New Maixian Powder low,medium and high dose groups,10 rats each group. Then,dextran sulfate sodium( DSS) was used to induce UC rats. The mesalazine group was given 0. 42 g/( kg·d) of mesalazine sustained-release granule suspension,New Maixian Powder low,medium and high dose groups were given 1. 5,3,and 6 g/( kg·d) of New Maixian Powder suspension,respectively,and other groups were given an equal volume of physiological saline,continuous intragastric administration for 14 d. Next,the disease activity index( DAI) of UC rats was evaluated; the expression of NF-κB in serum was measured by enzyme-linked immunosorbent assay( ELISA); the expression of PERK and e IF-2α protein and m RNA in colon tissue was detected by Western blot and real-time quantitative polymerase chain reaction( RT q-PCR). [Results] Compared with the normal group,the DAI score and serum NF-κB level in the model group were significantly higher( P < 0. 05),and PERK and e IF-2α protein and m RNA levels in the colon tissue were increased( P < 0. 05); compared with the model group,the DAI score decreased and serum NF-κB level declined in the New Maixian Powder group,and the expression of PERK and e IF-2α protein and m RNA in New Maixian Powder medium dose and high dose groups declined( P < 0. 05). [Conclusions]New Maixian Powder has good therapeutic effect on UC rats,and its mechanism may be connected with the inhibition of the activation of PERK/e IF-2α/NF-κB signaling pathway.

容器运动型混合机在我国发展现状及展望

容器运动型混合机是一种应用广泛的粉体混合机.介绍了摇滚式混合机和摇摆式混合机近年来在我国发展状况,并着重介绍了有关摇滚式混合机和摇摆式混合机的结构及运动特点.由于传统的粉体混合机分类未包括此类混合机,建议混合机分类方法进行必要的改进.

气力输送装置在燃烧脱硫技术中的应用

在SHL10-1.27-A链条炉上进行了燃烧脱硫技术性示范工业实验,气力输粉装置连续稳定运行是喷钙脱硫技术成功的关键,介绍了其设计、安装和调整实验及其影响正常运行的各种因素。试验证明该装置能成功地输送钙基粉状脱硫剂。

海南特产英文翻译指瑕及对策——以椰子粉的英文翻译为例

文章对海南特产椰子粉的英文翻译存在的瑕疵或错误进行指瑕,分析造成英文翻译的瑕疵或错误原因,提出提高思想认识、专业人员做翻译、工商管理部门联合监督、编制出版海南特产英文翻译专用术语手册等策略。

柴胡疏肝散对功能性消化不良大鼠胃排空的促进作用及机制

目的观察柴胡疏肝散对功能性消化不良(FD)大鼠胃排空的促进作用,并探讨其作用机制。方法将24只健康成年SD大鼠随机均分为正常组、模型组、柴胡疏肝散组与多潘力酮组。除正常组外,其余各组均采用夹尾刺激法制备FD模型。各组均于造模第1天给予药物干预,柴胡疏肝散组、多潘立酮组分别给予0.63 g/mL柴胡疏肝散水煎剂1.5 mL、0.6 mg/mL多潘立酮水溶液1.5 mL灌胃,正常组、模型组均给予生理盐水1.5 mL灌胃,2次/d,共干预4周。末次给药后24 h,各组予以营养性半固体糊(1.5 mL/100 g体质量)灌胃,30 min后麻醉开腹,剪取完整胃,根据全胃重、空胃重计算胃排空率。用HE染色法观察胃窦部黏膜组织病理变化,用免疫组化法检测胃窦部黏膜组织中蛋白激酶R样内质网激酶(PERK)和磷酸化真核翻译起始因子2α(p-eIF2α)蛋白表达。结果与正常组比较,模型组大鼠胃排空率低(P<0.05);与模型组比较,柴胡疏肝散组、多潘立酮组胃排空率高(P均<0.05)。模型组、柴胡疏肝散组和多潘立酮组胃窦组织可见少量淋巴细胞及中性粒细胞浸润。与正常组比较,模型组大鼠胃窦组织内PERK、p-eIF2α蛋白表达高(P均<0.05);与模型组比较,柴胡疏肝散组、多潘立酮组胃窦组织内PERK、p-eIF2α蛋白表达低(P均<0.05)。结论柴胡疏肝散可能通过调控内质网应激信号通路PERK/eIF2α促进FD大鼠胃排空。

新麦纤散对DSS诱导UC大鼠的治疗作用及机制分析

目的：观察新麦纤散对溃疡性结肠炎（ulcerative colitis,UC）大鼠蛋白激酶R样内质网激酶（PERK）/真核翻译起始因子-2α（eIF-2α）/核转录因子-κB（NF-κB）信号通路的调控作用,探究其对UC的疗效和作用机制。方法：将60只SD大鼠随机分成正常组、模型组、美沙拉嗪组、新麦纤散低、中、高剂量组,每组10只,采用葡聚糖硫酸钠（dextran sulfate sodium,DSS）成功诱导UC模型后,美沙拉嗪组给予美沙拉嗪缓释颗粒混悬液0.42 g·kg^（-1）·d^（-1）,新麦纤散低、中、高剂量组分别给予新麦纤散混悬液1.5,3,6 g·kg^（-1）·d^（-1）,其余组给予等体积生理盐水,连续灌胃14 d。评估UC大鼠疾病活动指数（DAI）,酶联免疫吸附法（ELISA）检测血清NF-κB表达水平,蛋白免疫印迹法（Western blot）和实时荧光定量聚合酶链式反应法（Real-time PCR）分别观察结肠组织PERK,eIF-2α蛋白和mRNA的表达。结果：与正常组比较,模型组DAI评分和血清NF-κB水平显著升高（P〈0.05）,结肠组织PERK,eIF-2α蛋白和mRNA水平均升高（P〈0.05）;与模型组比较,新麦纤散组DAI评分减少,血清NF-κB水平降低（P〈0.05）,新麦纤散中、高剂量组PERK,eIF-2α蛋白和mRNA的表达均降低（P〈0.05）。结论：新麦纤散对UC大鼠有较好的疗效,其作用机制可能与抑制PERK/eIF-2α/NF-κB信号通路的激活有关。