SYNTHESIS AND CHARACTERIZATION OF A NOVEL ALCOHOL-SOLUBLE POLYORGANOSILOXANE CONTAINING AMINO SIDE GROUPS

A novel alcohol-soluble polyorganosiroxane containing amino side groups, poly[3-(4-aminophenoxy)-aminophenoxy) propylmethylsiloxane] (2), was first prepared by the reduction of nitro groups on 4-nitrophenoxypropyl side chains of poly[3-(4-nitrophenoxy)propylmethylsiloxane] (1). The reaction proceeded easily with nearly 100% conversion. The synthesized alcohol-soluble polymer 2, which has potential application as a precursor for preparing advanced functional polymers, was characterized by FTIR, H-1-NMR, C-13-NMR, Si-29-NMR, VPO and GPC, respectively.

Glycosylation-independent binding to extracellular domains 11-13 of mannose-6-phosphate/insulin-like growth factor-2 receptor mediates the effects of soluble CREG on the phenotypic proliferation of vascular smooth muscle cells

Background The present study aimed to investigate the detailed mode and specific sites for their binding as well as the functional relevance of this binding in the phenotypic proliferation of vascular smooth muscle cells(SMCs). Methods CREG knocked-down SMCs were employed to evaluate the biological activity of wtCREG and mCREG.Expressions of SMC differentiation markers SM myosin heavy chain(SM-MHC),SM-actin,heavy caldesmon and myocardin were determined by Western blotting using specific antibodies. Cellular growth of SMCs was assessed by bromide dewuridine (BrdU) incorporation and cell cycle analysis on fluorescence-activated cell sorting(FACS).A solid-phase binding assay was used to study the binding of CREG to extracellular domains of M6P/IGF2R.The cellular co-localization of the two recombinant CREGs with M6P/IGF2R was detected on SMC surface by immunoprecipitation and immunofluorescence analysis.Results The molecular weight of wtCREG was around 30 kD while that of the mCREG was～25 kD.Treatment of wtCREG with PNGase F reduced its molecular weight from～30 kD to～25 kD,whereas PNGase F treatment had no effect on the molecular weight of mCREG.Both wtCREG and mCREG proteins enhanced SMC differentiation,inhibited BrdU incorporation,and arrested cell cycle progression when added to the culture medium.In CREG knocked-down SMCs,the amount of CREG detected by immunoblotting in M6P/IGF2R immunoprecipitates was significantly reduced when compared to normal cells.Both recombinant CREGs co-immunoprecipitated with M6P/IGF2R, although slightly reduced amount of the mutant CREG was detected in M6P/IGF2R immunoprecipitates.Immunostaining revealed that His-tagged CREGs co-localized with IGF2R on the cell surface in a glycosylation-independent manner.In vitro binding assay showed that CREGs bound to M6P/ IGF2R extracellular domains 7-10 and 11-13 in a glycosylation -dependent and -independent manner,respectively.Further blocking experiments using soluble M6P/IGF2R fragments and M6P/IGF2R neutralizing antibody indicated that the biological activities of recombinant CREGs in SMC growth and the up-regulation of SMC differentiation markers were all abolished by treatment with the M6P/IGF2R neutralizing antibody. However,although the growth inhibitory effect of wtCREG was nearly abolished by D7-10 or D11-13,the effect of mCREG was only reversed by Dll-13,indicating that the binding to domains 11-13 is required for CREG to modulate the proliferation of SMCs.Conclusions These data suggest that solubleCREG proteins can exert their biological function via binding to the extracellular domains 7-10 and 11-13 of cell surface M6P/IGF2R in both a glycosylation-dependent and -independent manner.

Physicochemical Properties of Poly(Vinyl Alcohol)/Chitosan/Soluble Starch Composite Hydrogel

Fabricating of hydrogels based upon polymers and inorganic matter is an innovative replacement for the generation of adaptable matrices.In this study,the poly(vinyl alcohol)(PVA)/chitosan(CS)/soluble starch(SS)composite hydrogel was prepared by the solution blending method,in which SS was used as a cross-linking agent to cross-link with PVA in order to improve the stability of PVA in the tissue fluid.The composition and the thermal stability of hydrogels were characterized by Fourier transform infrared(FTIR)spectroscopy and thermo gravimetric analysis(TGA).The result shows that the PVA/CS/SS composite hydrogel possesses potentially morphological and thermal stability and can be used for tissue engineering.

Elevated soluble 4-1BB is associated with serum markers of hepatitis B virus in patients with chronic hepatitis B

BACKGROUND Previous studies have suggested that the costimulatory molecule 4-1BB plays pivotal roles in regulating immunity during chronic viral infection.However,up to now,there are few studies about 4-1BB in chronic hepatitis B(CHB).AIM To clarify this issue,we report our comprehensive study results on the expression levels of 4-1BB in patients with CHB.METHODS From September 2018 to June 2019,a total of 64 patients with CHB were recruited from the Department of Hepatology,The First Hospital of Jilin University.Peripheral blood samples were collected from 52 treatment-naïve and 12 entecavir-treated patients with CHB as well as 37 healthy donors(including 24 healthy adults and 13 healthy children).The levels of soluble 4-1BB(s4-1BB)in plasma were measured by ELISA.4-1BB mRNA expression in peripheral blood mononuclear cells was detected by real-time quantitative PCR.RESULTS The s4-1BB levels in the plasma of patients with CHB were significantly higher than those in healthy adults(94.390±7.393 ng/mL vs 8.875±0.914 ng/mL,P<0.001).In addition,the s4-1BB level in plasma was significantly increased in patients with a higher viral load and a disease flare up.However,there were no significant differences between treatment-naïve and entecavir-treated patients.Interestingly,among treatment-naïve patients with CHB,the levels of s4-1BB in plasma had a significant positive correlation with hepatitis B surface antigen,hepatitis B virus DNA,hepatitis B e antigen,and triglyceride levels(r=0.748,P<0.001;r=0.406,P=0.004;r=0.356,P=0.019 and r=-0.469,P=0.007,respectively).The 4-1BB mRNA expression was higher in the peripheral blood mononuclear cells of patients with CHB than in the peripheral blood mononuclear cells of healthy adults,but the difference was not statistically significant.CONCLUSION These results suggest that the levels of s4-1BB may be associated with pathogenesis of hepatitis B virus and therefore may be a promising biomarker for disease progression.

DEVELOPMENT OF A CATALYST FOR SOLUTION OF POLY(VINYL ALCOHOL) IN NON-AQUEOUS MEDIUM

Chloro ethane dimethyl sulfoxide,C_2H_5Cl·DMSO(ECI·DMSO)was prepared by interaction of acrylic acid with conc.Hydrochloric acid in dimethyl sulfoxide(DMSO)and subsequent decarboxylation with H_2O_2 solution.The formation of the compound was confirmed by spectral and analytical methods;the molecular weight was determined by cryoscopic method.The solubility of poly(vinyl alcohol)(PVA)in different solvents or mixed solvents at 40℃,50℃and 60℃temperature in the presence of 0.01% of EC1·-DMSO was determined.It tu...

Comparative Studies on Mass Spectrometric Fragmentation of Linear Chiral Secondary Alcohols (R)-1-(4-Alkylphenyl) and (R)-1-(4-Alkoxyphenyl/Alkylthiophenyl) Alcohols

Mass spectrometric behaviour of （R） -1- （ 4-alkylphenyl ） alcohols, 1- （4-alkoxylphenyl） alcohols, and 1- （4-alkylthiophenyl） alcohols were studied with the aid of mass-analyzed ion kinetic energy spectrometry under electron impact ionization. All the title compounds show a tendency to eliminate a water molecule to form alkene ions and undergo an a-cleavago to produce protonated aldehyde ions by the loss of alkyl radicals. Except these two common fragment ions, they also show some different fragmentations due to with or without oxy/thioether-linkage.

Enantiomeric Separation of Amino Alcohols by Ion-pair Chromatography

Enantiomers of four amino alcohols were resolved by ion-pair chromatography with (+)-10-camphorsulphonic acid as chiral counter ion. Studies of the influence of the mobile phase composition, the solute structure and the mobile phase flow-rate on separation are presented. Under the optimized conditions enantiomeric propanolol, norephedrine, metropolol and salbutamol were separated using dichloromethane-1-pentanol (97:3 v/v) as mobile phase on Lichrospher-100-DIOL column.

Combination of specific monoclonal antibodies allow identification of soluble aggregates of by sandwich ELISA

Aggregate amyloid beta protein1-42 (Aβ1-42) can typically be found in the early stage of Alzheimer’s disease (AD). Aβ1-42 self-assembles and is highly toxic to neurons. Thus, recognizing aggregated Aβ1-42 is very important for elucidation of Aβ1-42 structure and for the diagnosis of AD. In this study, the specificity of the 79-3 monoclonal antibody against soluble aggre- gate Aβ1-42 was measured by sandwich Enzyme-Linked Immuno Sorbent Assay (ELISA). Eight monoclonal antibodies against both soluble aggregates and amorphous aggregates were used as primary antibodies. Soluble aggregates and amorphous aggregates were used as antigen. As secondary antibody, HRP was labeled with the 79-3 monoclonal antibody. The reactivity of the 79-3 monoclonal antibody against soluble aggregates was confirmed in all combinations, but little reactivity against amorphous aggregates was found. Furthermore, we performed the above sandwich ELISA using the 37-11 antibody, which is reactive against large oval aggregates (LOA) that occur in micro aggregates, instead of the 79-3 antibody. The 77-3 antibody is 1 of the 8 monoclonal antibodies against soluble aggregates;amorphous aggregates also reacted with the 37-11 antibody. These results indicated that soluble aggregates are specifically recognized by a combination of different antibodies. The combined use of these antibodies can be applied to the diagnosis of AD and to defining the structure of the Aβ1-42.

Isobaric Vapor–Liquid Equilibrium for tert-Butyl Alcohol + Water + Propane-1,3-Diol + 1-Ethyl-3-Methylimidazolium Chloride at 101.3 kPa

In this study, we used a mixture of organic liquid propane-1,3-diol and ionic liquid 1-ethyl-3-methylimidazolium chloride([emim]Cl) as the entrainer to separate tert-butyl alcohol(TBA) + water. We measured the isobaric vapor–liquid equilibrium(VLE) for the quaternary system TBA + water + propane-1,3-diol + [emim]Cl at 101.3 kPa, and found the VLE data to be well correlated with the nonrandom two-liquid model. These results show that the mixed solvent of propane-1,3-diol + [emim]Cl can increase the relative volatility of TBA to water and break the azeotropic point. We found no notable synergetic effect between them, and observed that the liquid mixed solvent of propane-1,3-diol and [emim]Cl had lower viscosity than [emim]Cl, which makes it a promising entrainer for separating the TBA + water azeotrope in industrial applications.

纳米SiO_2改性醇溶性蓖麻油-聚氨酯树脂的制备与表征

利用端氨基偶联剂KH-550对纳米SiO2进行表面改性,在超声场作用下制备疏水性纳米SiO2粒子,并将其均匀分散到蓖麻油(CO)-聚氨酯溶液,通过红外光谱(IR)、热重分析(TGA)和扫描电镜(SEM)等测试方法,探讨醇溶性聚氨酯的热稳定性、铅笔硬度和胶膜力学性能等。红外分析表明,PU大分子和纳米SiO2之间形成化学键,形成了Si—O—Si结构。当蓖麻油添加量为多元醇总质量的10%时,拉伸强度由原来的6.8 MPa增加到16.2 MPa,吸水率降低至原来的1/2。纳米SiO2颗粒掺杂量由0%增加到2%时,PU涂膜的失重速率最高点温度由原来的345℃提高至369℃,热分解温度也有所提高。涂膜热重曲线出现右移。

超声-微波协同酶法改性小米糠膳食纤维及工艺优化

为提高小米糠水溶性膳食纤维(Soluble dietary fiber,SDF)的得率,本实验以小米糠为材料,对其进行气爆预处理,利用超声-微波协同酶法对气爆预处理小米糠进行改性,分别研究酶添加量、温度、pH、微波功率对小米糠SDF含量的影响,根据单因素实验结果设计Box-Behnken实验,采用响应面法优化改性小米糠SDF的工艺条件。结果表明:气爆条件设定为压力1.0 MPa、时间90 s,最优工艺参数为酶添加量5.85%,温度56℃,pH4.64,微波功率451 W,在此条件下改性小米糠SDF含量为13.117%,比未经改性小米糠SDF含量高出了10.96%。

应用A-PAGE电泳方法鉴定豇豆品种

分别用3种提取液提取种子醇溶蛋白,利用改进的ISTA小麦和大麦醇溶蛋白酸性聚丙烯酰胺凝胶电泳(A-PAGE)方法对豇豆不同品种种子进行了鉴定。结果表明,以20%二氯乙醇+1%2-巯基乙醇+18%的尿素为提取液,且加样量为10μl时,电泳图谱条带清晰,可以对豇豆品种进行有效地鉴定。利用超声波处理样品可以缩短样品提取时间并得到同样的提取效果。

枸杞子药材醇溶性成分的气相色谱-质谱分析

目的：检测了解枸杞子药材的有关成分，比较各产地药材的异同，便于进行质量控制。方法：取不同产地的枸杞子以乙醇提取后，进行气相色谱质谱（ＧＣＭＳ）分析，对比各药材的总离子流图，分析各组成组分。结果：认为其总离子流图大致相同，可作为定性的依据；但亦存在不同之处，各组分及相对百分含量不尽相同，共鉴定出２２种化合物。主要成分为脂肪酸、糖醛、β谷甾醇等。结论：分析鉴定结果可作为枸杞子药材的初步定性依据，并在此基础上收集大量谱图，建立起较完整的谱库。

O-2'-羟丙基三甲基氯化铵壳聚糖的合成与表征

通过苯甲醛与壳聚糖反应形成Schiff碱 ,对C2 上氨基进行保护 ,经过 2 ,3 环氧丙基三甲基氯化铵与Schiff碱壳聚糖中间体反应后再脱保护 ,合成了O上取代的季铵盐壳聚糖 ,其结构经IR ,1HNMR及13 CNMR分析表征。实验表明O上取代的季铵盐壳聚糖可溶于H2 O及甲醇、乙醇、乙二醇等有机溶剂中。

异丁烯在水-叔丁醇混合溶剂中的溶解度

将叔丁醇加入水中可增加异丁烯气体在水相中的溶解度,提高异丁烯水合反应速率．在压力0．60～0．74 MPa、温度335．15～358．15K、叔丁醇浓度0～2．0kmol/m^3条件下测定了异丁烯在叔丁醇水溶液中的溶解度．结果表明,叔丁醇能增加异丁烯在水中的溶解度,溶解度随温度的升高而降低．依据溶解度与温度及叔丁醇摩尔浓度的实验数据拟合得到异丁烯溶解度的关系式,并讨论了异丁烯在叔丁醇水溶液中溶解的标准偏摩尔焓及偏摩尔熵．

脂溶性维生素注射液联合丁二磺酸腺苷蛋氨酸治疗酒精性肝病伴胆汁淤积患者的效果及对血清sVCAM-1、PLTP水平的影响

目的探究脂溶性维生素注射液联合丁二磺酸腺苷蛋氨酸治疗酒精性肝病伴肝并胆汁淤积的临床疗效及对患者血清可溶性黏附分子(s VCAM-1)、磷脂转运蛋白(PLTP)水平的影响。方法回顾性选取2016年1月至2017年8月收治的140例酒精性肝病伴胆汁淤积患者,依照治疗方法的不同分成对照组和观察组,每组70例。对照组给予丁二磺酸腺苷蛋氨酸治疗,观察组在对照组基础上给予脂溶性维生素注射液联合治疗。对比两组患者的临床疗效、治疗前后血生化指标、结合胆汁酸(CBA)、血清肝胆酸(CG)、s VCAM-1及PLTP差异及症状改善情况。结果观察组的临床总有效率为92. 9%,显著高于对照组的81. 4%,差异具有统计学意义(P <0. 05)。治疗后,两组患者天冬氨酸氨基转移酶(AST)、总胆汁酸(TBA)、γ-谷氨酰转肽酶(GGT)、丙氨酸氨基转移酶(ALT)、总胆红素(TBIL)、Ⅲ型前胶原(PC-Ⅲ)、血清胆红素(SB)、透明质酸酶(HA)及Ⅳ型胶原蛋白(Ⅳ-C)均显著降低,而白蛋白(Alb)显著升高,且观察组各指标相比于对照组变化更显著(P <0. 05)。治疗后,两组患者CBA、CG、s VCAM-1及PLTP水平均显著降低,且观察组更低于对照组,差异具有统计学意义(P <0. 05)。治疗后,两组多数患者症状消失或减弱,且观察组患者更优于对照组(P <0. 05)。结论脂溶性维生素注射液联合丁二磺酸腺苷蛋氨酸治疗酒精性肝病伴胆汁淤积可明显的改善患者肝功能及胆汁酸的代谢,这可能与联合治疗对血清s VCAM-1、PLTP水平的降低作用有关。

超低温(-196℃)保存11年后小麦种子的活力

三种含水量的春小麦品种他诺瑞种子,在超低温(-196℃)保存11年后,种子的发芽势、发芽率同保存前相比,没有明显变化,田间生长正常。经超低温保存的种子根尖细胞有丝分裂后期染色体异常频率在2.00％～3.64％之间,对照种子染色体异常频率为2.45％。保存后,种子的醇溶蛋白质电泳图谱没有发生变化,过氧化物酶同工酶谱同对照相比也没有明显变化,酶带数目相同。只是由于种子含水量不同,其酶带颜色有差异,含水量11.5％的种子,酶带颜色最深,活性最强;含水量5.8％的种子,酶带颜色最浅、活性弱。

塑料复合膜用醇溶性聚丙烯酸酯-聚氨酯胶粘剂的研究

首先合成了含环氧基团的醇溶性聚丙烯酸酯作为主剂,然后合成出氨基封端的聚醚型聚氨酯作为固化剂,通过调节二者的配比来得到双组分醇溶性聚丙烯酸酯-聚氨酯复合胶。用红外(FTIR)、核磁(NMR)和热分析(TG、DTG)表征了主剂与固化剂的结构,并用红外光谱对胶粘剂的固化过程进行了表征,研究了双组分胶的配比对胶粘剂性能的影响。结果表明,该复膜胶具有环保、无毒、剥离强度高和常温快速固化等特点,适用于制备塑料复合薄膜。当主剂/固化剂=10/2(质量比),常温下熟化时间24 h,该复合胶的粘接性能最佳。