Background Tenascin-x, an extracellular matrix glycoprotein exclusively expressed in fibroblasts, can mediate fibrosis in the presence of collagen. Therefore, we have investigated its potential role in facilitating my...Background Tenascin-x, an extracellular matrix glycoprotein exclusively expressed in fibroblasts, can mediate fibrosis in the presence of collagen. Therefore, we have investigated its potential role in facilitating myocardial fibrosis and cardiac remodeling via the transforming growth factor-lβ1 and peroxisome proliferator-activated receptor γ（TGFβ1-PPARγ） pathway in alcoholic cardiomyopathy （ACM）.Methods Experimental animals were divided into control （group A） and tenascin-x knock-out groups （group B）receiving alcohol. Six months post treatment, cardiac ejections fraction （EF）, fractional shortening （FS）, left ventricle end-diastole internal diameter （LVEDd） and collagen column fraction （CVF） were observed. Tenascin-x, smad-3, TGFβ1,smad-7 and PPARγ protein expression levels were detected by Western blotting.Results Six months post treatment, EF and FS values were higher in group B than in group A （P 〈0.05 and P 〈0.01,respectively）, while LVEDd and CVF were lower in group B （P 〈0.05 and P 〈0.01, respectively）. Tenascin-x, smad-3 and TGFβ1 protein expression levels were higher in group A, while smad-7 and PPARY levels were lower than in group B （P〈0.01）, as measured by immunohistochemistry and Western blotting. Tenascin-x protein expression was negatively correlated with EF, FS, smad-7 and PPARγ, and positively correlated with LVEDd, CVF, smad-3, and TGFβ1 （P 〈0.001）.Conclusion Tenascin-x is an initiator of myocardial fibrosis and ACM development via upregulation of TGFβ1 and downregulation of PPARγ.展开更多
Heart failure(HF) following liver transplant(LT) surgery is a distinct clinical entity with high mortality. It is known to occur in absence of obvious risk factors. No preoperative workup including electrocardiogram, ...Heart failure(HF) following liver transplant(LT) surgery is a distinct clinical entity with high mortality. It is known to occur in absence of obvious risk factors. No preoperative workup including electrocardiogram, echocardiography at rest and on stress, reasonably prognosticates the risk. In patients of chronic liver disease, cirrhotic cardiomyopathy, alcoholic cardiomyopathy, and stress induced cardiomyopathy have each been implicated as a cause for HF after LT. However distinguishing one etiology from another not only is difficult, several etiologies may possibly coexist in a given patient. Diagnostic dilemma is further compounded by the fact that presentation and management of HF irrespective of the possible underlying cause, remains the same. In this case series, 6 cases are presented and in the light of existing literature modification in the preoperative workup are suggested.展开更多
Alcoholic cardiomyopathy(ACM)is a dilated cardiomyopathy induced by excessive alcohol consumption that causes progressive cardiac dysfunction and chamber dilatation,leading to arrhythmias,heart failure,and death.Cardi...Alcoholic cardiomyopathy(ACM)is a dilated cardiomyopathy induced by excessive alcohol consumption that causes progressive cardiac dysfunction and chamber dilatation,leading to arrhythmias,heart failure,and death.Cardiac transplantation is the final measure for end-stage ACM.In this case,we used multimodal imaging for ACM diagnosis in a patient with refractory heart failure.The patient received a heart transplant with a good prognosis,and his pathological results after the transplant confirmed our suspected diagnosis.End-stage ACM may lead to refractory heart failure and multimodal imaging may play an important role in the diagnosis,prognosis prediction,and follow-up study of suspected ACM.展开更多
文摘Background Tenascin-x, an extracellular matrix glycoprotein exclusively expressed in fibroblasts, can mediate fibrosis in the presence of collagen. Therefore, we have investigated its potential role in facilitating myocardial fibrosis and cardiac remodeling via the transforming growth factor-lβ1 and peroxisome proliferator-activated receptor γ（TGFβ1-PPARγ） pathway in alcoholic cardiomyopathy （ACM）.Methods Experimental animals were divided into control （group A） and tenascin-x knock-out groups （group B）receiving alcohol. Six months post treatment, cardiac ejections fraction （EF）, fractional shortening （FS）, left ventricle end-diastole internal diameter （LVEDd） and collagen column fraction （CVF） were observed. Tenascin-x, smad-3, TGFβ1,smad-7 and PPARγ protein expression levels were detected by Western blotting.Results Six months post treatment, EF and FS values were higher in group B than in group A （P 〈0.05 and P 〈0.01,respectively）, while LVEDd and CVF were lower in group B （P 〈0.05 and P 〈0.01, respectively）. Tenascin-x, smad-3 and TGFβ1 protein expression levels were higher in group A, while smad-7 and PPARY levels were lower than in group B （P〈0.01）, as measured by immunohistochemistry and Western blotting. Tenascin-x protein expression was negatively correlated with EF, FS, smad-7 and PPARγ, and positively correlated with LVEDd, CVF, smad-3, and TGFβ1 （P 〈0.001）.Conclusion Tenascin-x is an initiator of myocardial fibrosis and ACM development via upregulation of TGFβ1 and downregulation of PPARγ.
文摘Heart failure(HF) following liver transplant(LT) surgery is a distinct clinical entity with high mortality. It is known to occur in absence of obvious risk factors. No preoperative workup including electrocardiogram, echocardiography at rest and on stress, reasonably prognosticates the risk. In patients of chronic liver disease, cirrhotic cardiomyopathy, alcoholic cardiomyopathy, and stress induced cardiomyopathy have each been implicated as a cause for HF after LT. However distinguishing one etiology from another not only is difficult, several etiologies may possibly coexist in a given patient. Diagnostic dilemma is further compounded by the fact that presentation and management of HF irrespective of the possible underlying cause, remains the same. In this case series, 6 cases are presented and in the light of existing literature modification in the preoperative workup are suggested.
基金supported by the National Natural Science Foundation of China(82060312)Yunnan Applied Basic Research Projects(2018FE001(-039),202101AT070249)+1 种基金Fund of Yunnan Province Clinical Research Center for Interventional Medicine(202102AA100067)Graduate Innovation Fund of Kunming Medical University(2021S185).
文摘Alcoholic cardiomyopathy(ACM)is a dilated cardiomyopathy induced by excessive alcohol consumption that causes progressive cardiac dysfunction and chamber dilatation,leading to arrhythmias,heart failure,and death.Cardiac transplantation is the final measure for end-stage ACM.In this case,we used multimodal imaging for ACM diagnosis in a patient with refractory heart failure.The patient received a heart transplant with a good prognosis,and his pathological results after the transplant confirmed our suspected diagnosis.End-stage ACM may lead to refractory heart failure and multimodal imaging may play an important role in the diagnosis,prognosis prediction,and follow-up study of suspected ACM.
