In order to understand the composition and structure of herbicidal active substance from the root of Flaveria bidentis （L.） Kuntze, the isolation and structural identification were researched in this paper. The crud...In order to understand the composition and structure of herbicidal active substance from the root of Flaveria bidentis （L.） Kuntze, the isolation and structural identification were researched in this paper. The crude extract from the root ofF. bidentis （L.） Kuntze was extracted by petroleum ether, ethyl acetate, and water saturation of n-butyl alcohol, respectively, and the extraction fluid was separated by using the method of TLC, then the main fraction was separated by HPLC, and the structure of the herbicidal active substance was analyzed by LC-MS, elemental analysis and ~H-NMR. The results showed that the petroleum extraction had the strongest herbicidal activity, and the purple blue stripe separated by TLC had the strongest effect on Digitaria sanguinalis. The herbicidal active substance was identified as ct-terthienyl according to the data of LC-MS, elemental analysis and 1H-NMR.展开更多
In the present review, we first summarize the main benefits, limitations and pitfalls of conventional in vivo approaches to assessing male reproductive structures and functions in rodents in cases of endocrine active ...In the present review, we first summarize the main benefits, limitations and pitfalls of conventional in vivo approaches to assessing male reproductive structures and functions in rodents in cases of endocrine active substance （EAS） exposure from the postulate that they may provide data that can be extrapolated to humans. Then, we briefly present some integrated approaches in rodents we have recently developed at the organism level. We particularly focus on the possible effects and modes of action （MOA） of these substances at low doses and in mixtures, real-life conditions and at the organ level, deciphering the precise effects and MOA on the fetal testis. It can be considered that the in vivo experimental EAS exposure of rodents remains the first choice for studies and is a necessary tool （together with the epidemiological approach） for understanding the reproductive effects and MOA of EASs, provided the pitfalls and limitations of the rodent models are known and considered. We also provide some evidence that classical rodent models may be refined for studying the multiple consequences of EAS exposure, not only on the reproductive axis but also on various hormonally regulated organs and tissues, among which several are implicated in the complex process of mammalian reproduction. Such models constitute an interesting way of approaching human exposure conditions. Finally, we show that organotypic culture models are powerful complementary tools, especially when focusing on the MOA. All these approaches have contributed in a combinatorial manner to a better understanding of the impact of EAS exposure on human reproduction.展开更多
This study was conducted to identify the biological active substances (BAS) from the fruit of <i>Tetrapleura tetraptera</i> obtained from Ghana. TLC, UV Spectroscopy and differential spectrophotometry meth...This study was conducted to identify the biological active substances (BAS) from the fruit of <i>Tetrapleura tetraptera</i> obtained from Ghana. TLC, UV Spectroscopy and differential spectrophotometry methods of analysis were employed in this study. Identified BAS included flavonoids, polysaccharides and amino acids. Following state pharmacopoeia of Ukraine (SPU) requirements to validate the developed techniques were employed in this study, and the following validation properties specificity, linearity, range, precision accuracy (convergence) and robustness was determined. These characteristics did not exceed the eligibility criteria as stated in (SPU) Appendix 2. Thus the technique can be made to the SPU monograph on fruits of <i>Tetrapleura tetraptera</i>.展开更多
Background: Psychoactive substance use was common among commercial motorcycle operators. Studies had shown that there were some socio-demographic factors which were commoner among commercial motorcycle operators ...Background: Psychoactive substance use was common among commercial motorcycle operators. Studies had shown that there were some socio-demographic factors which were commoner among commercial motorcycle operators who used psychoactive substances. However, this was not explored among commercial motorcycle operators in Kano, North Western Nigeria. Aim: The aim of the study was to determine the socio-demographic factors associated with psychoactive substance use among motorcycle operators in Kano, Northwestern Nigeria. Methods: It was a cross-sectional study. Instrument used for data collection included socio-demographic questionnaire and World Health Organisation Student Drug Use Questionnaire. Data analysis was done using Statistical Package for Social Sciences (SPSS) 16th edition. Results: Commercial motorcycle operators who used substances were found to be of younger age group (X<sup>2</sup> = 10.9, P < 0.001), single (X<sup>2</sup> = 4.3, P = 0.034) and had a father who used tobacco (X<sup>2</sup> = 4.28, P = 0.04). Participants who were single were more likely to use inhalants (X<sup>2</sup> = 21.06, P =0.001), opiates (X<sup>2</sup> = 11.82, P = 0.012), cannabis (X<sup>2</sup> = 5.12, P = 0.028) and stimulants (X<sup>2</sup> = 5.12, P = 0.028) compared to those who were married. Conclusion: This study found that being of younger age, being single, and having a father who smoked were significantly associated with psychoactive substance use. Participants who were singles were more likely to use inhalants, opiates, cannabis and stimulants. No relationship was observed between the age of the respondents and specific substance use. Legislations should be enacted to reduce the rate of psychoactive substance use among motorcycle operators and general population at large.展开更多
Extracellular polymeric substances （EPS） are the predominant constituents of activated sludge and represent up to 80% of the mass of activated sludge. They play a crucial role in the flocculation, settling and dewat...Extracellular polymeric substances （EPS） are the predominant constituents of activated sludge and represent up to 80% of the mass of activated sludge. They play a crucial role in the flocculation, settling and dewatering of activated sludge. Furthermore, EPS also show great efficiency in binding heavy metals. So EPS are key factors influencing reduction in sludge volume and mass, as well as activity and utilization of sludge. EPS are of considerable environmental interest and hundreds of articles on EPS have been published abroad, while information on EPS in China is limited. In this paper, results of over 60 publications related to constituents and characteristics of EPS and their influences on flocculation, settling and dewatering of sludge are compiled and analyzed. Metal-binding ability of EPS is also discussed, together with a brief consideration of possible research interests in the future.展开更多
In the present research, the strain SLYY-3 was isolated from sediments of Jiaozhou Bay, Qingdao, China. The strain SLYY-3, which produced a bacteriocin-like substance(BLS), was characterized to be a strain of Bacillus...In the present research, the strain SLYY-3 was isolated from sediments of Jiaozhou Bay, Qingdao, China. The strain SLYY-3, which produced a bacteriocin-like substance(BLS), was characterized to be a strain of Bacillus subtillis by biochemical profiling and 16 S r DNA sequence analysis. It is the first time to report that Bacillus subtilis from Jiaozhou Bay sediments could produce a BLS. The BLS of B. subtillis SLYY-3 exhibited strong inhibitory activity against gram-positive bacteria(including Staphylococcus aureus and B. subtillis) and some fungi(including Penicillium glaucum, Aspergillus niger and Aspergillus flavus). The antimicrobial activity was detected from culture in the exponential growth phase and reached its maximum when culture entered into stationary growth phase. It was thermo-tolerant even when being kept at 100℃ for 60 min without losing any activity and stable over a wide p H range from 1.0 to 12.0 while being inactivated by proteolytic enzyme and trypsin, indicating the proteinaceous nature of the BLS. The BLS was purified by precipitation with hydrochloric acid(HCl) and gel filteration(Sephadex G-100). SDS-PAGE analysis of the extracellular peptides of SLYY-3 revealed a bacteriocin-like protein with a molecular mass of 66 k Da. Altogether, these characteristics indicate the potential of the BLS for food industry as a protection against pathogenic and spoilage microorganisms.展开更多
Coral mucus covers the surface of coral and contains antibacterial substances as a first line of defense. Coral mucus not only enables the coral itself to resist disease, but also provides antibacterial agents for peo...Coral mucus covers the surface of coral and contains antibacterial substances as a first line of defense. Coral mucus not only enables the coral itself to resist disease, but also provides antibacterial agents for people. We collected mucus from a scleractinian coral （Symphyllia gigantea） at Sta. Sanya （China）, then extracted the antibacterial substances using 10% glacial acetic acid with the help of antiprotease inhibitors, and tested the antibacterial activity by a terrestrial bacterium （Staphylococcus aurevs） and a marine bacterium （Vibrio anguillarum）. The result showed that, there were antibacterial agents in the mucus, and their antibacterial activities were lost by treatment of the sample at 90 ℃ water for 10 min.展开更多
基金the National Natural Science Foundation of China(31171877)
文摘In order to understand the composition and structure of herbicidal active substance from the root of Flaveria bidentis （L.） Kuntze, the isolation and structural identification were researched in this paper. The crude extract from the root ofF. bidentis （L.） Kuntze was extracted by petroleum ether, ethyl acetate, and water saturation of n-butyl alcohol, respectively, and the extraction fluid was separated by using the method of TLC, then the main fraction was separated by HPLC, and the structure of the herbicidal active substance was analyzed by LC-MS, elemental analysis and ~H-NMR. The results showed that the petroleum extraction had the strongest herbicidal activity, and the purple blue stripe separated by TLC had the strongest effect on Digitaria sanguinalis. The herbicidal active substance was identified as ct-terthienyl according to the data of LC-MS, elemental analysis and 1H-NMR.
文摘In the present review, we first summarize the main benefits, limitations and pitfalls of conventional in vivo approaches to assessing male reproductive structures and functions in rodents in cases of endocrine active substance （EAS） exposure from the postulate that they may provide data that can be extrapolated to humans. Then, we briefly present some integrated approaches in rodents we have recently developed at the organism level. We particularly focus on the possible effects and modes of action （MOA） of these substances at low doses and in mixtures, real-life conditions and at the organ level, deciphering the precise effects and MOA on the fetal testis. It can be considered that the in vivo experimental EAS exposure of rodents remains the first choice for studies and is a necessary tool （together with the epidemiological approach） for understanding the reproductive effects and MOA of EASs, provided the pitfalls and limitations of the rodent models are known and considered. We also provide some evidence that classical rodent models may be refined for studying the multiple consequences of EAS exposure, not only on the reproductive axis but also on various hormonally regulated organs and tissues, among which several are implicated in the complex process of mammalian reproduction. Such models constitute an interesting way of approaching human exposure conditions. Finally, we show that organotypic culture models are powerful complementary tools, especially when focusing on the MOA. All these approaches have contributed in a combinatorial manner to a better understanding of the impact of EAS exposure on human reproduction.
文摘This study was conducted to identify the biological active substances (BAS) from the fruit of <i>Tetrapleura tetraptera</i> obtained from Ghana. TLC, UV Spectroscopy and differential spectrophotometry methods of analysis were employed in this study. Identified BAS included flavonoids, polysaccharides and amino acids. Following state pharmacopoeia of Ukraine (SPU) requirements to validate the developed techniques were employed in this study, and the following validation properties specificity, linearity, range, precision accuracy (convergence) and robustness was determined. These characteristics did not exceed the eligibility criteria as stated in (SPU) Appendix 2. Thus the technique can be made to the SPU monograph on fruits of <i>Tetrapleura tetraptera</i>.
文摘Background: Psychoactive substance use was common among commercial motorcycle operators. Studies had shown that there were some socio-demographic factors which were commoner among commercial motorcycle operators who used psychoactive substances. However, this was not explored among commercial motorcycle operators in Kano, North Western Nigeria. Aim: The aim of the study was to determine the socio-demographic factors associated with psychoactive substance use among motorcycle operators in Kano, Northwestern Nigeria. Methods: It was a cross-sectional study. Instrument used for data collection included socio-demographic questionnaire and World Health Organisation Student Drug Use Questionnaire. Data analysis was done using Statistical Package for Social Sciences (SPSS) 16th edition. Results: Commercial motorcycle operators who used substances were found to be of younger age group (X<sup>2</sup> = 10.9, P < 0.001), single (X<sup>2</sup> = 4.3, P = 0.034) and had a father who used tobacco (X<sup>2</sup> = 4.28, P = 0.04). Participants who were single were more likely to use inhalants (X<sup>2</sup> = 21.06, P =0.001), opiates (X<sup>2</sup> = 11.82, P = 0.012), cannabis (X<sup>2</sup> = 5.12, P = 0.028) and stimulants (X<sup>2</sup> = 5.12, P = 0.028) compared to those who were married. Conclusion: This study found that being of younger age, being single, and having a father who smoked were significantly associated with psychoactive substance use. Participants who were singles were more likely to use inhalants, opiates, cannabis and stimulants. No relationship was observed between the age of the respondents and specific substance use. Legislations should be enacted to reduce the rate of psychoactive substance use among motorcycle operators and general population at large.
基金The National Natural Science Foundation of China (No. 50578053) and the Harbin Young Scientist Fund (No. 2003AFXXJ025)
文摘Extracellular polymeric substances （EPS） are the predominant constituents of activated sludge and represent up to 80% of the mass of activated sludge. They play a crucial role in the flocculation, settling and dewatering of activated sludge. Furthermore, EPS also show great efficiency in binding heavy metals. So EPS are key factors influencing reduction in sludge volume and mass, as well as activity and utilization of sludge. EPS are of considerable environmental interest and hundreds of articles on EPS have been published abroad, while information on EPS in China is limited. In this paper, results of over 60 publications related to constituents and characteristics of EPS and their influences on flocculation, settling and dewatering of sludge are compiled and analyzed. Metal-binding ability of EPS is also discussed, together with a brief consideration of possible research interests in the future.
基金supported by the National Science and Technology Support Program (No. 2011BAD14B04)Project of Shandong Province Higher Educational Science and Technology Program (J14LE59)+1 种基金Applied & Basic Research Foundation of Qingdao (No. 12-1-4-3(3)-jch)Science & Technology Project of AQSIQ (No. 2012IK176)
文摘In the present research, the strain SLYY-3 was isolated from sediments of Jiaozhou Bay, Qingdao, China. The strain SLYY-3, which produced a bacteriocin-like substance(BLS), was characterized to be a strain of Bacillus subtillis by biochemical profiling and 16 S r DNA sequence analysis. It is the first time to report that Bacillus subtilis from Jiaozhou Bay sediments could produce a BLS. The BLS of B. subtillis SLYY-3 exhibited strong inhibitory activity against gram-positive bacteria(including Staphylococcus aureus and B. subtillis) and some fungi(including Penicillium glaucum, Aspergillus niger and Aspergillus flavus). The antimicrobial activity was detected from culture in the exponential growth phase and reached its maximum when culture entered into stationary growth phase. It was thermo-tolerant even when being kept at 100℃ for 60 min without losing any activity and stable over a wide p H range from 1.0 to 12.0 while being inactivated by proteolytic enzyme and trypsin, indicating the proteinaceous nature of the BLS. The BLS was purified by precipitation with hydrochloric acid(HCl) and gel filteration(Sephadex G-100). SDS-PAGE analysis of the extracellular peptides of SLYY-3 revealed a bacteriocin-like protein with a molecular mass of 66 k Da. Altogether, these characteristics indicate the potential of the BLS for food industry as a protection against pathogenic and spoilage microorganisms.
基金This work was supported by the National Natural Science Foundation of China under contract No.40576052the Scientific Research Foundation of China Three Gorges University under contract No.0620060113.
文摘Coral mucus covers the surface of coral and contains antibacterial substances as a first line of defense. Coral mucus not only enables the coral itself to resist disease, but also provides antibacterial agents for people. We collected mucus from a scleractinian coral （Symphyllia gigantea） at Sta. Sanya （China）, then extracted the antibacterial substances using 10% glacial acetic acid with the help of antiprotease inhibitors, and tested the antibacterial activity by a terrestrial bacterium （Staphylococcus aurevs） and a marine bacterium （Vibrio anguillarum）. The result showed that, there were antibacterial agents in the mucus, and their antibacterial activities were lost by treatment of the sample at 90 ℃ water for 10 min.
