In this study,a high-throughput screening method was established through the 24-square deep-well microliter plate(MTP) fermentation and micro-plate detection for large-scale screening of the mutants.It was suitable fo...In this study,a high-throughput screening method was established through the 24-square deep-well microliter plate(MTP) fermentation and micro-plate detection for large-scale screening of the mutants.It was suitable for screening a large number of mutants and improving the breeding efficiency after heavy-ion beam irradiation.Seventeen strains showed higher cellulase activity compared with the initial strain after the screening of plate and MTP fermentation.The filter paper activity and β-glucosidase activity of Aspergillus niger H11201 had increased 38.74 and 63.23%separately compared with A.niger H11 by shaking flask fermentation,and it was genetically stable after being passaged to nine generations.The results indicate that the high-throughput screening method can be used for the quick breeding of A.niger with high cellulase activity.展开更多
Abstract [ Objective ] This study aimed to screen a marine-derived cellulase producing filamentous fungus and to investigate the yielding conditions and celluase activity. [ Method] A total of 25 marine-derived filame...Abstract [ Objective ] This study aimed to screen a marine-derived cellulase producing filamentous fungus and to investigate the yielding conditions and celluase activity. [ Method] A total of 25 marine-derived filamentous fungus were screened out of from Bohai Bay, and one producing cellulase was obtained through Congo red transparent circle method and cellulase activity assay. After that, optimum conditions were examined for cellulase producing and catalyzing. [ Result] CMC-Na could induce ZDTJ097-1-（7 ） strain to produce filter paper cellulase, CMC cellulase and 13-glucosidase. The optimum conditions for ZDTJ097-1-（7） to produce en- zymes were 0.5% CMC-Na, 0. 2% NH4NO3 , pH 6.0 at 25 ℃. [ Conclusion] This study provided reference for furfller development and utilization of cellulase produ-cing fungus ZDTJ097-1-（7）.展开更多
The cellulase was used for extracting the volatile constituents of the Leonurus japonicus Houtt.The effects of the pH,the amounts and the fineness of cellulase were discussed.The average yield obtained was 4.05%.16 ch...The cellulase was used for extracting the volatile constituents of the Leonurus japonicus Houtt.The effects of the pH,the amounts and the fineness of cellulase were discussed.The average yield obtained was 4.05%.16 chemical components were identified by gas chromatography-mass spectrometry (GC-MS)method.The cellulase extraction (CE)method was made a camparison with distillation extraction (DSE)method.展开更多
基金supported by the National Natural Science Foundation of China(No.11305225)the Science and Technology Service Network Initiative(KFJ-EW-STS-086)
文摘In this study,a high-throughput screening method was established through the 24-square deep-well microliter plate(MTP) fermentation and micro-plate detection for large-scale screening of the mutants.It was suitable for screening a large number of mutants and improving the breeding efficiency after heavy-ion beam irradiation.Seventeen strains showed higher cellulase activity compared with the initial strain after the screening of plate and MTP fermentation.The filter paper activity and β-glucosidase activity of Aspergillus niger H11201 had increased 38.74 and 63.23%separately compared with A.niger H11 by shaking flask fermentation,and it was genetically stable after being passaged to nine generations.The results indicate that the high-throughput screening method can be used for the quick breeding of A.niger with high cellulase activity.
基金Supported by the National Natural Science Foundation of China(30971904)
文摘Abstract [ Objective ] This study aimed to screen a marine-derived cellulase producing filamentous fungus and to investigate the yielding conditions and celluase activity. [ Method] A total of 25 marine-derived filamentous fungus were screened out of from Bohai Bay, and one producing cellulase was obtained through Congo red transparent circle method and cellulase activity assay. After that, optimum conditions were examined for cellulase producing and catalyzing. [ Result] CMC-Na could induce ZDTJ097-1-（7 ） strain to produce filter paper cellulase, CMC cellulase and 13-glucosidase. The optimum conditions for ZDTJ097-1-（7） to produce en- zymes were 0.5% CMC-Na, 0. 2% NH4NO3 , pH 6.0 at 25 ℃. [ Conclusion] This study provided reference for furfller development and utilization of cellulase produ-cing fungus ZDTJ097-1-（7）.
文摘The cellulase was used for extracting the volatile constituents of the Leonurus japonicus Houtt.The effects of the pH,the amounts and the fineness of cellulase were discussed.The average yield obtained was 4.05%.16 chemical components were identified by gas chromatography-mass spectrometry (GC-MS)method.The cellulase extraction (CE)method was made a camparison with distillation extraction (DSE)method.
