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Effect of dissolved phosphorus on alkaline phosphatase activity in marine microalgae 被引量:2
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作者 Huang Bangqin1,2 Huang Shiyu3 +1 位作者 Weng Yan2 Hong Huasheng1,2 (1. Research Laboratory of SEDC on Marine Ecological Environment, Xiamen University, Xiamen 361005, China 2. Environmental Science Research Center, Xiamen University, Xiamen 361005, China 3. Fis 《Acta Oceanologica Sinica》 SCIE CAS CSCD 2000年第2期29-35,共7页
Alkaline phosphatase activity (APA) and dissolved phosphorus were monitored during the batch cultures of two bone microalgae. Results indicate that variation of APA was in the shape of 'S' curve. Different spe... Alkaline phosphatase activity (APA) and dissolved phosphorus were monitored during the batch cultures of two bone microalgae. Results indicate that variation of APA was in the shape of 'S' curve. Different specs of dissolved phaphorus had different effects on APA. The concentrations of dis solved inorganic phosphorus (DIP) and and molecular dissolved organic phosphorus (SDOP) had a sig nificant effect on APA, while the concentration of large molecular dissolved organic phosphorus (LDOP) had a little effect on APA., and the increase of APA could accelerate the decomposing of LDOP in the medium. Results also show that algae species and abundance had why a little effect on APA. 展开更多
关键词 alkaline phosphatase activity dissolved inorganic phosphorus small molecular dissolved or ganic phophorus large molecular dissolved organic phosphorus MICROALGAE
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Development of a Detection Method for Assaying Calf Intestine Alkaline Phosphatase Activity
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作者 Lin Lin Lai Xiaofang +4 位作者 Li Yi Liu Yixiong Lan Quanxue Lai Xintian Yang Guowu 《Animal Husbandry and Feed Science》 CAS 2015年第3期152-155,166,共5页
[ Objective] This study was to develop a detection method for assaying calf intestine alkaline phasphatase activity in scientific research. [ Method ] By simulating the conditions and buffers for assaying calf intesti... [ Objective] This study was to develop a detection method for assaying calf intestine alkaline phasphatase activity in scientific research. [ Method ] By simulating the conditions and buffers for assaying calf intestine alkaline phosphatase used in the scientific research, the parameters influencing substmte concentration, reaction duration, eoloration time and buffer pH were optimized. [ Result] The activity of alkaline phosphatase detected varied hugely among different buffers, and potassium ferricyanide solution added with boracic acid achieved the stable coloration. The optimal water bath time was determined as 10 rain, and the substrate concentration was optimized as 0.04 moL/L. The increasing temperature did not have a large influence on low temperature enzymatic activity while did on high coneentration enzymatic activity. When the buffer pH was 7.0 - 8.0, the detection stability of alkaline phosphatase could be maintained well The ion intensity of buff- er had little effects on alkaline phasphatase activity. [ Conclusion] A detection method for assaying calf intestine alkaline phosphatase activity in scientific research was successfully developed in this study. 展开更多
关键词 alkaline phosphatase Calf intestine activity detection Ultraviolet spectrophotomctry Scientific research
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Influence of sodium fluoride on alkaline phosphatase activity and bone gla protein synthesis in human yellow ligament cells in vitro
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作者 王哲 《外科研究与新技术》 2011年第2期88-88,共1页
Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human... Objective To investigate the influence of sodium fluoride(NaF)on alkaline phosphatase(ALP)activity and bone gla protein(BGP)synthesis in yellow ligament cells from different surgical simples in vitro.Methods The human ligament cells 展开更多
关键词 bone Influence of sodium fluoride on alkaline phosphatase activity and bone gla protein synthesis in human yellow ligament cells in vitro NaF
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Prussian Blue Nanoparticles with High Oxidase-like Activity for Simultaneous Detection of Ascorbic Acid and Alkaline Phosphatase in Real Samples
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作者 TANG Zeng LAI Yue +4 位作者 LU Hao XIA Yi JIANG Xue XIONG Xiaoli XIAO Ting 《Chemical Research in Chinese Universities》 SCIE CAS CSCD 2024年第6期1023-1032,共10页
Prussian blue nanoparticles (PB NPs) have exhibited favorable anti-inflammatory effects in animal models,and have been proved to mimic three antioxidant enzymes: peroxidase,catalase,and superoxide dismutase.Whereas th... Prussian blue nanoparticles (PB NPs) have exhibited favorable anti-inflammatory effects in animal models,and have been proved to mimic three antioxidant enzymes: peroxidase,catalase,and superoxide dismutase.Whereas the oxidase-like activity of PB NPs has not been demonstrated.In this work,PB NPs with outstanding oxidase-like activity were demonstrated by virtue of the colorimetric substrate o-phenylenediamine (OPD).Interestingly,based on the oxidase-like activity,PB NPs can also catalytically oxidize vitamin B1 (VB1) to strongly fluorescent product in the physiological environment without the addition of other oxidants.Considering that VB1 has no toxicity and carcinogenicity comparing with OPD,a novel PB NPs-VB1 fluorescent sensor was further developed for simple,nontoxic,and rapid detection of ascorbic acid (AA) in foods and alkaline phosphatase (ALP) in human serum samples by taking advantage of the reduction of PB NPs to Prussian White by AA or enzymatic hydrolysate of ALP and ascorbic acid 2-phosphate (AA2P);leading to the decrease of fluorescence intensity.The extremely low detection limits of 44 nmol/L for AA and 72 mU/L for ALP as well as the excellent applicability in real samples were both obtained.Therefore,we envision that such a novel fluorescent probe would be potentially applied in the food analysis and clinic diagnosis fields. 展开更多
关键词 Prussian blue nanoparticles Oxidase-like activity Fluorescence probe Ascorbic acid alkaline phosphatase
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Responses of alkaline phosphatase activity to wind-driven waves in a large,shallow lake:Implications for phosphorus availability and algal blooms 被引量:5
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作者 Jian Zhou Xiaoxia Han +1 位作者 Boqiang Qin Guangwei Zhu 《Journal of Environmental Sciences》 SCIE EI CAS CSCD 2021年第1期143-150,共8页
Phosphorus is a vital nutrient for algal growth,thus,a better understanding of phosphorus availability is essential to mitigate harmful algal blooms in lakes.Wind waves are a ubiquitous characteristic of lake ecosyste... Phosphorus is a vital nutrient for algal growth,thus,a better understanding of phosphorus availability is essential to mitigate harmful algal blooms in lakes.Wind waves are a ubiquitous characteristic of lake ecosystems.However,its effects on the cycling of organic phosphorus and its usage by phytoplankton remain poorly elucidated in shallow eutrophic lakes.A mesocosm experiment was carried out to investigate the responses of alkaline phosphatase activity fractions to wind waves in large,shallow,eutrophic Lake Taihu.Results showed that wind-driven waves induced the release of alkaline phosphatase and phosphorus from the sediment,and dramatically enhanced phytoplanktonic alkaline phosphatase activity.However,compared to the calm conditions,bacterial and dissolved alkaline phosphatase activity decreased in wind-wave conditions.Consistently,the gene copies of Microcystis phoX increased but bacterial phoX decreased under wind-wave conditions.The ecological effects of these waves on phosphorus and phytoplankton likely accelerated the biogeochemical cycling of phosphorus and promoted phytoplankton production in Lake Taihu.This study provides an improved current understanding of phosphorus availability and the phosphorus strategies of plankton in shallow,eutrophic lakes. 展开更多
关键词 Wind waves alkaline phosphatase activity PHOSPHORUS PLANKTON PHOX
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Effects of simulated microgravity on the alkaline phosphatase activity and intracellular calcium concentration of cultured chondrocytes
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作者 Xiaobing Li Shuzhang Yang +2 位作者 Shengguang Li Peidong Jiang Zhihuan Lin 《Chinese Science Bulletin》 SCIE EI CAS 1999年第3期218-221,共4页
The effects of simulated microgravity on matrix mineralization of chondrocytes were examined using cultured chicken embryonic chondrocytes as the model. In four days, there was a time course decrease in alkaline phosp... The effects of simulated microgravity on matrix mineralization of chondrocytes were examined using cultured chicken embryonic chondrocytes as the model. In four days, there was a time course decrease in alkaline phosphatase activity of chondrocytes, a marker of matrix mineralization.Meanwhile, in two days, there was a significant drop in intracellular calcium concentration in contrast to the control. These results indicate that simulated microgravity can suppress matrix calcification of cultured chondrocytes, and intracellular calcium may be involved in the regulation of matrix calcification as the second messenger. 展开更多
关键词 CHONDROCYTE CALCIFICATION simulated MICROGRAVITY alkaline phosphatase activity INTRACELLULAR calcium concentration.
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Activation of Calf Intestinal Alkaline Phosphatase by Trifluoroethanol 被引量:2
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作者 曹志方 徐真 +1 位作者 朴龙斗 周海梦 《Tsinghua Science and Technology》 SCIE EI CAS 2001年第5期426-431,457,共7页
Alkaline phosphatase is a stable enzyme which is strongly resistant to urea, guanidine hydrochloride, acid pH, and heat. But there have been few studies on the effect of organic cosolvents on the activity and struct... Alkaline phosphatase is a stable enzyme which is strongly resistant to urea, guanidine hydrochloride, acid pH, and heat. But there have been few studies on the effect of organic cosolvents on the activity and structure of alkaline phosphatase. The activity of calf intestinal alkaline phosphatase (CIAP) is markedly increased when incubated in solutions with elevated trifluoroethanol (TFE) concentrations. The activation is a time dependent course. There is a very fast phase in the activation kinetics in the mixing dead time (30 s) using convential methods. Further activation after the very fast phase follows biphasic kinetics. The structural basis of the activation has been monitored by intrinsic fluorescence and far ultraviolet circular dichroism. TFE (060%) did not lead to any significant change in the intrinsic fluorescence emission maximum, indicating no significant change in the tertiary structure of CIAP. But TFE did significantly change the secondary structure of CIAP, especially increasing α helix content. We conclude that the activation of CIAP is due to its secondary structural change. The time for the secondary structure change induced by TFE preceds that of the activity increase. These results suggest that a rapid conformational change of CIAP induced by TFE results in the enhancement of CIAP activity, followed by further increase of this activity because of the further slightly slower rearrangements of the activated conformation. It is concluded that the higher catalytic activity of CIAP can be attained with various secondary structures. 展开更多
关键词 alkaline phosphatase TRIFLUOROETHANOL activATION CONFORMATION
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Activation of p38 mitogen-activated protein kinase contribute to BMP4-induced alkaline phosphatase expression in MC3T3-E1 preosteoblast 被引量:9
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作者 YUAN Ye Wu Zhi-jun +5 位作者 YAO Hui-yu YU Xiao-dan GUO Zi-kuan CHEN Xiao-san TANG Pei-xian MAO Ning 《Chinese Medical Journal》 SCIE CAS CSCD 2006年第4期324-327,共4页
Bone morphogenetic proteins (BMPs) induce ectopic bone formation and promote osteoblast differentiation. It has been documented that Smad transcriptional factors function as primary mediators of BMPs activity. Recep... Bone morphogenetic proteins (BMPs) induce ectopic bone formation and promote osteoblast differentiation. It has been documented that Smad transcriptional factors function as primary mediators of BMPs activity. Receptor-regulated Smad (Smad1, 5, 8) could be phosphorylated by activated BMPR-I and form complex with Smad4. The Smad complex translocates to the nucleus and regulate target gene transcription. Recently, several reports suggested that Mitogen-Activated Protein Kinase (MAPK) signaling pathways could be initiated downstream of the BMP receptor complex. Alkaline phosphatase (ALP) is an early marker of osteoblast differentiation Both ALP activity and its mRNA expression level could be increased by BMP4 treatment. Previously, we demonstrated that mutation of ERK1/2 phosphorylation sites in Smad5 partially rescued Smad transcriptional activity. However, fibroblast growth factor2-suppressed ALP activity could not be rescued similarly by introduction of Smad5 mutant in MC3T3-E1. These results prompted us to further evaluate the effect of BMP4-stimulated Smad transcriptional activity on ALP expression in this study. 展开更多
关键词 bone morphogenetic protein4 p38 mitogen-activated protein kinase alkaline phosphatase
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骨碎补UPLC指纹图谱与人骨肉瘤细胞MG63中碱性磷酸酶活性的谱效关系研究
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作者 陈香丽 张慧敏 张紫佳 《天然产物研究与开发》 CAS CSCD 北大核心 2024年第2期293-302,共10页
建立骨碎补生品和砂烫品提取物的UPLC指纹图谱,并研究骨碎补提取物与人骨肉瘤细胞MG63中碱性磷酸酶(ALP)活性之间的谱效关系。采用UPLC法建立骨碎补生品和烫品的指纹图谱并通过UPLC-Q-TOF MS法对其化学成分进行鉴别;通过测定MG63细胞中... 建立骨碎补生品和砂烫品提取物的UPLC指纹图谱,并研究骨碎补提取物与人骨肉瘤细胞MG63中碱性磷酸酶(ALP)活性之间的谱效关系。采用UPLC法建立骨碎补生品和烫品的指纹图谱并通过UPLC-Q-TOF MS法对其化学成分进行鉴别;通过测定MG63细胞中ALP活性检测骨碎补提取物对MG63细胞成骨分化的影响;采用灰色关联度分析、双变量相关性分析以及偏最小二乘回归分析法分析骨碎补生品和烫品的UPLC特征指纹峰与其促ALP活性之间的谱效关系。在标定的12个共有峰中,确定了4、5、12号峰与ALP活性呈正相关,其对应的物质依次是咖啡酸-4-O-β-D-吡喃葡萄糖苷、儿茶素7-葡糖苷、柚皮苷,为深入研究骨碎补的促成骨分化活性物质基础提供依据。 展开更多
关键词 骨碎补 UPLC指纹图谱 碱性磷酸酶活性 谱效关系 人骨肉瘤细胞MG63
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鲍鱼碱性磷酸酶的分离纯化和性质研究 被引量:46
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作者 廖金花 陈巧 +2 位作者 林丽蓉 洪燕飞 陈清西 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2005年第2期272-275,共4页
以杂色鲍(Haliotis diversicolor)内脏为原料,经Tris HCl缓冲液(pH 7.5,含0.1mol/L NaCl)抽提,正丁醇处理,硫酸铵分级沉淀分离,DE 32离子交换、Sephadex G 150分子筛、DE 32纯化,得到电泳单一纯的碱性磷酸酶制剂,比活力为1 226 U/mg.酶... 以杂色鲍(Haliotis diversicolor)内脏为原料,经Tris HCl缓冲液(pH 7.5,含0.1mol/L NaCl)抽提,正丁醇处理,硫酸铵分级沉淀分离,DE 32离子交换、Sephadex G 150分子筛、DE 32纯化,得到电泳单一纯的碱性磷酸酶制剂,比活力为1 226 U/mg.酶学性质和动力学性质研究表明:该酶催化对硝基苯磷酸水解反应的最适 pH值为 10.08,最适温度为48℃.米氏常数Km 值为0.80 mmol/L,Vm 值为20.1 mmol/L·min-1(pH 10.0,37℃).酶的热稳定性研究表明:该酶在pH 7~11区间和在温度低于55℃下稳定.金属离子对酶活力的影响结果表明:Li+、Na+和K+对酶活力没有影响,Mg2+、Ca2+、Ba2+、Mn2+和Co2+对酶有不同程度的激活作用,而Cu2+、Cd2+、Zn2+、Hg2+、Ag+、Bi2+和Pb2+等对酶起抑制作用. 展开更多
关键词 杂色鲍 碱性磷酸酶 分离纯化 酶活性 稳定性 金属离子 鲍鱼 生物体
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环境因子对湖泊沉积物碱性磷酸酶活性的影响 被引量:11
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作者 张翠英 徐德兰 +2 位作者 万蕾 琚淑明 高明侠 《环境科学与技术》 CAS CSCD 北大核心 2013年第4期23-27,共5页
文章对云龙湖和骆马湖2种不同类型湖泊沉积物的碱性磷酸酶进行了测定,分析了水体理化特性、季节变化及水生植物对湖泊沉积物碱性磷酸酶活性的影响,结果表明:pH值、温度对沉积物碱性磷酸酶活性影响较大,呈显著正相关(r=0.220,P70.05;r=0.... 文章对云龙湖和骆马湖2种不同类型湖泊沉积物的碱性磷酸酶进行了测定,分析了水体理化特性、季节变化及水生植物对湖泊沉积物碱性磷酸酶活性的影响,结果表明:pH值、温度对沉积物碱性磷酸酶活性影响较大,呈显著正相关(r=0.220,P70.05;r=0.478,P70.01),而DO值的影响较小,相关不显著;随着季节的变化,云龙湖沉积物碱性磷酸酶活性变化较明显,其变化规律为夏季>秋季>冬季>春季,夏季最高,为330.27 mg/(kg·h),春季最低,为154.65 mg/(kg·h);随着季节的变化,水体pH、DO变化较小,分别为8.12~8.50、6.84~8.47 mg/L,温度变化较大,为3.3~26.5℃;水生植物对骆马湖沉积物碱性磷酸酶活性影响较大,有草区均大于无草区且有草区碱性磷酸酶活性随季节变化显著。因此,水体理化特性、季节变化及水生植物等环境因子对不同湖泊沉积物中碱性磷酸酶活性有重要影响。 展开更多
关键词 碱性磷酸酶活性 环境因子 云龙湖 骆马湖
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太湖梅梁湾中碱性磷酸酶的活性及其与藻类生长的关系 被引量:51
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作者 高光 秦伯强 +2 位作者 朱广伟 范成新 季江 《湖泊科学》 EI CAS CSCD 2004年第3期245-251,共7页
通过对1998年5月-1999年5月的太湖梅梁湾水体中碱性磷酸酶活性及其它水化学因子的同步实地监测,初步探讨了富营养化较严重的太湖梅梁湾湖区的碱性磷酸酶活性的时空变化规律及其与藻类水华的相关性.研究表明,水体中各种形态磷之间的转化... 通过对1998年5月-1999年5月的太湖梅梁湾水体中碱性磷酸酶活性及其它水化学因子的同步实地监测,初步探讨了富营养化较严重的太湖梅梁湾湖区的碱性磷酸酶活性的时空变化规律及其与藻类水华的相关性.研究表明,水体中各种形态磷之间的转化非常快.在磷的循环、转化过程中,碱性磷酸酶的作用至关重要.太湖梅梁湾各采样点水体中碱性磷酸酶的最大反应速率(Vmax)的年际变化有着显著的规律性,各点位在春季(3-4月)及夏季(7-8月)均分别出现峰值,与水体中水华出现的峰值相吻合.尤其在水体中水华暴发前的4月份,各采样点中的碱性磷酸酶的活性急剧增加,其Vmax均为年内的最大值或接近最大值,预示着水体中其它形态磷的转化速率加快,为水华的形成提供了充足的活性磷.水体中特异性碱性磷酸酶活性(总碱性磷酸酶活性/Chl.a)与水体中的PO43-存在着较好的负相关.尤其是在春季相关性更加显著,可达-0.9以上;夏季太湖梅梁湾水华暴发时,水体中的磷酸盐浓度远低于碱性磷酸酶的激发阈值,藻类体中的酶被诱导大量产生,从而使得水体中碱性磷酸酶的数量、活性急剧增加,达到较高的水平.这种短时间的有机质快速降解以及由此导致的营养盐释放,维持了水体中藻类的生长. 展开更多
关键词 太湖 碱性磷酸酶 活性 藻类 动力学参数 磷循环
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滇池福保湾间隙水氮磷分布及其与底泥微生物和磷酸酶相互关系 被引量:26
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作者 李宝 丁士明 +4 位作者 范成新 钟继承 张路 尹洪斌 赵斌 《湖泊科学》 CAS CSCD 北大核心 2008年第4期420-427,共8页
在滇池福保湾不同区域应用Peeper(渗析膜式)技术,分析了底泥间隙水NH4+-N、PO43--P的垂向分布特征和近表层10cm内底泥的微生物活性(FDA)、碱性磷酸酶活性(APA),并对它们之间的相互关系进行了统计分析.结果表明,NH4+-N和PO43--P浓度自上... 在滇池福保湾不同区域应用Peeper(渗析膜式)技术,分析了底泥间隙水NH4+-N、PO43--P的垂向分布特征和近表层10cm内底泥的微生物活性(FDA)、碱性磷酸酶活性(APA),并对它们之间的相互关系进行了统计分析.结果表明,NH4+-N和PO43--P浓度自上覆水向下层间隙水呈先升后降趋势,反映它们有自间隙水向上覆水扩散的潜在危害;底泥有机质(Loss-on-Ignition,LOI)、APA和FDA活性也有从表层底泥向下层逐步降低的趋势.在空间分布上,NH4+-N、PO43--P浓度变化为河口区>湾心区>西部沿岸区>东部沿岸区,与沉积物中LOI、APA和FDA活性的大小顺序基本相同.间隙水NH4+-N浓度与表层10cm内底泥的APA和FDA活性具有显著正相关性(α=0.01),PO43--P浓度与底泥APA和FDA活性具有负相关性,但相关系数很低. 展开更多
关键词 Peeper 营养盐 沉积物 微生物活性 碱性磷酸酶 滇池
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浅层湖泊沉积物碱性磷酸酶活性垂向特征初探 被引量:8
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作者 苏玉萍 郑达贤 +1 位作者 曾花森 林婉珍 《福建师范大学学报(自然科学版)》 CAS CSCD 北大核心 2005年第3期35-38,共4页
研究了福州市西湖沉积物中碱性磷酸酶活性(PAP)的垂向分布特征,探讨了其活性的来源及影响因素.初步的研究表明:西湖沉积物中的碱性磷酸酶活性和底泥中溶解性磷的变化趋势基本一致,但高浓度的溶解性磷对APA产生一定的抑制作用;活性的来... 研究了福州市西湖沉积物中碱性磷酸酶活性(PAP)的垂向分布特征,探讨了其活性的来源及影响因素.初步的研究表明:西湖沉积物中的碱性磷酸酶活性和底泥中溶解性磷的变化趋势基本一致,但高浓度的溶解性磷对APA产生一定的抑制作用;活性的来源与水体中沉积下来生物的残体以及微生物代谢物有关. 展开更多
关键词 碱性磷酸酶活性 沉积物 垂向 西湖
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太湖水体中碱性磷酸酶的作用阈值 被引量:31
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作者 高光 高锡芸 +1 位作者 秦伯强 季江 《湖泊科学》 EI CAS CSCD 2000年第4期353-358,共6页
对不同PO3- 4 P浓度下 ,不同粒级组分中碱性磷酸酶活性的分布及其变动规律的研究表明 :①随着水体中PO3- 4 P浓度的增加 ,水体中总碱性磷酸酶活性 (TAPA)相应地减少 (r =- 0 .586,n =55) .水体中的TAPA均值 ,在PO3- 4 P浓度范围为 0 - 0... 对不同PO3- 4 P浓度下 ,不同粒级组分中碱性磷酸酶活性的分布及其变动规律的研究表明 :①随着水体中PO3- 4 P浓度的增加 ,水体中总碱性磷酸酶活性 (TAPA)相应地减少 (r =- 0 .586,n =55) .水体中的TAPA均值 ,在PO3- 4 P浓度范围为 0 - 0 .0 10mg·L- 1,无显著差异 ;而当PO3- 4 P浓度 >0 .0 10mg·L- 1时 ,差异极显著 (P <0 .0 1) .②水体中的碱性磷酸酶在不同粒级组分中的分布差异明显 ,其中以溶解性碱性磷酸酶活性 (DAPA)所占的比例最大 ,浮游植物体中的碱性磷酸酶活性 (PAPA)所占的比例次之 ,细菌体中的碱性磷酸酶活性 (BAPA)所占的比例最小 .③随着水体中的PO3- 4 P浓度的增加 ,水体中的PAPA也相应地减少 .水体中的PAPA的活性均值 ,在PO3- 4 P浓度范围为 0 - 0 .0 2 0mg·L- 1时 ,无显著差异 ;而当PO3- 4 P浓度 >0 .0 2 0mg·L- 1时 ,差异显著 (P <0 .0 5) ;当PO3- 4 P浓度 >0 .2 0 0mg·L- 1,差异极显著 (P <0 .0 1) .④水体中的BAPA未表现出与PO3- 4 P浓度的相关性 . 展开更多
关键词 太湖 碱性磷酸酶 阈值 生物代谢 有机磷 实验
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合浦珠母贝碱性磷酸酶的分离纯化与性质研究 被引量:25
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作者 谢莉萍 林静瑜 +1 位作者 肖锐 张荣庆 《海洋科学》 CAS CSCD 北大核心 2000年第10期37-40,共4页
以合浦珠母贝 (Pinctadafucata)为提取酶的原料 ,经Tris HCl缓冲液 (pH7.5)抽提 ,正丁醇处理 ,硫酸铵分级沉淀分离 ,DEAE 纤维素离子交换树脂柱层析 ,DEAE A25离子交换分子筛柱层析纯化 ,得到一定纯度的碱性磷酸酶 ,比活力达到1444U/mg... 以合浦珠母贝 (Pinctadafucata)为提取酶的原料 ,经Tris HCl缓冲液 (pH7.5)抽提 ,正丁醇处理 ,硫酸铵分级沉淀分离 ,DEAE 纤维素离子交换树脂柱层析 ,DEAE A25离子交换分子筛柱层析纯化 ,得到一定纯度的碱性磷酸酶 ,比活力达到1444U/mg。酶学性质和动力学性质研究表明 ,该酶催化对硝基苯磷酸 (pNPP)的水解反应 ,最适 pH值为9.72,最适温度为45℃ ,水解反应的活化能为Ka=21.4kJ/mol,初速度为6.1μmol/L,米氏常数Km 值为2.86mmol/L,Vm 值为9.09μmol/L。产物类似物HPO2 - 4,WO3 - 4 对该酶均有明显的抑制作用 ,表现为竞争性抑制。金属离子对该酶的催化活力有不同的影响 ,正一价金属离子对该酶活性无影响 ,Mg2 +,Co2 +,Mn2 +对该酶有激活作用 ,Zn2 +对该酶有明显的抑制作用。 展开更多
关键词 合浦珠母贝 碱性磷酸酶 酶活性 分离 人工育珠
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草鱼碱性磷酸酶的分离纯化与部分性质研究 被引量:21
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作者 张继平 林建成 +1 位作者 谢进金 陈清西 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2005年第5期684-687,共4页
用Tris-HCl缓冲液抽提,硫酸铵分级沉淀,DEAE-纤维素和葡聚糖凝胶层析,从草鱼肠中提取出碱性磷酸酶(ALP).提取倍数为6.74,比活为684 U/mg蛋白.酶液经PAGE呈现单一带,该酶催化对硝基苯磷酸二钠(pNPP)水解反应的最适pH值为10.41,最适温度... 用Tris-HCl缓冲液抽提,硫酸铵分级沉淀,DEAE-纤维素和葡聚糖凝胶层析,从草鱼肠中提取出碱性磷酸酶(ALP).提取倍数为6.74,比活为684 U/mg蛋白.酶液经PAGE呈现单一带,该酶催化对硝基苯磷酸二钠(pNPP)水解反应的最适pH值为10.41,最适温度为27℃,Km值为1.01 mmol/L,酶的热稳定性与pH稳定性研究表明:该酶在pH 6.6~11.5区间和在温度低于45℃下稳定.研究金属离子对酶活力影响的结果表明:一价金属离子Li+、Na+和K+对酶活力没有影响;二价金属离子Mg2+、Mn2+和Co2+对酶有不同程度的激活作用;Zn2+对酶有抑制作用. 展开更多
关键词 草鱼 碱性磷酸酶 分离纯化 温度 PH 金属离子
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生物炭对土壤酶活性和糜子产量的影响 被引量:31
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作者 尚杰 耿增超 +4 位作者 陈心想 张雯 赵军 王森 王亚萍 《干旱地区农业研究》 CSCD 北大核心 2015年第2期146-151,158,共7页
为探讨生物炭对土壤主要酶活性和糜子产量的影响,采用糜子盆栽种植方式,分别在砂土、壤土和盐土三种土壤上设置了生物炭施用量为B0(0 t·hm-2)、B15(15 t·hm-2)、B30(30 t·hm-2)、B45(45 t·hm-2)和B60(60 t·hm... 为探讨生物炭对土壤主要酶活性和糜子产量的影响,采用糜子盆栽种植方式,分别在砂土、壤土和盐土三种土壤上设置了生物炭施用量为B0(0 t·hm-2)、B15(15 t·hm-2)、B30(30 t·hm-2)、B45(45 t·hm-2)和B60(60 t·hm-2)五个水平。结果表明:砂土、壤土和盐土这三种土壤分别对应生物炭用量为45 t·hm-2、45 t·hm-2和30 t·hm-2时土壤总体酶活性(Et)达到最高,并且盐土、砂土和壤土的总体酶活性指标最大值分别比相应对照B0增加16.59%、7.29%、4.07%,说明生物炭对盐土酶活性的影响显著高于砂土,砂土高于壤土;在砂土中施用生物炭后,对糜子有明显的增产效果,而壤土和盐土上增产效果不显著;生物炭的施用还促进了砂土中糜子生物量的积累,并且在壤土上糜子的生物量的积累高于盐土和砂土。总之,在土壤中施用生物炭,不仅可以促进糜子增产,增加土壤总体酶活性,还可以改良土壤生物学特性。 展开更多
关键词 生物炭 蔗糖酶 过氧化氢酶 脲酶 碱性磷酸酶 总体酶活性指标 糜子产量
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海水中碱性磷酸酶活力的测定及其在磷的循环中的作用初探 被引量:55
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作者 洪华生 戴民汉 郑效成 《海洋与湖沼》 CAS CSCD 北大核心 1992年第4期415-420,共6页
建立了海水介质中浮游植物、细菌及游离态三种碱性磷酸酶活力的分光测定法。在厦门西港海域及实验生态中分别测定了这三种碱性磷酸酶的活力;同时采用^(32)P示踪法探讨了碱性磷酸酶对小球藻直接利用溶解有机磷(DOP)的影响。结果表明,浮... 建立了海水介质中浮游植物、细菌及游离态三种碱性磷酸酶活力的分光测定法。在厦门西港海域及实验生态中分别测定了这三种碱性磷酸酶的活力;同时采用^(32)P示踪法探讨了碱性磷酸酶对小球藻直接利用溶解有机磷(DOP)的影响。结果表明,浮游植物体的碱性磷酸酶活力占主导地位;当水体溶解无机磷降低到一定浓度时,浮游植物体碱性磷酸酶活力可突增,它提供了浮游植物直接利用DOP的条件。 展开更多
关键词 海水 磷酸酶 碱性 活力
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低磷胁迫对落叶松幼苗生长及根系酸性磷酸酶活性的影响 被引量:28
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作者 陈永亮 李修岭 周晓燕 《北京林业大学学报》 CAS CSCD 北大核心 2006年第6期46-50,共5页
以磷充足的完全营养液为对照,研究了低磷胁迫对落叶松幼苗生长、磷吸收及根系酸性磷酸酶活性(APA)的影响.结果表明:低磷胁迫下幼苗的平均单株干重净增量较对照显著降低(P〈0.05),仅为对照的20.5%-26.3%.而苗木的根冠比(R/... 以磷充足的完全营养液为对照,研究了低磷胁迫对落叶松幼苗生长、磷吸收及根系酸性磷酸酶活性(APA)的影响.结果表明:低磷胁迫下幼苗的平均单株干重净增量较对照显著降低(P〈0.05),仅为对照的20.5%-26.3%.而苗木的根冠比(R/S)较对照明显增加,可能是落叶松幼苗在低磷胁迫时为增加磷吸收而在形态上主动采取的一种适应性机制.低磷胁迫下苗木叶磷浓度与单株苗木磷含量均较对照显著降低(P〈0.05),单株苗木磷含量为对照的35.38%~54.25%.低磷胁迫下,幼苗根组织内酸性磷酸酶活性与根系分泌的酸性磷酸酶活性均表现出增加,分别为对照的1.06~1.32倍和1.81~2.10倍.根系分泌的酸性磷酸酶活性的增高。 展开更多
关键词 低磷胁迫 生长 磷含量 酸性磷酸酶活性 落叶松
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