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circMAN1A2调节miR-212-3p/DDIT4轴对胃癌细胞增殖、侵袭和迁移的影响
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作者 曹博威 贾腾飞 +2 位作者 宋钉町 赵昱清 张文斌 《胃肠病学和肝病学杂志》 CAS 2024年第7期868-874,共7页
目的探究circMAN1A2靶向miR-212-3p/DNA损伤诱导转录子4(NDA damage-inducible transcript 4,DDIT4)轴对胃癌细胞增殖、侵袭和迁移的影响。方法qRT-PCR法检测胃癌组织和细胞中circMAN1A2、miR-212-3p表达水平。荧光素酶检测circMAN1A2与... 目的探究circMAN1A2靶向miR-212-3p/DNA损伤诱导转录子4(NDA damage-inducible transcript 4,DDIT4)轴对胃癌细胞增殖、侵袭和迁移的影响。方法qRT-PCR法检测胃癌组织和细胞中circMAN1A2、miR-212-3p表达水平。荧光素酶检测circMAN1A2与miR-212-3p、DDIT4和miR-212-3p的靶向关系。在胃癌细胞SGC-7901、HGC-27中转染si-NC、si-circMAN1A2、si-circMAN1A2+anti-NC、si-circMAN1A2+anti-miR-212-3p、miR-NC、miR-212-3p mimics,将未转染的SGC-7901、HGC-27细胞设为对照。平板克隆实验检测细胞增殖;细胞划痕及Transwell实验分别检测细胞迁移、侵袭能力。Western blotting检测增殖、迁移及侵袭相关蛋白表达。结果在胃癌组织及细胞中,circMAN1A2高表达,miR-212-3p低表达;下调circMAN1A2可以靶向上调miR-212-3p表达,且下调DDIT4表达,进而抑制胃癌细胞增殖、侵袭和迁移能力。抑制miR-212-3p表达可部分逆转抑制circMAN1A2表达对胃癌细胞恶性增殖的抑制作用。结论在胃癌细胞中抑制circMAN1A2表达可抑制胃癌细胞增殖、迁移与侵袭,其与调节miR-212-3p/DDIT4信号轴有关。 展开更多
关键词 环状RNA man1A2 微小RNA-212-3p DNA损伤诱导转录子4 胃癌 增殖 侵袭 迁移
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Development of Qinba Mountainous Area in the Historical Period from the Perspective of Man-Land Relationship
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作者 Siyu YOU Zhimin FAN 《Asian Agricultural Research》 2018年第5期85-88,共4页
Taking agricultural development as the starting point,this paper explored the dynamic mechanism and implementation path of agricultural development in Qinba Mountainous Area from the perspective of man-land relationsh... Taking agricultural development as the starting point,this paper explored the dynamic mechanism and implementation path of agricultural development in Qinba Mountainous Area from the perspective of man-land relationship. It found the regular understanding of ecological changes in Qinba Mountains. It is a comprehensive review and exploration on agricultural development and environmental evolution,and aims to provide a historical reference for the development of the contemporary Qinba Mountains. 展开更多
关键词 man-Land relationship Qinba Mountainous Area Agricultural development
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人与自然和谐共生的中国式现代化--一项基于多维度的历时性考察 被引量:1
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作者 马元喜 方盛举 《学术探索》 2024年第3期1-9,共9页
自中华人民共和国成立以来,中国的现代化建设历经传统、统筹与创新三个阶段,构成了富有中国特色的现代化建设图景。其中,关于自然价值观的更迭,从生产质料转变为保障条件,再到新型财富,其价值得以不断扩容与彰显。同时,随着自然价值观... 自中华人民共和国成立以来,中国的现代化建设历经传统、统筹与创新三个阶段,构成了富有中国特色的现代化建设图景。其中,关于自然价值观的更迭,从生产质料转变为保障条件,再到新型财富,其价值得以不断扩容与彰显。同时,随着自然价值观的变化,人与自然关系也从传统阶段的相对分离,转变为统筹阶段的共在共存,再向创新阶段的共生共荣关系抵近。在这一关系变迁中,从发展优先为起点,到发展与保护统筹兼顾,最终演变为生态优先,实现了经济效益、生态效益与社会效益的统一。纵观整个中国的现代化建设历程,绿色型发展作为通往人与自然和谐共生现代化的路径保障,实现了对传统发展模式的创造性解构,进而为通往人类现代化新形态贡献中国智慧与方案。 展开更多
关键词 中国式现代化 自然价值观 人与自然 发展与保护 绿色型发展
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MAN Development and Technical Solutions
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作者 Wei Leping (China Telecom Corporation,Beijing 100032,China) 《ZTE Communications》 2004年第1期6-10,共5页
This paper describes the major problems that Metropolitan Area Network(MAN)is facing and presents basic requirements of the next generation MAN.It presents four technical solutions:SDH,Ethernet, Resilient Packet Ring ... This paper describes the major problems that Metropolitan Area Network(MAN)is facing and presents basic requirements of the next generation MAN.It presents four technical solutions:SDH,Ethernet, Resilient Packet Ring and WDM Multiservice Platform.Based on the analysis of these solutions,it is concluded that the above technologies should be used together and different technologies should be employed under different circumstances to make an integrated solution for MAN,while SDH multiservice will play a center role in the near future. 展开更多
关键词 WDM RPR man development and Technical Solutions SDH mode MSTP
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Na^+/HCO_3^- cotransporter is expressed on β and α cells during rat pancreatic development
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作者 Li-Hua Cao Cheng-Cai Xia +5 位作者 Zhao-Chun Shi Ning Wang Zheng-Hua Gu Li-Zhi Yu Qi Wan Wei De 《World Journal of Gastroenterology》 SCIE CAS 2016年第43期9525-9533,共9页
AIM To determine the expression and localization of the electrogenic Na^+/HCO_3^- cotransporter(NBC1) in rat pancreas during development. METHODS The rat pancreas from postnatal and embryos removed from the uterus of ... AIM To determine the expression and localization of the electrogenic Na^+/HCO_3^- cotransporter(NBC1) in rat pancreas during development. METHODS The rat pancreas from postnatal and embryos removed from the uterus of pregnant rats that had been sacrificed by CO2 asphyxiation were used. Rat pancreas from embryonic day(E) 15.5 and E18.5 rat embryos was isolated under a stereomicroscope. Rat pancreas from postnatal(P) days 0, 7, 14, 21 and adult was directly isolated by the unaided eye. The RT-PCR analysis of the NBC1 specific region on rat pancreastissues from different developmental stages. The two antibodies which target the NBC1 common COOHterminal region and NH2-terminal region detected a clear band of about 145 k Da in the Western blot analysis. The localization of NBC1 was examined by immuno-fluorescence detection. RESULTS The results revealed the first peak of NBC1 expression at E18.5 and the second peak at P14. Meanwhile, the low NBC1 expression occurred at P7 and adult stages. Our results demonstrated, for the first time, the presence of NBC1 in the plasma membrane of β and α cells, as well as in the basolateral membrane of acinar cells of the rat pancreas at different stages of development. CONCLUSION The data strongly suggests that NBC1 is diversely expressed in the pancreas at different developmental stages, where it may exert its functions in pancreatic development especially islet cell growth through HCO_3^- transport and pH regulation. 展开更多
关键词 Pancreatic development β cells α cells Na+/HCO3- cotransporter IMMUNOLOCALIZATION
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Human Development from the Perspective of Ecology
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作者 Guiying zhao 《Research in Ecology》 2020年第2期32-36,共5页
the pursuit of the liberation of all mankind and the comprehensive development of human freedom is the theme of all marxist theories.Marx in the criticism of classical political economics“abstract”,to the whole hist... the pursuit of the liberation of all mankind and the comprehensive development of human freedom is the theme of all marxist theories.Marx in the criticism of classical political economics“abstract”,to the whole history of the definition of“general”,found the abstract“general”of the people behind the objective existence and state of development,so as to clearly indicate the relationship between man and nature of human development has experienced on the basis of the nature of the dependence on one stage,gradually from natural objects depends on the stage and with the natural fusion of freedom comprehensive stage,and the concrete connotation of man’s free overall and implementation path. 展开更多
关键词 man and nature development stage Needs Free and comprehensive development
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Development Strategies of E-commerce for Small and Medium - sized Enterprises
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作者 Maarina 《International Journal of Technology Management》 2016年第9期21-22,共2页
In the economic structure of our country, small and medium-sized enterprises (SMEs) are the most important part of the backbone, with the continuous popularization of the Internet, e-commerce has gradually become a ... In the economic structure of our country, small and medium-sized enterprises (SMEs) are the most important part of the backbone, with the continuous popularization of the Internet, e-commerce has gradually become a business model that the current small and medium enterprises must face and accept, The development of enterprises is of great signifi cance. In this context, the article first introduced the importance of the development of e-commerce SMEs, and then based on the actual operation of the community put forward and analyzed the current e-commerce development under the existing problems, and finally based on the actual lack of effective Development Strategy of E - commerce for Small and Medium - sized Enterprises. 展开更多
关键词 small and medium enterprises e - commerce development strategy
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National Programme for Educational Development,1996-2010
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《China Population Today》 1998年第Z2期5-6,共2页
关键词 National Programme for Educational development 1996-2010
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Special Plan for Educational Development During 2001-2005
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《China Population Today》 2002年第1期5-9,共5页
关键词 Special Plan for Educational development During 2001-2005
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China's WTO Accession and Sustainable Development ----- 2002 Report by Task Force on WTO and Environment, November 23-25, 2002, Beijing, China
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《China Population Today》 2002年第Z1期27-36,共10页
关键词 WTO China’s WTO Accession and Sustainable development November 23-25 Report by Task Force on WTO and Environment BEIJING China
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Development of Solid Rocket Motor for Manned Launch Vehicle Escape System
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作者 LI Dongchun SHI Hongbin WEI Kunlong 《Aerospace China》 2022年第1期19-26,共8页
The solid rocket motors for the escape system of China’s LM-2 F manned launch vehicle are described,the key technologies and technical innovations utilized are summarized.The technical features and development of for... The solid rocket motors for the escape system of China’s LM-2 F manned launch vehicle are described,the key technologies and technical innovations utilized are summarized.The technical features and development of foreign manned launch abort systems are also presented.The development trends of the solid rocket motor for future Chinese manned launch vehicle escape systems are proposed,which can provide a reference for the future development of manned launch vehicle escape systems. 展开更多
关键词 solid rocket motor manned launch vehicle escape system key technologies development trends
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The Programme for the Development of Chinese Women 1995-2000 Eleven Goals
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《China Population Today》 1995年第Z2期13-13,共1页
TheProgrammefortheDevelopmentofChineseWomen1995-2000ElevenGoals*Expandopportunitiesforwomen'sparticipationin... TheProgrammefortheDevelopmentofChineseWomen1995-2000ElevenGoals*Expandopportunitiesforwomen'sparticipationindecisionmakingand... 展开更多
关键词 The Programme for the development of Chinese Women 1995-2000 Eleven Goals
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Plans for the Development and Revision of Industry Standards on Inspection and Quarantine from 2005-2007
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《China Standardization》 2005年第6期8-11,共4页
The inspection and quarantine standard is one of important basics to executeinspection and quarantine by laws. In recent years, with the development of economy and trade inChina, standardization has played an importan... The inspection and quarantine standard is one of important basics to executeinspection and quarantine by laws. In recent years, with the development of economy and trade inChina, standardization has played an important role in inspection and quarantine. During 2002-2004,department of Standards of Inspection and Quarantine, Certification and Accreditation Administrationof China has organized the research of the standards of inspection and quarantine, and has builtstandard sub- systems of nine fields in inspection and quarantine. 展开更多
关键词 In Plans for the development and Revision of Industry Standards on Inspection and Quarantine from 2005-2007
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Review on Development of Chinese Power Industry in 2002-2006
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作者 Department of Research, State Electricity Regulatory Commission Jia Yulu 《Electricity》 2008年第1期13-32,共20页
The 16th National Congress of the Communist Party of China was held inNovember 2002. In the five years since then, great changes took place in Chinesepower industry. Power institutional reform was deepened; the constr... The 16th National Congress of the Communist Party of China was held inNovember 2002. In the five years since then, great changes took place in Chinesepower industry. Power institutional reform was deepened; the construction ofboth power sources and power grids was speeded up; the tense situation of powersupply and demand was alleviated; the safety and reliability of power productionwere improved; the management on power tariff was standardized; environmentalprotection and energy conservation achieved good results; researches on newtechnologies made new achievements; and the operation of enterprises was im-proved. 展开更多
关键词 Review on development of Chinese Power Industry in 2002-2006
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THE DEVELOPMENT PLANNING OF CHINA NATIONAL SERVICE STANDARD OF 2005-2008
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作者 By Wang Ruomei 《China Standardization》 2006年第2期11-12,共2页
According to the prime task of ''readjusting industrial structure,energetically developing service industry, completing service standards and promoting servicelevel'', which are raised in the 10th Five... According to the prime task of ''readjusting industrial structure,energetically developing service industry, completing service standards and promoting servicelevel'', which are raised in the 10th Five-Year Plan Outline of National Economy and SocialDevelopment, Standardization Administration of China (SAC), National Development and ReformCommission, Ministry of Civil Affairs of the People'' s Republic of China, Ministry of Constructionof P.R.C, Ministry of Commerce of the People''s Republic of China, Ministry of Culture of the People''s Republic of China, the People''s Bank of China, General Administration of Quality Supervision,Inspection and Quarantine of P.R.C (AQSIQ), the State Administration of Radio, Film and Television,State General Administration of Sport, National Tourism Administration, China Seismological Bureau,China Meteorological Administration, China Insurance Regulatory Commission, State Post Bureau, StateCultural Relics, State Administration of Traditional Chinese Medicine Bureau, China NationalTechnical Committee 264 on Service of Standardization Administration of China, China Consumers''Association and China National Institute of Standardization had compiled and officially ratified theDevelopment Planning of National Service Standard 2005-2008 by the end of 2005. 展开更多
关键词 THE development PLANNING OF CHINA NATIONAL SERVICE STANDARD OF 2005-2008
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基于FTA原理的MAN B&W 50-90MC主机电气操纵系统故障停车研究
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作者 刘建安 《中国修船》 2015年第4期50-53,56,共5页
文章对于船舶MAN B&W 50-90MC主机电气操纵系统故障停车问题,利用故障树分析(FTA)原理进行定性和定量的可靠性分析,得出关于故障停车的底事件的重要度排序,明确此系统的可靠性薄弱环节,提出预防性建议,帮助船舶轮机管理和航运公司... 文章对于船舶MAN B&W 50-90MC主机电气操纵系统故障停车问题,利用故障树分析(FTA)原理进行定性和定量的可靠性分析,得出关于故障停车的底事件的重要度排序,明确此系统的可靠性薄弱环节,提出预防性建议,帮助船舶轮机管理和航运公司机务管理人员能迅速、可以准确利用系统的故障现象定位故障源并提出解决方案,从而使系统故障可以迅速排除并恢复系统正常功能。 展开更多
关键词 故障树分析原理 故障停车 man B&W 50-90MC主机电气控制系统 顶事件 底事件 结构重要度 最小割集
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Research and Development Trend of Shape Control for Cold Rolling Strip 被引量:22
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作者 Dong-Cheng Wang Hong-Min Liu Jun Liu 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2017年第5期1248-1261,共14页
Shape is an important quality index of cold rolling strip. Up to now, many problems in the shape control domain have not been solved satisfactorily, and a review on the research progress in the shape control domain ca... Shape is an important quality index of cold rolling strip. Up to now, many problems in the shape control domain have not been solved satisfactorily, and a review on the research progress in the shape control domain can help to seek new breakthrough directions. In the past 10 years, researches and applications of shape control models, shape control means, shape detection technology, and shape con- trol system have achieved significant progress. In the aspect of shape control models, the researches in the past improve the accuracy, speed and robustness of the models. The intelligentization of shape control models should be strengthened in the future. In the aspect of the shape control means, the researches in the past focus on the roll opti- mization, mill type selection, process optimization, local strip shape control, edge drop control, and so on. In the future, more attention should be paid to the coordination control of both strip shape and other quality indexes, and the refinement of control objective should be strengthened. In the aspects of shape detection technology and shape control system, some new types of shape detection meters and shape control systems are developed and have successfully indus- trial applications. In the future, the standardization of shape detection technology and shape control system should be promoted to solve the problem of compatibility. In general,the four expected development trends of shape control for cold roiling strip in the future are intelligentization, coordi- nation, refinement, and standardization. The proposed research provides new breakthrough directions for improv- ing shape quality. 展开更多
关键词 Cold rolling strip - Shape control development trend Theory models Shape detectionControl system
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Development characteristics and orientation of tight oil and gas in China 被引量:11
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作者 SUN Longde ZOU Caineng +4 位作者 JIA Ailin WEI Yunsheng ZHU Rukai WU Songtao GUO Zhi 《Petroleum Exploration and Development》 2019年第6期1073-1087,共15页
Through reviewing the development history of tight oil and gas in China,summarizing theoretical understandings in exploration and development,and comparing the geological conditions and development technologies object... Through reviewing the development history of tight oil and gas in China,summarizing theoretical understandings in exploration and development,and comparing the geological conditions and development technologies objectively in China and the United States,we clarified the progress and stage of tight oil and gas exploration and development in China,and envisaged the future development orientation of theory and technology,process methods and development policy.In nearly a decade,relying on the exploration and development practice,science and technology research and management innovation,huge breakthroughs have been made.The laws of formation,distribution and accumulation of tight oil and gas have been researched,the development theories such as"multi-stage pressure drop"and"man-made reservoirs"have been established,and several technology series have been innovated and integrated.These technology series include enrichment regions selection,well pattern deployment,single well production and recovery factor enhancement,and low cost development.As a result,both of reserves and production of tight oil and gas increase rapidly.However,limited by the sedimentary environment and tectonic background,compared with North America,China’s tight oil and gas reservoirs are worse in continuity,more difficult to develop and poorer in economic efficiency.Moreover,there are still some gaps in reservoir identification accuracy and stimulating technology between China and North America.In the future,Chinese oil and gas companies should further improve the resource evaluation method,tackle key technologies such as high-precision 3D seismic interpretation,man-made reservoir,and intelligent engineering,innovate theories and technologies to enhance single well production and recovery rate,and actively endeavor to get the finance and tax subsidy on tight oil and gas. 展开更多
关键词 TIGHT OIL and GAS development history theoretical and TECHNOLOGICAL advancement man-made OIL and GAS reservoir enhancing recovery factor development ORIENTATION
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Bumetanide promotes neural precursor cell regeneration and dendritic development in the hippocampal dentate gyrus in the chronic stage of cerebral ischemia 被引量:1
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作者 Wang-shu Xu Xuan Sun +4 位作者 Cheng-guang Song Xiao-peng Mu Wen-ping Ma Xing-hu Zhang Chuan-sheng Zhao 《Neural Regeneration Research》 SCIE CAS CSCD 2016年第5期745-751,共7页
Bumetanide has been shown to lessen cerebral edema and reduce the infarct area in the acute stage of cerebral ischemia. Few studies focus on the effects of bumetanide on neuroprotection and neurogenesis in the chronic... Bumetanide has been shown to lessen cerebral edema and reduce the infarct area in the acute stage of cerebral ischemia. Few studies focus on the effects of bumetanide on neuroprotection and neurogenesis in the chronic stage of cerebral ischemia. We established a rat model of cerebral ischemia by injecting endothelin-1 in the left cortical motor area and left corpus striatum. Seven days later, bumetanide 200 μg/kg/day was injected into the lateral ventricle for 21 consecutive days with a mini-osmotic pump. Results demonstrated that the number of neuroblasts cells and the total length of dendrites increased, escape latency reduced, and the number of platform crossings increased in the rat hippocampal dentate gyrus in the chronic stage of cerebral ischemia. These findings suggest that bumetanide promoted neural precursor cell regeneration, dendritic development and the recovery of cognitive function, and protected brain tissue in the chronic stage of ischemia. 展开更多
关键词 nerve regeneration cerebral ischemia BUMETANIDE Na+-K+-2Cl- cotransporter 1 hippocampal dentate gyrus neurogenesis neuralprecursor cells dendritic development cognitive function neural regeneration
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Progress in China's Unconventional Oil & Gas Exploration and Development and Theoretical Technologies 被引量:34
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作者 ZOU Caineng YANG Zhi +10 位作者 ZHU Rukai ZHANG Guosheng HOU Lianhua WU Songtao TAO Shizhen YUAN Xuanjun DONG Dazhong WANG Yuman WANG Lan HUANG Jinliang WANG Shufang 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2015年第3期938-971,共34页
The new century has witnessed a strategic breakthrough in unconventional oil & gas.Hydrocarbon accumulated in micro-/nano-scale pore throat shale systems has become an important domain that could replace current oil ... The new century has witnessed a strategic breakthrough in unconventional oil & gas.Hydrocarbon accumulated in micro-/nano-scale pore throat shale systems has become an important domain that could replace current oil & gas resources.Unconventional oil & gas plays an increasingly important role in our energy demand.Tight gas,CBM,heavy oil and asphaltic sand have served as a key domain of exploration & development,with tight oil becoming a 'bright spot' domain and shale gas becoming a 'hotspot' domain.China has made great breakthroughs in unconventional oil & gas resources,such as tight gas,shale gas,tight oil and CBM,and great progress in oil shale,gas hydrate,heavy oil and oil sand.China has an estimated(223-263)×10~8t of unconventional oil resources and(890-1260)×l0^(12)m^3 of gas resources.China has made a breakthrough for progress in unconventional oil & gas study.New progress achieved in fine-grained sedimentary studies related to continental open lacustrine basin large-scale shallow-water delta sand bodies,lacustrine basin central sandy clastic flow sediments and marine-continental fine-grained sediments provide a theoretical basis for the formation and distribution of basin central reservoir bodies.Great breakthroughs have been made in unconventional reservoir geology in respect of research methodology & technology,multi-scale data merging and physical simulation of formation conditions.Overall characterization of unconventional reservoirs via multi-method and multi-scale becomes increasingly popular and facilitates the rapid development of unconventional oil & gas geological theory,method and technology.The formation of innovative,continuous hydrocarbon accumulation theory,the establishment of the framework of the unconventional oil & gas geological theory system,and the determination of the implications,geological feature,formation mechanism,distribution rule and core technology of unconventional oil& gas geological study lays a theoretical foundation for extensive unconventional oil & gas exploration and development.Theories and technologies of unconventional oil & gas exploration and development developed rapidly,including some key evaluation techniques such as 'sweet spot zone' integrated evaluation and a six-property evaluation technique that uses hydrocarbon source,lithology,physical property,brittleness,hydrocarbon potential and stress anisotropy,and some key development &engineering technologies including micro-seismic monitoring,horizontal drilling & completion and "factory-like" operation pattern, "man-made reservoir" development,which have facilitated the innovative development of unconventional oil & gas.These breakthroughs define a new understanding in four aspects:①theoretical innovation;② key technologies;③ complete market mechanism and national policy support;and ④ well-developed ground infrastructure,which are significant for prolonging the life cycle of petroleum industry,accelerating the upgrade and development of theories and technologies and altering the global traditional energy structure. 展开更多
关键词 Unconventional oil and gas Unconventional Petroleum geology fine-grainedsedimentology unconventional reservoir geology man-made reservoir" development micro-/nano- scalepore throat tight gas tight oil shale gas shale oil
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