BACKGROUND Allyl isothiocyanate(AITC),a classic anti-inflammatory and antitumorigenic agent,was recently identified as a potential treatment for obesity and insulin resistance.However,little is known about its direct ...BACKGROUND Allyl isothiocyanate(AITC),a classic anti-inflammatory and antitumorigenic agent,was recently identified as a potential treatment for obesity and insulin resistance.However,little is known about its direct impact on the liver.AIM To investigate the effect and underlying mechanism of AITC in nonalcoholic fatty liver disease(commonly referred to as NAFLD).METHODS To establish a mouse and cellular model of NAFLD,C57BL/6 mice were fed a high fat diet(HFD)for 8 wk,and AML-12 cells were treated with 200μM palmitate acid for 24 h.For AITC treatment,mice were administered AITC(100 mg/kg/d)orally and AML-12 cells were treated with AITC(20μmol/L).RESULTS AITC significantly ameliorated HFD-induced weight gain,hepatic lipid accumulation and inflammation in vivo.Furthermore,serum alanine aminotransferase and aspartate aminotransferase levels were markedly reduced in AITC-treated mice.Mechanistically,AITC significantly downregulated the protein levels of sterol regulatory elementbinding protein 1(SREBP1)and its lipogenesis target genes and upregulated the levels of proteins involved in fatty acidβ-oxidation,as well as the upstream mediators Sirtuin 1(Sirt1)and AMPactivated protein kinaseα(AMPKα),in the livers of HFD-fed mice.AITC also attenuated the nuclear factor kappa B(NF-κB)signaling pathway.Consistently,AITC relieved palmitate acid-induced lipid accumulation and inflammation in AML-12 cells in vitro through the Sirt1/AMPK and NF-κB signaling pathways.Importantly,further studies showed that the curative effect of AITC on lipid accumulation was abolished by siRNA-mediated knockdown of either Sirt1 or AMPKαin AML-12 cells.CONCLUSION AITC significantly ameliorates hepatic steatosis and inflammation by activating the Sirt1/AMPK pathway and inhibiting the NF-κB pathway.Therefore,AITC is a potential therapeutic agent for NAFLD.展开更多
The aim of this study was to evaluate the effect of allyl isothiocyanate(AITC)on the reactive oxygen species(ROS)metabolism and quality of postharvest Coprinus comatus(C.comatus).Fresh mushrooms were stored at 4℃with...The aim of this study was to evaluate the effect of allyl isothiocyanate(AITC)on the reactive oxygen species(ROS)metabolism and quality of postharvest Coprinus comatus(C.comatus).Fresh mushrooms were stored at 4℃with AITC at 5,10,and 20μL/L for 18 d,respectively.Sampling was performed every 3 d,and physicochemical parameters and ROS metabolism related enzymes activities were analyzed.Compared with the control,the application of AITC at 10μL/L significantly(P<0.05)decreased xanthine oxidase activity after 9 d of storage,while it significantly(P<0.05)improved the activities of succinic dehydrogenase,glutathione reductase,peroxidase,catalase,and ascorbate peroxidase in the middle and later stages of storage.Furthermore,the Ca^(2+)-ATPase and superoxide dismutase activities in sample treated by 10μL/L were all significantly(P<0.05)higher than those in the control.Therefore,the accumulation trends of malondialdehyde and ROS were retarded and membrane integrity was maintained.However,high-concentration AITC(20μL/L)treatment accelerated the ROS generation and increased electrolyte leakage rate.All AITC treatments significantly(P<0.05)inhibited the respiration rate during the first 9 d of storage and retarded browning of C.comatus during the storage of 18 d.These findings suggested that AITC treatment would be a promising method to maintain C.comatus quality,but the concentrations need to be optimized.展开更多
基金Supported by Natural Science Foundation of China,No.81700504 and No.81700511Science Foundation of Health Bureau of Zhejiang Province,No.2017183691+1 种基金Natural Science Foundation of Zhejiang Province,No.LY17H030006 and No.LQ15H030002Zhejiang Medical Science and Technology Project,No.2017193668
文摘BACKGROUND Allyl isothiocyanate(AITC),a classic anti-inflammatory and antitumorigenic agent,was recently identified as a potential treatment for obesity and insulin resistance.However,little is known about its direct impact on the liver.AIM To investigate the effect and underlying mechanism of AITC in nonalcoholic fatty liver disease(commonly referred to as NAFLD).METHODS To establish a mouse and cellular model of NAFLD,C57BL/6 mice were fed a high fat diet(HFD)for 8 wk,and AML-12 cells were treated with 200μM palmitate acid for 24 h.For AITC treatment,mice were administered AITC(100 mg/kg/d)orally and AML-12 cells were treated with AITC(20μmol/L).RESULTS AITC significantly ameliorated HFD-induced weight gain,hepatic lipid accumulation and inflammation in vivo.Furthermore,serum alanine aminotransferase and aspartate aminotransferase levels were markedly reduced in AITC-treated mice.Mechanistically,AITC significantly downregulated the protein levels of sterol regulatory elementbinding protein 1(SREBP1)and its lipogenesis target genes and upregulated the levels of proteins involved in fatty acidβ-oxidation,as well as the upstream mediators Sirtuin 1(Sirt1)and AMPactivated protein kinaseα(AMPKα),in the livers of HFD-fed mice.AITC also attenuated the nuclear factor kappa B(NF-κB)signaling pathway.Consistently,AITC relieved palmitate acid-induced lipid accumulation and inflammation in AML-12 cells in vitro through the Sirt1/AMPK and NF-κB signaling pathways.Importantly,further studies showed that the curative effect of AITC on lipid accumulation was abolished by siRNA-mediated knockdown of either Sirt1 or AMPKαin AML-12 cells.CONCLUSION AITC significantly ameliorates hepatic steatosis and inflammation by activating the Sirt1/AMPK pathway and inhibiting the NF-κB pathway.Therefore,AITC is a potential therapeutic agent for NAFLD.
基金supported by the National Natural Science Foundation of China(No.31871849)the Leading Talent Project of Science and Technology Innovation in Zhejiang Province(No.2019R52041),China.
文摘The aim of this study was to evaluate the effect of allyl isothiocyanate(AITC)on the reactive oxygen species(ROS)metabolism and quality of postharvest Coprinus comatus(C.comatus).Fresh mushrooms were stored at 4℃with AITC at 5,10,and 20μL/L for 18 d,respectively.Sampling was performed every 3 d,and physicochemical parameters and ROS metabolism related enzymes activities were analyzed.Compared with the control,the application of AITC at 10μL/L significantly(P<0.05)decreased xanthine oxidase activity after 9 d of storage,while it significantly(P<0.05)improved the activities of succinic dehydrogenase,glutathione reductase,peroxidase,catalase,and ascorbate peroxidase in the middle and later stages of storage.Furthermore,the Ca^(2+)-ATPase and superoxide dismutase activities in sample treated by 10μL/L were all significantly(P<0.05)higher than those in the control.Therefore,the accumulation trends of malondialdehyde and ROS were retarded and membrane integrity was maintained.However,high-concentration AITC(20μL/L)treatment accelerated the ROS generation and increased electrolyte leakage rate.All AITC treatments significantly(P<0.05)inhibited the respiration rate during the first 9 d of storage and retarded browning of C.comatus during the storage of 18 d.These findings suggested that AITC treatment would be a promising method to maintain C.comatus quality,but the concentrations need to be optimized.
