Relationship Between Leaf Structure and Aloin Content in Six Species of Aloe L.

The leaf structure, content and the storage location of aloin in the leaves of six species of Aloe L. were studied by means of semi-thin section, high performance liquid chromatography (HPLC) and fluorescent microscope. Results showed that all leaves consisted of epidermis, chlorenchyma, aquiferous tissue and vascular bundles. The leaves had the xeromorphic characteristics, including thickened epidermal cell wall, thickened cuticle, sunken stomata and well-developed aquiferous tissue. With the exception of thus, there were remarkable differences in leaf structure among the six species. The chlorenchyma cells were similar to palisade tissues in Aloe arborescens Mill. and A. mutabilis Pillans, but isodiametric in A. vera L., A. vera L. var. chinensis Berg., A. saponaria Hawer and A. greenii Bali. A. arborescens, A. mutabilis, A. very and A. vera var. chinensis included large parenchymatous cells at the vascular bundles, whereas no such cells were observed at the vascular bundles of A. saponaria and A. greenii. In A. arborescens, A. mutabilis and A. vera, the aquiferous tissue sheaths were present and composed of a layer of small parenchymatous cells without chloroplasts around the aquiferous tissue. While there were no aquiferous tissue sheaths in A. vera var. chinensis, A. saponaria and A. greenii. The HPLC revealed that the content of aloin was high in A. arborescens, low in A. vera, and very low in A. saponaria among the six species. The fluorescent microscopy showed that the yellow-green globule only appeared in the large parenchymatous cells of vascular bundles, vascular bundle sheath and aquiferous tissue sheath, but not in the chlorenchyma and aquiferous tissue. Consequently, the large parenchymatous cells of vascular bundles, vascular bundle sheath and aquiferous tissue sheath were the storage location of aloin. They were positively correlated with the content of aloin.

木立芦荟不同叶龄叶的解剖结构和芦荟素含量的测定

目的 旨在揭示芦荟素在不同叶龄芦荟叶中的含量及其差异的原因。方法 用高效液相色谱法测定芦荟素的含量。用半薄切片法研究叶的解剖结构。结果 在同一株植物内 ,从上到下随着叶龄的增大 ,叶片维管束中的大型薄壁细胞逐渐萎缩 ,芦荟素的含量逐渐降低。

不同品种芦荟中芦荟苷的含量测定

目的建立测定芦荟中芦荟苷含量的高效液相色谱(HPLC)法,测定不同芦荟品种中芦荟苷的含量。方法用超声振荡提取法制备样品溶液,并测定芦荟苷的含量。结果芦荟苷线性回归方程为A=13.168 4 m-2.855 4,r=0.999 8,进样量在0.06～0.93μg范围内与峰面积线性关系良好,平均回收率为101.42%,RSD为2.39%。测定的各种芦荟中芦荟苷含量分别为,华芦荟72.92μg/g,不夜城芦荟2.622μg/g,木立芦荟614.3μg/g,库拉索芦荟135.3μg/g,元江芦荟212.5μg/g。结论不同品种芦荟中芦荟苷的含量不同,木立芦荟中芦荟苷含量最高。所用检测方法简便、准确快速,可作为芦荟中芦荟苷含量的测定方法。

芦荟质量的实验研究

目的:初步探讨芦荟的质量差异。方法:按照中国药典方法对样品进行理化鉴别及含量测定;用建立的HPLC法测定了样品中芦荟苷的含量。结果:6份芦荟中有2份不符合药典规定;芦荟药材平均回收率为99.49%,RSD为1.26%(n=5)。结论:药材的外观与内在质量有一定的相关性;HPLC法测定芦荟苷单一成分,方法简便、准确、灵敏,专属性强,可用于芦荟和制剂中芦荟苷的含量检测。

槲-芦美白防晒凝胶的制备及体外透皮行为考察

目的:以槲皮素和芦荟苷为原料,制备复方美白防晒凝胶并考察其体外透皮吸收行为。方法:以卡波姆940为基质,月桂氮艹卓酮为促渗剂,以丙二醇和甘油为保湿剂,采用单因素试验优选制剂的最佳处方并制备槲-芦美白防晒凝胶,考察复方凝胶的外观、黏稠度、流变性、pH及稳定性。建立芦荟苷、槲皮素的HPLC含量测定方法,采用Franze扩散池法,考察复方凝胶中芦荟苷和槲皮素的体外经皮渗透特性。结果:制备的槲-芦美白防晒凝胶外观透明,黏稠度、流变性适中,稳定性较好。芦荟苷、槲皮素检测浓度分别在8.36~83.60"g·ml^-1(r=0.9999)、7.565~75.650μg·ml^-1(r=0.9999)范围内线性关系良好;平均加样回收率分别为99.54%和100.44%,RSD分别为0.34%和0.93%(n=9)。两者24 h累积渗透量分别为660.139μg·cm^-2和520.835μg·cm^-2,其24 h累积透过率分别为(88.05±1.63)%和(70.70±1.52)%。结论:用于测定复方凝胶中芦荟苷、槲皮素的含量测定方法操作简单,快速准确;两主药成分透皮吸收特性良好,为后期的药效实验提供依据。

芦荟配方颗粒质量标准研究

目的：建立芦荟配方颗粒质量标准。方法：采用薄层色谱法（TLC）对芦荟配方颗粒中芦荟苷进行鉴别，采用醇溶性浸出物测定法中的热浸法测定浸出物；采用高效液相色谱法（HPLC）对芦荟配方颗粒中的芦荟苷进行测定，并对芦荟配方颗粒各项指标进行检查。结果：TLC法中芦荟配方颗粒在与芦荟苷对照品相应位置上呈现相同颜色的斑点；浸出物限度拟定为不少于17．0％；含量测定中，芦荟苷的线性范围为0．1612～1．6120μg（r=0．9998），平均回收率为98．48％，RSD=0．53％，（n=6），并拟定芦荟苷的质量分数应不少于16．0mg·g^-1。芦荟配方颗粒水分、溶化性、粒度均达到《中华人民共和国药典》颗粒剂的要求。结论：该方法可行，重复性好，能有效控制芦荟配方颗粒的质量。

HPLC法测定芦荟胶囊中芦荟苷的含量

目的:建立芦荟胶囊中芦荟苷的含量测定方法。方法:采用HPLC法,色谱柱为Promosil C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-水(24∶76);流速为0.8 mL.min-1;检测波长为355 nm;柱温为室温,进样量为10μL。结果:芦荟苷在0.01673-0.8365μg范围内呈良好的线形关系(r=0.9999),平均回收率为96.5%,RSD%为1.0%(n=6)。结论:该方法操作简单、准确,重现性好,可用于芦荟胶囊的质量控制。

高效液相色谱法测定芦荟配方颗粒中芦荟苷的含量

目的建立芦荟配方颗粒芦荟苷的高效液相色谱分析方法。方法 Shim-pack CLC-ODS-C18柱（6.0×150mm,10μm）;流动相：乙腈-水（25∶75）,流速：1.0m L·min^-1;柱温：35℃;检测波长355nm。结果芦荟苷在0.1612~1.6120μg范围内有良好的线性关系。平均回收率为98.50%（RSD=0.35%,n=6）。结论该方法简便快捷、准确、无干扰,可作为芦荟配方颗粒的内控质量标准。