Clinicopathological alterations in wild mammals from the reservoir system of Trypanosoma cruzi:a scoping review

Trypanosoma cruzi is the etiologic agent of Chagas disease.This flagellated protozoan is transmitted to humans as well as different species of domestic and wild animals via vectors from the Reduviidae family(known as"kissing bugs").Despite the fact that hundreds of species of wild mammals are part of the reservoir system,the morphologi-cal changes and clinical manifestations resulting from the pathogenesis of the infection have been largely neglected.The aim of this review is to systematically compile the available information regarding clinicopathological altera-tions in wild mammals due to natural infection by T.cruzi.Information was obtained from six online bibliographic data search platforms,resulting in the identification of 29 publications that met the inclusion criteria.Mortality was the most common clinical manifestation,cardiac damage was the main finding at necropsy,and lymphoplas-macytic inflammation was the most frequent microscopic injury.Thus,regardless of its role as a reservoir,T.cruzi has the potential to affect the health status of wild mammals,a situation that highlights the need for further research to analyze,measure,and compare its effects at both the individual and population levels.

Risk stratification for radioactive iodine refractoriness using molecular alterations in distant metastatic differentiated thyroid cancer

Objective: Patients with radioactive iodine-refractory differentiated thyroid cancer(RAIR-DTC) are often diagnosed with delay and constrained to limited treatment options. The correlation between RAI refractoriness and the underlying genetic characteristics has not been extensively studied.Methods: Adult patients with distant metastatic DTC were enrolled and assigned to undergo next-generation sequencing of a customized 26-gene panel(Thyro Lead). Patients were classified into RAIR-DTC or non-RAIR groups to determine the differences in clinicopathological and molecular characteristics. Molecular risk stratification(MRS) was constructed based on the association between molecular alterations identified and RAI refractoriness, and the results were classified as high, intermediate or low MRS.Results: A total of 220 patients with distant metastases were included, 63.2% of whom were identified as RAIRDTC. Genetic alterations were identified in 90% of all the patients, with BRAF(59.7% vs. 17.3%), TERT promoter(43.9% vs. 7.4%), and TP53 mutations(11.5% vs. 3.7%) being more prevalent in the RAIR-DTC group than in the non-RAIR group, except for RET fusions(15.8% vs. 39.5%), which had the opposite pattern. BRAF and TERT promoter are independent predictors of RAIR-DTC, accounting for 67.6% of patients with RAIR-DTC. MRS was strongly associated with RAI refractoriness(P<0.001), with an odds ratio(OR) of high to low MRS of 7.52 [95%confidence interval(95% CI), 3.96-14.28;P<0.001] and an OR of intermediate to low MRS of 3.20(95% CI,1.01-10.14;P=0.041).Conclusions: Molecular alterations were associated with RAI refractoriness, with BRAF and TERT promoter mutations being the predominant contributors, followed by TP53 and DICER1 mutations. MRS might serve as a valuable tool for both prognosticating clinical outcomes and directing precision-based therapeutic interventions.

Hydrothermal alteration of the surface volcanic rocks at the Acoculco geothermal field,Mexico:a multi-parametric approach

The studies on hydrothermal alteration-induced eff ects in surface and subsurface rocks provide useful information in the characterization and exploitation of a geothermal reservoir.Generally,these studies are based on traditional,and reliable methods like petrography(primary and secondary minerals,and grade of alteration),and geochemistry(mobility of elements,changes in mass and concentration of elements,and fluid inclusions).Recently,apart from these established methods,some methods based on the geochemical(Chemical Index of Alteration,CIA;Weathering Index of Parkar,WIP;Loss on Ignition,LOI;and Sulfur,S)and rock magnetic properties(magnetic susceptibility,χlf;and percentage frequency-dependent susceptibility,χfd%)are also being applied in the identification of whether a rock is an altered or a fresh one.The Acoculco Geothermal Field(AGF),Mexico,is characterized by high temperature and very low permeability,and it is considered a promissory Enhanced Geothermal System.The following changes are observed in the rocks as a result of an increase in hydrothermal alteration:(1)an increase in CIA,LOI,and S values,and a decrease in WIP;(2)an increase in quartz and quartz polymorph minerals(silicification),and clay minerals(argillization);and(3)decrease inχlf values.At AGF,the most altered surface acid rocks are characterized by entirely quartz and its polymorphs,and clay minerals.The present study also indicates the applicability of the binary plots of major elements(felsic vs mafic component)and rock magnetic parameters(χlf vs.χfd%).The rock withχfd%value of 2-10 andχlf value<0.5×10^(-6)m^(3) kg^(-1)indicate the presence of single domain and stable single domain grains,which in turn suggests that it is an altered rock.These methods are simple to apply,rapid,reliable,and have the potential to become eff ective tools for the identifi cation of hydrothermally altered rocks during the initial stage of geothermal exploration.

Identifying Comprehensive Genomic Alterations and Potential Neoantigens for Cervical Cancer Immunotherapy in a Cohort of Chinese Squamous Cell Carcinoma of the Cervix

Objective Genomic alterations and potential neoantigens for cervical cancer immunotherapy were identified in a cohort of Chinese patients with cervical squamous cell carcinoma(CSCC).Methods Whole-exome sequencing was used to identify genomic alterations and potential neoantigens for CSCC immunotherapy.RNA Sequencing was performed to analyze neoantigen expression.Results Systematic bioinformatics analysis showed that C>T/G>A transitions/transversions were dominant in CSCCs.Missense mutations were the most frequent types of somatic mutation in the coding sequence regions.Mutational signature analysis detected signature 2,signature 6,and signature 7 in CSCC samples.PIK3CA,FBXW7,and BICRA were identified as potential driver genes,with BICRA as a newly reported gene.Genomic variation profiling identified 4,960 potential neoantigens,of which 114 were listed in two neoantigen-related databases.Conclusion The present findings contribute to our understanding of the genomic characteristics of CSCC and provide a foundation for the development of new biotechnology methods for individualized immunotherapy in CSCC.

Genomic alterations in oral multiple primary cancers

Oral squamous cell carcinoma (OSCC) is the predominant type of oral cancer, while some patients may develop oral multiple primary cancers (MPCs) with unclear etiology. This study aimed to investigate the clinicopathological characteristics and genomic alterations of oral MPCs. Clinicopathological data from patients with oral single primary carcinoma (SPC, n=202) and oral MPCs (n=34) were collected and compared. Copy number alteration (CNA) analysis was conducted to identify chromosomal-instability differences among oral MPCs, recurrent OSCC cases, and OSCC patients with lymph node metastasis. Whole-exome sequencing was employed to identify potential unique gene mutations in oral MPCs patients. Additionally, CNA and phylogenetic tree analyses were used to gain preliminary insights into the molecular characteristics of different primary tumors within individual patients. Our findings revealed that, in contrast to oral SPC, females predominated the oral MPCs (70.59%), while smoking and alcohol use were not frequent in MPCs.Moreover, long-term survival outcomes were poorer in oral MPCs. From a CNA perspective, no significant differences were observed between oral MPCs patients and those with recurrence and lymph node metastasis. In addition to commonly mutated genes such as CASP8, TP53 and MUC16, in oral MPCs we also detected relatively rare mutations, such as HS3ST6 and RFPL4A. Furthermore, this study also demonstrated that most MPCs patients exhibited similarities in certain genomic regions within individuals, and distinct differences of the similarity degree were observed between synchronous and metachronous oral MPCs.

Sex-dependent alterations in extracellular vesicles linking chronic spinal cord injury to brain neuroinflammation and neurodegeneration

Traumatic spinal cord injury(SCI)is a devastating exogenous injury with long-lasting consequences and a leading cause of death and disability worldwide.Advances in assistive technology,rehabilitative interventions,and the ability to identify and intervene in secondary conditions have significantly increased the long-term survival rate of SCI patients,with some people even living well into their seventh or eighth decade.These survival changes have led neurotrauma researchers to examine how SCI interacts with brain aging.Public health and epidemiological data showed that patients with long-term SCI can have a lower life expectancy and quality of life,along with a higher risk of comorbidities and complications.

Bilateral meibomian gland morphological alterations in unilateral herpes simplex keratitis based on artificial intelligence analysis

AIM:To explore whether unilateral herpes simplex keratitis(HSK)can cause morphological changes of bilateral meibomian glands(MGs)based on artificial intelligence(AI)analytical system.METHODS:In the retrospective study,29 patients with unilateral HSK and 29 participants matched in terms of age and sex were included as control group.Meibographic images of the upper eyelid using Keratograph 5M and assessed ocular surface parameters including tear meniscus height and tear break-up time.MG density and vagueness values were automatically analyzed and calculated using an AI analytical system.We compared the differences between the affected and the contralateral unaffected eyes in HSK patients,and the normal control eyes.We employed either the paired t-test or the Wilcoxon signed-rank test to compare significant difference between the affected and unaffected eyes in HSK patients or between the HSK group and control group.RESULTS:The MG density was 0.19±0.09 in the HSKaffected eye and 0.18±0.07 in contralateral unaffected eye,which had no significant difference(P=0.616).The MG density between the affected eye with HSK and the normal control group was statistically significant(P=0.028).There was a significant difference in MG density between the contralateral unaffected eye and the normal control group(P=0.012).However,no significant difference in vagueness value was observed between the eye with HSK and the control group or between HSK eye and contralateral eye.CONCLUSION:The MG density between the HSKaffected eye and the contralateral unaffected eye don’t significantly differ,whereas there is a significant decrease in the HSK group compared to that of the normal participants.Unilateral HSV keratitis may suffer from bilateral changes of MG morphology indicating bilateral dry eye.Therefore,the fellow eye of patients with unilateral HSK should be considered a potential case of MG dysfunction,necessitating early treatment for bilateral dry eye in the clinic.

Application of Remote Sensing and GIS in Mineral Alteration Mapping and Lineament Extraction Case of Oudiane Elkharoub (Requibat Shield, Northern of Mauritania)

The integration of remotely sensed data allowed the successful characterization of the mineral alteration zones of the Oudiane Elkharoub area in the Northeastern part of Reguibat Shield using image transformation techniques. As both chemical and geochemical analyses showed significant Au, Ag, Cu, Pb, Mn, Cr, Ni, Th and Y anomalies, it’s very interesting to apply the remote sensing and GIS in mineral resources mapping. The remote sensing is a direct adjunct to the field, lithologic and structural mapping, and more recently, GIS has played an important role in the study of mineralization areas. The integration of several evidential maps highlighted the plausible areas with high concentrations of chlorite, epidote, kaolinite, calcite, alunite, hematite, illite and sulfur among other key mineral alterations that reflect the intensity of hydrothermal effects and the probable sites of ore bodies. The methodological approach integrates geological information acquired from Aster and Landsat 8 OLI/TIRS (Operational Land Imager/Thermal InfraRed Sensor) images and a multi-criteria GIS analysis. The superimposition of various lineament and hydrothermal alteration maps and the consideration of precious and base metal indicators allowed the zoning of sites likely to contain mineral concentrations. Remote sensing becomes an important tool for locating mineral deposits in its own right, when the primary and secondary processes of mineralization result in the formation of spectral anomalies. Reconnaissance lithological mapping is usually the first step of mineral resource mapping. This is complimented with structural mapping, as mineral deposits usually occur along or adjacent to geologic structures, and alteration mapping, as mineral deposits are commonly associated with hydrothermal alteration of the surrounding rocks. Ground truthing and laboratory studies including XRD analysis were utilized to verify the results.

Hematological Alterations in an Eastern Sudanese Chronic Kidney Disease Patient Population

Background: Chronic Kidney Disease (CKD), associated with a slow and progressive loss of kidney function over a period of several years, is an important clinical disaster with an increasing rate of morbidity and mortality especially in the least developed countries. Many hematological parameters are thought to alter dramatically during the course of the disease. These include white blood cells, red blood cells, and platelets. Methods: We tried, retrospectively, to evaluate the peripheral blood hematological alterations in a group of patients undergoing hemodialysis in an eastern Sudan dialysis center to add local medical information. Results: Anemia (Low hemoglobin and hematocrit) was detected in 94% of the patients’ group. Mean Erythrocyte count (3.32vs.4.76 (×109/L)), Hemoglobin concentration (9.4vs.13 (g/dl)), Hematocrit (28.7vs.38.7 (L/L)) and platelet count (296 vs. 238 (×109/L)) were significantly lower in the patients’ group than in the control group (P-values Conclusion: Five out of eight studied parameters (Red cell count, hemoglobin, hematocrit, mean cell hemoglobin concentration, and platelets count) have shown a significant alteration in CKD patients. As the complete blood count (CBC) test is the most utilized test in clinical laboratory practice, these alterations may be considered as early indicators for CKD. Furthermore, all patients with CKD must be routinely checked for these alterations.

Evaluation of the coupled impact of silicon oxide nanoparticles and low-salinity water on the wettability alteration of Berea sandstones

This study investigated experimentally the coupled effects of hydrophilic SiO_(2) nanoparticles(NPs)and low-salinity water(LSW)on the wettability of synthetic clay-free Berea sandstone.Capillary pressure,interfacial tension(IFT),contact angle,Zeta potential,and dynamic displacement measurements were performed at various NP mass fractions and brine salinities.The U.S.Bureau of Mines(USBM)index was used to quantify the wettability alteration.Furthermore,the NP stability and retention and the effect of enhanced oil recovery by nanofluid were examined.The results showed that LSW immiscible displacement with NPs altered the wettability toward more water wet.With the decreasing brine salinity and increasing NP mass fraction,the IFT and contact angle decreased.The wettability alteration intensified most as the brine salinity decreased to 4000 mg/L and the NP mass fraction increased to 0.075%.Under these conditions,the resulting incremental oil recovery factor was approximately 13 percentage points.When the brine salinity was 4000 mg/L and the NP mass fraction was 0.025%,the retention of NPs caused the minimum damage to permeability.

Spectrum of fluid/rock alteration in the IOCG system of eastern Gawler Craton

In this paper we present petrology and geochemistry from a range of metasomatically altered rocks within the Eastern Gawler Craton mineralised province of South Australia.The rocks represent four protolith types;granite,basalt,non-carbonate metasediment and calc-silicate metasediment.Alteration paragenesis varies in intensity and between the protolith types.I.The alteration paragenesis of granites has the first pervasive chlorite and the second filled hematite with two generations of chalcopyrite.After the

Potential mechanisms of non-suicidal self-injury(NSSI)in major depressive disorder:a systematic review

Background Non-suicidal self-injury(NSSI)is a frequent and prominent phenomenon in major depressive disorder(MDD).Even though its prevalence and risk factors are relatively well understood,the potential mechanisms of NSSI in MDD remain elusive.Aims To review present evidence related to the potential mechanisms of NSSI in MDD.Methods According to Preferred Reporting Items for Systematic Reviews and Meta-Analyses 2020 guidelines,articles for this systematic review were searched on Medline(through PubMed),Embase(through Elsevier),PsycINFO(through OVID)and Web of Science databases for English articles,as well as China National Knowledge Infrastructure(CNKI),SinoMed,Wanfang Data,and the Chongqing VIP Chinese Science and Technology Periodical(VIP)Databases for Chinese articles published from the date of inception to 2 August 2022.Two researchers(BW,HZ)independently screened studies based on inclusion and exclusion criteria and assessed their quality.Results A total of 25157 studies were searched.Only 25 of them were ultimately included,containing 3336 subjects(1535 patients with MDD and NSSI,1403 patients with MDD without NSSI and 398 HCs).Included studies were divided into 6 categories:psychosocial factors(11 studies),neuroimaging(8 studies),stress and hypothalamic-pituitary-adrenal(HPA)axis(2 studies),pain perception(1 study),electroencephalogram(EEG)(2 studies)and epigenetics(1 study).Conclusions This systematic review indicates that patients with MDD and NSSI might have specific psychosocial factors,aberrant brain functions and neurochemical metabolisms,HPA axis dysfunctions,abnormal pain perceptions and epigenetic alterations.

Wettability alteration by magnesium ion binding in heavy oil/brine/chemical/sand systems—analysis of hydration forces

In laboratory sandpack tests for heavy oil re-covery by alkaline flooding, it was found that wettability alteration of the sand had a significant impact on oil recovery. In this work, a heavy oil of 14? API was used to examine the effect of organic acids in the oil and water che- mistry on wettability alteration. From interfacial tension measurements and sand surface composition analysis, it was concluded that the water-wet sand became preferentially oil-wet by magnesium ion binding. The presence of Mg2+ in the heavy oil/Na2CO3 solution/sand system increased the oil/water interfacial tension. This confirmed the hypothesis that magnesium ion combined with the ionized organic acids to form magnesium soap at oil/water interface. Under alkaline condition, the ionized organic acids in the oil phase partition into the water phase and subsequently adsorb on the sand surfaces. The analysis of sand surface composition sugg- ested that more ionized organic acids adsorb- ed on the sand surface through magnesium ion binding. The attachment of more organic acids on the sand surface changed hydration forces, making the sand surface more oil-wet.

Wettability alteration of organo-vermiculites induced by layer charge and tailored bis-N-heterocyclic quaternary ammonium salts

Wettability is an important surface property that deserves to further explore the factors on its alteration.Series of bis-N-heterocyclic quaternary ammonium salts with different spacer length and N-heterocyclic headgroups(morpholinium(BMMB,BMMD and BMMH),piperidinium(BPMH)and piperazinium(BMPMH))have been synthesized and employed for altering the wettability of vermiculite and its derivates(Vts)treated by Li^(+)-saturated heating method.The results of X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FT-IR),thermogravimetric analysis(TG-DTG),scanning electron microscopy(SEM)and N_(2)adsorption/desorption isotherms indicate that all of the bis-N-heterocyclic quaternary ammonium salts have been successfully inserted into the vermiculite layers,leading to the organic monolayer.The results of capillary rise tests combined with Lipophilic to Hydrophilic Ratio(LHR)values unveil the wettability alteration of the organo-Vts.As the layer charge decreases,the hydrophilicity of the organo-Vts gradually increases,which is probably caused by the decline in binding sites.As the result of the change in spacer length of modifier,the wetting properties of morpholinium-based organo-Vts change in order of BMMD-Vts>BMMH-Vts>BMMB-Vts,and difference in N-heterocyclic headgroups leads to the sequence of wettability:BMPMH-Vts>BPMH-Vts>BMMH-Vts.Layer charge of Vt,spacer length and the type of the N-heterocyclic headgroup of modifier have the synergistic effect on the regulation of the wettability.

Combined imbibition system with black nanosheet and low-salinity water for improving oil recovery in tight sandstone reservoirs

Nanomaterials and low-salinity water(LSW)are two popular enhanced oil recovery(EOR)methods that have been widely studied in recent years.The former is used for in-depth conformance improvement and the latter for microscopic oil displacement(by altering the potential and contact angle).However,there are few literature on combining them to achieve synergistic effects,especially for tight sandstone res-ervoirs.Based on the reservoir conditions of the Jimusar Oilfield,this study investigated the oil recovery mechanism of the combined imbibition system,which was composed of black nanosheet(BN)and LSW.Its performances including decreasing interfacial tension,emulsification,and wettability alterations were evaluated.The imbibition differences between the single system of BN and LSW and the combined BN-LsW imbibition system were then compared.Results showed that the combined imbibition system had a better emulsification effect on the crude oil and could also alter the wettability of the core surface.Moreover,the combined system could increase both the imbibition rate and the ultimate oil recovery.The nuclear magnetic T2 spectrum also indicated that the addition of black nanosheets could divert more fluid into small pores and thus improve the microscopic sweep efficiency.

Investigation of the effect of diethylene triamine pentaacetic acid chelating agent as an enhanced oil recovery fluid on wettability alteration of sandstone rocks

This study used the diethylene triamine pentaacetic acid(DTPA)-seawater(SW)system to modify the sandstone rock wettability and enhance oil recovery.The investigation involved conducting wettability measurement,Zeta potential measurement,and spontaneous imbibition experiment.The introduction of 5%DTPA-sW solution resulted in a significant decrease in the rock-oil contact angle from 143°to 23,along with a reduction in the Zeta potential from-2.29 mV to-13.06 mV,thereby altering the rock surface charge and shifting its wettability from an oil-wet state to a strongly water-wet state.The presence or absence of potential determining ions(Ca^(2+),Mg^(2+),SO_(4)^(2-))in the solution did not impact the effectiveness of DTPA in changing the rock wettability.However,by tripling the concentration of these ions in the solution,the performance of 5%DTPA-SW solution in changing wettability was impaired.Additionally,spontaneous imbibition tests demonstrated that the 5%DTPA-SW solution led to an increase in oil recovery up to 39.6%.Thus,the optimum mass fraction of DTPA for changing sandstone wettability was determined to be5%.

Synaptic alterations as a common phase in neurological and neurodevelopmental diseases: JNK is a key mediator in synaptic changes

Brain synapses play a key role in neuronal communication:this“conversation”is at the basis of all brain activities and synaptic dysfunction leads to brain disorders.We study the modulators of this crucial synaptic function and we here present the evidence supporting the c-Jun N-terminal kinase(JNK)pathway as a pivotal actor in this scenario.

Insight into the evolution of breast cancer driven by genetic alterations

Breast cancer originates primarily from the epithelial cells of the mammary gland.Repeated mammary gland expansion and degeneration are accompanied by an increased risk of genetic alterations in the breast1.These mutations in breast epithelial cells dynamically occur in response to pregnancy,labor and delivery,breastfeeding,and the menstrual cycle,with a decline in mutation rates after menopause,which may be related to a decrease in estrogen levels.The breast epithelial cell mutations are also consistent with epidemiologic observations2.

Asian variant intravascular large B-cell lymphoma with highly suspected central nervous system involvement:A case report

BACKGROUND Intravascular large B-cell lymphoma(IVLBCL)is a rare subtype of extranodal lymphoma.In particular,the Asian variant of IVLBCL is characterized by hemophagocytic lymphohistiocytosis along with bone marrow involvement.However,central nervous system(CNS)involvement is uncommon in this variant compared to the Western variant.Here,we report a case of typical Asian variant IVLBCL with highly suspected CNS involvement and discuss the nature of the disease and its genetic aberration.CASE SUMMARY A 67-year-old female patient complained of gradually worsening cognitive impairment.While hospitalized,she developed a high fever and showed marked bicytopenia.Intracranial imaging revealed a suspected leptomeningeal disease.Although no malignant cells were found in the cerebrospinal fluid(CSF),the protein and lactate dehydrogenase levels in CSF were increased.Bone marrow examination revealed an increased number of hemophagocytic histiocytes,and 18F-fluorodeoxyglucose(FDG)positron emission tomography with computerized tomography scan revealed increased FDG uptake in both adrenal glands,the liver,and the right ethmoid sinus.A tissue biopsy showed atypical large lymphoid cells with prominent nucleoli in the vessels,and the tumor cells were positive for CD20,BCL2,BCL6,and IRF4/MUM1.In addition,targeted sequencing identified MYD88,TET2,and PIM1 mutations.Consequently,we diagnosed the patient with the Asian variant of IVLBCL with highly suspected CNS involvement.CONCLUSION Suspicion of IVLBCL and immediate diagnosis lead to timely treatment.Moreover,careful CNS examination at diagnosis is recommended.

Integrating Aster Images Processing and Fieldwork for Identification of Hydrothermal Alteration Zones at the Oumjrane-Boukerzia District, Moroccan Anti-Atlas

Mapping from remote sensing has become more effective in the field of geology, mainly in lithological discrimination and identification of hydrothermal alteration zones. The use of this technique consists in obtaining information about the rock mass and the main ones existing in the inaccessible areas. Satellite data from the ASTER (Advanced Spaceborne Thermal Emission and Reflection Radiometer) sensor represent a favorable potential for detecting the spectral signatures of mineral zones and identifying their nature. These data are more reliable in places where the climate is arid with less abundant vegetation, as at the Oumjrane-Boukerzia mining district. This region which is part of the Eastern Anti-Atlas, is composed of several mineralized veins which still require detailed studies and exploration by the technique of remote sensing. In this work we applied several processing techniques on ASTER imagery such as Colored Composition, Principal Component Analysis and Ratio Bands. The use of the reports of the specialized Bands makes it possible to identify some hydrothermal alteration minerals within the mining district of Oumjrane Boukerzia. These minerals are represented mainly by iron oxides and hydroxides (Hematite, jarosite, limonite and goethite), carbonate minerals (dolomite, calcite), clay minerals (Illite, kaolinite and chlorite) and quartz minerals. This work allows us to produce a map of hydrothermal alteration zones which can be used as a valuable reference in the strategy of mining exploration for the base metals (Cu, Pb, Zn and Ba), in the mining district of Oumjrane-Boukerzia and in the entire Eastern Anti-Atlas.