Effect of fertigation frequency on soil nitrogen distribution and tomato yield under alternate partial root-zone drip irrigation

Alternate partial root-zone drip fertigation (ADF) is a combination of alternating irrigation and drip fertigation,with the potential to save water and increase nitrogen (N) fertilizer efficiency.A 2-year greenhouse experiment was conducted to evaluate the effect of different fertigation frequencies on the distribution of soil moisture and nutrients and tomato yield under ADF.The treatments included three ADF frequencies with intervals of 3 days (F3),6 days (F6) and 12 days (F12),and conventional drip fertigation as a control (CK),which was fertilized once every 6 days.For the ADF treatments,two drip tapes were placed 10 cm away on each side of the tomato row,and alternate drip irrigation was realized using a manual valve on the distribution tapes.For the CK treatment,a drip tape was located close to the roots of the tomato plants.The total N application rate of all treatments was 180 kg ha^(-1).The total irrigation amounts applied to the CK treatment were450.6 and 446.1 mm in 2019 and 2020,respectively;and the irrigation amounts applied to the ADF treatments were 60%of those of the CK treatment.The F3 treatment resulted in water and N being distributed mainly in the 0–40-cm soil layer with less water and N being distributed in the 40–60-cm soil layer.The F6 treatment led to 21.0 and 29.0%higher 2-year average concentration of mineral N in the 0–20 and 20–40-cm soil layer,respectively and a 23.0%lower N concentration in the 40–60-cm soil layer than in the CK treatment.The 2-year average tomato yields of the F3,F6,F12,and CK treatments were 107.5,102.6,87.2,and 98.7 t ha^(-1),respectively.The tomato yield of F3 was significantly higher (23.3%) than that in the F12 treatment,whereas there was no significant difference between the F3 and F6 treatment.The F6 treatment resulted in yield similar to the CK treatment,indicating that ADF could maintain tomato yield with a 40%saving in water use.Based on the distribution of water and N,and tomato yield,a fertigation frequency of 6 days under ADF should be considered as a water-saving strategy for greenhouse tomato production.

Influences of alternate partial root-zone irrigation and urea rate on water-and nitrogen-use efficiencies in tomato

Traditional water and fertilizer inputs are often much higher than the actual demands of tomato,which causes a reduction in water-and fertilizer-use efficiencies.To investigate the advantage of alternate partial root-zone irrigation(AI)on water-and nitrogen(N)-use efficiencies of tomato modified by water and N management,taking conventional irrigation(CI)as the control,the effects of AI on root morphology and activity,fruit yield and water and N use efficiency were studied using pot experiments.There were four combinations of irrigation levels and growing stages of tomato for AI,i.e.AI_(1)(high water(W_(H))from blooming to harvest stage(BHS)),AI_(2)(W_(H)from blooming to fruit setting stage(BFS)and low water(W_(L))at the harvest stage(HS)),AI_(3)(W_(L)at BFS and W_(H)at HS)and AI_(4)(W_(L)at BHS)at three urea rates,i.e.low urea rate(NL),middle urea rate(N_(M))and high urea rate(N_(H))in the form of urea.Irrigation quotas for W_(H)and W_(L)in AI at BFS or HS were 80%and 60%of that in CI,respectively.Compared to CI,AI decreased water consumption by 16.0%-33.1%and increased water use efficiency of yield(WUE_(y))and dry mass(WUE_(d))by 6.7%-11.9%and 10.2%-15.9%,respectively.AI_(1)did not decline yield,total N uptake(TNU)and N use efficiency(NUE)significantly.Compared to NL,N_(M)enhanced tomato yield,TNU,WUE_(y)and WUE_(d)by 28.5%,35.3%,22.6%and 16.3%,respectively.Compared to CINL,AI_(1)N_(M)reduced water consumption by 12.5%,but increased tomato yield,TNU,WUE_(y)and WUE_(d)by 35.5%,58.4%,54.4%and 53.7%,respectively.Therefore,AI_(1)can improve water use efficiency and total N uptake of tomato simultaneously at medium urea rate.

Alternate partial root-zone irrigation with high irrigation frequency improves root growth and reduces unproductive water loss by apple trees in arid north-west China

Alternate partial root-zone irrigation(APRI)can improve water use efficiency in arid areas. However,the effectiveness and outcomes of different frequencies of APRI on water uptake capacity and physiological water use have not been reported. A two-year field experiment was conducted with two irrigation amounts(400 and500 mm) and three irrigation methods(conventional irrigation, APRI with high and low frequencies). Root length density, stomatal conductance, photosynthetic rate,transpiration rate, leaf water use efficiency, midday stem and leaf water potentials were measured. The results show that in comparison with conventional irrigation, APRI with high frequency significantly increased root length density and decreased water potentials and stomatal conductance.No differences in the above indicators between the two APRI frequencies were detected. A significantly positive relationship between stomatal conductance and root length density was found under APRI. Overall, alternate partial root-zone irrigation with high frequency has a great potential to promote root growth, expand water uptake capacity and reduce unproductive water loss in the arid apple production area.

Evaluation of tomato fruit quality response to water and nitrogen management under alternate partial root-zone irrigation

A pot experiment was conducted to investigate the effects of different water and nitrogen supply amounts on the comprehensive assessment of tomato fruit quality and root growth parameters under alternate partial root-zone irrigation.Three upper irrigation limitations(i.e.70%(W1),80%(W2)and 90%(W3)of field capacity,respectively)and three N-fertilizer levels(i.e.0.18(N1),0.30(N2)and 0.42(N3)g/kg soil,respectively)were arranged with a randomized complete block design,and alternate partial root-zone irrigation method was applied.Results showed that fruit yields under deficit irrigation(W1 and W2)were decreased by 6.9%and 2.0%respectively compared with W3 under N1 level.Yields of tomato under W1N1 and W1N2 combinations were also reduced by 10.3%and 7.2%,respectively compared with W1N3 combination.Root dry weight,root length,root surface area and root volume were all increased in W1N2 treatment.According to two-way ANOVA,the root parameters except root dry weight,were extremely sensitive to water,nitrogen and the cross effect of the two factors.TSS(total soluble solids),SS(soluble sugars)and OA(organic acid)in the fruits increased with the decrease in irrigation water,OA and NC reduced with decreasing amount of nitrogen.Moreover,within an appropriate range,as more irrigation water and nitrogen were applied,the higher VC(vitamin C)and lycopene contents were identified in the fruits.Eventually,the combinational evaluation method(i.e.entropy method and gray relational analysis)showed that W2N2 ranked highest in comprehensive fruit quality.Therefore,considering the tradeoff between fruit comprehensive quality and yields,upper irrigation limitation of 80%θf and N-fertilizer of 0.30 g/kg soil with alternate partial root-zone irrigation was the optimal cultivation strategy for the greenhouse tomato in autumn-winter season in northwest China.

Biochar and alternate partial root-zone irrigation greatly enhance the effectiveness of mulberry in remediating lead-contaminated soils

Aims Soil lead contamination has become increasingly serious and phytoremediation can provide an effective way to reclaim the contaminated soils.This study aims to examine the growth,lead resistance and lead accumulation of mulberry(Morus alba L.)seedlings at four levels of soil lead contamination with or without biochar addition under normal or alternative partial root-zone irrigation(APRI).Methods We conducted a three-factor greenhouse experiment with biochar(with vs.without biochar addition),irrigation method(APRI vs.normal irrigation)and four levels of soil lead(0,50,200 and 800 mg·kg^(-1)).The performance of the seedlings under different treatments was evaluated by measuring growth traits,osmotic substances,antioxidant enzymes and lead accumulation and translocation.Important Findings The results reveal that mulberry had a strong ability to acclimate to soil lead contamination,and that biochar and APRI synergistically increased the biomass and surface area of absorption root across all levels of soil lead.The seedlings were able to resist the severe soil lead contamination(800 mg·kg^(-1) Pb)by adjusting glutathione metabolism,and enhancing the osmotic and oxidative regulating capacity via increasing proline content and the peroxidase activity.Lead ions in the seedlings were primarily concentrated in roots and exhibited a dose–effect associated with the lead concentration in the soil.Pb,biochar and ARPI interactively affected Pb concentrations in leaves and roots,translocation factor and bioconcentration.Our results suggest that planting mulberry trees in combination with biochar addition and APRI can be used to effectively remediate lead-contaminated soils.

Effects of Alternative Partial Root-zone Irrigation and Nitrogen Fertilizer on Plukenetia volubilis Seedlings

This study was aimed to investigate the effects of alternative partial rootzone irrigation and nitrogen fertilizer on the potted seedlings of Plukenetia volubilis.A total of 7 treatments were designed with three factors, i.e., irrigation amount, irrigation mode and nitrogen fertilizer. The growth, photosynthesis and water use efficiency were analyzed. The results showed that compared with those under full irrigation, the biomass and water consumption under alternative partial root-zone irrigation were reduced by 5% and 75%, respectively, and the water use efficiency was increased by 60%. Under severe drought conditions, the root cap ratio in the nitrogen fertilizer treatment group was increased by 30%; the leaf area index, photosynthetic rate and biomass under alternative partial root-zone irrigation were reduced by 38%, 9% and 18%, respectively. It indicates that under severe drought conditions, alternative partial root-zone irrigation is not suitable to be matched with application of nitrogen fertilizer. In short, under moderate drought conditions, alternative partial root-zone irrigation could reduce transpiration and improve water use efficiency, and it is an effective water-saving irrigation technology for the plantation of P.volubilis plants.

Effect of Partial Root-Zone Irrigating Deuterium Oxide on the Properties of Water Transportation and Distribution in Young Apple Trees

Partial root-zone irrigation （PRI） has been proved to be an optimal water-saving irrigation technology, however, few studies were done on water transportation and distribution under PRI. The present study was performed to investigate the water transportation and distribution among the wet and dry root-zones and the shoot using deuterium water （D2O） in 1/4 root-zone PRI experiment. It also aimed to determine and analyze the D2O relative abundance within different types of roots and shoots. The results indicated that water could be transported from roots in wet root-zone to roots in dry root-zone and shoots within 2 h after irrigation. Water transportation in roots of wet-zone was carried out by absorbing root, 1-2 mm root, 2-5 mm root, and〉5 mm root progressively, while through a reverse process in three dry root-zones. In shoots, water was transported to trunk, central trunk, annual branches, shoot and leaf progressively. Thus in the young apple trees subjected to PRI, water was distributed ifrst in the roots, including the roots in the wet and dry root-zones, to satisfy the water need of roots itself, and then transported to the shoot within hours of irrigation.

Wetting alternating with partial drying during grain filling increases lysine biosynthesis in inferior rice grain

Lysine content is a criterion of the nutritional quality of rice.Understanding the process of lysine biosynthesis in early-flowering superior grain(SG)and late-flowering inferior grain(IG)of rice would advance breeding and cultivation to improve nutritional quality.However,little information is available on differences in lysine anabolism between SG and IG and the underlying mechanism,and whether and how irrigation regimes affect lysine anabolism in these grains.A japonica rice cultivar was grown in the field and two irrigation regimes,continuous flooding(CF)and wetting alternating with partial drying(WAPD),were imposed from heading to the mature stage.Lysine content and activities of key enzymes of lysine biosynthesis,and levels of brassinosteroids(BRs)were lower in the IG than in the SG at the early grainfilling stage but higher at middle and late grain-filling stages.WAPD increased activities of these key enzymes,BR levels,and contents of lysine and total amino acids in IG,but not SG relative to CF.Application of 2,4-epibrassinolide to rice panicles in CF during early grain filling reproduced the effects of WAPD,but neither treatment altered the activities of enzymes responsible for lysine catabolism in either SG or IG.WAPD and elevated BR levels during grain filling increased lysine biosynthesis in IG.Improvement in lysine biosynthesis in rice should focus on IG.

Yield Response to Deficit Irrigation and Partial Root-Zone Drying in Processing Tomato （Lycopersicon esculentum Mill,）

Due to the global expansion of irrigated areas and the limited availability of irrigation water, it is necessary to optimize water use in order to maximize crop yields under water deficit conditions. To evaluate the yield response of two processing tomato hybrids （Ercole and Genius） grown under different irrigation treatments, a field trial was conducted during the 2008 growing season in Southern Italy. Three irrigation treatments were used： the restitution of 70% of maximum evapotranspiration （ETc） both under ＂Deficit Irrigation＂ （70DI） and ＂Partial Root-zone Drying＂ （70PRD） strategies; full irrigated （FI： 100% ETc）. The two water deficit irrigation treatments （DI and PRD） showed stomatal conductance values lower than FI treatment and saved a substantial amount of water maintaining reasonable marketable yield. Moreover, PRD strategy showed slightly higher ＂Water Use Efficiency＂ （WUE） values than DI. Finally, ＂yield response factor＂ （Ky） showed always values less than unity, indicating the possibility to adopt, within certain limited condition, both DI and PRD in field-grown processing tomato cultivated in Southern Italy. In conclusion, in our experimental conditions, deficit irrigation practices seem to be acceptable relatively to processing tomato yield aspects and Ky could be promoted as a useful indicator for irrigation in water deficit conditions.

Stem flow of seed-maize under alternate furrow irrigation and double-row ridge planting in an arid region of Northwest China

Maize is widely planted throughout the world and has the highest yield of all the cereal crops. The arid region of North- west China has become the largest base for seed-maize production, but water shortage is the bottleneck for its long-term sustainability. Investigating the transpiration of seed-maize plants will offer valuable information for suitable planting and irrigation strategies in this arid area. In this study, stem flow was measured using a heat balance method under alternate furrow irrigation and double-row ridge planting. Meteorological factors, soil water content （e）, soil temperature （Ts） and leaf area （LA） were also monitored during 2012 and 2013. The diurnal stem flow and seasonal dynamics of maize plants in the zones of south side female parent （SFP）, north side female parent （NFP） and male parent （MP） were investigated. The order of stem flow rate was： SFP〉MP〉NFP. The relationships between stem flow and influential factors during three growth stages at different time scales were analyzed. On an hourly scale, solar radiation （Rs） was the main driving factor of stem flow. The influence of air temperature （Ta） during the maturity stage was significantly higher than in other periods. On a daily scale, Rs was the main driving factor of stem flow during the heading stage. During the filling growth stage, the main driving factor of NFP and MP stem flow was RH and Ts, respectively. However, during the maturity stage, the environ- mental factors had no significant influence on seed-maize stem flow. For different seed-maize plants, the main influential factors were different in each of the three growing seasons. Therefore, we identified them to accurately model the FP and MP stem flow and applied precision irrigation under alternate partial root-zone furrow irrigation to analyze major factors affecting stem flow in different scales.

Alternate Furrow Irrigation: A Practical Way to Improve Grape Quality and Water Use Efficiency in Arid Northwest China

Field experiments were conducted for two years to investigate the benefits of alternate furrow irrigation on fruit yield, quality and water use efficiency of grape （Vitis vinifera L. cv. Rizamat） in the arid region of Northwest China. Two irrigation treatments were included, i.e., conventional furrow irrigation （CFI, two root-zones were simultaneously irrigated during the consecutive irrigation） and alternate partial root-zone furrow irrigation （AFI, two root-zones were alternatively irrigated during the consecutive irrigation）. Results indicate that AFI maintained similar photosynthetic rate （Pn） but with a reduced transpiration rate when compared to CFI. As a consequence, AFI improved water use efficiency based on evapotranspiration （WUEEr, fruit yield over water consumed） and irrigation （WUE~, fruit yield over water irrigated） by 30.0 and 34.5%, respectively in 2005, and by 12.7 and 17.7%, respectively in 2006. AFI also increased the edible percentage of berry by 2.91-4.79% significantly in both years. Vitamin C （Vc） content content of berry was increased by 25.6-37.5%, and tritrated acidity （TA） was reduced by 9.5-18.1% in AFI. This resulted in an increased total soluble solid content （TSS） to TA ratio （TSS/TA） by 11.5-16.7% when compared to CFI in both years. Our results indicate that alternate furrow irrigation is a practical way to improve grape fruit quality and water use efficiency for irrigated crops in arid areas.

Role of alternate and fixed partial root-zone drying on water use efficiency and growth of maize(Zea mays L.)in gypsiferous soils

Alternate partial root-zone drying(APRD)is a water-saving method but can regulate crop physiological responses.A pot experiment has been conducted to study the efficiency of partial and fixed root-zone drying on the growth and production of maize(Zea mays L)in addition to the water use efficiency in soils with different gypsum content.The experimental treatments include three irrigation treatments,i.e.Conventional Irrigation(CI),Alternate Partial Root-zone Drying(APRD)and Fixed Partial Root-zone Drying(FPRD),and three soils with different gypsum content"(60.0[G1],153.7[G2],and 314.2[G3]g kg^(-1))".The vegetative growth,root dry mass and physiological indices(leaf relative water content,carotenoid concentration,proline)have been studied during three stages of maize plant growth(jointing,tasselling,and maturing).The Results showed that compared to CI,APRD and FPRD increased water use efficiency by 38.93 and 14.94%based on dry seed yield.In addition,compared to CI,APRD increased maize seed yield by 4.62-20.71%,while FPRD decreased yield by 19.24-5.28%for the gypsiferous soils G2 and G3,respectively.APRD has a slight effect on leaf water potential,leaf relative water content,carotenoid and proline activities from jointing to maturing stages at the three gypsiferous soils.Results suggest that APRD could make maize plants use water even more productively with better adaptation to water shortages in the gypsiferous soils.

Study of partial discharge characteristics in HFO-1234ze(E)/N2 mixtures

HFO-1234 ze(E)(trans-1,3,3,3-tetrafluoropropene,chemical formula:C3H2F4)is an extremely environmentally friendly SF6 alternative gas with high electrical strength.In this paper,the partial discharge(PD)characteristics of HFO-1234 ze(E)/N2 mixtures were studied using the gas insulation test platform.The PD inception voltage of insulating gas under positive and negative half cycles of power frequency was tested.Using SF6/N2 mixtures as a control group,the effects of electrode spacing,mixing ratio and pressure on the insulation performance of HFO-1234 ze(E)/N2 mixtures were explored.The test results show that the PD inception voltage of the negative half-cycle of pure HFO-1234 ze(E)under short electrode spacing can reach 0.96-1.04 times of pure SF6 under different pressures;the PD inception voltage of40%HFO-1234 ze(E)/60%N2 mixtures at 0.3 MPa is 0.67-0.89 times that of SF6/N2 mixtures under the same conditions,which has great application prospect.

Effects of packing particles on the partial discharge behavior and the electrical characterization of oxygen PBRs

Packed-bed reactors(PBRs)hold great promise for environmental applications,but a deeper understanding of the behavior of plasma discharge within PBRs is required.To this end,a partial-discharge alternative equivalent circuit for PBRs was established in this work.Dielectric particles(glass beads or glass sand)were used to place focus on the effects of the particle size and shape on the partial discharge behavior of the oxygen PBRs.Some electrical characterizations were explored(e.g.the effective dielectric capacitance,partial discharge coefficient,and corrected burning voltage)that may differ from long-standing interpretations.The findings indicate that the suppressive effect of surface discharge on filament discharge is stronger with the decrease of the particle size.For partial discharge,the effective dielectric capacitance is always less than the dielectric capacitance.The corrected burning voltage and partial discharge tendency increase with the decrease of the particle size.As compared to an empty reactor,the average electric field in the PBR was found to be improved by 3–4 times,and the ozone energy efficiency and production were promoted by more than 20%and 15%,respectively.The plasma processing capacity can therefore be improved by choosing a relatively large size or a complex,irregularly-shaped packing material that is suitable for the discharge gap.

Crank-Nicolson ADI Galerkin Finite Element Methods for Two Classes of Riesz Space Fractional Partial Differential Equations

In this paper,two classes of Riesz space fractional partial differential equations including space-fractional and space-time-fractional ones are considered.These two models can be regarded as the generalization of the classical wave equation in two space dimensions.Combining with the Crank-Nicolson method in temporal direction,efficient alternating direction implicit Galerkin finite element methods for solving these two fractional models are developed,respectively.The corresponding stability and convergence analysis of the numerical methods are discussed.Numerical results are provided to verify the theoretical analysis.

不同灌溉方式和灌水量对土壤水盐及燕麦生长特征的影响

[目的]探究不同灌溉方式和灌水量对土壤水盐变化规律及燕麦生长特征的影响,为提高盐碱地作物的生产效能和土壤水分管理提供科学参考。[方法]采用盆栽试验,设置3种灌溉方式:常规灌溉、固定单侧灌溉(fixed unilateral root zone irrigation,FURI)、交替灌溉(alternative partial root zone irrigation,APRI),3组灌水量:W_1(60%θ_f~70%θ_f,θ_f为田间持水率),W_2(70%θ_f~80%θ_f)和W_3(80%θ_f~90%θ_f),以常规灌溉作为对照,共9组交互处理。[结果](1)不同灌溉方式下,土壤各层含水率变化趋势基本一致,随灌水量增加洗盐效果越显著,常规灌溉的深层含水率总体高于其他两种灌溉方式。(2)燕麦株高、叶绿素相对含量(relative chlorophyll content of leaves,SPAD)、品质随灌水量的增加而上升,与常规灌溉相比,W_2灌溉水平下,分根交替灌溉处理的粗脂肪,粗蛋白,β-葡聚糖含量分别增加7.02%,3.76%,6.06%,但降低了燕麦叶片的SPAD值,影响其光合能力。(3)随着燕麦生育期的推进,土壤盐分均呈现不同程度的累积,分根交替灌溉的积盐率最低,同时对燕麦根系生长、水分利用效率及产量影响显著,其中根系总长、根系总表面积、根系总体积较相同灌水量(W_2)的常规灌溉分别增加6.75%,6.92%,12.5%,水分利用效率提高17.32%。[结论]采用分根交替灌溉方式下的中等灌水量(W_2)有利于提高燕麦的生产效能,对盐分累积的控制效果较好。

分根区交替灌溉在农业生产中的应用研究进展

在全球水资源日益紧缺的背景下,如何提高农业用水利用效率,对于农业可持续发展具有重要意义。分根区交替灌溉作为一种具有较好前景的高效节水灌溉技术,被认为是未来最具发展潜力的灌溉技术之一。目前,各国科技工作者围绕分根区交替灌溉对作物生长、生理的影响方面开展了大量研究,并对其节水机制进行了解析,亟待总结梳理。本文回顾了分根区交替灌溉技术的发展历程,综述了分根区交替灌溉对作物生长发育、产量及品质的影响,归纳了该技术影响作物水分利用效率的生理机制,并对分根区交替灌溉在未来的研究方向进行了展望,以期为作物分根区交替灌溉节水灌溉机理研究和节水灌溉技术的革新提供参考。

果树根系分区交替灌溉研究进展

通过对国内外分根区交替灌溉研究成果分析,综述了一种既能满足果树水分需求,又能控制植株长势、减少“奢侈”蒸腾、改善果实品质的果园节水灌溉新技术——果树根系分区交替灌溉,阐明了其理论体系和节水优产机理,概述了与该技术有关的部分根区干燥技术研究现状和存在的问题,提出了几种适合在果园应用的根系分区交替灌溉技术实施模式并对其可行性进行了探讨。分析了果树根系分区交替灌溉技术的应用前景和需要进一步研究的问题。

番茄垂向分根区交替控制滴灌室内试验及节水机理

开展了垂向分根区交替控制滴灌番茄试验研究,设中等水分处理和低水分处理的垂向分根区交替控制滴灌、中等水分处理的表面滴灌3个处理。结果表明:垂向分根区交替控制滴灌可以在作物上下根区层形成干湿交替的区域,在适宜水分条件下能够大大降低耗水强度,使耗水过程趋于平缓,有利于控制植株长势、壮大径杆直径,以及增加下层根系的比重与根冠比,番茄产量在无显著下降的情况下可实现节水46.5%。在较高的土壤水分下限条件下适时进行番茄垂向分根区交替控制滴灌能够有效降低设施农业的运行管理费用、增加番茄商品率及实现节约水资源的目的。

根系分区交替滴灌对棉花产量和水分利用效率的影响

为了探讨旱区农业节水新途径,在甘肃省石羊河流域下游荒漠绿洲区采用常规滴灌和分根区交替滴灌方式研究不同灌溉方式对大田棉花生长发育、产量、水分利用效率以及土壤水分分布的影响。结果表明,交替滴灌处理棉花叶片气孔开度减小,减少了奢侈的蒸腾损失,灌溉定额较小时根系分区交替滴灌对棉花的株高抑制作用较明显,灌水定额较大时限制作用不显著。根系分区交替滴灌技术在大田条件下可使籽棉产量比常规滴灌处理提高21.1%,总水分利用效率和灌溉水利用效率分别提高17.9%和20.9%。同等产量水平下与常规滴灌相比,交替滴灌可节省30.8%的灌水量。本研究表明根系分区交替滴灌是一种切实可行的节水灌溉技术,可在干旱缺水的棉花生产地区进一步研究和推广应用。