Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata.Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus(TMV)by the co...Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata.Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus(TMV)by the conventional halfleaf method.Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by western-blot and RT-PCR analysis.The tested compounds showed moderate inactivation effect,whereas the lycoricidine derivatives showed good protective effect.The protective inhibitory activity of compounds L1(N-methyl-2,3,4-trimethoxyly-coricidine)(60.8%)and L3(N-methyl-2-methoxy-3,4-acetonidelycoricidine)(62.0%)was almost similar to the positive control,Ningnanmycin(66.4%).RT-PCR and Western-blot analysis displayed that compounds L1,L3,L5(N-allyl-2,3,4-triallyloxylycoricidine)exhibited antiviral activity,which was evidenced by reducing TMV-CP gene replication and TMV-CP protein expression.Additionally,defensive enzyme activities confirmed that compound L1 could increase the activity of PAL,POD,SOD to improve disease resistance of tobacco.展开更多
Objective To study the benzylphenethylamine alkaloids from the bulbs and flowers of Lycoris radiata.Methods Alkaloids were isolated by various column chromatographic methods and their structures were identified by spe...Objective To study the benzylphenethylamine alkaloids from the bulbs and flowers of Lycoris radiata.Methods Alkaloids were isolated by various column chromatographic methods and their structures were identified by spectral data.Results Fifteen known benzylphenethylamine alkaloids were isolated and identified as lycoramine(1),O-demethyllycoramine(2),N-demethyllycoramine(3),galanthamine(4),lycorine(5),caranine(6),ungminorine(7),narciclasine(8),5-hydroxy-10-O-demethyl-homolycorine(9),hippeastrine(10),ungerine(11),hippeastrine N-oxide (12),O-demethylhaemanthamine(13),haemanthidine(14),and 8-demethoxyhostasine(15).Conclusion Compound 15 is first isolated from the plants in Amaryllidaceae,compounds 3,6,9,and 11 are first reported from the plants in Lycoris Herb.,and compounds 2,7,and 14 are isolated from L.radiata for the first time.The1 3C-NMR data of compouds 3,7,and 12 are first reported in the present study.Furthermore,the galasine-type alkaloid is isolated from the plants of Lycoris Herb.for the first time.展开更多
基金the National Natural Science.Foundation of China(Nos.31770389,31470427,31270404).
文摘Fifteen known amaryllidaceae alkaloids were isolated from the bulbs of Lycoris radiata.Some of the compounds and lycoricidine derivatives had been screened for the activities against tobacco mosaic virus(TMV)by the conventional halfleaf method.Lycoricidine derivatives were also carried out the assay of effect on systemic infection of TMV by western-blot and RT-PCR analysis.The tested compounds showed moderate inactivation effect,whereas the lycoricidine derivatives showed good protective effect.The protective inhibitory activity of compounds L1(N-methyl-2,3,4-trimethoxyly-coricidine)(60.8%)and L3(N-methyl-2-methoxy-3,4-acetonidelycoricidine)(62.0%)was almost similar to the positive control,Ningnanmycin(66.4%).RT-PCR and Western-blot analysis displayed that compounds L1,L3,L5(N-allyl-2,3,4-triallyloxylycoricidine)exhibited antiviral activity,which was evidenced by reducing TMV-CP gene replication and TMV-CP protein expression.Additionally,defensive enzyme activities confirmed that compound L1 could increase the activity of PAL,POD,SOD to improve disease resistance of tobacco.
基金National Natural Science Foundation of China(20972166&31070288)Ministry of Education of China through its 985 and 111 projects(MUC 98503-001006&B08044)
文摘Objective To study the benzylphenethylamine alkaloids from the bulbs and flowers of Lycoris radiata.Methods Alkaloids were isolated by various column chromatographic methods and their structures were identified by spectral data.Results Fifteen known benzylphenethylamine alkaloids were isolated and identified as lycoramine(1),O-demethyllycoramine(2),N-demethyllycoramine(3),galanthamine(4),lycorine(5),caranine(6),ungminorine(7),narciclasine(8),5-hydroxy-10-O-demethyl-homolycorine(9),hippeastrine(10),ungerine(11),hippeastrine N-oxide (12),O-demethylhaemanthamine(13),haemanthidine(14),and 8-demethoxyhostasine(15).Conclusion Compound 15 is first isolated from the plants in Amaryllidaceae,compounds 3,6,9,and 11 are first reported from the plants in Lycoris Herb.,and compounds 2,7,and 14 are isolated from L.radiata for the first time.The1 3C-NMR data of compouds 3,7,and 12 are first reported in the present study.Furthermore,the galasine-type alkaloid is isolated from the plants of Lycoris Herb.for the first time.
