Impact of Long-Term Fertilization on Community Structure of Ammonia Oxidizing and Denitrifying Bacteria Based on amoA and nirK Genes in a Rice Paddy from Tai Lake Region,China

Ammonia oxidizing （AOB） and denitrifying bacteria （DNB） play an important role in soil nitrogen transformation in natural and agricultural ecosystems. Effects of long-term fertilization on abundance and community composition of AOB and DNB were studied with targeting ammonia monooxygenase （amoA） and nitrite reductase （nirK） genes using polymerase chain reaction- denaturing gradient gel electrophoresis （PCR-DGGE） and real-time PCR, respectively. A field trial with different fertilization treatments in a rice paddy from Tai Lake region, centre East China was used in this study, including no fertilizer application （NF）, balanced chemical fertilizers （CF）, combined organic/inorganic fertilizer of balanced chemical fertilizers plus pig manure （CFM）, and plus rice straw return （CFS）. The abundances and riehnesses of amoA and nirK were increased in CF, CFM and CFS compared to NF. Principle component analysis of DGGE profiles showed significant difference in nirK and amoA genes composition between organic amended （CFS and CFM） and the non-organic amended （CF and NF） plots. Number of amoA copies was significantly positively correlated with normalized soil nutrient richness （NSNR） of soil organic carbon （SOC） and total nitrogen （T-N）, and that of nirK copies was with NSNR of SOC, T-N plus total phosphorus. Moreover, nitrification potential showed a positive correlation with SOC content, while a significantly lower denitrification potential was found under CFM compared to under CFS. Therefore, SOC accumulation accompanied with soil nutrient richness under long-term balanced and organic/inorganic combined fertilization promoted abundance and diversity of AOB and DNB in the rice paddy.

Distribution characteristics of ammonia-oxidizing bacteria in the Typha latifolia constructed wetlands using fluorescent in situ hybridization(FISH)

A molecular biology method, fluorescent in situ hybridization（FISH）, in which the pre-treatment was improved in allusion to the media of the constructed wetlands（CW）, e.g. the soil and the grit, was used to investigate the vertical distribution characteristics of ammonia-oxidizing bacteria（AOB） quantity and the relation with oxidation-reduction potential（ORP） in the Typha latifolia constructed wetlands under three different Ioadings in summer from May to September. Results showed that the quantity of the AOB decreased in the Typha latifolia CW with the increase of vertical depth. However, the AOB quantity was 2-4 times the quantity of the control in the root area. Additionally, ORP in the rhizosphere was found to be higher than other areas, which showed that Typha latifolia CW was in an aerobic state in summer when using simulated non-point sewage at the rural area of Taihu Lake in China and small town combined sewage.

Variation of Potential Nitrification and Ammonia-Oxidizing Bacterial Community with Plant-Growing Period in Apple Orchard Soil

In this study, we investigated the potential nitrification and community structure of soil-based ammonia-oxidizing bacteria （AOB） in apple orchard soil during different growth periods and explored the effects of environmental factors on nitrification activity and AOB community composition in the soil of a Hanfu apple orchard, using a culture-dependent technique and denaturing gradient gel electrophoresis （DGGE）. We observed that nitrification activity and AOB abundance were the highest in November, lower in May, and the lowest in July. The results of statistical analysis indicated that total nitrogen （N） content, NH4＋-N content, NO3-N content, and pH showed significant correlations with AOB abundance and nitrification activity in soil. The Shannon-Winner diversity, as well as species richness and evenness indices （determined by PCR-DGGE banding patterns） in soil samples were the highest in September, but the lowest in July, when compared to additional sampled dates. The DGGE fingerprints of soil-based 16S rRNA genes in November were apparently distinct from those observed in May, July, and September, possessing the lowest species richness indices and the highest dominance indices among all four growth periods. Fourteen DGGE bands were excised for sequencing. The resulting analysis indicated that all AOB communities belonged to the 13-Proteobacteria phylum, with the dominant AOB showing high similarity to the Nitrosospira genus. Therefore, soil-based environmental factors, such as pH variation and content of NHa＋-N and NO3--N, can substantially influence the abundance of AOB communities in soil, and play a critical role in soil-based nitrification kinetics.

Community analysis of ammonia-oxidizing bacteria in activated sludge of eight wastewater treatment systems

We investigated the communities of ammonia-oxidizing bacteria （AOB） in activated sludge collected from eight wastewater treatment systems using polymerase chain reaction （PCR） followed by terminal restriction fragment length polymorphism （T-RFLP）, cloning, and sequencing of the α-subunit of the ammonia monooxygenase gene （amoA）. The T-RFLP fingerprint analyses showed that different wastewater treatment systems harbored distinct AOB communities. However, there was no remarkable difference among the AOB T- RFLP profiles from different parts of the same system. The T-RFLP fingerprints showed that a full-scale wastewater treatment plant （WWTP） contained a larger number of dominant AOB species than a pilot-scale reactor. The source of influent affected the AOB community, and the WWTPs treating domestic wastewater contained a higher AOB diversity than those receiving mixed domestic and industrial wastewater. However, the AOB community structure was little affected by the treatment process in this study. Phylogenetic analysis of the cloned amoA genes clearly indicated that all the dominant AOB in the systems was closely related to Nitrosomonas spp. not to Nitrosospira spp. Members of the Nitrosomonas oligotropha and Nitrosomonas communis clusters were found in all samples, while members of Nitrosomonas europaea cluster occurred in some systems.

Impact of acetochlor on ammonia-oxidizing bacteria in microcosm soils

Acetochlor is an increasingly used herbicide on corn in North China. Currently, the effect of acetochlor on soil ammonia-oxidizing bacteria (AOB) communities is not well documented. Here, we studied the diversity and community composition of AOB in soil amended with three concentrations of acetochlor (50, 150, 250 mg/kg) and the control (0 mg acetochlor/kg soil) in a microcosm experiment by PCR-DGGE (polymerase chain reaction-denaturing gradient gel electrophoresis) and the phylogenetic analysis of excised ...

Effect of hydraulic retention time and pH on oxidation of ferrous iron in simulated ferruginous acid mine drainage treatment with inoculation of iron-oxidizing bacteria

The effect of hydraulic retention time (HRT) and pH on the biooxidation of ferrous iron during simulated acid mine drainage (AMD) treatment was investigated.The simulated AMD was highly acidic (pH 2.5), rich in iron (about 1700 mg/L) and copper (about 200 mg/L), and contained high concentrations of sulfate (about 4700 mg/L).The biooxidation of ferrous iron was studied in a laboratory-scale upflow packed bed bioreactor (PBR).The HRT was shortened stepwise from 40 h to 20 h, 13 h, and 8 h under the acidic environment at a pH value of 2.2.Then, the influent pH value was changed from 2.2 to 1.2 at a constant suitable HRT.Physiochemical and microbial community structure analyses were performed on water samples and stuffing collected from the bioreactor under different conditions.The results indicate that the efficiency of ferrous iron oxidation gradually decreased with the decrease of HRT, and when the HRT exceeded 13 h, ferrous iron in AMD was almost completely oxidized.In addition, the best efficiency of ferrous iron oxidation was achieved at the influent pH value of 1.8.Microbial community structure analyses show that Leptospirillum is the predominant genus attached in the bioreactor, and low influent pH values are suitable for the growth of Leptospirillum.

Corrosion and Electrochemical Behavior of 316L Stainless Steel in Sulfate-reducing and Iron-oxidizing Bacteria Solutions

Corrosion and electrochemical behavior of 316L stainless steel was investigated in the presence of aerobic iron-oxidizing bacteria IOB and anaerobic sulfate-reducing bacteria SRB isolated from cooling water systems in an oil refinery using electrochemical measurement, scanning electron microscopy SEM and energy dispersive atom X-ray analysisEDAX. The results show the corrosion potential and pitting potential of 316L stainless steel decrease distinctly in the presence of bacteria, in comparison with those observed in sterile medium under the same exposure time. SEM morphologies have shown that 316L stainless steel reveals no signs of pitting attack in the sterile medium. However, micrometer-scale corrosion pits were observed on 316L stainless steel sur- face in the presence of bacteria. The presence of SRB leads to higher corrosion rates than IOB. The interactions between the stainless steel surface, abiotic corrosion products, and bacterial cells and their metabolic products in- creased the corrosion damage degree of the passive film and accelerated pitting propagation.

Se-Bearing Colloidal Particles Produced by Sulfate-Reducing Bacteria and Sulfide-Oxidizing Bacteria: TEM Study

As determined by transmission electron microscopy (TEM), the reduction of selenate and selenite by Desulfovibrio desulfuricans, a sulfate-reducing bacterium, produces spherical (Se, S) sub-micro particles outside the cell. The particles are crystalline or amorphous, depending on medium composition. Amorphous-like Se-rich spherical particles may also occur inside the bacterial cells. The bacteria are more active in the reduction of selenite than selenate. The Desulfovibrio desulfuricans bacterium is able to extract S in the (S, Se) solid solution particles and transform S-rich particles into Se-rich and Se crystals. Photoautotrophs, such as Chromatium spp., are able to oxidize sulfide (S2-). When the bacteria grow in sulfide- and selenide-bearing environments, they produce amorphous-like (S, Se) globules inside the cells. TEM results show that compositional zonation in the (S, Se) globules occur in Chromatium spp. collected from a top sediment layer of a Se-contaminated pond. S2-?may be from the products of sulfate-reducing bacteria. Both the sulfate-reducing bacteria and photosynthetic Chromatium metabolize S preferentially over Se. It is proposed that the S-rich zones are formed during photosynthesis (day) period, and the Se-rich zones are formed during respiration active (night) period. The results indicate that both Desulfovibrio desulfuricans and Chromatium spp. are able to immobilize the oxidized selenium (selenate and/or selenite) in the forms of elemental selenium and (Se, S) solid solutions. The bacteria reduce S in the (Se, S) particles and further enrich Se in the crystalline particles. The reduced S combines with Fe2+ to form amorphous FeS.

The in situ spectral methods for examining redox status of c-type cytochromes in metal-reducing/oxidizing bacteria

The membrane-associated c-type cytochromes(c-Cyts) have been well known as the key enzymes mediating extracellular electron transfer to terminal electron acceptors, resulting in biogeochemical elemental transformation, contaminant degradation, and nutrient cycling. Although c-Cyts-mediated metal reduction or oxidation have been mainly investigated with the purified proteins of metal reducing/oxidizing bacteria, the in vivo behavior of c-Cyts is still unclear, given the difficulty in measuring the proteins of intact cells. Fortunately, the in situ spectroscopy would be ideal for measuring the reaction kinetics of c-Cyts in intact cells under noninvasive physiological conditions. It can also help the establishment of kinetic/thermodynamic models of extracellular electron transfer processes, which are essential to understand the electron transfer mechanisms at the molecular scale. This review briefly summarizes the current advances in spectral methods for examining the c-Cyts in intact cells of dissimilatory metal reducing bacteria and Fe(Ⅱ)-oxidizing bacteria.

In vitro establishment of shoot meristems of Ilex paraguariensis and identification of endophytic bacteria

Yerba mate(Ilex paraguariensis A.St.-Hil.)is a species of great economic,social and environmental importance for the southern regions of Brazil,Uruguay and Argentina.Currently the most diverse products are obtained from mate leaves,including mate tea.The objective of this study was to establish shoot meristem cultures(meristematic dome and a few primordia)of elite clones and identify the endophytic bacteria present in the explants.We tested the effect of clones(F1,F2,A03 and A07),culture media(MS,1/2MS,1/4MS and WPM),cytokinins(kinetin,BA and 2iP),activated charcoal(1,2 and 3 g L^-1),and disinfecting agent(sodium hypochlorite and mercuric chloride)on in vitro establishment.F1 and F2 clones were the most responsive for shoot meristem in vitro culture.WPM medium supplemented with 8.8 lM 2iP,0.2 lM NAA and 3 g L^-1 activated charcoal was the most suitable for the in vitro establishment of the F1 clone.No phytotoxic effect of the disinfectant was observed and some meristems sprouted.The isolated endophytic bacterium was identified for the first time in yerba mate as Agrobacterium larrymoorei.To conclude,we were able to establish in vitro culture of yerba mate using meristems as explants but the tissues were not free of endophytic microorganisms which could interfere with explant response.

Optimization of Fermentation Media for Enhancing Nitrite-oxidizing Activity by Artificial Neural Network Coupling Genetic Algorithm

Two artificial intelligence techniques, artificial neural network and genetic algorithm, were applied to optimize the fermentation medium for improving the nitrite oxidization rate of nitrite oxidizing bacteria. Experiments were conducted with the composition of medium components obtained by genetic algorithm, and the experimental data were used to build a BP (back propagation) neural network model. The concentrations of six medium components were used as input vectors, and the nitrite oxidization rate was used as output vector of the model. The BP neural network model was used as the objective function of genetic algorithm to find the optimum medium composition for the maximum nitrite oxidization rate. The maximum nitrite oxidization rate was 0.952 g 2 NO-2-N·(g MLSS)-1·d-1 , obtained at the genetic algorithm optimized concentration of medium components (g·L-1 ): NaCl 0.58, MgSO 4 ·7H 2 O 0.14, FeSO 4 ·7H 2 O 0.141, KH 2 PO 4 0.8485, NaNO 2 2.52, and NaHCO 3 3.613. Validation experiments suggest that the experimental results are consistent with the best result predicted by the model. A scale-up experiment shows that the nitrite degraded completely after 34 h when cultured in the optimum medium, which is 10 h less than that cultured in the initial medium.

The effect of soil moisture on the response by fungi and bacteria to nitrogen additions for N_(2)O production

In addition to bacteria,the contribution of fungi to nitrous oxide(N_(2)O)production has been recognized but the responses of these two broad and unrelated groups of microorganisms to global environmental changes,atmospheric nitrogen(N)deposition,and precipitation in terms of N_(2)O production are unclear.We studied how these two microbial-mediated N_(2)O production pathways responded to soil moisture conditions and to N addition in an N-limited temperate forest.Soils from a long-term N addition experiment in Changbai Mountain,northeastern China were incubated.Varied concentrations of cycloheximide and streptomycin,both inhibitors of fungal and bacterial activity,were used to determine the contributions of both to N_(2)O production in 66%,98%and 130%water-filled pore spaces(WFPS).The results showed that N_(2)O production decreased significantly with increasing cycloheximide concentration whereas streptomycin was only inhibiting N_(2)O emissions at 98%and 130%WFPS.The bacterial pathway of N_(2)O production in N-addition(Nadd)soil was significantly more dominant than that in untreated(Namb)soil.The difference in the fungal pathway of N_(2)O production between the soil with nitrogen addition and the untreated soil was not significant.Net N_(2)O emissions increased with increasing soil moisture,especially at 130%WFPS,a completely flooded condition.Bacteria dominated carbon dioxide(CO_(2))and N_(2)O emissions in Nadd soil and at 130%WFPS regardless of N status,while fungi dominated CO_(2)and N_(2)O emissions in soil without N addition at 66%and 98%WFPS.The results suggest that flooded soil is an important source of N_(2)O emissions and that bacteria might be better adapted to compete in fertile soils under anoxic conditions.

Antibacterial activity of bioceramic coatings on Mg and its alloys created by plasma electrolytic oxidation(PEO): A review

Mg and its alloys are suitable choices for implant materials due to their biodegradability and biocompatibility features. However, the high electrochemical activity of this family of biomaterials results in their fast degradation and severe corrosion in the physiological environment,producing hydrogen(H;) gas, and therefore increasing the p H of the environment. To meet the clinical requirements, the degradation rate of Mg biomaterials needs to be reduced. Nevertheless, higher corrosion resistance of Mg results in a low alkaline p H, weakening the antibacterial activity. Therefore, while the rapid degradation problem of Mg-based biomaterials needs to be addressed, good antibacterial properties are also necessary. By using the plasma electrolytic oxidation(PEO) surface modification technique, the antibacterial activity of Mg and its alloys can be enhanced while maintaining their corrosion protection properties at a high level. Throughout the PEO process, introducing antibacterial agents into solutions results in a major increase in antibacterial activity of the coatings. Moreover, post-or pre-processing on PEO coatings can provide better protection against bacteria. In this review, the antibacterial activity of PEO coatings applied on Mg and also its alloys will be discussed in more detail.

Distinct influence of trimethylamine N-oxide and high hydrostatic pressure on community structure and culturable deep-sea bacteria

Trimethylamine N-oxide(TMAO)is one of the most important nutrients for bacteria in the deep-sea environment and is capable of improving pressure tolerance of certain bacterial strains.To assess the impact of TMAO on marine microorganisms,especially those dwelling in the deep-sea environment,we analyzed the bacterial community structure of deep-sea sediments after incubated under different conditions.Enrichments at 50 MPa and 0.1 MPa revealed that TMAO imposed a greater influence on bacterial diversity and community composition at atmospheric pressure condition than that under high hydrostatic pressure(HHP).We found that pressure was the primary factor that determines the bacterial community.Meanwhile,in total,238 bacterial strains were isolated from the enrichments,including 112 strains a ffiliated to 16 genera of 4 phyla from the Yap Trench and 126 strains a ffiliated to 11 genera of 2 phyla from the Mariana Trench.Treatment of HHP reduced both abundance and diversity of isolates,while the presence of TMAO mainly af fected the diversity of isolates obtained.In addition,certain genera were isolated only when TMAO was supplemented.Taken together,we demonstrated that pressure primarily defines the bacterial community and culturable bacterial isolates.Furthermore,we showed for the first time that TMAO had distinct influences on bacterial community depending on the pressure condition.The results enriched the understanding of the significance of TMAO in bacterial adaptation to the deep-sea environment.

Effect of Bifidobacterium triple live bacteria on inflammatory factors, oxidative stress and T lymphocyte subsets in patients with ulcerative colitis

Objective: To explore the effect of Bifidobacterium triple live bacteria on inflammatory factors, oxidative stress and T lymphocyte subsets in patients with ulcerative colitis (UC). Method: A total of 90 patients with UC treated in our hospital were selected and randomly divided into the observation group and the control group each with 45 cases. The control group was given Mesalazin Enteric-coated Tablets 1 g/times, 4 times/d, continuous treatment for 1 months:when the clinical symptoms were stable, switched to 500 mg/times, 3 times/d, continuous treatment for 3 months. The observation group on the basis of conventional treatment to give Bifidobacterium triple live bacteria scattered adjuvant therapy (Bifico capsule), 0.84 g/time, 2 times/d, continuous treatment for 3 months. In the two groups, the levels of inflammatory factors (IL-6, IL-10, CRP, TNF-α), oxidative stress markers (MDA, SOD) and T lymphocyte subsets (CD3+, CD4+, CD8+, CD4+/CD8+) were compared pre and post treatment. Results: The levels of IL-6, CRP, MDA, TNF-α, CD8+ in both two groups were significantly decreased compared with treatment before;the levels of IL-10, SOD, CD3+, CD4+ and CD4+/CD8+were significantly increased compared with treatment before. After treatment, the levels of serum IL-6, CRP, TNF-α, MDA and CD8+ in observation group with the data (72.17±15.18) pg/mL, (21.52±10.21) mg/mL, (15.98±4.12) pg/mL respectively were decreased more significantly than those data in control group which were (86.55±17.26) pg/mL, (43.02±12.27) mg/L, (22.35±3.67) pg/mL, MDA level (5.89±0.56) nmol/mL in observation group was decreased more significantly than the level in the control group (6.75±0.68) nmol/mL;CD8+level (17.24±3.06)% in observation group was decreased more significantly than the level (19.01±2.62)% in the control group. After treatment, IL-10 level (70.21±6.03) pg/mL in observation group was increased more significantly than the level in the control group (56.48+ 8.67) pg/mL;SOD level (1.84±0.06) U/mL in observation group was increased more significantly than the level in the control group (1.32±0.05) U/mL;the levels of CD3+, CD4+, CD4+/CD8+ in the observation group (57.84±6.07)%;(36.78±4.32)%;(1.92±0.29) were increased more significantly than the level in the control group (54.93±6.87)%;(35.42±5.27)%;(1.89±0.12). Conclusion: Bifidobacterium triple live bacteria scattered adjuvant treatment of ulcerative colitis helps regulate oxidative stress and inflammatory cytokines inhibiting inflammatory reaction and improving enhance function, and can effectively improve the clinical symptoms of patients with UC.

Bioleaching of Zn(Ⅱ) and Pb(Ⅱ) from Nigerian sphalerite and galena ores by mixed culture of acidophilic bacteria

Zn（Ⅱ） and Pb（Ⅱ） from Nigerian sphalerite and galena ores were bioleached by a mixed culture of acidophilic bacteria.The influences of pH and ferric ion on the bioleaching rates of sphalerite and galena were examined.The result shows that pH 2.1 and 2.7 are favourable for the leaching of Zn（Ⅱ） and Pb（Ⅱ） from sphalerite and galena,respectively.It was observed that the use of agarose-simulated media caused cells to excrete exopolymers containing ferric ions which enhanced oxidation.The oxidation equilibrium for sphalerite and galena took 3 and 4 d,respectively.About 38.3% sphalerite and 34.2% galena were leached within 1 d and approximately 92.0% Zn（Ⅱ） and 89.0% Pb（Ⅱ） were recovered in 5 d,respectively.The unleached residual products were examined by X-ray diffraction for sphalerite,revealing the presence of elemental sulphur（S）,zinc sulphate（ZnSO4） and few traces of calcium aluminate（Ca3Al2O6）.The XRD pattern also indicates the presence of elemental sulphur（S）,lead sulphate（PbSO4） and few traces of itoite [Pb（S,Ge）（O,OH）4] and cobalt lead silicate [Pb8Co（Si2O7）3] in the unleached galena ore.

Effect of Zinc Oxide Nanoparticles on Denitrification and Denitrifying Bacteria Communities in Typical Estuarine Sediments

For revealing the effects of increasing of zinc oxide nanoparticles(ZnO NPs)on denitrification and denitrifying bacteria communities in estuarine sediments,the surface sediments of two typical estuaries(the Yangtze River Estuary and the Yellow River Estuary)were added with medium concentration(170mgL−1)and high concentration(1700mgL−1)of ZnO NPs for anaerobic cul-ture in laboratory.The concentration of NO_(3)^(−)and NO_(2)^(−),the reductase activity and denitrification rate were measured by physico-chemical analysis,nirS gene abundance and denitrifying bacteria communities by molecular biological methods.The results showed that ZnO NPs inhibited NO_(3)^(−), NO_(2)^(−)reduction process and NO_(3)^(−), NO_(2)^(−)reductase activity,and a stronger inhibition effect resulting from the higher ZnO NPs concentration.ZnO NPs decreased nirS gene abundance and community diversity of denitrifying bacteria.In addition,the inhibition degree of ZnO NPs on the denitrification process of sediments in different estuaries was different.These results were of great significance for evaluating the potential ecological toxicity and risks of nanomaterials in estuaries.

Contribution of trimethylamine N-oxide on the growth and pressure tolerance of deep-sea bacteria

Trimethylamine N-oxide(TMAO) is widely dispersed in marine environments and plays an important role in the biogeochemical cycle of nitrogen. Diverse marine bacteria utilize TMAO as carbon and nitrogen sources or as electron acceptor in anaerobic respiration. Alteration of respiratory component according to the pressure is a common trait of deep-sea bacteria. Deep-sea bacteria from dif ferent genera harbor high hydrostatic pressure(HHP) inducible TMAO reductases that are assumed to be constitutively expressed in the deep-sea piezosphere and facilitating quick reaction to TMAO released from ?sh which is a potential nutrient for bacterial growth. However, whether deep-sea bacteria universally employ this strategy remains unknown. In this study, 237 bacterial strains affliated to 23 genera of Proteobacteria,Bacteroidetes, Firmicutes and Actinobacteria were isolated from seawater, sediment or amphipods collected at dif ferent depths. The pressure tolerance and the utilization of TMAO were examined in 74 strains. The results demonstrated no apparent correlation between the depth where the bacteria inhabit and their pressure tolerance, regarding to our samples. Several deep-sea strains from the genera of Alteromonas, Halomonas,Marinobacter, Photobacterium, and Vibrio showed capacity of TMAO utilization, but none of the isolated Acinebacter, Bacillus, Brevundimonas, Muricauda, Novosphingobium, Rheinheimera, Sphingobium and Stenotrophomonas did, indicating the utilization of TMAO is a species-speci?c feature. Furthermore, we noticed that the ability of TMAO utilization varied among strains of the same species. TMAO has greater impact on the growth of deep-sea isolates of Vibrio neocaledonicus than shallow-water isolates. Taken together, the results describe for the ?rst time the TMAO utilization in deep-sea bacterial strains, and expand our understanding of the physiological characteristic of marine bacteria.

Comparison of the serum contents of inflammatory mediators and oxidative stress mediators between patients with gram-positive bacteria and gram-negative bacteria infection

Objective: To compare the serum contents of inflammatory mediators and oxidative stress mediators between patients with gram-positive bacteria and gram-negative bacteria infection. Methods: Patients who were diagnosed with bloodstream bacterial infection in Zigong Third People's Hospital between March 2015 and April 2017 were selected as the research subjects and divided into gram-positive group and gram-negative group according to the results of blood culture and strain identification, and serum levels of inflammatory mediators PCT, IL-1β, IL-6, sTREM-1, TNF-α, NGAL, SAA, HPT and hs-CRP as well as oxidative stress mediators MDA, AOPP, TAC, CAT and SOD were determined. Results: Serum PCT, IL-1β, IL-6, sTREM-1, TNF-α, NGAL, SAA, HPT, hs-CRP, MDA and AOPP levels of gram-negative group were greatly higher than those of gram-positive group while TAC, CAT and SOD levels were greatly lower than those of gram-positive group. Conclusion: The changes of inflammatory mediators and oxidative stress mediators in the serum of patients with gram-negative bacteria infection are more significant than those of patients with gram-positive bacteria infection.

Community analysis of ammonia oxidizer in the oxygen-limited nitritation stage of OLAND system by DGGE of PCR amplified 16S rDNA fragments and FISH

OLAND(oxygen limited autotrophic nitrification and denitrification) nitrogen removal system was constructed by coupling with oxygen limited nitritation stage and anaerobic ammonium oxidation stage. Ammonia oxidizer, as a kind of key bacteria in N cycle, plays an important role at the oxygen limited nitritation stage of OLAND nitrogen removal system. In this study, specific amplification of 16S rDNA fragment of ammonia oxidizer by nested PCR, separation of mixed PCR samples by denaturing gradient gel electrophoresis(DGGE), and the quantification of ammonia oxidizer by fluorescence in situ hybridization(FISH) were combined to investigate the shifts of community composition and quantity of ammonia oxidizer of the oxygen limited nitritation stage in OLAND system. It showed that the community composition of ammonia oxidizer changed drastically when dissolved oxygen was decreased gradually, and the dominant ammonia oxidizer of the steady nitrite accumulation stage were completely different from that of the early stage of oxygen limited nitritation identified by DGGE . It was concluded that the Nitrosomonas may be the dominant genus of ammonia oxidizer at the oxygen limited nitritation stage of OLAND system characterized by nested PCR-DGGE and FISH, and the percentage of Nitrosomonas was 72.5% ±0.8% of ammonia oxidizer at the steady nitrite accumulation stage detected by FISH.