Ammonia-oxidizing archaea(AOA),ammonia-oxidizing bacteria(AOB),and anaerobic ammonia-oxidation(anammox)bacteria are very important contributors to nitrogen cycling in natural environments.Functional gene abundances of...Ammonia-oxidizing archaea(AOA),ammonia-oxidizing bacteria(AOB),and anaerobic ammonia-oxidation(anammox)bacteria are very important contributors to nitrogen cycling in natural environments.Functional gene abundances of these microbes were believed to be well relevant to N-cycling in groundwater systems,especially in the Pearl River Delta(PRD)groundwater with unique high intrinsic ammonia concentrations.In this research,20 sediment samples from two in the PRD were collected for porewater chemistry analysis and quantification of N-cycling related genes,including archaeal and bacterial amoA gene and anammox 16S ribosomal Ribonucleic Acid(rRNA)gene.Quantitative Polymerase Chain Reaction(qPCR)results showed that gene abundances of AOA,AOB,and anammox bacteria ranged from 3.13×10^(5)to 3.21×10^(7),1.83×10^(4)to 2.74×10^(6),and 9.27×10^(4)to 8.96×10^(6)copies/g in the sediment of the groundwater system,respectively.Anammox bacteria and AOA dominated in aquitards and aquifers,respectively,meanwhile,the aquitard-aquifer interfaces were demonstrated as ammonium-oxidizing hotspots in the aspect of gene numbers.Gene abundances of nitrifiers were analyzed with geochemistry profiles.Correlations between gene numbers and environmental variables indicated that the gene abundances were impacted by hydrogeological conditions,and microbial-derived ammonium loss was dominated by AOA in the northwest PRD and by anammox bacteria in the southeast PRD.展开更多
利用荧光定量PCR、末端限制性片段长度多样性(T-RFLP)和基因克隆文库技术,比较了4种施氮水平(不施氮肥,0 kg N/hm^2,CK;施低水平氮肥,75 kg N/hm^2,N1;施中水平氮肥,150 kg N/hm^2,N2;施高水平氮肥,225 kg N/hm^2,N3)下华北平原地区小...利用荧光定量PCR、末端限制性片段长度多样性(T-RFLP)和基因克隆文库技术,比较了4种施氮水平(不施氮肥,0 kg N/hm^2,CK;施低水平氮肥,75 kg N/hm^2,N1;施中水平氮肥,150 kg N/hm^2,N2;施高水平氮肥,225 kg N/hm^2,N3)下华北平原地区小麦季表层(0—20 cm)土壤总细菌、氨氧化细菌(AOB)和氨氧化古菌(AOA)的丰度和群落结构。结果表明,土壤总细菌、AOB和AOA数量分别在每克干土5.74×10~9—7.50×10~9、8.89×10~6—2.66×10~7和3.83×10~8—7.78×10~8之间。不同施氮量土壤AOA数量均高于AOB数量,AOA/AOB值在81.72—14.38之间。增施氮肥显著显著提高AOB数量(P<0.05),对总细菌和AOA数量的影响不显著(P>0.05)。与CK相比,处理N1、N2和N3中AOB数量分别提高了0.64、1.50和1.99倍。增施氮肥显著改变了AOB和AOA的群落结构,且不同施氮量处理中AOB群落结构差异更大。系统进化分析显示,施氮肥小麦土壤AOB主要为Nitrosospira属类群,分布在Cluster 3的两个分支中;AOA分布在Cluster S的4个分支中。相关性分析显示,AOB数量与全氮和铵态氮含量呈显著正相关关系,与土壤pH和碳氮比呈显著负相关关系(P<0.05);AOA数量与硝态氮含量和土壤pH呈显著正相关关系,与铵态氮含量呈显著负相关关系(P<0.05)。研究结果表明:增施氮肥可显著改变华北平原地区碱性土壤AOB数量与群落结构,该地区小麦土壤中AOB比AOA对氮肥响应更敏感。展开更多
基金This study was financially supported by the General Research Fund of the Research Grants Council,the Hong Kong Special Administrative Region,China(HKU 702612P and HKU 703010P).
文摘Ammonia-oxidizing archaea(AOA),ammonia-oxidizing bacteria(AOB),and anaerobic ammonia-oxidation(anammox)bacteria are very important contributors to nitrogen cycling in natural environments.Functional gene abundances of these microbes were believed to be well relevant to N-cycling in groundwater systems,especially in the Pearl River Delta(PRD)groundwater with unique high intrinsic ammonia concentrations.In this research,20 sediment samples from two in the PRD were collected for porewater chemistry analysis and quantification of N-cycling related genes,including archaeal and bacterial amoA gene and anammox 16S ribosomal Ribonucleic Acid(rRNA)gene.Quantitative Polymerase Chain Reaction(qPCR)results showed that gene abundances of AOA,AOB,and anammox bacteria ranged from 3.13×10^(5)to 3.21×10^(7),1.83×10^(4)to 2.74×10^(6),and 9.27×10^(4)to 8.96×10^(6)copies/g in the sediment of the groundwater system,respectively.Anammox bacteria and AOA dominated in aquitards and aquifers,respectively,meanwhile,the aquitard-aquifer interfaces were demonstrated as ammonium-oxidizing hotspots in the aspect of gene numbers.Gene abundances of nitrifiers were analyzed with geochemistry profiles.Correlations between gene numbers and environmental variables indicated that the gene abundances were impacted by hydrogeological conditions,and microbial-derived ammonium loss was dominated by AOA in the northwest PRD and by anammox bacteria in the southeast PRD.
文摘利用荧光定量PCR、末端限制性片段长度多样性(T-RFLP)和基因克隆文库技术,比较了4种施氮水平(不施氮肥,0 kg N/hm^2,CK;施低水平氮肥,75 kg N/hm^2,N1;施中水平氮肥,150 kg N/hm^2,N2;施高水平氮肥,225 kg N/hm^2,N3)下华北平原地区小麦季表层(0—20 cm)土壤总细菌、氨氧化细菌(AOB)和氨氧化古菌(AOA)的丰度和群落结构。结果表明,土壤总细菌、AOB和AOA数量分别在每克干土5.74×10~9—7.50×10~9、8.89×10~6—2.66×10~7和3.83×10~8—7.78×10~8之间。不同施氮量土壤AOA数量均高于AOB数量,AOA/AOB值在81.72—14.38之间。增施氮肥显著显著提高AOB数量(P<0.05),对总细菌和AOA数量的影响不显著(P>0.05)。与CK相比,处理N1、N2和N3中AOB数量分别提高了0.64、1.50和1.99倍。增施氮肥显著改变了AOB和AOA的群落结构,且不同施氮量处理中AOB群落结构差异更大。系统进化分析显示,施氮肥小麦土壤AOB主要为Nitrosospira属类群,分布在Cluster 3的两个分支中;AOA分布在Cluster S的4个分支中。相关性分析显示,AOB数量与全氮和铵态氮含量呈显著正相关关系,与土壤pH和碳氮比呈显著负相关关系(P<0.05);AOA数量与硝态氮含量和土壤pH呈显著正相关关系,与铵态氮含量呈显著负相关关系(P<0.05)。研究结果表明:增施氮肥可显著改变华北平原地区碱性土壤AOB数量与群落结构,该地区小麦土壤中AOB比AOA对氮肥响应更敏感。
