Ammonia induces astrocyte swelling, which is strongly associated with overexpression of aquaporin-4. However, the mechanisms by which ammonia induces astrocyte swelling, and subsequently upregulating aquaporin-4 expre...Ammonia induces astrocyte swelling, which is strongly associated with overexpression of aquaporin-4. However, the mechanisms by which ammonia induces astrocyte swelling, and subsequently upregulating aquaporin-4 expression, remain unknown. In the present study, astrocytes were cultured in vitro and exposed to ammonium chloride (NH4CI), followed by propofol protein kinase C agonist, or antagonist, respectively. Astrocyte morphology was observed by light microscopy, and aquaporin-4 expression was detected by western blot analysis. Results showed that propofol or protein kinase C agonist significantly attenuated the degree of NH4CI-induced astrocyte swelling and inhibited increased aquaporin-4 expression. Propofol treatment inhibited aquaporin-4 overexpression in cultured astrocyte induced by NH4CI; protein kinase C pathway activation is potentially involved.展开更多
The leaching kinetics of Tang-dan refractory low grade complex copper ore was investigated in ammonia-ammonium chloride solution.The concentration of ammonia and ammonium chloride,the ore particle size,the solid-to-li...The leaching kinetics of Tang-dan refractory low grade complex copper ore was investigated in ammonia-ammonium chloride solution.The concentration of ammonia and ammonium chloride,the ore particle size,the solid-to-liquid ratio and the temperature were chosen as parameters in the experiments.The results show that temperature,concentration of ammonia and ammonium chloride have favorable influence on the leaching rate of copper oxide ores.But,leaching rate decreases with increasing particle size and solid-to-liquid ratio.The leaching process is controlled by the diffusion of the lixiviant and the activation energy is determined to be 23.279 kJ/mol.An equation was also proposed to describe the leaching kinetics.展开更多
In order to decrease the evaporating rate of ammonia and increase the solubility of copper in the solution,ethylenediamine was added into the ammonia-ammonium chloride system to leach the copper-containing oxide ores....In order to decrease the evaporating rate of ammonia and increase the solubility of copper in the solution,ethylenediamine was added into the ammonia-ammonium chloride system to leach the copper-containing oxide ores.The thermodynamic model was constructed and the solubility of malachite Cu2(OH)2CO3 in the ammonia-ammonium chloride-ethylenediamine(En)-water system was calculated using the exponential computation method based on both mass balance and charge balance.It is found that the solubility of copper can be increased and the free ammonia concentration can be decreased by submitting partial ammonia with ethylenediamine.The lower free ammonia concentration in the solution is a guarantee to the lower evaporating rate of ammonia.The conditions of malachite Cu2(OH)2CO3 converting to atacamite Cu(OH)1.5Cl0.5 were also studied.A group of experiments were designed to validate the veracity of the results of the thermodynamic calculation.It is found that the thermodynamic model is reliable and it can guide the leaching process.展开更多
The flocculation behavior of ultrafine kaolinite suspension was investigated through settlement tests and FTIR method was employed to probe the adsorption mechanism of flocculant on kaolinite. The results show that th...The flocculation behavior of ultrafine kaolinite suspension was investigated through settlement tests and FTIR method was employed to probe the adsorption mechanism of flocculant on kaolinite. The results show that the maximum settling rate of kaolinite occurs at pH value of 3.33,which is close to the point of zero charge(PZC) of kaolinite (3.5). This result is in good agreement with the double electric layer theory. Kaolinite suspension reaches the largest settling rate at a low concentration of 39 g/t for poly diallyl-dimethyl-ammonium chloride(PDADMA) flocculant,whereas for polyacrylamides(PAM) the dosage is required to be 500 g/t. When macromolecule polymer is adsorbed on surface,kaolinite particles may be flocculant due to the bridging effect. There are cation flocculant characteristic bands on the spectrum of kaolinite but no obvious shifting. Thus,the adsorption of poly diallyl-dimethyl-ammonium chloride on kaolinite surface is physical adsorption.展开更多
Tertiary amine was synthesized from fatty amine and formaldehyde. And then the synthesized tertiary amine was used to react with benzyl chloride to synthesize hexadecyl dimethyl benzyl ammonium chloride (1627) at ambi...Tertiary amine was synthesized from fatty amine and formaldehyde. And then the synthesized tertiary amine was used to react with benzyl chloride to synthesize hexadecyl dimethyl benzyl ammonium chloride (1627) at ambient pressure. Using the synthesized 1627 as collector,the flotation properties of diaspore and kaolinite were investigated by single mineral and mixed mineral test. The flotation mechanism of diaspore,kaolinite and 1627 was discussed based on FTIR spectra. The results show that the mass ratio of aluminum to silicate achieves 15.02 and the recovery of alumina in concentrate is 43.07% using 1627 as a collector. The 1627 is found to be a more effective and a promising collector for reverse flotation to remove aluminum-silicate minerals from bauxite.展开更多
Taking the saline lake bischofite and NH4Cl that was removed with the ammonia method and continuously followed by filtration as raw materials with a molar ratio of 1∶1 of MgCl2 to NH4Cl, ammonium carnallite was synth...Taking the saline lake bischofite and NH4Cl that was removed with the ammonia method and continuously followed by filtration as raw materials with a molar ratio of 1∶1 of MgCl2 to NH4Cl, ammonium carnallite was synthesized. And then the ammonium carnallite was dehydrated to some extent at 160℃ for 4 h. Ammonium carnallite reacted with ammonia at 240℃ for 150 min and the ammonation ammonium carnallite was produced. Finally, the ammonation ammonium carnallite was calcined at 750℃ into anhydrous magnesium chloride containing only 0.1%(mass fraction) of MgO. On the other hand, dehydrated ammonium carnallite was mixed with the solid ammonium chloride at mass ratio 1∶4 at high temperature and with the differential pressure of NH3 above 30.5kPa. The dehydrated ammonium carnallite of mixture was dehydrated at 410℃, and then calcined at 700℃ into anhydrous magnesium chloride with only 0.087%(mass fraction) of MgO. X-ray diffraction and electron microscopy analysis results prove that anhydrous magnesium chloride obtained by both methods hasn’t mixed phases, the particle is large and even has good dispersion, which is suitable for preparation of metal magnesium in the electrolysis.展开更多
In order to provide references for selecting highly efficient red mud flocculants, the behaviour of polyacryl- amidomethyltrimethyl ammonium chloride (PATAC) in red mud separation process was investigated. PATAC was e...In order to provide references for selecting highly efficient red mud flocculants, the behaviour of polyacryl- amidomethyltrimethyl ammonium chloride (PATAC) in red mud separation process was investigated. PATAC was employed as a flocculant for red mud separation from the caustic aluminate liquor at 95 ℃. The used red mud was generated from Chinese diaspore bauxite in Bayer process of alumina production. And the changes of PATAC before or after being treated in caustic solution at 95 ℃ were studied by thermogravimetry (TG) and Fourier transform infrared (FTIR) spectral analysis. The results show that PATAC fails in effectively flocculating red mud and PATAC is readily converted to a quaternary ammonium hydroxide (PATAH) in caustic solution. PATAH can be decomposed to a new polymer (HPATAH) even at 95 ℃. Furthermore, there is an intramolecular hydrogen bond formed in the HPATAH polymer chain with two functional groups of —CH2—OH and —CONH2. Therefore, the poor flocculation property of PATAC for red mud separation can be attributed to the thermal decomposition of PATAC in the caustic red mud slurry at 95 ℃ and the formation of intramolecular hydrogen bond in the polymer chain of HPATAH during the thermal decomposition, which causes the absorbable functional groups of PATAC to decrease greatly.展开更多
The dissolution kinetics of calcined ulexite in ammonium chloride solutions at high solid-to-liquid ratios were investigated. In the experiments, calcination temperature, solution concentration, reaction temperature, ...The dissolution kinetics of calcined ulexite in ammonium chloride solutions at high solid-to-liquid ratios were investigated. In the experiments, calcination temperature, solution concentration, reaction temperature, and pre-hydration were chosen as parameters. It was observed that the dissolution rate increased with increasing calcination temperature, solution concentration, and reaction temperature, whereas it was not affected by pre-hydration. Employing graphical and statistic methods, the dissolution rate, based on homogeneous reaction model, can be given as: (1-X(B2O3))-1-1= k(c(NH4Cl))1.982t. The activation energy for the dissolution of the ulexite sample calcined at 160 ℃ was found to be 84.04 kJ·mol-1.展开更多
Starch is easy to breed bacteria,which affects its performance and application in medical dressings.In this research,antibacterial starch was prepared by physical blending method with glycidyl trimethyl ammonium chlor...Starch is easy to breed bacteria,which affects its performance and application in medical dressings.In this research,antibacterial starch was prepared by physical blending method with glycidyl trimethyl ammonium chloride(GTA)and potassium sorbate as antibacterial agents.The antibacterial activities of GTA,potassium sorbate,GTA-starch and potassium sorbate-starch were investigated with the purpose of understanding the growth of bacteria in starch system.Results showed that GTA had good antibacterial activity,and the bacteriostasis rate of 0.05 g/mL GTA against Staphylococcus aureus(S.aureus)was 96.55%.The antibacterial properties of starch increased with an increase of mass fraction of potassium sorbate and then decreased with the continuously increasing of mass fraction of GTA.GTA-starch with30%GTA showed the best antibacterial activities,the bacteriostasis rate of Escherichia coli(E.coli)and S.aureus were 26.48%and20.26%,respectively;50%potassium sorbate in potassium sorbatestarch showed the best antibacterial activities,the bacteriostasis rate of E.coli and S.aureus were 67.68%and 74.75%,respectively.展开更多
Reaction of imines with 2-silyloxydiene catalyzed by ammonium chloride has been perfectly proceeded under environmentally friendly conditions to give Mannich-type product selectively. The reaction would proceed via Ma...Reaction of imines with 2-silyloxydiene catalyzed by ammonium chloride has been perfectly proceeded under environmentally friendly conditions to give Mannich-type product selectively. The reaction would proceed via Mannich-type mechanism, not cyclization/ring-opening process. Cyclopropanation of the corresponding Mannich-type product gave the precursor of prasugrel skeleton in high yield.展开更多
We previously demonstrated that 2-hydroxypropyltrimethyl ammonium chloride chitosan(HACC)promoted the production of nitric oxide(NO)and proinflammatory cytokines by activating the mitogen-activated protein kinases(MAP...We previously demonstrated that 2-hydroxypropyltrimethyl ammonium chloride chitosan(HACC)promoted the production of nitric oxide(NO)and proinflammatory cytokines by activating the mitogen-activated protein kinases(MAPK)and Janus kinase(JAK)/STAT pathways in RAW 264.7 cells,indicating good immunomodulatory activity of HACC.In this study,to further investigate the immunomodulatory mechanisms of HACC,we determined the roles of phosphatidylinositol 3-kinase(PI3K)/Akt,activating protein(AP-1)and nuclear factor kappa B(NF-κB)in HACC-induced activation of RAW 264.7 cells by the western blotting.The results suggest that HACC promoted the phosphorylation of p85 and Akt.Furthermore,c-Jun and p65 were also increased after the treatment of RAW 264.7 cells with HACC,indicating the translocation of NF-κB and AP-1 from cytoplasm to nucleus.In addition,as scanning electron microscopy(SEM)analysis shows,the cell morphology changed after HACC treatment.These findings indicate that HACC activated MAPK,JAK/STAT,and PI3K/Akt signaling pathways dependent on AP-1 and NF-κB activation in RAW 264.7 cells,ultimately leading to the increase of NO and cytokines.展开更多
Bacteriostatic laundry detergent was prepared with application of dodecyl dimethyl benzyl ammonium chloride (1227). In accordance with relevant standards applied in Chinese detergent industry, the effect of 1227 on se...Bacteriostatic laundry detergent was prepared with application of dodecyl dimethyl benzyl ammonium chloride (1227). In accordance with relevant standards applied in Chinese detergent industry, the effect of 1227 on sensory and physiochemical quality indices, detergency and foaming performance of laundry detergent were comprehensively investigated,and antimicrobial performance as well as practical application performance including skin irritation of laundry detergent formulated with 1227 was emphatically examined and compared with that of market product with similar functions. Results indicated that, in formulation without anionic surfactant, all indices of self-prepared bacteriostatic laundry detergent can meet requirements specified in relevant standards. The foaming power of self-prepared bacteriostatic laundry detergent was lower and easier to rinse than bacteriostatic laundry detergent product in market. Using aqueous solution of 1% mass fraction of self-prepared bacteriostatic laundry detergent containing 2%of 1227, the antibacterial rate for 20 min on both Escherichia coli and Staphylococcus aureus separately reached 100%, which was much better than that of the bacteriostatic laundry detergent product from market. The action effectiveness of the self-prepared bacteriostatic laundry detergent can be maintained for a longer time period and shows no obvious irritation effect to human skin.展开更多
Alzheimer disease is one of the commonest neurological diseases which is characterized by amyloid plaques accumulation in multiple brain regions. This study investigated the potential neuroprotective effect of artesun...Alzheimer disease is one of the commonest neurological diseases which is characterized by amyloid plaques accumulation in multiple brain regions. This study investigated the potential neuroprotective effect of artesunate on aluminum induced neurotoxicity vs memantine in rats. 40 male albino Wistar rats were divided randomly into 4 groups as follow: Group 1 negative control, group 2 positive control group induced by ammonium chloride, group 3 rats treated by NH4Cl + artesunate solution, group 4 rats treated by NH4Cl + memantine S.C. spatial Memory and Learning were evaluated using Morris Water Maze (MWM) test. Malondialdehyde (MDA) and reduced glutathione (GSH) levels were measured in cerebral cortex tissue homogenate. Tumor necrosis factor-α (TNFα) and interleukin-1 beta (IL-1β) concentrations were measured in rat cerebral cortex tissue homogenate using rat enzyme linked immunosorbent assay (ELISA) kits. Real-time quantitative reverse transcription-polymerase chain reaction (Real-time qRT-PCR) for Caspase-3, Bcl-2 and iNOS gene expression was measured in rat cerebral cortex. Slices from cerebral cortex were studied by histopathological examination. Artesunate significantly decreased MDA level and inhibited iNOS, caspase and upregulated Bcl-2 gene expression in cerebral cortex. ART increased significantly antioxidant level GSH, and decreased significantly TNF-alpha and IL-B levels. It reduced significantly 1ry retention latency, 2ry retention latency and initial acquisition latency. It also improved brain histopathology and decreased amyloid plaque deposition. ART exerted neuroprotective effect through oxidative stress correction and enhancement of antiapoptotic markers in neuronal cells of the cerebral cortex.展开更多
基金Supported by a Grant from Zhejiang Provincial Health Department, No. 2007A057
文摘Ammonia induces astrocyte swelling, which is strongly associated with overexpression of aquaporin-4. However, the mechanisms by which ammonia induces astrocyte swelling, and subsequently upregulating aquaporin-4 expression, remain unknown. In the present study, astrocytes were cultured in vitro and exposed to ammonium chloride (NH4CI), followed by propofol protein kinase C agonist, or antagonist, respectively. Astrocyte morphology was observed by light microscopy, and aquaporin-4 expression was detected by western blot analysis. Results showed that propofol or protein kinase C agonist significantly attenuated the degree of NH4CI-induced astrocyte swelling and inhibited increased aquaporin-4 expression. Propofol treatment inhibited aquaporin-4 overexpression in cultured astrocyte induced by NH4CI; protein kinase C pathway activation is potentially involved.
基金Projects(2007CB613604) supported by the National Basic Research Program of ChinaProject(50674104) supported by the National Natural Science Foundation of China
文摘The leaching kinetics of Tang-dan refractory low grade complex copper ore was investigated in ammonia-ammonium chloride solution.The concentration of ammonia and ammonium chloride,the ore particle size,the solid-to-liquid ratio and the temperature were chosen as parameters in the experiments.The results show that temperature,concentration of ammonia and ammonium chloride have favorable influence on the leaching rate of copper oxide ores.But,leaching rate decreases with increasing particle size and solid-to-liquid ratio.The leaching process is controlled by the diffusion of the lixiviant and the activation energy is determined to be 23.279 kJ/mol.An equation was also proposed to describe the leaching kinetics.
基金Project(2007CB613604)supported by the National Basic Research Program of ChinaProject(50674104)supported by the National Natural Science Foundation of China
文摘In order to decrease the evaporating rate of ammonia and increase the solubility of copper in the solution,ethylenediamine was added into the ammonia-ammonium chloride system to leach the copper-containing oxide ores.The thermodynamic model was constructed and the solubility of malachite Cu2(OH)2CO3 in the ammonia-ammonium chloride-ethylenediamine(En)-water system was calculated using the exponential computation method based on both mass balance and charge balance.It is found that the solubility of copper can be increased and the free ammonia concentration can be decreased by submitting partial ammonia with ethylenediamine.The lower free ammonia concentration in the solution is a guarantee to the lower evaporating rate of ammonia.The conditions of malachite Cu2(OH)2CO3 converting to atacamite Cu(OH)1.5Cl0.5 were also studied.A group of experiments were designed to validate the veracity of the results of the thermodynamic calculation.It is found that the thermodynamic model is reliable and it can guide the leaching process.
基金Project(2005CB623701) supported by the Major State Basic Research Development Program of China
文摘The flocculation behavior of ultrafine kaolinite suspension was investigated through settlement tests and FTIR method was employed to probe the adsorption mechanism of flocculant on kaolinite. The results show that the maximum settling rate of kaolinite occurs at pH value of 3.33,which is close to the point of zero charge(PZC) of kaolinite (3.5). This result is in good agreement with the double electric layer theory. Kaolinite suspension reaches the largest settling rate at a low concentration of 39 g/t for poly diallyl-dimethyl-ammonium chloride(PDADMA) flocculant,whereas for polyacrylamides(PAM) the dosage is required to be 500 g/t. When macromolecule polymer is adsorbed on surface,kaolinite particles may be flocculant due to the bridging effect. There are cation flocculant characteristic bands on the spectrum of kaolinite but no obvious shifting. Thus,the adsorption of poly diallyl-dimethyl-ammonium chloride on kaolinite surface is physical adsorption.
基金Project(2006AA06Z120) supported by the National High-Tech Research and Development Program of ChinaProject(2005CB623701) supported by the Major State Basic Research Development Program of China
文摘Tertiary amine was synthesized from fatty amine and formaldehyde. And then the synthesized tertiary amine was used to react with benzyl chloride to synthesize hexadecyl dimethyl benzyl ammonium chloride (1627) at ambient pressure. Using the synthesized 1627 as collector,the flotation properties of diaspore and kaolinite were investigated by single mineral and mixed mineral test. The flotation mechanism of diaspore,kaolinite and 1627 was discussed based on FTIR spectra. The results show that the mass ratio of aluminum to silicate achieves 15.02 and the recovery of alumina in concentrate is 43.07% using 1627 as a collector. The 1627 is found to be a more effective and a promising collector for reverse flotation to remove aluminum-silicate minerals from bauxite.
基金Project(2000 G 101) supported by the Key Science and Technology Research Project of Qinghai Province
文摘Taking the saline lake bischofite and NH4Cl that was removed with the ammonia method and continuously followed by filtration as raw materials with a molar ratio of 1∶1 of MgCl2 to NH4Cl, ammonium carnallite was synthesized. And then the ammonium carnallite was dehydrated to some extent at 160℃ for 4 h. Ammonium carnallite reacted with ammonia at 240℃ for 150 min and the ammonation ammonium carnallite was produced. Finally, the ammonation ammonium carnallite was calcined at 750℃ into anhydrous magnesium chloride containing only 0.1%(mass fraction) of MgO. On the other hand, dehydrated ammonium carnallite was mixed with the solid ammonium chloride at mass ratio 1∶4 at high temperature and with the differential pressure of NH3 above 30.5kPa. The dehydrated ammonium carnallite of mixture was dehydrated at 410℃, and then calcined at 700℃ into anhydrous magnesium chloride with only 0.087%(mass fraction) of MgO. X-ray diffraction and electron microscopy analysis results prove that anhydrous magnesium chloride obtained by both methods hasn’t mixed phases, the particle is large and even has good dispersion, which is suitable for preparation of metal magnesium in the electrolysis.
基金Project(2005CB623702) supported by the Major State Basic Research and Development Program of China
文摘In order to provide references for selecting highly efficient red mud flocculants, the behaviour of polyacryl- amidomethyltrimethyl ammonium chloride (PATAC) in red mud separation process was investigated. PATAC was employed as a flocculant for red mud separation from the caustic aluminate liquor at 95 ℃. The used red mud was generated from Chinese diaspore bauxite in Bayer process of alumina production. And the changes of PATAC before or after being treated in caustic solution at 95 ℃ were studied by thermogravimetry (TG) and Fourier transform infrared (FTIR) spectral analysis. The results show that PATAC fails in effectively flocculating red mud and PATAC is readily converted to a quaternary ammonium hydroxide (PATAH) in caustic solution. PATAH can be decomposed to a new polymer (HPATAH) even at 95 ℃. Furthermore, there is an intramolecular hydrogen bond formed in the HPATAH polymer chain with two functional groups of —CH2—OH and —CONH2. Therefore, the poor flocculation property of PATAC for red mud separation can be attributed to the thermal decomposition of PATAC in the caustic red mud slurry at 95 ℃ and the formation of intramolecular hydrogen bond in the polymer chain of HPATAH during the thermal decomposition, which causes the absorbable functional groups of PATAC to decrease greatly.
文摘The dissolution kinetics of calcined ulexite in ammonium chloride solutions at high solid-to-liquid ratios were investigated. In the experiments, calcination temperature, solution concentration, reaction temperature, and pre-hydration were chosen as parameters. It was observed that the dissolution rate increased with increasing calcination temperature, solution concentration, and reaction temperature, whereas it was not affected by pre-hydration. Employing graphical and statistic methods, the dissolution rate, based on homogeneous reaction model, can be given as: (1-X(B2O3))-1-1= k(c(NH4Cl))1.982t. The activation energy for the dissolution of the ulexite sample calcined at 160 ℃ was found to be 84.04 kJ·mol-1.
文摘Starch is easy to breed bacteria,which affects its performance and application in medical dressings.In this research,antibacterial starch was prepared by physical blending method with glycidyl trimethyl ammonium chloride(GTA)and potassium sorbate as antibacterial agents.The antibacterial activities of GTA,potassium sorbate,GTA-starch and potassium sorbate-starch were investigated with the purpose of understanding the growth of bacteria in starch system.Results showed that GTA had good antibacterial activity,and the bacteriostasis rate of 0.05 g/mL GTA against Staphylococcus aureus(S.aureus)was 96.55%.The antibacterial properties of starch increased with an increase of mass fraction of potassium sorbate and then decreased with the continuously increasing of mass fraction of GTA.GTA-starch with30%GTA showed the best antibacterial activities,the bacteriostasis rate of Escherichia coli(E.coli)and S.aureus were 26.48%and20.26%,respectively;50%potassium sorbate in potassium sorbatestarch showed the best antibacterial activities,the bacteriostasis rate of E.coli and S.aureus were 67.68%and 74.75%,respectively.
文摘Reaction of imines with 2-silyloxydiene catalyzed by ammonium chloride has been perfectly proceeded under environmentally friendly conditions to give Mannich-type product selectively. The reaction would proceed via Mannich-type mechanism, not cyclization/ring-opening process. Cyclopropanation of the corresponding Mannich-type product gave the precursor of prasugrel skeleton in high yield.
基金Supported by the National Key R&D Program of China(No.2018YFC0311305)the Key Research and Development Program of Shandong Province(Nos.2019GHY112015,2019YYSP028)。
文摘We previously demonstrated that 2-hydroxypropyltrimethyl ammonium chloride chitosan(HACC)promoted the production of nitric oxide(NO)and proinflammatory cytokines by activating the mitogen-activated protein kinases(MAPK)and Janus kinase(JAK)/STAT pathways in RAW 264.7 cells,indicating good immunomodulatory activity of HACC.In this study,to further investigate the immunomodulatory mechanisms of HACC,we determined the roles of phosphatidylinositol 3-kinase(PI3K)/Akt,activating protein(AP-1)and nuclear factor kappa B(NF-κB)in HACC-induced activation of RAW 264.7 cells by the western blotting.The results suggest that HACC promoted the phosphorylation of p85 and Akt.Furthermore,c-Jun and p65 were also increased after the treatment of RAW 264.7 cells with HACC,indicating the translocation of NF-κB and AP-1 from cytoplasm to nucleus.In addition,as scanning electron microscopy(SEM)analysis shows,the cell morphology changed after HACC treatment.These findings indicate that HACC activated MAPK,JAK/STAT,and PI3K/Akt signaling pathways dependent on AP-1 and NF-κB activation in RAW 264.7 cells,ultimately leading to the increase of NO and cytokines.
文摘Bacteriostatic laundry detergent was prepared with application of dodecyl dimethyl benzyl ammonium chloride (1227). In accordance with relevant standards applied in Chinese detergent industry, the effect of 1227 on sensory and physiochemical quality indices, detergency and foaming performance of laundry detergent were comprehensively investigated,and antimicrobial performance as well as practical application performance including skin irritation of laundry detergent formulated with 1227 was emphatically examined and compared with that of market product with similar functions. Results indicated that, in formulation without anionic surfactant, all indices of self-prepared bacteriostatic laundry detergent can meet requirements specified in relevant standards. The foaming power of self-prepared bacteriostatic laundry detergent was lower and easier to rinse than bacteriostatic laundry detergent product in market. Using aqueous solution of 1% mass fraction of self-prepared bacteriostatic laundry detergent containing 2%of 1227, the antibacterial rate for 20 min on both Escherichia coli and Staphylococcus aureus separately reached 100%, which was much better than that of the bacteriostatic laundry detergent product from market. The action effectiveness of the self-prepared bacteriostatic laundry detergent can be maintained for a longer time period and shows no obvious irritation effect to human skin.
文摘Alzheimer disease is one of the commonest neurological diseases which is characterized by amyloid plaques accumulation in multiple brain regions. This study investigated the potential neuroprotective effect of artesunate on aluminum induced neurotoxicity vs memantine in rats. 40 male albino Wistar rats were divided randomly into 4 groups as follow: Group 1 negative control, group 2 positive control group induced by ammonium chloride, group 3 rats treated by NH4Cl + artesunate solution, group 4 rats treated by NH4Cl + memantine S.C. spatial Memory and Learning were evaluated using Morris Water Maze (MWM) test. Malondialdehyde (MDA) and reduced glutathione (GSH) levels were measured in cerebral cortex tissue homogenate. Tumor necrosis factor-α (TNFα) and interleukin-1 beta (IL-1β) concentrations were measured in rat cerebral cortex tissue homogenate using rat enzyme linked immunosorbent assay (ELISA) kits. Real-time quantitative reverse transcription-polymerase chain reaction (Real-time qRT-PCR) for Caspase-3, Bcl-2 and iNOS gene expression was measured in rat cerebral cortex. Slices from cerebral cortex were studied by histopathological examination. Artesunate significantly decreased MDA level and inhibited iNOS, caspase and upregulated Bcl-2 gene expression in cerebral cortex. ART increased significantly antioxidant level GSH, and decreased significantly TNF-alpha and IL-B levels. It reduced significantly 1ry retention latency, 2ry retention latency and initial acquisition latency. It also improved brain histopathology and decreased amyloid plaque deposition. ART exerted neuroprotective effect through oxidative stress correction and enhancement of antiapoptotic markers in neuronal cells of the cerebral cortex.