In silico prospective analysis of the medicinal plants activity on the CagA oncoprotein from Helicobacter pylori

BACKGROUND Colonization with Helicobacter pylori(H.pylori)has a strong correlation with gastric cancer,and the virulence factor CagA is implicated in carcinogenesis.Studies have been conducted using medicinal plants with the aim of eliminating the pathogen;however,the possibility of blocking H.pylori-induced cell differentiation to prevent the onset and/or progression of tumors has not been addressed.This type of study is expensive and time-consuming,requiring in vitro and/or in vivo tests,which can be solved using bioinformatics.Therefore,prospective computational analyses were conducted to assess the feasibility of interaction between phenolic compounds from medicinal plants and the CagA oncoprotein.AIM To perform a computational prospecting of the interactions between phenolic compounds from medicinal plants and the CagA oncoprotein of H.pylori.METHODS In this in silico study,the structures of the phenolic compounds(ligands)kaempferol,myricetin,quercetin,ponciretin(flavonoids),and chlorogenic acid(phenolic acid)were selected from the PubChem database.These phenolic compounds were chosen based on previous studies that suggested medicinal plants as non-drug treatments to eliminate H.pylori infection.The three-dimensional structure model of the CagA oncoprotein of H.pylori(receptor)was obtained through molecular modeling using computational tools from the I-Tasser platform,employing the threading methodology.The primary sequence of CagA was sourced from GenBank(BAK52797.1).A screening was conducted to identify binding sites in the structure of the CagA oncoprotein that could potentially interact with the ligands,utilizing the GRaSP online platform.Both the ligands and receptor were prepared for molecular docking using AutoDock Tools 4(ADT)software,and the simulations were carried out using a combination of ADT and AutoDock Vina v.1.2.0 software.Two sets of simulations were performed:One involving the central region of CagA with phenolic compounds,and another involving the carboxy-terminus region of CagA with phenolic compounds.The receptor-ligand complexes were then analyzed using PyMol and BIOVIA Discovery Studio software.RESULTS The structure model obtained for the CagA oncoprotein exhibited high quality(C-score=0.09)and was validated using parameters from the MolProbity platform.The GRaSP online platform identified 24 residues(phenylalanine and leucine)as potential binding sites on the CagA oncoprotein.Molecular docking simulations were conducted with the three-dimensional model of the CagA oncoprotein.No complexes were observed in the simulations between the carboxy-terminus region of CagA and the phenolic compounds;however,all phenolic compounds interacted with the central region of the oncoprotein.Phenolic compounds and CagA exhibited significant affinity energy(-7.9 to-9.1 kcal/mol):CagA/kaempferol formed 28 chemical bonds,CagA/myricetin formed 18 chemical bonds,CagA/quercetin formed 16 chemical bonds,CagA/ponciretin formed 13 chemical bonds,and CagA/chlorogenic acid formed 17 chemical bonds.Although none of the phenolic compounds directly bound to the amino acid residues of the K-Xn-R-X-R membrane binding motif,all of them bound to residues,mostly positively or negatively charged,located near this region.CONCLUSION In silico,the tested phenolic compounds formed stable complexes with CagA.Therefore,they could be tested in vitro and/or in vivo to validate the findings,and to assess interference in CagA/cellular target interactions and in the oncogenic differentiation of gastric cells.

Chemical Analysis of Water and the Resource of Br,K Prospects of Oilfield Brines from Ordovician and Carboniferous in Tarim,China

China is lack of bromine and potassium seriously.Oilfield brines is the headline goal of bromine and potassium resources exploration.Applicants grab 24oilfield brines samples from various wells of Ordovician

Manganese potential mapping in western Guangxi-southeastern Yunnan(China) via spatial analysis and modal-adaptive prospectivity modeling

While the region of western Guangxi-southeastern Yunan, China, is known and considered prospective for manganese deposits, carrying out prospectivity mapping in this region is challenging due to the diversity of geological factors, the complexity of geological process and the asymmetry of geo-information. In this work, the manganese potential mapping for further exploration targeting is implemented via spatial analysis and modal-adaptive prospectivity modeling. On the basis of targeting criteria developed by the mineral system approach, the spatial analysis is leveraged to extract the predictor variables to identify features of the geological process. Specifically, a metallogenic field analysis approach is proposed to extract metallogenic information that quantifies the regional impacts of the synsedimentary faults and sedimentary basins. In the integration of the extracted predictor variables, a modal-adaptive prospectivity model is built, which allows to adapt different data availability and geological process. The resulting prospective areas of high potential not only correspond to the areas of known manganese deposits but also provide a number of favorable targets in the region for future mineral exploration.

The Research Progress and Prospect Analysis of Germ-Remaining Rice Whitener

In this paper, the backdrop and significance of the research of germ-remaining rice whitener firstly were expatiated. Then, the study progress in China and overseas was summarized. Lastly, the research prospect of this kind of whitener in China was analyzed. It is hoped that it can coach the design of germ-remaining rice whitener.

Development Situation and Prospect Analysis of Biodegradable Film Industry

Application and promotion of biodegradable plastic film in modern agricultural production construction have become trend and direction of agricultural development.As important production material,biodegradable plastic film not only effectively changes agricultural production mode but also effectively decreases environmental pollution.Accompanied by degradation of new material,upgrading of optimizing process,and infiltration and blending of science and technology in new plastics industry,new biodegradable plastic films have been developed and popularized effectively and rapidly.In this paper,starting from development situation and prospect of degradable plastic film in China,natural-based biodegradable plastics,petroleum-based biodegradable plastics and biological-based biodegradable plastics are introduced in detail from basic material of plastics.The advantages and disadvantages of additive biodegradable film and fully biodegradable film are scientifically analyzed,and seasonal and periodic characteristics of biodegradable plastic film industry are elaborated.Moreover,disadvantages of biodegradable plastic film industry development are explored from technical barrier,brand and channel barriers,and talent barrier.Finally,development trend of biodegradable plastic film industry in China is predicted and analyzed scientifically.The research could provide guidance for the development of biodegradable plastic film industry in China ,research of biodegradable plastic film technology,and demonstration and extension of biodegradable plastic film application.

Quantitative evaluation of hepatitis B virus mutations and hepatocellular carcinoma risk:a meta-analysis of prospective studies

Background： The temporal relationship between hepatitis B virus （HBV） mutations and hepatocellular carcinoma （HCC） remains unclear. Methods： We conducted a meta-analysis including cohort and nested case-control studies to prospectively examine the HCC risk associated with common variants of HBV in the PreS, Enhancer Ⅱ, basal core promoter （BCP） and precore regions. Pertinent studies were identified by searching PubMed, Web of Science and the Chinese Biological Medicine databases through to November 2014. Study-specific risk estimates were combined using fixed or random effects models depending on whether significant heterogeneity was detected. Results： Twenty prospective studies were identified, which included 8 cohort and 12 nested case-control studies. There was an increased risk of HCC associated with any PreS mutations with a pooled relative risk （RR） of 3.82 [95% confidence interval （CI）： 2.59-5.61]. The pooled-RR for PreS deletion was 3.98 （95% CI： 2.28-6.95）, which was higher than that of PreS2 start codon mutation （pooled-RR=2.63, 95% CI： 1.30-5.34）. C1653T in Enhancer Ⅱ was significantly associated with HCC risk （pooled-RR=l.83; 95% CI： 1.21-2.76）. For mutations in BCP, statistically significant pooled-RRs of HCC were obtained for T1753V （pooled- RR=2.09; 95% CI： 1.49-2.94） and AI762T/G1764A double mutations （pooled-RR=3.11; 95% CI： 2.08- 4.64）. No statistically significant association with HCC risk was observed for G1896A in the precore region （pooled-RR=0.77; 95% CI： 0.47-1.26）. Conclusions： This study demonstrated that PreS mutations, C1653T, T1753V, and A1762T/G1764A, were associated with an increased risk of HCC. Clinical practices concerning the HCC risk prediction and diagnosis may wish to focus on patients with these mutations.

Life cycle assessment as a prospective tool for sustainable agriculture and food planning at a local level

Owing to the far-reaching environmental consequences of agriculture and food systems,such as their contribution to climate change,there is an urgent need to reduce their impact.International and national governments set sustainability targets and implement corresponding measures.Nevertheless,critics of the globalized system claim that a territorial administrative scale is better suited to address sustainability issues.Yet,at the subnational level,local authorities rarely apply a systemic environmental assessment to enhance their action plans.This paper employs a territorial life cycle assessment methodology to improve local environmental agri-food planning.The objective is to identify significant direct and indirect environmental hotspots,their origins,and formulate effective mitigation strategies.The methodology is applied to the administrative department of Finistere,a strategic agricultural region in North-Western France.Multiple environmental criteria including climate change,fossil resource scarcity,toxicity,and land use are modeled.The findings reveal that the primary environmental hotspots of the studied local food system arise from indirect sources,such as livestock feed or diesel consumption.Livestock reduction and organic farming conversion emerge as the most environmentally efficient strategies,resulting in a 25%decrease in the climate change indicator.However,the overall modeled impact reduction is insufficient following national objectives and remains limited for the land use indicator.These results highlight the innovative application of life cycle assessment led at a local level,offering insights for the further advancement of systematic and prospective local agri-food assessment.Additionally,they provide guidance for local authorities to enhance the sustainability of planning strategies.

Analysis on Prospecting Potential of Tungsten Polymetallic Ore in Haergeng of Gonghe County, Qinghai Province

The discovery of Gonghe County Haergeng tungsten polymetallic deposit is one of the most important tungsten deposits discovered in Qinghai Province in recent years.It has important theoretical and practicalsignificance.1 Regional geological background The research area lies in the northwest edge of

Evolution and prospect of China's rural development policy: A policy text analysis of the No.1 Central Documents

By combing 20 documents of the Central Committee on the historical evolution of rural development policies since 1982, we hold that historical evolution has undergone reforms, adjustments, modernization developments and new ideas, and the path of reform experienced economic recovery, industrial nurturing agriculture, agriculture modernization and rural revitalization. The study found that: farmers' income has always been the focus of attention; agricultural production has shifted from total demand to green ecology; urban and rural resource elements are not well-organized, resulting in internal contradictions. The implementation of the rural revitalization strategy is an important measure to fundamentally solve the rural development problems in the new era.

Regional Distribution and Prospects of Potash in China

China was formed by amalgamation of several small continental blocks （cratons）, micro, blocks and orogenic belts in different paleoclimatic settings. It may be correlated with other continental blocks but has its own specific characteristics; therefore the tectonic environments of China＇s marine and continental saline basins and salt, and potash, forming environment have some specific characteristics： multiple phases of salt formation, difference in salt, forming ages, migration and concentration of salt, forming processes and diversity of component materials, as well as small sizes of marine saline basins and great changes of saline basins in the late stage and occurrence of abundant liquid mineral resources. The nature of the tectonic basement exerted a key controlling effect on the formation of potash basins. The stable tectonic region was favorable for potash concentration in a quasi, stable region, and quasi, and the quasi-stable region was favorable for salt concentration and potash formation in a local stable tectonic region. Most China＇s major ancient saline basins occur in ＂quasi, cratons （continental block）＂; especially all the marine saline basins occur in continental blocks with the Precambrian basement. These regions are the key ones for potash search. Most relatively large, scale soluble salt deposits are developed in relatively stable continental nuclei. According to the characteristics of the tectonic domains where China＇s salt, forming basins are located, the North China, Yangtze and Tarim, Qaidam salt minerogenetic domains and the northern Qiangtang, western Yunnan salt minerogenetic belt may be distinguished. Their salt and potash prospects will be discussed separately.

Research Hotspots and Prospects of Third-Tier Construction Industrial Heritage

From the perspective of bibliometric analysis, based on the data of 286 related literature retrieved and screened in the CNKI database, coOC software is used for data processing, and the relevant knowledge graphs such as the amount of publication, authors, and keywords in the field of third-line heritage research are plotted. The results show that the attention to third-line heritage research in China is increasing day by day, and the overall volume of publications shows a rapid growth trend;The authors’ clusters are scattered and do not form a broad and close network of cooperation;The research hotspots focus on the protection and reuse of third-line heritage, and the quantitative research methods are insufficient. In the future, the breadth and depth of third-line heritage research need to be expanded.

Hydrocarbon Prospectivity and Risk Assessment of “Bob” Field Central Swamp Depobelt, Onshore Niger Delta Basin, Nigeria

This paper evaluates the hydrocarbon prospectivity and play risks of “Bob” field in Niger Delta Basin, Nigeria. The aim is to enhance exploration success through improved approach/technique by incorporating risk analysis that previous studies have not fully considered. This approach combines a set of analyses including stratigraphic/structural, amplitude, petrophysical parameter, volumetric and play risk using a suite of well logs and 3D seismic data. Maximum amplitude anomaly map extracted on the surfaces of delineated 3 reservoirs revealed 6 prospects, namely: Dippers, Cranes, Turacos, Nicators, Jacanas and Pelicans with hydrocarbon accumulation. Petrophysical analysis showed ranges of values for porosity, permeability and water saturation of 0.21 to 0.23, 158.96 to 882.39 mD, and 0.07 to 0.11, respectively. The various prospects yielded the following stock tank volumes 12.73, 6.84, 3.84, 11.32, 7.42 and 4.76 Million barrels (Mbls) each respectively in a column of 66 ft reservoir sand in the study area. Play risk analysis results gave: Pelicans and Nicators (low), Turacos and Dippers (moderate), while Jacanas and Cranes show high risk with minimal promise for good oil accumulation. The prospects possess good reservoir petrophysical properties with low to moderate risk, thus, viable for commercial hydrocarbon production, which increases confidence in management decisions for production.

基于靴襻渗流模型的国际信息通信产业贸易前景分析

信息通信技术(ICT)产业是国际数字经济发展的核心产业,交互贯通的国际ICT产业贸易网络能够客观反映出ICT产业贸易的时空分异格局。本文运用复杂网络分析方法探测国际ICT产业贸易网络的基础拓扑特征及中心性特征,同时运用靴襻渗流模型讨论ICT产业贸易的危机传播过程。研究发现,国际ICT产业整体贸易强度有所扩大,呈现出明显的区域集中化趋势,但贸易联系的整体活跃度、贸易范围和网络传输效率都明显降低;主要ICT产业贸易国(地区)整体的危机传染力高于以往,防控核心贸易国(地区)的点危机与预防核心板块的区域性联动危机相结合将是阻止危机全球蔓延的有效举措。

我国历史文化村落保护研究的内容综述与工作前瞻

历史文化村落既是历史文化遗产保护的核心要素,又是我国乡村振兴战略推进的工作对象。文章借助CiteSpace分析工具,对相关文献进行关键信息的检索、提取、分析和研判。分析结果表明,我国历史文化村落研究大体可划分为依附研究、专属研究和拓展研究3个阶段,主要围绕“本体机理的认知解读”“文化遗产的保护转型”“社会体系的组织治理”和“产业发展的创新规划”4大主题展开讨论,体现从“实体历史空间”到“复合多元要素”的对象演变、从“孤立对象保护”到“区域统筹发展”的目标拓展、从“单科定性”到“交叉定量”的方法进化等研究特征。最后,面对乡村振兴的机遇与乡村收缩的挑战,从研究视角、保护理论、实践方法等方面提出工作展望,以期为新时代我国历史文化村落保护实践提供科学的理论支持。

“双创”培养模式驱动下食品质量与安全专业人才培养需求分析

在健康中国的战略背景下,食品质量与安全行业的就业环境发生了巨大变化,对创新创业型高素质人才的需求不断增加。该文通过分析双创教育在高等教育和经济社会发展中的重要作用,特别是双创教育对食品质量与安全专业的重要性及不足分析,进一步探讨双创教育对食品质量与安全专业人才培养方向和发展前景。

近15年来我国耕读教育研究:热点、趋势与展望——基于Citespace的文献计量分析

耕读教育是从我国传统耕读文化中发展演变来的一种教育理念和方式,能有效助推乡村振兴和涉农院校人才培养质量的提高。以CNKI收录的近15年来关于耕读教育的文献为数据源,借助Citespace6.1.R6可视化分析软件进行计量分析,发现耕读教育研究经过了初步探索、稳步发展和迅速发展阶段,总体呈稳步增长态势。而且,研究主体间已形成初步合作网络;研究主题宽泛,涉及耕读文化、思想、人物等;研究热点集中在耕读文化观念传承和价值挖掘、耕读教育价值探讨和路径探析、耕读育人体系模式探讨等方面;研究存在主体合作分散、内容同质化严重、方法单一等问题。未来研究应重视拓宽研究角度、深化研究内容,加强学科联系、探索融合研究,整合研究团队、搭建研究平台,发挥涉农高校研究主体作用、共建高质量耕读实践育人体系。

厚规格板材异步和蛇形轧制分析及研究综述

随着钢铁行业和工业技术的飞速发展,各种规格和不同种类的板材相继出现,蛇形轧制技术在其中起到了重要的作用,它是为解决厚规格板材异步轧制复合弯曲问题发展而来的一种新型轧制工艺。自2001年蛇形轧制出现以来,受到了国内外专家学者和各种钢厂的广泛关注。为了使读者对蛇形轧制技术及应用能够有更加全面的了解并深入认识到新型工艺的发展前景,文中首先介绍了异步轧制和蛇形轧制工艺技术,给出了蛇形轧制在应用领域的优势所在;然后从蛇形轧制的基本原理着手,将理论与实践有机结合,进而重点概述了蛇形轧制在应变、弯曲曲率、轧制力以及力学性能和组织性能机理分析三个方面的国内外研究现状;最后,总结分析了当前蛇形轧制技术在工业中的应用发展前景,为新一代热机械控制加工工艺的革新提供方向指导。

基于知识图谱的我国农业数字化研究现状与未来展望

以CNKI数据库中主题为“农业数字化”的相关文献作为数据来源,运用CiteSpace软件对农业数字化领域研究现状进行可视化分析。结果表明,农业数字化领域研究热度日趋提升,文献数量在2020年呈现井喷式增长,并逐渐建立起较有凝聚力的科研群体;该领域研究热点不断拓展,从农业机械、农业信息化等延申至数字农业、数字技术、智慧农业等方面;数字技术成为该领域今后研究的重点方向。最后,未来农业数字化发展仍面临着整体水平不高、区域发展不平衡、基础设施薄弱等问题需进一步探索与完善。

文化翻译视角下壮族体育文化外宣翻译文献分析与研究展望

随着我国综合实力和国际地位不断攀升,民族自信和文化自信日益受到尊崇。梳理近20年文献综述发现,现阶段壮族体育文化的研究成果虽初具规模,但大多集中在溯源、挖掘、整理、传承发展等方面,在外宣方面的研究较少,且不成体系,文化表达、翻译策略等方面存在短板。该文在文化翻译理论指导下,一方面,通过对相关文献的梳理,发现研究短板,提出研究建议与展望。另一方面,为探讨如何选取适宜的外宣翻译策略,即如何保持壮族特色又能提高文化传播适应性的研究而提供研究依据,从而加快广西民族特色文化的传播,使得壮族体育文化能更好地造福人类健康。