Raman spectroscopy as process analytical tool in downstream processing of biotechnology

Downstream processing or product recovery plays a vital role in the development of bioprocesses.To improve the bioprocess efficiency,some unconventional methods are much required.The continuous manufacturing in downstream processing makes the Process Analytical Technologies(PATs)as an important tool.Monitoring and controlling bioprocess are an essential factor for the principles of PAT and quality by design.Spectroscopic methods can apply to monitor multiple analytes in real-time with less sample processing with significant advancements.Raman spectroscopy is an extensively used technique as an analytical and research tool owing to its modest process form,non-destructive,non-invasive optical molecular spectroscopic imaging with computer-based analysis.Generally,its application is essential for the analysis and characterization of biological samples,and it is easy to operate with minimal sample.The innovation on various types of enhanced Raman spectroscopy was designed to enhance the Raman analytical technique.Raman spectroscopy could couple with chemometrics to provide reliable alternative analysis method of downstream process analysis.Thus,this review aims to provide useful insight on the application of Raman spectroscopy for PAT in downstream processing of biotechnology and Raman data analysis in biological fields.

DECISION-MAKING OF SLICING SCHEME IN FUSED DEPOSITION MODELING PROCESS BASED ON ANALYTICAL HIERARCHICAL PROCESS

Based on analyzing the influences of a slicing scheme on stair-stepping effect, supporting structure, efficiency and deformation, etc. , analytical hierarchical process （AHP） combining with fuzzy synthetic evaluation is introduced to make decision in slicing schemes for a processing part. The application in determining the slicing scheme for a computer mouse during prototyping shows that the method increases the rationality during decision- making and improves quality and efficiency for the prototyping part.

Hazard assessment of landslide disaster using information value method and analytical hierarchy process in highly tectonic Chamba region in bosom of Himalaya

The present study is focused on a comparative evaluation of landslide disaster using analytical hierarchy process and information value method for hazard assessment in highly tectonic Chamba region in bosom of Himalaya. During study, the information about the causative factors was generated and the landslide hazard zonation maps were delineated using Information Value Method(IV) and Analytical Hierarchy Process(AHP) using Arc GIS(ESRI). For this purpose, the study area was selected in a part of Ravi river catchment along one of the landslide prone Chamba to Bharmour road corridor of National Highway(NH^(-1)54 A) in Himachal Pradesh, India. A numeral landslide triggering geoenvironmental factors i.e. slope, aspect, relative relief, soil, curvature, land use and land cover(LULC), lithology, drainage density, and lineament density were selected for landslide hazard mapping based on landslide inventory. Landslide hazard zonation map was categorized namely "very high hazard, high hazard, medium hazard, low hazard, and very low hazard". The results from these two methods were validated using Area Under Curve(AUC) plots. It is found that hazard zonation map prepared using information value method and analytical hierarchy process methods possess the prediction rate of 78.87% and 75.42%, respectively. Hence, landslide hazardzonation map obtained using information value method is proposed to be more useful for the study area. These final hazard zonation maps can be used by various stakeholders like engineers and administrators for proper maintenance and smooth traffic flow between Chamba and Bharmour cities, which is the only route connecting these tourist places.

Landslide susceptibility analysis of Karakoram highway using analytical hierarchy process and scoops 3D

Landslides are prevalent,regular,and expensive hazards in the Karakoram Highway(KKH)region.The KKH connects Pakistan with China in the present China-Pakistan Economic Corridor(CPEC)context.This region has not only immense economic importance but also ecological significance.The purpose of the study was to map the landslide-prone areas along KKH using two different techniquesAnalytical Hierarchy Process(AHP)and Scoops 3 D model.The causative parameters for running AHP include the lithology,presence of thrust,land use land cover,precipitation,and Digital Elevation Model(DEM)derived variables(slope,curvature,aspect,and elevation).The AHP derived final landslide susceptibility map was classified into four zones,i.e.,low,moderate,high,and extremely high.Over 80%of the study area falls under the moderate(43%)and high(40%)landslide susceptible zones.To assess the slope stability of the study area,the Scoops 3 D model was used by integrating with the earthquake loading data.The results of the limit equilibrium analysis categorized the area into four groups(low,moderate,high,and extremely high mass)of slope failure.The areas around Main Mantle Thrust(MMT)including Dubair,Jijal,and Kohistan regions,had high volumes of potential slope failures.The results from AHP and Scoops 3 D techniques were validated with the landslides inventory record of the Geological Survey of Pakistan and Google Earth.The results from both the techniques showed similar output that coincides with the known landslides areas.However,Scoops 3 D provides not only susceptible zones but also the range of volume of the potential slope failures.Further,these techniques could be used in other mountainous areas,which could help in the landslide mitigation measures.

Seismic risk evaluation for a planning mountain tunnel using improved analytical hierarchy process based on extension theory

Seismic risk evaluation(SRE) in early stages(e.g., project planning and preliminary design)for a mountain tunnel located in seismic areas has the same importance as that in final stages(e.g.,performance-based design, structural analysis, and optimization). SRE for planning mountain tunnels bridges the gap between the planning on the macro level and the design/analysis on the micro level regarding the risk management of infrastructural systems. A transition from subjective or qualitative description to objective or quantitative quantification of seismic risk is aimed to improve the seismic behavior of the mountain tunnel and thus reduce the associated seismic risk. A new method of systematic SRE for the planning mountain tunnel was presented herein. The method employs extension theory(ET)and an ET-based improved analytical hierarchy process. Additionally, a new risk-classification criterion is proposed to classify and quantify the seismic risk for a planning mountain tunnel. This SRE method is applied to a mountain tunnel in southwest China, using the extension model based on matter element theory and dependent function operation.The reasonability and flexibility of the SRE method for application to the mountain tunnel are illustrated.According to different seismic risk levels and classification criteria, methods and measures for improving the seismic design are proposed, which can reduce the seismic risk and provide a frame of reference for elaborate seismic design.

Material selection of polymeric composite automotive bumper beam using analytical hierarchy process

Selection of materials,as an area of design research,has been under considerable interest over the years.Materials selection is one of the most important activities in the product development process.Inappropriate decision of materials can cause the product to be reproduced or remanufactured.To avoid this circumstance,one of the useful tools that can be employed in determining the most appropriate material is analytical hierarchy process(AHP).To illustrate the application of AHP,six different types of composite materials were considered.The most appropriate one for suitability of use in manufacturing automotive bumper beam was determined by considering eight main selection factors and 12 sub-factors.The AHP analysis reveals that the glass fibre epoxy is the most appropriate material because it has the highest value(25.7%,mass fraction) compared with other materials.The final material is obtained by performing six different scenarios of the sensitivity analysis.It is proved that glass fibre epoxy is the most optimum decision.

Mapping favorable groundwater potential recharge zones using a GIS-based analytical hierarchical process and probability frequency ratio model:A case study from an agro-urban region of Pakistan

In Punjab(Pakistan),the increasing population and expansion of land use for agriculture have severely exploited the regional groundwater resources.Intensive pumping has resulted in a rapid decline in the level of the water table as well as its quality.Better management practices and artificial recharge are needed for the development of sustainable groundwater resources.This study proposes a methodology to delineate favorable groundwater potential recharge zones(FPRI)by integrating maps of groundwater potential recharge index(PRI)with the DRASTIC-based groundwater vulnerability index(VI).In order to evaluate both indexes,different thematic layers corresponding to each index were overlaid in ArcGIS.In the overlay analysis,the weights(for various thematic layers)and rating values(for sub-classes)were allocated based on a review of published literature.Both were then normalized and modified using the analytical hierarchical process(AHP)and a frequency ratio model respectively.After evaluating PRI and FPRI,these maps were validated using the area under the curve(AUC)method.The PRI map indicates that 53%of the area assessed exists in very low to low recharge zones,22%in moderate,and 25%in high to excellent potential recharge zones.The VI map indicates that 38%of the area assessed exists in very low to low vulnerability,33%in moderate,and 29%in high to very high vulnerability zones.The FPRI map shows that the central region of Punjab is moderately-to-highly favorable for recharge due to its low vulnerability and high recharge potential.During the validation process,it was found that the AUC estimated with modified weights and rating values was 79%and 67%,for PRI and VI indexes,respectively.The AUC was less when evaluated using original weights and rating values taken from published literature.Maps of favorable groundwater potential recharge zones are helpful for planning and implementation of wells and hydraulic structures in this region.

Sustainability Assessment and Ranking of Run of the River(RoR) Hydropower Projects Using Analytical Hierarchy Process(AHP):A Study from Western Himalayan Region of India

In the present scenario,tapping the unutilised hydropower potential is one of the highest priorities in developing countries of the world.Special emphasis is being imparted to run of the river(RoR)mode of power generation.However,the governments are now facing the dilemma whether to promote small hydropower projects(SHPs) or encourage large hydropower projects(LHPs).RoR large hydropower projects result into large scale cutting of mountains for constructing tunnels and access roads,generation of huge quantity of muck and large scale impact on flora and fauna due to diversion of rivers/streams.On the other hand,though SHPs are claimed to be greener and more sustainable by a section of researchers and energy planners but,they will be required to be set up in large number to generate equivalent amount of electricity.The aim of this study is to rank the most sustainable installed capacity range of RoR hydropower projects.To achieve this aim,the study proposes the use of quite popular multi-criteria decision making(MCDM)method of Operation Research named Analytical Hierarchy Process.A case study has been presented from Himachal Pradesh,a hydro rich state located in the western Himalayan region.As per sustainability assessment carried out in this study,hydropower projects in the capacity range 1 to 5 MW have been ranked to be the most sustainable.

Analysis of explosion risk factor potential on coal reclaim tunnel facilities by modified analytical hierarchy process

This study focused on developing a risk assessment method for explosion at a coal reclaim tunnel （CRT） facility. The method was developed based on an analytical hierarchy process （AHP）, which is an expert system that quantifies the factors of explosion incidents, based on events and hierarchies. In this paper, the proposed model was modification from original AHP model, specifically modifying the structure from ＂alternative＇s results＂ to ＂total risk-rating＇s results＂. The total risk-rating is obtained by summing up risk-rating of each factor, where the risk-rating is a multiplication product of the risk value by the AHP weighted value. To support decision-making using the expert system, data on the real conditions of the CRT were collected and analyzed. A physical modeling of the CRT with laboratory-scale experiments was carried out to show the impact of a ventilation system in CRT on diluting the methane gas and coal dust, in order to support the quantification of AHP risk value. The criteria to evaluate the risk of explosion was constructed from six components that are： fuel, oxygen, ignition, confinement, dispersion, and monitoring system. Those components had fifty-two factors that serve as sub-components （root causes）. The main causes of explosion in CRT were found to be： mechanical ventilation failure and abnormal ventilation, breakdown of monitoring system, and coal spontaneous-combustion. Assessments of two CRT facilities at Mine A and Mine B were carried out as a case study in order to check the reliability of the developed AHP method. The results showed that the risk rating of Mine A was classified as high and Mine B was classified as medium, which is in a good agreement with the site conditions.

A Genetic Fuzzy Analytical Hierarchy Process Based Projection Pursuit Method for Selecting Schemes of Water Transportation Projects

The optimal selection of schemes of water transportation projects is a process of choosing a relatively optimal scheme from a number of schemes of water transportation programming and management projects, which is of importance in both theory and practice in water resource systems engineering. In order to achieve consistency and eliminate the dimensions of fuzzy qualitative and fuzzy quantitative evaluation indexes, to determine the weights of the indexes objectively, and to increase the differences among the comprehensive evaluation index values of water transportation project schemes, a projection pursuit method, named FPRM-PP for short, was developed in this work for selecting the optimal water transportation project scheme based on the fuzzy preference relation matrix. The research results show that FPRM-PP is intuitive and practical, the correction range of the fuzzy rained is both stable and accurate; preference relation matrix A it produces is relatively small, and the result obtherefore FPRM-PP can be widely used in the optimal selection of different multi-factor decision-making schemes.

Alunite processing method selection using the AHP and TOPSIS approaches under fuzzy environment

Alunite is the most important non bauxite resource for alumina. Various methods have been proposed and patented for processing alunite, but none has been performed at industrial scale and no technical,operational and economic data is available to evaluate methods. In addition, selecting the right approach for alunite beneficiation, requires introducing a wide range of criteria and careful analysis of alternatives.In this research, after studying the existing processes, 13 methods were considered and evaluated by 14 technical, economic and environmental analyzing criteria. Due to multiplicity of processing methods and attributes, in this paper, Multi Attribute Decision Making methods were employed to examine the appropriateness of choices. The Delphi Analytical Hierarchy Process(DAHP) was used for weighting selection criteria and Fuzzy TOPSIS approach was used to determine the most profitable candidates. Among 13 studied methods, Spanish, Svoronos and Hazan methods were respectively recognized to be the best choices.

Landfill Siting Using GIS and AHP （Analytical Hierarchy Process）： A Case Study AI-Qasim Qadhaa, Babylon, Iraq

The selection of a landfill site is considered as a complicated task because this process is based on many factors and restrictions. For Al-Qasim Qadhaa, which is situated in the southern part of the Babylon Governorate, Iraq, there is no landfill site in that area that conforms to the scientific criteria for selecting sites for landfill. For this reason, 15 criteria were adopted in this study （groundwater depth, rivers, soil types, agriculture lands use, land use, elevation, slope, gas pipelines, oil pipelines, power lines, roads, railways, urban centers, villages and archaeological sites） using GIS （geographic information system）, which has a large ability to manage input data. In addition, the AHP （analytical hierarchy process） method was used to derive the relative weightings for each criterion using pair-wise comparison. To obtain the suitability index for candidate landfill sites, a weighted linear combination method was used. After combining these methods, two suitable candidate landfill sites, with areas of 2.766 km2 and 2.055 km2, respectively, were found to satisfy the scientific and environmental requirements. The area of these sites can accommodate solid waste from 2020 until 2030 based on the required area, which was 0.702 km2.

UV/Vis-based process analytical technology to improve monoclonal antibody and host cell protein separation

Process analytical technology(PAT) is gaining more interest in the biomanufacturing industry because of its potential to improve operational control and compliance through real-time quality assurance.Currently, biopharmaceutical producers mainly monitor chromatographic processes with ultraviolet/visible(UV/Vis) absorbance. However, this measurement has a very limited correlation with purity and quantity. The current study aims to determine the concentration of monoclonal antibody(mAb) and host cell proteins(HCPs) using a build-in UV/Vis monitoring during Protein A affinity chromatography and to optimize the separation conditions for high purity of mAb and minimizing the HCPs content. The eluate was analyzed through in-line UV/Vis at 280 and 410 nm, representing mAb and HCPs concentration,respectively. Each 0.1 column volume(CV) fraction of UV/Vis chromatogram peak area were calculated,and different separation conditions were then compared. The optimum conditions of mAb separation were found as 12 CV loading, elution at pH 3.5, and starting the collection at 0.5 CV point, resulting in high m Ab recovery of 95.92% and additional removal of 49.98% of HCP comparing with whole elution pool. This study concluded that UV/Vis-based in-line monitoring at 280 and 410 nm showed a high potential to optimize and real-time control Protein A affinity chromatography for mAb purification from HCPs.

Study progression in application of process analytical technologies on film coating

Film coating is an important unit operation to produce solid dosage forms,thereby,the monitoring of this process is helpful to find problems in time and improve the quality of coated products.Traditional methods adopted to monitor this process include measurement of coating weight gain,performance of disintegration and dissolution test,etc.However,not only do these methods cause destruction to the samples,but also consume time and energy.There have recently emerged the applications of process analytical technologies(PAT)on film coating,especially some novel spectroscopic and imaging technologies,which have the potential to real-time track the progress in film coating and optimize production efficiency.This article gives an overview on the application of such technologies for film coating,with the goal to provide a reference for the further researches.

Application of GIS based analytical hierarchy process and multicriteria decision analysis methods to identify groundwater potential zones in Jedeb Watershed, Ethiopia

The hydrogeological situation of the study area requires the identification of groundwater potential.Remote sensing and satellite data have proven to be reliable tools for understanding various factors that affect groundwater occurrence and movement.This study employed weighted overlay analysis based on satellite imagery and secondary data to create a thematic map for characterizing groundwater potentials in the study area located within Abbay Basin,Ethiopia.Remote sensing(RS)and GIS-based Fuzzy-Analytical Hierarchy Process methods were utilized to classify groundwater potential(GWP)zones into five categories:Very good,good,moderate,poor,and very poor.The central and eastern parts of the study area were identified as having high(33.186%)and very high(2.351%)groundwater potentials,while the western part exhibited poor and very poor potential areas.The groundwater potential map delineated higher and moderate potentials,suitable for installing shallow and production bores.This research demonstrates the effectiveness of RS and GIS techniques for delineating groundwater potential zones,which can aid in the planning and management of groundwater resources.The research findings have the potential to contribute to the formulation of improved groundwater management programs in the study area.

Fuzzy comprehensive evaluation of bridge quality based on analytical hierarchy process

Bridge quality assessment is an important part in the final acceptance of new bridge construction,and it is also the main basis for the reinforcement or removal of old bridges.We evaluated the weight of each affecting factor to the upper events using progressive analytical hierarchy process(AHP)with the adoption of 3 scaling,reduced the calculation in analytical process,and precluded the nonuniformity of the scaling system.We obtained a comprehensive evaluation system of bridge quality, and verified its pra...

Applying Analytical Hierarchy Process to a WiMAX Performance Evaluation Model

Evaluating performance of individual features of WiMAX technology is a topic of widespread discussion. Currently, there is no quantitative way of measuring WiMAX technology so that wireless operators can meet their design objectives. This paper outlines a set of design criteria for WiMAX and provides a decision-making aid that ranks the importance of criteria using Analytic Hierarchy Process （AHP）. This ranking should sufficiently reflect market expectations of the relative importance of various design criteria. A model integrating AHP priorities with enhanced Data Envelopment Analysis （DEA） is the basis for formulating a technological value in simple, comparable format. A case study is provided to show how this technological value is used to evaluate a three year network deployment plan. In the future, this model could be extended to WiMAX equipment suppliers for the purpose of validating performance targets of individual criteria, and enhancing supplier roadmaps for future network development.

Dif？ culty Discrimination of Interpretation Teaching Materials based on Analytic Hierarchy Process and Natural Language Processing

Difficulty discrimination is an important step in autonomous design and interpreting teaching materials development, which is related to scientifi c nature of the materials, teaching effectiveness, and sequential teaching progress. In this paper, we focus on the diffi culty discrimination of interpretation teaching materials on the basis of analytic hierarchy process and natural language processing. We analyze several factors which affect interpretation teaching materials, and we introduce theories of analytic hierarchy process and natural language processing which is intuitive and credible operation basis.

Modification of Intensive Care Unit Data Using Analytical Hierarchy Process and Fuzzy C-Means Model

This paper proposes a proper methodology in data modification by using AHP （analytical hierarchy process） technique and FCM （fuzzy c-mean） model in the ICU （intensive care unit）. The binary data were created from continuous data using FCM model, while the continuous data were constructed from binary data using AHP technique. The models used in this study are FCRM （fuzzy c-regression model）. A case study in scale of health at the ICU ward using the AI-IP, FCM model and FCRM models was conducted. There are six independent variables in this study. There are four cases which are considered as the result of using AHP technique and FCM model against independent data. After comparing the four cases, it was found that case 4 appeared to be the best model, because it has the lowest MSE （mean square error） value. The original data have the MSE value of 97.33, while the data in case 4 have the MSE value of 82.75. This means that the use of AHP technique can reduce the MSE value, while the use of FCM model can not reduce the MSE value. In other words, it can be proved that the AHP technique can increase the accuracy of prediction in modeling scale of health which is associated with the mortality rate in the ICU.

Analytical fits to the synchrotron functions

Accurate fitting formulae to the synchrotron function, F（x）, and its com- plementary function, G（x）, are performed and presented. The corresponding relative errors are less than 0.26% and 0.035% for F（x） and G（x）, respectively. To this end we have, first, fitted the modified Bessel functions, Ks/3（x） and K2/3（x）. For all the fitted functions, the general fit expression is the same, and is based on the well known asymptotic forms for low and large values of z for each function. It consists of multi- plying each asymptotic form by a function that tends to unity or zero for low and large values of z. Simple formulae are suggested in this paper, depending on adjustable parameters. The latter have been determined by adopting the Levenberg-Marquardt algorithm. The proposed formulae should be of great utility and simplicity for com- puting spectral powers and the degree of polarization for synchrotron radiation, both for laboratory and astrophysical applications.