The serum of foxes and red deer from the Province of Soria (northern Spain) was screened in indirect immunofluorescence assays to determine whether these animals could be used as sentinels of the tick-borne pathogens ...The serum of foxes and red deer from the Province of Soria (northern Spain) was screened in indirect immunofluorescence assays to determine whether these animals could be used as sentinels of the tick-borne pathogens Anaplasma phagocytophilum and Ehrlichia chaffeensis. The results suggest that foxes and red deer would not make good sentinels in epidemiological studies on E. chaffeensis in this region, although red deer could be used as such for the study of A. phagocytophilum transmission.展开更多
Objective Lyme disease and Human granulocytic anaplasmosis are tick-borne diseases caused by Borrelia burgdorferi and Anoplasrna phagocytophilum respectively. We have investigated infection and co-infection of the two...Objective Lyme disease and Human granulocytic anaplasmosis are tick-borne diseases caused by Borrelia burgdorferi and Anoplasrna phagocytophilum respectively. We have investigated infection and co-infection of the two diseases in the population of forest areas of eight provinces in China by measuring seroprevalence of antibodies against B. burgdorferi and A. phagocytophilum. Methods Forest areas in 8 provinces were chosen for investigation using whole sampling and questionnaire survey methods. 3 669 serum samples from people in the forest areas were tested for the presence of antibodies by indirect immunofluorescent assay （IFA）. Results Seroprevalence against B. burgdorferi was 3% to 15% and against A. phagocytophilum was 2% to 18% in the study sites in the 8 provinces in China. We also found co-infection of B. burgdorferi and A. phagocytophilum in 7 of the 8 provinces （the exception being the Miyun area in Beijing）. The seroprevalence for both B. burgdorferi and A. phagocytophilum was significantly higher among people exposed to ticks than among people who were not exposed to ticks. Conclusion We conclude that both pathogens are endemic in the forest areas in the eight provinces, but the prevalence of B. burgdorferi and A. phagocytophilum differs between the provinces.展开更多
目的确定发热伴血小板减少综合征患者中是否存在人粒细胞无形体病(human granulocytic anaplasmosis,HGA),并进行HGA临床分析。方法将2010年收治的42例发热伴血小板减少综合征患者血液标本,送中国疾病预防控制中心(Centers for Disease ...目的确定发热伴血小板减少综合征患者中是否存在人粒细胞无形体病(human granulocytic anaplasmosis,HGA),并进行HGA临床分析。方法将2010年收治的42例发热伴血小板减少综合征患者血液标本,送中国疾病预防控制中心(Centers for Disease Control and Prevention,CDC)进行急性期与恢复期血清IgG抗体检测,部分标本送山东省CDC进行巢式PCR扩增患者血液DNA无形体16SrRNA基因。对实验室确诊的HGA进行临床特点分析。结果经血清学实验室结果证实HGA5例,PCR结果证实2例。其中1例同时PCR检测新型布尼亚病毒抗体阳性。结论发热伴血小板减少综合征病例中存在HGA。临床应注意加强对2类疾病的鉴别并分别选择必要的抗生素治疗。展开更多
文摘The serum of foxes and red deer from the Province of Soria (northern Spain) was screened in indirect immunofluorescence assays to determine whether these animals could be used as sentinels of the tick-borne pathogens Anaplasma phagocytophilum and Ehrlichia chaffeensis. The results suggest that foxes and red deer would not make good sentinels in epidemiological studies on E. chaffeensis in this region, although red deer could be used as such for the study of A. phagocytophilum transmission.
文摘为了利用生物信息学预测嗜吞噬细胞无形体ZJ-msp4蛋白的优势抗原表位,给寻找无形体病诊断和预防的候选抗原表位提供参考,试验参照Gen Bank中的msp4基因序列(登录号为EU008082.1)进行生物信息学分析。结果表明:ZJ-msp4蛋白的分子质量为30.1 ku,理论等电点为5.71,该蛋白的不稳定系数为29.13,组成较多的氨基酸有丝氨酸(Ser,13.8%)、甘氨酸(Gly,9.2%)、丙氨酸(Ala,8.9%)等;二级结构预测ZJ-msp4蛋白主要以无规卷曲、延伸链、α螺旋为主,TMHMM2.0预测该蛋白的第7~29位氨基酸可能为跨膜区,该跨膜区可能为信号肽序列;Signal P 4.1预测在msp4蛋白的N端第1~29位氨基酸序列为信号肽序列,该分析结果与TMHMM2.0分析结果一致;B细胞表位预测该msp4蛋白有11个线性表位,其中以28~38位、64~77位、87~101位、134~143位、245~264位是ZJ-msp4蛋白的优势抗原表位区段,可以作为诊断无形体病的候选表位。
基金supported by the 12th Five-Year Major National Science and Technology Projects of China (No. 2012ZX10004219-007) and ( No. 2013ZX10004001)
文摘Objective Lyme disease and Human granulocytic anaplasmosis are tick-borne diseases caused by Borrelia burgdorferi and Anoplasrna phagocytophilum respectively. We have investigated infection and co-infection of the two diseases in the population of forest areas of eight provinces in China by measuring seroprevalence of antibodies against B. burgdorferi and A. phagocytophilum. Methods Forest areas in 8 provinces were chosen for investigation using whole sampling and questionnaire survey methods. 3 669 serum samples from people in the forest areas were tested for the presence of antibodies by indirect immunofluorescent assay （IFA）. Results Seroprevalence against B. burgdorferi was 3% to 15% and against A. phagocytophilum was 2% to 18% in the study sites in the 8 provinces in China. We also found co-infection of B. burgdorferi and A. phagocytophilum in 7 of the 8 provinces （the exception being the Miyun area in Beijing）. The seroprevalence for both B. burgdorferi and A. phagocytophilum was significantly higher among people exposed to ticks than among people who were not exposed to ticks. Conclusion We conclude that both pathogens are endemic in the forest areas in the eight provinces, but the prevalence of B. burgdorferi and A. phagocytophilum differs between the provinces.
文摘目的确定发热伴血小板减少综合征患者中是否存在人粒细胞无形体病(human granulocytic anaplasmosis,HGA),并进行HGA临床分析。方法将2010年收治的42例发热伴血小板减少综合征患者血液标本,送中国疾病预防控制中心(Centers for Disease Control and Prevention,CDC)进行急性期与恢复期血清IgG抗体检测,部分标本送山东省CDC进行巢式PCR扩增患者血液DNA无形体16SrRNA基因。对实验室确诊的HGA进行临床特点分析。结果经血清学实验室结果证实HGA5例,PCR结果证实2例。其中1例同时PCR检测新型布尼亚病毒抗体阳性。结论发热伴血小板减少综合征病例中存在HGA。临床应注意加强对2类疾病的鉴别并分别选择必要的抗生素治疗。
