Effect of Different Disinfection Methods on Calluses of Amorphophallus albus P.Y.Liu&J.F.Chen

This study aimed to examine the effect of different disinfection methods on the callus of Amorphophallus albus in the tissue culture and rapid propagation system.The calluses induced from buds of A.albus were disinfected with benzalkonium bromide,mercury bichloride,sodium hypochlorite,and ethanol,respectively,for different time periods.The growth of calluses was compared between different treatments and observed in detail by dissection.The results showed that the disinfection with 0.1% mercury bichloride for 5 min and 75% ethanol for 5 s had mild effect on the callus,with the lowest browning rates of 16.32% and 15.46% and the lowest vitrification rates of 3.24% and 9.72%,respectively,after 20 d.The disinfection with benzalkonium bromide and sodium hypochlorite had strong effect on the callus cells,with the lowest browning rate of 50.69% and 25.12% and the lowest vitrification rates of 28.47% and 28.43%,respectively.The cytological observation result showed that the calluses disinfected with ethanol and mercury bichloride showcased compact structures,while those disinfected with sodium hypochlorite and benzalkonium bromide exhibited loose structures.To sum up,it is recommended that 75% ethanol or 0.1% mercury bichloride can be used for disinfection in the tissue culture and rapid propagation of A.albus,while the use of benzalkonium bromide and sodium hypochlorite should be avoided as much as possible.

Anatomical berry characteristics during the development of grape berries with different shapes

The aim of this study was to characterize the changes in berry anatomy during the development of grape(Vitis vinifera L.,and Vitis vinifera×Vitis labrusca)with different shapes.Paraffin sectioning was used to examine the structural parameters of the cells.The results revealed that,with the development of berries,the transverse and longitudinal diameters of the flesh cells gradually increased,revealing certain regularity.However,the transverse and longitudinal diameters of the epidermal and sub-epidermal cells were different between varieties,reflecting the specificity of the varieties.The growth of the transverse and longitudinal diameters of the berries was found to be completed in the early stage of development.A combination of correlation analysis and size analysis for each cell layer revealed that,due to the small crosssectional area of the epidermal and sub-epidermal cells,the influence of these cells on the transverse and the longitudinal diameters of the berries would be small.In conclusion,the longitudinal and transverse diameters of the grape berries were mainly determined by the longitudinal and transverse diameters of the flesh cells.The different shapes of the grape berries could mainly be attributed to the different growth rates of the flesh cells in the longitudinal and transverse directions.These different rates of growth led to different lengths and widths of the berry.