To apply gene therapy to breast carcinoma wepropose a model for in vitro gene transfer of the herpessimplex thymidine kinase (HSV-tk) gene using Stkvector-producing cells. In this study the HSV-tk gene wastransferred ...To apply gene therapy to breast carcinoma wepropose a model for in vitro gene transfer of the herpessimplex thymidine kinase (HSV-tk) gene using Stkvector-producing cells. In this study the HSV-tk gene wastransferred to mouse mammary carcinoma cell line 4Tlwith the method of infection. The HSV-tk gene in thegenome of transduced cells (4Tl/tk) was demonstratedby the method of DNA-PCR. Using a modified展开更多
文摘To apply gene therapy to breast carcinoma wepropose a model for in vitro gene transfer of the herpessimplex thymidine kinase (HSV-tk) gene using Stkvector-producing cells. In this study the HSV-tk gene wastransferred to mouse mammary carcinoma cell line 4Tlwith the method of infection. The HSV-tk gene in thegenome of transduced cells (4Tl/tk) was demonstratedby the method of DNA-PCR. Using a modified