To explore the influence and significance of the ischemia reperfusion on the hepatic neoplasm, the hepatic VX2 neoplasm model of rabbits was established under the guide of ultrasonography; and ischemia was caused by u...To explore the influence and significance of the ischemia reperfusion on the hepatic neoplasm, the hepatic VX2 neoplasm model of rabbits was established under the guide of ultrasonography; and ischemia was caused by using a non-traumatic vascular clamp to block the branches distributing in the left-middle lobe of the hepatic artery for 60 min, and subsequently the clamp was removed and the reperfusion injury of hepatic neoplasm occurred. At different time-points, the normal and hepatic neoplasm tissues of the animal models were taken out to detect the superoxide dismutase (SOD) and malondialdehyde (MDA) respectively.The results show that the products and injurious effects of oxygen free radical (OFR) of the neoplasm tissues are more serious than those of the normal hepatic tissues.展开更多
Background and Aims:The aim was to establish a liver venous deprivation(LVD)model in rats,compare hepatic hypertrophy between LVD and associated liver partition and portal vein ligation for staged hepatectomy(ALPPS),a...Background and Aims:The aim was to establish a liver venous deprivation(LVD)model in rats,compare hepatic hypertrophy between LVD and associated liver partition and portal vein ligation for staged hepatectomy(ALPPS),and explore the underlying mechanisms.Methods:The LVD or extended-LVD(e-LVD)group received portal vein ligation(PVL)combined with hepatic vein ligation(HVL).The ALPPS or eALPPS group received PVL plus parenchyma ligation.Liver regeneration was assessed by measuring the liver weight and performing pathological analysis.Liver functions and the sphingosine kinase 1(SPHK1)/sphingosine-1-phosphate(S1P)/sphingosine-1-phosphate receptor 1(S1PR1)pathway were also investigated.Results:All future liver remnants(FLRs)in the ALPPS,e-ALPPS,LVD,and e-LVD groups exhibited significant hypertrophy compared with the control group.The LVD and e-LVD procedures induced similar liver hypertrophy than that in the corresponding ALPPS groups.Furthermore,the LVD and e-LVD methods led to obvious cytolysis in the venous-deprived lobes as well as a noticeable increase in serum transaminase levels,while no necrosis was observed in the ALPPS and e-ALPPS groups.SPHK1/S1P/S1PR1 pathway were distinctly activated after operation,especially in congestive/ischemic livers.Conclusions:We describe the first rat model of LVD and e-LVD with simultaneously associated HVL and PVL.Compared with the ALPPS technique,the LVD or e-LVD procedure had a comparable overall effect on the hypertrophy response and a stronger effect on liver function.The SPHK1/S1P/S1PR1 pathway was involved in the LVD-or ALPPS-induced liver remodeling.展开更多
文摘To explore the influence and significance of the ischemia reperfusion on the hepatic neoplasm, the hepatic VX2 neoplasm model of rabbits was established under the guide of ultrasonography; and ischemia was caused by using a non-traumatic vascular clamp to block the branches distributing in the left-middle lobe of the hepatic artery for 60 min, and subsequently the clamp was removed and the reperfusion injury of hepatic neoplasm occurred. At different time-points, the normal and hepatic neoplasm tissues of the animal models were taken out to detect the superoxide dismutase (SOD) and malondialdehyde (MDA) respectively.The results show that the products and injurious effects of oxygen free radical (OFR) of the neoplasm tissues are more serious than those of the normal hepatic tissues.
基金supported by the Fundamental Research Funds for the Central Universities (NO.2042020kf0124)the National Natural Science Foundation of China (NO.82001940).
文摘Background and Aims:The aim was to establish a liver venous deprivation(LVD)model in rats,compare hepatic hypertrophy between LVD and associated liver partition and portal vein ligation for staged hepatectomy(ALPPS),and explore the underlying mechanisms.Methods:The LVD or extended-LVD(e-LVD)group received portal vein ligation(PVL)combined with hepatic vein ligation(HVL).The ALPPS or eALPPS group received PVL plus parenchyma ligation.Liver regeneration was assessed by measuring the liver weight and performing pathological analysis.Liver functions and the sphingosine kinase 1(SPHK1)/sphingosine-1-phosphate(S1P)/sphingosine-1-phosphate receptor 1(S1PR1)pathway were also investigated.Results:All future liver remnants(FLRs)in the ALPPS,e-ALPPS,LVD,and e-LVD groups exhibited significant hypertrophy compared with the control group.The LVD and e-LVD procedures induced similar liver hypertrophy than that in the corresponding ALPPS groups.Furthermore,the LVD and e-LVD methods led to obvious cytolysis in the venous-deprived lobes as well as a noticeable increase in serum transaminase levels,while no necrosis was observed in the ALPPS and e-ALPPS groups.SPHK1/S1P/S1PR1 pathway were distinctly activated after operation,especially in congestive/ischemic livers.Conclusions:We describe the first rat model of LVD and e-LVD with simultaneously associated HVL and PVL.Compared with the ALPPS technique,the LVD or e-LVD procedure had a comparable overall effect on the hypertrophy response and a stronger effect on liver function.The SPHK1/S1P/S1PR1 pathway was involved in the LVD-or ALPPS-induced liver remodeling.
