基于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯电化学免疫传感器检测Anti-IgG含量的研究

应用物理吸附法将羊抗人IgG抗原直接固定于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯修饰的金电极表面,制备电化学免疫传感器。采用循环伏安法和交流阻抗法对传感器进行表征,结果表明该传感器适合检测Anti-IgG浓度。同时探讨了缓冲液pH值、扫描速度、免疫反应温度、抗原与抗体配比对循环伏安峰电流的影响,结果表明在5-100mV/s扫速范围内,峰电流与扫速呈线性。孵育最优条件为25℃,h-IgG与Anti-IgG配比为1∶1。循环伏安法研究还表明Anti-IgG浓度在0.01-260μg/L范围内,线性关系良好,相关系数r^2=0.993,检出限（S/N=3）为0.006μg/L,据此建立了检测Anti-IgG浓度的新方法。

信号分子IRAK1/4在antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF中的作用探讨

目的:探讨IRAK1和IRAK4在antiβ-2GPI/β2GPI复合物诱导单核细胞株THP-1表达组织因子(TF)中的作用。方法:利用荧光定量PCR(Real-time PCR)、TF活性试剂盒分别检测THP-1细胞表达TF mRNA及TF活性;Western blot检测anti-β2GPI/β2GPI复合物诱导THP-1细胞表达IRAK1、磷酸化-IRAK1(p-IRAK1)、IRAK4情况;观察IRAK1/4抑制物是否干预anti-β2GPI/β2GPI复合物诱导THP-1表达TF。结果:Antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1细胞表达TF显著增加(P<0.05 vs control);Antiβ-2GPI/β2GPI复合物(100μg/ml)刺激THP-1细胞表达IRAK1、p-IRAK1、IRAK4(蛋白)显著升高(P<0.05 vscontrol);IRAK1/4抑制物(50μmol/L)能够阻断antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1表达TF及IRAK1磷酸化的效应。结论:antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF过程中,信号分子IRAK1/4被激活进而发挥重要作用。

Changes of plasma D(-) -lactate, diamine oxidase and endotoxin in patients with liver cirrhosis

BACKGROUND: Plasma D(-)-lactate and diamine oxidase (DAO) can reflect patients' intestinal mucosal condition. We evaluated the changes of plasma D (-)-lactate, DAO and endotoxin activities and their significance in patients with liver cirrhosis. METHODS: Fifty liver cirrhosis patients were enrolled into experimental group and 30 healthy people into control group. The plasma levels of D(-)-lactate, DAO and endo- toxin were detected spectrophotographically. RESULTS: The level of D(-)-lactate was significantly high- er in the experimental group than that in the control group (P<0.01). Significant differences of D (-)-lactate levels were observed in Child-Pugh subgroups of the experimen- tal group (P <0. 01). The level of DAO was significantly higher in the experimental group than that in the control group (P <0.01), but the level of DAO in Child-Pugh sub- group C was significantly lower than that in Child-Pugh subgroup B (P<0.01). The level of endotoxin was signifi- cantly increased in the experimental group except Child Pugh subgroup A (P<0.01). The plasma levels of D(-) lactate, DAO and endotoxin were positively correlated with each other (P<0.01). CONCLUSIONS: The data suggest that both plasma D(-) lactate and DAO activity are sensitive markers for early diagnosis of gut failure and endotoxemia in patients with liver cirrhosis. The impairment of intestinal barrier func- tion may be one of the critical reasons for deterioration of liver cirrhosis.

三维Anti de Sitter空间中Lorentzian曲面的S_t^1×S_s^1-值光锥Gauss映射的奇点分类

利用Arnol'd的Legendrian理论,对三维Anti de Sitter空间中Lorentzian曲面进行了研究.引入光维高度函数概念研究了三维Anti de Sitter空间Lorentzian曲面的S1t×S1s-值、光锥Gauss映射的奇点,进行了奇点分类,揭示了类光Causs-kronecker曲率之间的关系;并研究了Lorentzian曲面的一些基本几何性质.

Association of promoter polymorphism of the CD14 C (-159) T endotoxin receptor gene with chronic hepatitis B

AIM: To investigate whether single-nucleotide polymor- phisms in the promoter regions of endotoxin-responsive genes CD14 C (-159) T is associated with chronic hepatitis B. METHODS: We obtained genomic DNA from 80 patients with established diagnosis of chronic hepatitis B and 126 healthy subjects served as a control population. The CD 14 C (-159) T polymorphism was investigated using an allele specific PCR method. RESULTS: Twenty seven percent of chronic hepatitis B patients and 75% of controls were heterozygous for CT genotype. The difference between the chronic hepatitis B and control groups was statistically significant [P < 0.0001; Odds ratio (OR) = 2.887; 95% CI: 1.609-5.178]. Twenty four point six percent of chronic hepatitis B and patients 12.3% of the control group were heterozygous for TT genotype. The difference between groups was not statistically significant (P = 0.256; OR = 0.658; 95% CI: 0.319-1.358). Forty eight point four percent of chronic hepatitis B patients and 12.7% of control were homozy- gote for CC genotype (P < 0.004; OR = 0.416; 95% CI: 0.229-0.755). The frequency of allele C was 61.9% and allele T was 38.1% in hepatitis B patients group. The frequency of allele C was 55.2% and allele T was 44.8% for the control group (P = 0.179; OR = 1.319; 95% CI: 0.881-1.977). CONCLUSION: The TT heterozygous genotype was not a risk factor for chronic hepatitis B. CC homozygote genotype is protective for hepatitis B. Lack of heterozy- gosis of genotype CT is a risk factor for chronic hepatitis B. Alleles C or T were not risk factors for chronic hepatitis B. These findings show the role of a single-nucleotide polymorphism at CD14/-159 on the development ofchronic hepatitis B. Endotoxin susceptibility may play a role in the pathogenesis of chronic hepatitis B.

Anti-Endotoxic Effects of Syringic Acid of Radix Isatidis

The anti-endotoxic effect of syringic acid (SA) isolated from Radix Isatidis (Banlangen, BLG) was studied. SA was extracted and isolated from BLG and diluted into 1 % solution. The content of SA-pretreated endotoxin (ET) was quantitatively determined using Limulus test. The ability of fever induction of ET pretreated with SA was measured using endotoxin-induced fever test in rabbits. The LPS-induced death in mice pretreated with and without SA was compared. Results showed that after pretreatment with SA, 83. 16 % of ET was destroyed, the ET-induced fever in rabbits relieved markedly and the LPS-induced death rate in mice dropped from 68 % to 20 %. It was concluded that SA isolated from BLG had anti-endotoxic effects.

On the Construction of Deepening Anti - corruption Mechanism

It needs the foundation of system and the guarantee of organizational system for anti-corruption,but it is more necessary to build and form an effective anti-corruption mechanism,so that the anti-corruption can be really put into practice. Anti-corruption mechanism refers to a organic operation system of the interaction,interconnection and constraint between the constituent elements( parts) and elements of national anti-corruption,and as a system,anti-corruption mechanism should have the characteristics of system aticness,comprehensiveness,transparency,legalization,public participation,scientific dynam ic,and internationalism. The construction of deepening anti-corruption mechanism is the need for reconstructing the ruling legitimacy of the party and the governm ent. Adhering to the principle of treating both root causes and symptoms is necessary in the construction of anti-corruption m echanism,com bating and punishing corruption is an important part of anti-corruption,and the prevention and control of corruption is the basic project of anti-corruption. Therefore,the construction of prevention and control mechanism in the anti-corruption mechanism has a more far-reaching significance.

Anti-Bacterial and Flame Retardant for Cotton Fabric by One-Bath Finishing

A research on the process of cotton fabric flame-re-tarding,anti-bacterial finishing and one-bath finish-ing of anti-bacterial and flame-retarding is discussed.The flame retardant agent was phosphorous-contained,and the bacteriostatic finishing agent named SFR-1 wassynthesized.The flame retardancy of the fabric finishedcan meet the DOC FF3-71 Children Sleepwear Stan-dard.Its bacterial inhibiting capacity can meet and ex-ceed the requirements of similar products

Testing of Anti-stripping Property of Friction Spun Core Yarn

Friction spun core yarn has two components: filament core and staple fiber sheath. Under axial rubbing action, the failure mode of the core yarn is the stripping of the sheath from the core. This paper introduces a method to test the anti - stripping property of the core yarn. With a modified Universal Testing Machine, the stripping resistance of friction spun core yarn can be continuously measured. Some factors Influencing the measurements are discussed in detail. The testing results are compared with those from a Y731 Yarn Abrasion Tester and fur - ther confirmed by weaving practice.

抗类脂A治疗梗阻性黄疸病人血液IL-6、IL-8水平的影响

目的：观察梗阻性黄疸病人术前抗内毒素治疗对内毒素血症抑制及ＩＬ－６、ＩＬ－８的影响。方法：梗阻性黄疸病人分３组在术前分别给予一般手术治疗（ＯＪ组），口服胆盐（ＯＪＴ１组）和静脉注射抗内毒素抗体（ＯＪＴ２组）观察血浆内毒素（ＥＴ）、ＩＬ－６及ＩＬ－８含量变化。结果：３组术前ＥＴ、ＩＬ－６和ＩＬ－８水平差异不显著。ＯＪＴ１组和ＯＪＴ２组用药后血浆ＥＴ水平均显著下降，明显低于ＯＪ组（Ｐ＜０．０１），术后进一步降低。ＩＬ－６在术后１４ｄ显著降低（Ｐ＜０．０１），ＩＬ－８在术后７ｄ降低（Ｐ＜０．０５）。结论：术前应用胆盐和抗内毒素抗体可有效降低梗阻性黄疸病人围手术期血浆ＥＴ、ＩＬ－６及ＩＬ－８水平。

家电用HIPS/nano-TiO_2多功能复合材料的性能

研究了家电用HIPS/nano TiO2 多功能复合塑料的加工流变性、母料分散性、吸光行为、抗菌、分解内毒素、分解异味气体及自清洁特性。结果表明 ,使用质量分数在0 5 %～ 2 0 %的nano TiO2 的复合材料加工流动性优于HIPS ;经EVA蜡改性的HIPS/nano TiO2 母料可得到良好分散效果 ;HIPS/nano TiO2 制品表现出良好的紫外吸收能力 ,nano TiO2 质量分数在 1 .0 %左右时 ,制品具有较好的抗菌、分解内毒素效果。

板蓝根对内毒素致HL-60细胞释放TNF-α和IL-8的影响

目的:探讨板蓝根抗内毒素活性部位对内毒素(LPS)致HL-60细胞释放肿瘤坏死因子(TNF-α)和白细胞介素8(IL-8)的影响、方法:MTT法确定最适细胞浓度和供试液浓度;ELISA法定量检测LPS预刺激、板蓝根预刺激、LPS与板蓝根同时刺激等三种给药方式作用后TNF-α和IL-8的浓度,观察三种给药方式对内毒素诱导TNF-α、IL-8释放的抑制强度。结果:细胞密度在0.25～2×10~5 cell·mL^(-1)范围时,吸光度与细胞数呈良好的线性关系;供试液无毒界限为7.812mg·mL^(-1);三种给药方式均能显著抑制LPS诱导HL-60细胞释放TNF-α仅和IL-8,其中供试液与LPS共育时抑制作用最强。结论:板蓝根可能通过抑制炎性因子过度释放发挥其抗内毒素作用。

CD14-159C/T基因多态性对CD14表达及炎症介质平衡的影响

目的:探讨CD14基因启动子-159C/T基因多态性对全血培养CD14表达及促炎/抗炎细胞因子平衡的影响。方法:采集118例健康献血员全血标本,运用聚合酶链反应(PCR)及限制性内切酶HaeIII对PCR产物的消化作用检测CD14基因多态性。采用全血细胞培养模型检测内毒素(LPS)刺激前后CD14mRNA表达、可溶性CD14(sCD14)浓度的变化以及LPS对促炎细胞因子TNF-α、IL-6及抗炎细胞因子IL-10的诱生水平的影响。结果:LPS刺激后,基因型TT与TC白细胞中CD14mRNA的表达及上清sCD14浓度均显著高于CC纯合子(P<0.05或0.01)。TT纯合子促炎细胞因子TNF-α的诱生水平明显高于基因型TC、CC,而抗炎细胞因子IL-10的诱生水平明显低于后两者(P<0.05或P<0.01)。T等位基因携带者的IL-6诱生水平则均显著高于CC纯合子(P<0.01)。结论:内毒素受体CD14-159C/T基因多态性对全血培养CD14的表达及释放产生明显影响,并与内毒素刺激后促炎/抗炎细胞因子平衡密切相关。

连续性静脉-静脉血液滤过治疗全身炎症反应综合征的作用机制

目的:探讨连续性静脉-静脉血液滤过技术(CVVH)对全身炎症反应综合征(SIRS)的治疗机制。方法:SIRS患者80例随机分为CVVH治疗组和对照组,分别实行CVVH治疗和常规治疗。监测治疗前后SIRS激活物质内毒素(ET)及与SIRS病程发展密切相关炎症因子及抗炎因子水平;对两组临床指标和预后情况进行分析。结果:(1)CVVH对SIRS患者主要致病因素-内毒素及相关因子降钙素原的清除作用较为显著,内毒素6h时即已清除40%,24h清除率达到80%以上。(2)CVVH组患者血清各炎症因子水平在6小时明显下降,与对照组比较差异有统计学意义(均P<0.01),12h、24h及48h各炎症因子水平持续下降。(3)CVVH组患者体温、心率、呼吸频率和外周血白细胞计数4指标总体改善率达92.50%,对照组的55.00%,两组比较差异有统计学意义(χ2=13.60,P<0.01)。(4)CVVH组MODS的发生率明显低于对照组(χ2=17.64,P<0.01)。发生MODS患者行序贯器官衰竭评分(SOFA评分),CVVH组SOFA评分明显低于对照组(P<0.05)。结论 :有效清除患者体内与SIRS激活、级联放大效应以及免疫失衡等环节相关的物质是CVVH治疗SIRS的作用机制,CVVH可以有效阻断SIRS向MODS发展的进程。

EFFECTS OF ACUPUNCTURE ON THE CONTENTS OF ENKEPHALINS IN DIFFERENT BRAIN REGIONS AND CORTICOSTERONE IN PLASMA OF RATS WITH ENDOTOXIN SHOCK

In this experiment,we have observed the effects of acupuncture on the contents ofenkephalins in different brain regions and corticosterone in plasma of rats with endotoxin shock.Theresults showed that after acupuncture the endogenous enkephalins were significantly decreased(P【0.05)in the midbrain and brain stem of endotoxin shock rats,suggesting the anti-shock of acupuncture might be related to the enkephalins in the midbrain and brain stem.In addition,there was a rising trend in plasma corticosterone of the rats with toxic shock.

<i>In Vivo</i>and <i>in Vitro</i>Evaluation of Anti Diabetic and Insulin Secretagogue Activities of <i>Capparis zeylanica</i>

Since ancient times, traditional medicines have been in the usage for the treatment of Diabetes mellitus. An edible fruit from traditional medicinal plant Capparis zeylanica (CZ) was studied for its anti diabetic, insulin secretagogue activities and mechanisms involved in it. In Streptozotocin induced diabetes rats, oral administration of Capparis zeylanica methanolic extract (CZME) (200 mg/kg body weight) for 28 days showed a significant reduction in blood glucose levels by 35.53% and enhanced circulating insulin levels by 81.82% than the diabetic control rats. The insulin secretagogue activity mechanisms of the extract were evaluated by using mouse insulinoma beta cell line (MIN6-β). The extract stimulated insulin release in dependent manner of glucose concentration (3 - 16.7 mM) and extract dose (5 - 500 μg/mL). The insulin releasing effect of the extract was significantly enhanced by 3-isobutyl-1-methyl xanthine, glibenclamide, elevated extracellular calcium and K+ depolarized media. This insulin release was significantly reduced in calcium blocking conditions (by nifedipine and EGTA), in the presence of potassium channel opener (diazoxide). Hence, anti diabetic activity of CZME might be a result of its stimulatory effect on insulin release from pancreatic beta cells via KATP channel dependent and independent ways. These results indicate that CZ fruits have the potential to use in diabetes therapy.

Exact Traveling Wave Solutions of Nano-Ionic Solitons and Nano-Ionic Current of MTs Using the exp(-φ (ξ ))-Expansion Method

In this work, the exp(-φ (ξ )) -expansion method is used for the first time to investigate the exact traveling wave solutions involving parameters of nonlinear evolution equations. When these parameters are taken to be special values, the solitary wave solutions are derived from the exact traveling wave solutions. The validity and reliability of the method are tested by its applications to Nano-ionic solitons wave’s propagation along microtubules in living cells and Nano-ionic currents of MTs which play an important role in biology.

运动与肠道菌健康效益的关系

背景:运动已成为当下促进人体健康不可或缺的重要方式之一。近年肠道组织中的菌群作为预防和改善人体疾病的重要靶点,其对人体健康的重要性已逐渐得到人们的认识。当前研究已证明,运动可以介入肠道菌群对其组成、结构、功能及多样性进行有效调控,从而影响机体组织健康状况。目的:文章首先对运动、肠道菌群以及机体健康3者的相关性进行系统分析,旨在进一步探究运动介入肠道菌群对机体组织健康产生影响的可能性机制;其次不同运动强度下菌群变化对机体健康影响状况进行分析,以期对相关疾病的改善和后续研究提供思路和途径。方法:检索Web of Science、Elsevier、Springer、PubMed、ProQuest、中国知网、万方数据、维普中文期刊服务平台及中国台湾学术文献数据库,检索与运动训练、肠道菌群、能量代谢、抗炎反应和氧化应激等相关的中英文文献,文献发表时间截止至2022年2月,根据研究目的确立相应的入组标准,最终筛选出97篇文献进行论述。结果与结论:①运动可以通过调节肠道菌群促进短链脂肪酸分泌促进抗炎因子白细胞介素10的增加,降低脂多糖诱导产生的相关炎症因子,从而提高机体组织抗炎能力,促进宿主健康。②运动介入肠道菌群不仅可以促进短链脂肪酸的产生也可以提高胆汁酸分泌来激活人体器官中的核激素法尼醇X受体和G蛋白偶联受体,从而调节宿主机体脂质、葡萄糖和能量代谢,促进机体各组织能量代谢吸收与稳定。③运动介入肠道菌群改变乳杆菌与双歧杆菌丰度调控抗氧化酶,如超氧化物歧化酶、过氧化氢酶及非酶系统谷胱甘肽的活性,从而调控或消除氧化应激,预防病原体入侵,改善肌肉组织损伤。④另外,有研究还发现不同强度运动对肠道菌群的影响有明显差异,从而对机体组织健康也产生不同影响,低-中强度运动介入肠道菌群可以改善肠道有益菌丰度,促进肠道稳态,对机体健康产生有益影响,而高强度运动介入肠道菌群对人体健康的影响存在较大争议。⑤目前,运动介入肠道菌群促进机体健康的相关机制尚不明朗,亟待深入研究,且对不同运动强度、运动时间、运动模式、运动对象等相关特定因素影响下肠道菌群对机体健康作用的具体研究较少,值得学者们进一步关注。

板蓝根F_(022)部位抗内毒素活性研究

目的 :研究板蓝根F0 2 2 部位抗内毒素活性并初步探讨其作用机理。方法 :ELISA法检测内毒素刺激鼠腹腔巨噬细胞分泌肿瘤坏死因子α(TNF α)、白介素 6 (IL 6 )水平 ;内毒素致兔发热实验 ;内毒素致BCG增敏小鼠死亡实验。结果 :F0 2 2 与LPS同时或F0 2 2 提前 1h处理巨噬细胞 ,能显著性抑制上清中TNF α和IL 6水平。先给予F0 2 2 ,能拮抗内毒素所致家兔发热反应 ,显著延长BCG增敏小鼠受LPS攻击后的存活时间 ,并呈剂量依赖性。结论 :以上结果提示F0 2 2 对内毒素拮抗作用发生在LPS激活机体免疫系统之前 ,该过程F0 2 2

抗菌药物诱导内毒素的产生及抗内毒素药物的研究进展

内毒素是革兰阴性菌的主要致病成分,抗菌药物在杀/抑菌的同时会诱导内毒素的产生,增加了疾病的治疗难度。体外实验、动物实验证实,抗菌药物诱导内毒素的产生与多种因素有关,提示在临床选用抗菌药物时应对药敏实验结果及其诱导内毒素释放的特性进行综合考虑。抗内毒素药物的研究近年取得了一些成效,其中中药制剂也显示了很好的抗内毒素作用,相信这类药物会有广阔的发展前景。