Objective:To screen the anti-fungal effects and find out the active metabolites from sponge, Sigmadocia carnosa(S.carnosa) against four dermatophytic fungi.Methods:The methanol,ethyl acetate and acetone extract of mar...Objective:To screen the anti-fungal effects and find out the active metabolites from sponge, Sigmadocia carnosa(S.carnosa) against four dermatophytic fungi.Methods:The methanol,ethyl acetate and acetone extract of marine sponge,S.carnosa was examined against Trichaphyton mentagrophytes(T.mentagrophytes),Trichophyton rubrum(T.rubrum),Epidermophyton floccosum(E.floccosum) and Microsporum gypseum(M.gypseum) and qualitative analysed to find out the active molecules.Results:The methanol extract of sponge was expressed significant activity than ethyl acetate and acetone.The minimum inhibitory concentration(MIC) of methanol extract of sponge that resulted in complete growth inhibition of T.mentagrophytes,T.rubrum, E.floccosum and M.gypseum were found to 125,250,250 and 250 μg/mL respectively.But, 100%inhibition of fungal spore germination was observed in T.mentagrophytes at 500 μg/mL concentration followed by T.rubrum,E.floccosum and At.gypseum at 1000 μg/mL concentration. Other two extracts showed weak anti spore germination activity against the tested dermatophyte fungi.Methanol extracts showed presence of terpenoids,steroids,alkaloids,saponins and glycosides.Conclusion:Based on the literature,this is the first study which has conducted to inhibit the growth and spore germination of dermatophytic fungi with S.carnosa.Further research also needs to purify and characterize the secondary metabolites from the sponge,5.carnosa for the valuable source of novel substances for future drug discovery.展开更多
Objective:To isolate and characterize the bioactive secondary metabolite from Callyspongia spp.associated fungi.Methods:In vitro antibacterial screening of fungi associated with Callyspongia species,collected from sou...Objective:To isolate and characterize the bioactive secondary metabolite from Callyspongia spp.associated fungi.Methods:In vitro antibacterial screening of fungi associated with Callyspongia species,collected from south east coast of India,against selected clinical isolates of bacteria were conducted in this study.The extracts showing good antimicrobial activity were subjected to further analysis to identify the active constituents sponge associated fungi(both biomass and filtrate) with five different solvents.The compound responsible for bioactivity was characterized using Fouvier-transform infrared(FT-IR) and gas chromatography-mass spectrometry(GC-MS) instrumental analysis to identify the functional group and compound.The molecular characterization of the elite fungal strains were done by isolating their genomic DNA and amplify the internal transcribed spacer(ITS) region of 5.8srRNA using specific ITS primer. The novelty of the strain was proved by BlastN analysis against non-redundant(NR) database and hence was submitted to GenBank.Results:Active compound was Desmethylnomifensine confirmed by GC-MS and the potent fungi was Aspergillus flavus GU815344.Conclusions: The isolate exhibits a marked antagonistic activity against potential bacterial pathogens thus illuminating the advanced researches in this decade to focus on clinical pharmacology to identify novel therapeutic targets.The present study depicts a promising scenario to focus on Aspergillus flavus derived compounds which can be easily scaled up for large biomass production and stable formulation as a drug.展开更多
基金Funded by University Grants Commission(UGC),New Delhi.(grant No.U.G.C.No.33-384/2007(SR)
文摘Objective:To screen the anti-fungal effects and find out the active metabolites from sponge, Sigmadocia carnosa(S.carnosa) against four dermatophytic fungi.Methods:The methanol,ethyl acetate and acetone extract of marine sponge,S.carnosa was examined against Trichaphyton mentagrophytes(T.mentagrophytes),Trichophyton rubrum(T.rubrum),Epidermophyton floccosum(E.floccosum) and Microsporum gypseum(M.gypseum) and qualitative analysed to find out the active molecules.Results:The methanol extract of sponge was expressed significant activity than ethyl acetate and acetone.The minimum inhibitory concentration(MIC) of methanol extract of sponge that resulted in complete growth inhibition of T.mentagrophytes,T.rubrum, E.floccosum and M.gypseum were found to 125,250,250 and 250 μg/mL respectively.But, 100%inhibition of fungal spore germination was observed in T.mentagrophytes at 500 μg/mL concentration followed by T.rubrum,E.floccosum and At.gypseum at 1000 μg/mL concentration. Other two extracts showed weak anti spore germination activity against the tested dermatophyte fungi.Methanol extracts showed presence of terpenoids,steroids,alkaloids,saponins and glycosides.Conclusion:Based on the literature,this is the first study which has conducted to inhibit the growth and spore germination of dermatophytic fungi with S.carnosa.Further research also needs to purify and characterize the secondary metabolites from the sponge,5.carnosa for the valuable source of novel substances for future drug discovery.
文摘Objective:To isolate and characterize the bioactive secondary metabolite from Callyspongia spp.associated fungi.Methods:In vitro antibacterial screening of fungi associated with Callyspongia species,collected from south east coast of India,against selected clinical isolates of bacteria were conducted in this study.The extracts showing good antimicrobial activity were subjected to further analysis to identify the active constituents sponge associated fungi(both biomass and filtrate) with five different solvents.The compound responsible for bioactivity was characterized using Fouvier-transform infrared(FT-IR) and gas chromatography-mass spectrometry(GC-MS) instrumental analysis to identify the functional group and compound.The molecular characterization of the elite fungal strains were done by isolating their genomic DNA and amplify the internal transcribed spacer(ITS) region of 5.8srRNA using specific ITS primer. The novelty of the strain was proved by BlastN analysis against non-redundant(NR) database and hence was submitted to GenBank.Results:Active compound was Desmethylnomifensine confirmed by GC-MS and the potent fungi was Aspergillus flavus GU815344.Conclusions: The isolate exhibits a marked antagonistic activity against potential bacterial pathogens thus illuminating the advanced researches in this decade to focus on clinical pharmacology to identify novel therapeutic targets.The present study depicts a promising scenario to focus on Aspergillus flavus derived compounds which can be easily scaled up for large biomass production and stable formulation as a drug.
