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Anti-Fibrotic Effects of Calotropis procera (Ait.) R.Br Roots Barks against Diethylnitrosamine-Induced Hepatic Fibrosis in Rats
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作者 Ernest Nogma Sombié Tata Kadiatou Traoré +5 位作者 Abdoul-Nassiré Derra Jotham Yhi-Pênê N’do Wendkouni L. M. E. Belem-Kabré Noufou Ouédraogo Adama Hilou André Tibiri 《Journal of Biosciences and Medicines》 2023年第4期332-349,共18页
Background: Liver diseases including chronic hepatitis, steatosis, fibrosis, cirrhosis and liver cancer are now a public health problem. In 2002, cirrhosis accounted for 27.63% of hepatobiliary diseases in Burkina Fas... Background: Liver diseases including chronic hepatitis, steatosis, fibrosis, cirrhosis and liver cancer are now a public health problem. In 2002, cirrhosis accounted for 27.63% of hepatobiliary diseases in Burkina Faso. In Africa and more particularly in Burkina Faso, the majority of the population (about 80%) uses medicinal plants for their primary health care. Calotropis procera (Ait.) R.Br (Apocynaceae) is a medicinal plant used in Burkina Faso in the treatment of liver problems. This work aims to evaluate the anti-fibrotic properties of Calotropis procera roots barks. Methods: The anti-fibrotic activity of the ethanolic extract of Calotropis procera roots barks was evaluated using diethylnitrosamine (DEN) to induce liver fibrosis in male Wistar rats. Serum biomarkers, alanine transaminase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP), Total protein, Albumin, Υ-Glutamyl transferase (GGT) were evaluated and the activities of antioxidant enzymes (Superoxide dismutase and catalase) as well as the level of malonedialdehyde (MDA) and that of nitric oxide (NO) were determined in the liver homogenate. Results: The treatment of rats suffering from hepatic fibrosis with the ethanolic extract leads to a significant restoration of the biomarkers of the hepatic function in particular, AST, ALP, GGT, Albumin. The extract also causes a reduction in oxidative stress in the liver through a significant increase in the activity rate of the antioxidant enzymes Superoxide dismutase (SOD) and catalase accompanied by a significant drop in the rate of MDA and NO suggesting the anti-oxidant effect of extract. Conclusion: The results of the study show that the ethanolic extract of the roots barks of Calotropis procera has anti-fibrotic properties. 展开更多
关键词 hepatic fibrosis Calotropis procera anti-Fibrotic Properties Burkina Faso
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Assessment of Liver Fibrosis in HBsAg-Negative and Anti HBc Positive Patients
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作者 Kounpiélimé Sosthène Somda Aboubacar Coulibaly +4 位作者 Oyétoundé Taofick Amanda Sandrine Marie Odile Soudre Lawagoulé Joseph Emile Ky Christiane Bere Arsène Roger Sombie 《Open Journal of Gastroenterology》 CAS 2024年第10期331-339,共9页
Background: Surface antigen (HBsAg) is the mean marker of hepatitis B virus infection. During the course of the infection, some patients lose the HBsAg and only the presence of anti-HBc antibody indicates previous con... Background: Surface antigen (HBsAg) is the mean marker of hepatitis B virus infection. During the course of the infection, some patients lose the HBsAg and only the presence of anti-HBc antibody indicates previous contact with the virus. Among these patients, some have detectable viral load (occult infection) but most without viral replication. There is no guideline regarding these patients. The aim of this study was to assess hepatic fibrosis in patients with only the hepatitis B virus contact marker “total anti-HBc”. Patients and methods: it was a descriptive and analytical cross-sectional study, conducted in three private hospitals from January to August 2022. Were included HBsAg-negative and HBc-positive patients, consulting in Gastroenterology departments. Noninvasive methods (APRI, FIB-4 and FIBROSCAN) were used to evaluate liver stiffness because of their easy accessibility and low-cost. The hepatic fibrosis was considered significant when the score determined by APRI, FIB-4 and FIBROSCAN® tests was respectively greater than 1.5;2.67 and 8 kPa corresponding to fibrosis level 2 (F2). Results: A total of 63 HBsAg-negative/total HBcAg-positive patients were included. The mean age was 49.9 ± 13.4 years. The male/female sex ratio was 1.78. Of the 63 patients, 19 had significant liver fibrosis (30.1%) among which 9 patients had HCC. The FIB-4 score outperformed the APRI score in assessing liver fibrosis, with a sensitivity of 84.2%, a specificity of 100% and a negative predictive value of 93.6%. In univariate analysis, there was a significant association between the occurrence of significant liver fibrosis and age over 40 years, dyslipidaemia, obesity, alcohol consumption, smoking, herbal medicine, negative anti-HBs immunological status and detectable viral load. Conclusion: Our study revealed a high prevalence of significant to severe hepatic fibrosis in anti-HBc positive patients. In most of the cases, the fibrosis was severe. Progression to HCC has also been possible. There is no consensus on the follow-up strategy for those patients. However, screening for hepatic fibrosis using noninvasive methods should be recommended for patients aged over 40 years, alcohol or herbal medicine users, patients with metabolic syndrome or occult hepatitis B. In HBsAg-negative/anti-HBc-positive patients, liver stiffness should be evaluated and if it is greater than F2, HCC screening should be started. 展开更多
关键词 anti HBc Positives Liver fibrosis Sub-Saharan hepatitis B Virus
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Angiotensin-converting enzyme 2 alleviates liver fibrosis through the renin-angiotensin system 被引量:3
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作者 Bai-Wei Zhao Ying-Jia Chen +2 位作者 Ruo-Peng Zhang Yong-Ming Chen Bo-Wen Huang 《World Journal of Gastroenterology》 SCIE CAS 2024年第6期607-609,共3页
The present letter to the editor is related to the study titled‘Angiotensin-converting enzyme 2 improves liver fibrosis in mice by regulating autophagy of hepatic stellate cells’.Angiotensin-converting enzyme 2 can ... The present letter to the editor is related to the study titled‘Angiotensin-converting enzyme 2 improves liver fibrosis in mice by regulating autophagy of hepatic stellate cells’.Angiotensin-converting enzyme 2 can alleviate liver fibrosis by regulating autophagy of hepatic stellate cells and affecting the renin-angiotensin system. 展开更多
关键词 Angiotensin-converting enzyme 2 hepatic stellate cells Liver fibrosis Angiotensin II Angiotensin 1-7 Renin-angiotensin system
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Effects of Aqueous Extract of Plumbago zeylanica L. in the Reversal of DMN-induced Hepatic Fibrosis in Rat 被引量:3
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作者 Yue PENG Xuelin DUAN +3 位作者 Tiejian ZHAO Yanfei WEI Guiyu LI Lulu LIU 《Agricultural Biotechnology》 CAS 2017年第3期64-70,共7页
[ Objectives ] This study was conducted to evaluate the action of the aqueous extract of a medicinal plant Plumbago zeylanica L. in the reversal of dime- thylnitrosamine (DMN) -induced hepatic fibrosis in rats, and ... [ Objectives ] This study was conducted to evaluate the action of the aqueous extract of a medicinal plant Plumbago zeylanica L. in the reversal of dime- thylnitrosamine (DMN) -induced hepatic fibrosis in rats, and to provide a scientific basis for the utilization of P. zeylanica in treatment of hepatic fibrosis. [Meth- ods ] Hepatic fibrosis in rats was induced by intraperitoneal injection of DMN. The rats were then given the aqueous extract of P. zeylanica at high, medium or low concentration by garage for five weeks. Serum level of alanine aminotransferase (ALT) was determined by lactate dehydrogenase (LDH)-release assay, and serum level of aspartate transaminase (AST) was measured by UV-malate dehydrogenase (MDH) assay. Serum levels of total bilirubin (TBIL), direct bilirubin (DBIL) and indirect bilirubin (1BIL) were measured by vanadate oxidation assay. Four indices of hepatic fibrosis (hyaluronic acid, laminin, procollagen type III and colla- gen type IV) were determined by radioimmunoassay (RIA) assay. Morphological damage of liver tissue was observed by hematoxylin-eosin staining (H&E stai- ning). Immunohistochemical staining was performed to determine the location and area of deposited collagen type I, collagen type III and ct-smeoth muscle actin (a-SMA) in liver tissue. [ Results] Compared with the negative control (rats with diseased fiver and untreated with P. zeylanica aqueous extract), the serum lev- els of ALT, AST, TBIL, DBIL and IBIL were significantly decreased by P. zeylanica aqueous extract; the levels of the four serum indices of hepatic fibrosis were also obviously reduced. H&E staining showed that hepatic fibrosis in rats was obviously inhibited or even reversed by P. zeylanica aqueous extract. Immunohisto- chemical staining proved that the aqueous extract of P. zeylanica significantly reduced the area and coloration of collagen type I, collagen type III and ct-SMA in rat liver. [ Conclusions] The aqueous extract of P. zeylanica has a definite effect in reversal of DMN-indueed hepatic fibrosis in rat by promoting the recovery of liver function, reversal of histopathological changes and reducing fibrotic collagen. 展开更多
关键词 Plumbago zeylanica L. Aqueous extract Dimethylnitrosamine (DMN) anti-hepatic fibrosis
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Renin-angiotensin system in the pathogenesis of liver fibrosis 被引量:37
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作者 Regina Maria Pereira Robson Augusto Souza dos Santos +2 位作者 Filipi Leles da Costa Dias Mauro Martins Teixeira Ana Cristina Simoes e Silva 《World Journal of Gastroenterology》 SCIE CAS CSCD 2009年第21期2579-2586,共8页
Hepatic fibrosis is considered a common response to many chronic hepatic injuries. It is a multifunctional process that involves several cell types, cytokines, chemokines and growth factors leading to a disruption of ... Hepatic fibrosis is considered a common response to many chronic hepatic injuries. It is a multifunctional process that involves several cell types, cytokines, chemokines and growth factors leading to a disruption of homeostatic mechanisms that maintain the liver ecosystem. In spite of many studies regarding the development of fibrosis, the understanding of the pathogenesis remains obscure. The hepatic tissue remodeling process is highly complex, resulting from the balance between collagen degradation and synthesis. Among the many mediators that take part in this process, the components of the Renin angiotensin system (RAS) have progressively assumed an important role. Angiotensin (Ang) II acts as a profibrotic mediator and Ang-(1-7), the newly recognized RAS component, appears to exert a counter-regulatory role in liver tissue. We briefly review the liver fibrosis process and current aspects of the RAS. This review also aims to discuss some experimental evidence regarding the participation of RAS mediators in the pathogenesis of liver fibrosis, focusing on the putative role of the ACE2-Ang-(1-7)- Mas receptor axis. 展开更多
关键词 hepatic fibrosis Renin angiotensin system Angiotensin II Angiotensin-(1-7) Receptor Mas Angiotensin converting enzyme 2
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Inflammation and fibrosis in chronic liver diseases including nonalcoholic fatty liver disease and hepatitis C 被引量:23
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作者 Sudeep Tanwar Freya Rhodes +2 位作者 Ankur Srivastava Paul M Trembling William M Rosenberg 《World Journal of Gastroenterology》 SCIE CAS 2020年第2期109-133,共25页
At present chronic liver disease(CLD),the third commonest cause of premature death in the United Kingdom is detected late,when interventions are ineffective,resulting in considerable morbidity and mortality.Injury to ... At present chronic liver disease(CLD),the third commonest cause of premature death in the United Kingdom is detected late,when interventions are ineffective,resulting in considerable morbidity and mortality.Injury to the liver,the largest solid organ in the body,leads to a cascade of inflammatory events.Chronic inflammation leads to the activation of hepatic stellate cells that undergo transdifferentiation to become myofibroblasts,the main extra-cellular matrix producing cells in the liver;over time increased extra-cellular matrix production results in the formation of liver fibrosis.Although fibrogenesis may be viewed as having evolved as a“wound healing”process that preserves tissue integrity,sustained chronic fibrosis can become pathogenic culminating in CLD,cirrhosis and its associated complications.As the reference standard for detecting liver fibrosis,liver biopsy,is invasive and has an associated morbidity,the diagnostic assessment of CLD by non-invasive testing is attractive.Accordingly,in this review the mechanisms by which liver inflammation and fibrosis develop in chronic liver diseases are explored to identify appropriate and meaningful diagnostic targets for clinical practice.Due to differing disease prevalence and treatment efficacy,disease specific diagnostic targets are required to optimally manage individual CLDs such as non-alcoholic fatty liver disease and chronic hepatitis C infection.To facilitate this,a review of the pathogenesis of both conditions is also conducted.Finally,the evidence for hepatic fibrosis regression and the mechanisms by which this occurs are discussed,including the current use of antifibrotic therapy. 展开更多
关键词 Liver inflammation fibrosis CIRRHOSIS Non-alcoholic fatty liver disease Chronic hepatitis C Chronic liver disease anti-fibrotic BIOMARKER
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Anti-miR34c树状纳米颗粒对裸鼠肝纤维化的治疗作用 被引量:1
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作者 张欢欢 方杰 +3 位作者 俞文英 杨扬 余陈欢 应华忠 《中国实验动物学报》 CAS CSCD 北大核心 2018年第5期587-591,共5页
目的探索anti-miR34c树状纳米颗粒对裸鼠肝纤维化的治疗效果。方法将清洁级(20±2)g裸鼠随机分为正常组、模型组、anti-miR34c树状纳米颗粒治疗组,每组5只,腹腔注射四氯化碳诱导肝纤维化模型。HE染色观察肝组织病理变化,Masson染色... 目的探索anti-miR34c树状纳米颗粒对裸鼠肝纤维化的治疗效果。方法将清洁级(20±2)g裸鼠随机分为正常组、模型组、anti-miR34c树状纳米颗粒治疗组,每组5只,腹腔注射四氯化碳诱导肝纤维化模型。HE染色观察肝组织病理变化,Masson染色评价胶原沉积情况,ELISA检测血清生化指标,免疫组化检测TGF-β和α-SAM在肝组织中的表达变化。结果 anti-miR34c树状纳米颗粒治疗组的肝组织炎症细胞浸润减少,胶原沉积降低,肝纤维化指标TGFβ和α-SAM表达降低,血清生化指标好转。结论 anti-miR34c的树状纳米颗粒能够有效改善裸鼠肝纤维化肝损伤,有望成为治疗肝纤维化的新策略。 展开更多
关键词 肝纤维化 anti-miR34c 树状纳米颗粒
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Role of ethanol in the regulation of hepatic stellate cell function 被引量:16
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作者 Jian-Hua Wang Robert G Batey Jacob George 《World Journal of Gastroenterology》 SCIE CAS CSCD 2006年第43期6926-6932,共7页
Evidence has accumulated to suggest an important role of ethanol and/or its metabolites in the pathogenesis of alcohol-related liver disease. In this review, the fibrogenic effects of ethanol and its metabolites on he... Evidence has accumulated to suggest an important role of ethanol and/or its metabolites in the pathogenesis of alcohol-related liver disease. In this review, the fibrogenic effects of ethanol and its metabolites on hepatic stellate cells (HSCs) are discussed. In brief, ethanol interferes with retinoid metabolism and its signaling, induces the release of fibrogenic cytokines such as transforming growth factor β-1 (TGFβ-1) from HSCs, up-regulates the gene expression of collagen I and enhances type I collagen protein production by HSCs. Ethanol further perpetuates an activated HSC phenotype through extracellular matrix remodeling. The underlying pathophysiologic mechanisms by which ethanol exerts these pro-fibrogenic effects on HSCs are reviewed. 展开更多
关键词 ETHANOL ACETALDEHYDE hepatic stellate cells liver fibrosis Type I collagen gene Transcription factors Transforming growth factor β-1
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Serum levels of angiotensin converting enzyme as a biomarker of liver fibrosis 被引量:3
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作者 Aline Silva Miranda Ana Cristina Sim?es e Silva 《World Journal of Gastroenterology》 SCIE CAS 2017年第48期8439-8442,共4页
The renin angiotensin system(RAS) is classically conceived as a circulating hormonal system involved in blood pressure control and hydroelectrolyte balance. The discovery that RAS components are locally expressed in a... The renin angiotensin system(RAS) is classically conceived as a circulating hormonal system involved in blood pressure control and hydroelectrolyte balance. The discovery that RAS components are locally expressed in a wide range of organs and tissues,including the liver,pointed to a role for this system in the pathogenesis of several conditions including hepatic fibrosis and cirrhosis. It has been widely reported that the classical RAS axis composed by the angiotensin converting enzyme(ACE)-angiotensin(Ang) Ⅱ-Ang type 1(AT1) receptor mediates pro-inflammatory,pro-thrombotic,and pro-fibrotic processes. On the other hand,the alternative axis comprising ACE2-Ang-(1-7)-Mas receptor seems to play a protective role by frequently opposing Ang Ⅱ action. Chronic hepatitis B(CHB) is one of the leading causes of liver fibrosis,accounting for the death of nearly one million people worldwide. Liver fibrosis is a key factor to determine therapeutic interventions for patients with CHB. However,the establishment of non-invasive and accurate methods to detect reversible stages of liver fibrosis is still a challenge. In an elegant study published in the 36 th issue of the World Journal of Gastroenterology,Noguchi et al showed the predictive value of serum ACE levels in detecting not only advanced stages of liver fibrosis but also initial and intermediate fibrotic stages. The serum levels of ACE might represent an accurate,non-invasive,widely available,and easy method to evaluate fibrosis related to CHB. Moreover,therapies involving the inhibition of the classical RAS axis components might be promising in the control of CHB-related liver fibrosis. 展开更多
关键词 RENIN ANGIOTENSIN system ANGIOTENSIN CONVERTING enzyme ANGIOTENSIN Angiotensin-(1-7) Chronic hepatitis B hepatic CIRRHOSIS Liver fibrosis
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Comparison on Efficacy between Astragalus-Polygonum Anti-Fibrosis Decoction and Jinshuibao (金水宝) Capsule in Treating Liver Fibrosis of Chronic Hepatitis B 被引量:1
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作者 陈汉诚 翁伦华 《Chinese Journal of Integrative Medicine》 SCIE CAS 2001年第1期26-29,共4页
Objective: To observe the efficacy of Astragalus Polygonum Anti Fibrosis decoction (APAFD)and Jinshuibao capsule (JSBC) in treating liver fibrosis of chronic hepatitis B. Methods: Ninety two cases of liver fibrosis... Objective: To observe the efficacy of Astragalus Polygonum Anti Fibrosis decoction (APAFD)and Jinshuibao capsule (JSBC) in treating liver fibrosis of chronic hepatitis B. Methods: Ninety two cases of liver fibrosis of chronic hepatitis B were randomly divided into group A and group B. Patients in group A received APAFD for 48 weeks, and in group B, they received JSBC for 48 weeks. The effects on the level change of hyaluronic acid (HA), laminin (LN), pro collagen Ⅲ (PCⅢ) and collagen Ⅳ (CⅣ) as well as liver functional tests and liver biochemical parameters before and after treatment were observed.Results: Level of serum HA, LN, PCⅢ and CⅣ in group A declined more obviously than that of group B, the difference was significant ( P <0.01). The liver functional tests such as total bilirubin (TB), alanine aminotransferase (ALT), albumin/globulin (A/G) ratio, hepatitis related serum biochemical parameters such as cholylglycine (CG), serum ferritin (SF), prealbumin (PC) of group A were all improved more significantly than that of group B ( P <0.01).Conclusion: APAFD is more effective than JSBC in treating liver fibrosis of chronic hepatitis B and in the inhibition of hepatic inflammation, hence it is a good composite Chinese herbal preparation against liver fibrosis. 展开更多
关键词 chronic hepatitis B liver fibrosis Astragalus Polygonum anti fibrosis decoction Jinshuibao capsule
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德都红花-7味散对CCl_4诱导实验性肝纤维化疗效和IL-1、IL-6、TNF-α的影响 被引量:15
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作者 巴图德力根 韩志强 娜日苏 《中国免疫学杂志》 CAS CSCD 北大核心 2012年第4期316-322,共7页
目的:观察德都红花-7味散对CCl4所致实验性肝纤维化疗效和对IL-1、IL-6、TNF-α的影响,探讨该药的作用机制。方法:将大鼠随机分为5组,除空白对照组,其余各组腹腔注射30%四氯化碳橄榄油溶液诱导大鼠形成肝纤维化;同时德都红花-7味散低、... 目的:观察德都红花-7味散对CCl4所致实验性肝纤维化疗效和对IL-1、IL-6、TNF-α的影响,探讨该药的作用机制。方法:将大鼠随机分为5组,除空白对照组,其余各组腹腔注射30%四氯化碳橄榄油溶液诱导大鼠形成肝纤维化;同时德都红花-7味散低、高剂量组和秋水仙碱阳性对照组分别灌胃德都红花-7味散每日0.62 g/kg、1.04 g/kg和秋水仙碱0.4 mg/kg;于第7周末,采集血清和肝组织,酶联免疫吸附试验检测纤维化指标HA、LN、PCⅢ、ⅣC和血清TNF-α、IL-1、IL-6含量,并用HE、Gomori、Masson染色观察肝组织病理学变化,采用2000年西安会议制定的《肝纤维化诊断及疗效评估共识》和《肝纤维化分期半定量评估系统Ishak》对肝纤维化程度进行分级、分期和评分。结果:与模型组比较德都红花-7味散低剂量组血清和肝组织HA、LN、PCⅢ、ⅣC、Hyp含量,炎症活动度及纤维化程度半定量值,血清TNF-α、IL-1、IL-6含量均明显降低,P<0.05;高剂量组血清HA、ⅣC,肝组织LN、ⅣC含量,炎症活动度及纤维化程度半定量值,血清TNF-α含量均明显降低,P<0.05;秋水仙碱组肝组织HA含量升高、ⅣC含量下降,有显著性差异,P<0.05;炎症活动度和纤维化程度半定量值无显著性差异,P>0.05;血清TNF-α、IL-1、IL-6含量升高,有显著性差异,P<0.05。与空白对照组比较德都红花-7味散低剂量组血清HA、PCⅢ、ⅣC,肝组织LN、PCⅢ、ⅣC,德都红花-7味散高剂量组血清PCⅢ、ⅣC,肝组织ⅣC含量无显著性差异,P>0.05。结论:德都红花-7味散能够明显改善CCl4所诱导实验性肝纤维化组织病理变化,降低肝纤维化指标HA、LN、PCⅢ、ⅣC和血清IL-1、IL-6、TNF-α含量,具有抗肝纤维化作用。 展开更多
关键词 德都红花-7味散 四氯化碳 抗肝纤维化
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苦杏仁苷对大鼠肝星状细胞HSC-T6增殖和Bax基因表达的影响 被引量:7
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作者 赵昉 骆欢欢 张奉学 《新中医》 CAS 2012年第1期111-113,共3页
目的:研究苦杏仁苷对大鼠肝星状细胞HSC-T6增殖的影响和对凋亡基因Bax的调控作用。方法:利用噻唑蓝染色法动态检测不同浓度的苦杏仁苷对大鼠HSC-T6细胞增殖的影响,利用PCR法检测苦杏仁苷对HSC-T6细胞凋亡基因Bax的表达水平。结果:苦杏... 目的:研究苦杏仁苷对大鼠肝星状细胞HSC-T6增殖的影响和对凋亡基因Bax的调控作用。方法:利用噻唑蓝染色法动态检测不同浓度的苦杏仁苷对大鼠HSC-T6细胞增殖的影响,利用PCR法检测苦杏仁苷对HSC-T6细胞凋亡基因Bax的表达水平。结果:苦杏仁各实验浓度组对HSC-T6细胞的增殖无直接抑制作用,但其高浓度10-3mol/L和中浓度10-4mol/L可以使HSC-T6细胞提前到达增殖高峰期;而各浓度组对HSC-T6细胞的Bax基因都有一定的诱导作用,并且存在量效关系。结论:苦杏仁苷不能直接抑制HSC-T6细胞的增殖,但可能通过诱导或上调凋亡基因Bax的过度表达,使细胞提前进入凋亡程序阶段,最终使活化的HSC-T6发生细胞凋亡,以起到抗肝纤维化的作用。 展开更多
关键词 苦杏仁苷 肝星状细胞 抗肝纤维化 BAX基因
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美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖的含量测定 被引量:5
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作者 李夸巧 李春艳 +2 位作者 杨永寿 陈丽娜 肖培云 《大理学院学报(综合版)》 CAS 2013年第3期9-12,共4页
目的:建立美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖的含量测定方法。方法:采用苯酚-硫酸法测定粘多糖的含量,采用正交试验设计考察ML-D中粘多糖的水解工艺和显色条件,采用单因素试验考察加热显色时间、苯酚和硫酸的用量。结果:ML-D中... 目的:建立美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖的含量测定方法。方法:采用苯酚-硫酸法测定粘多糖的含量,采用正交试验设计考察ML-D中粘多糖的水解工艺和显色条件,采用单因素试验考察加热显色时间、苯酚和硫酸的用量。结果:ML-D中粘多糖的最佳水解工艺为:盐酸浓度6 mol/L,固液比为1∶1,水解时间1 h;最佳显色条件:苯酚2 mL,硫酸20 mL,加热显色时间10 min。结论:该法操作简便,精密度高,重复性好,可作为美洲大蠊抗肝纤维化活性提取物ML-D中粘多糖含量的测定方法。 展开更多
关键词 美洲大蠊 抗肝纤维化 粘多糖 硫酸-苯酚法 含量测定
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龙胆泻肝汤加减对慢性乙型肝炎低病毒血症期抗纤维化改善、病毒载量及血清HBsAg、ADA的影响
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作者 徐宇萱 牛建海 高欢 《天津中医药》 CAS 2024年第11期1368-1372,共5页
[目的]观察龙胆泻肝汤加减对慢性乙型肝炎低病毒血症期抗纤维化改善、病毒载量及血清乙型肝炎表面抗原(HBsAg)、腺苷脱氨酶(ADA)的影响。[方法]选择2020年1月——2023年1月于医院治疗的102例慢性乙型肝炎患者,随机数字表法分为西医组和... [目的]观察龙胆泻肝汤加减对慢性乙型肝炎低病毒血症期抗纤维化改善、病毒载量及血清乙型肝炎表面抗原(HBsAg)、腺苷脱氨酶(ADA)的影响。[方法]选择2020年1月——2023年1月于医院治疗的102例慢性乙型肝炎患者,随机数字表法分为西医组和中西医组。西医组52例,给予患者恩替卡韦片治疗,中西医组50例,西医组治疗同时给予患者龙胆泻肝汤加减治疗,比较西医组及中西医组临床疗效,治疗前、后给予患者身目俱黄、脘闷腹胀、胁肋疼痛、小便黄赤、烦热、口干而苦、困倦乏力、食欲不振、皮肤瘙痒、恶心呕吐、大便秘结或稀溏评价,检测患者乙型肝炎病毒(HBV)-DNA、HBsAg、ADA、总胆红素、CD4^(+)/CD8^(+)、Ⅲ型胶原、透明质酸、白细胞介素-27(IL-27)水平,计算HBV-DNA、HBsAg转阴率。[结果]中西医组患者总有效率为96.00%,西医组总有效率为80.77%,中西医组患者总有效率较西医组高(P<0.05);中西医组身目俱黄、脘闷腹胀、胁肋疼痛、小便黄赤、烦热、口干而苦、困倦乏力、食欲不振、皮肤瘙痒、恶心呕吐、大便秘结或稀溏评分较西医组降低(P<0.05);中西医组HBV-DNA、HBsAg水平较西医组低(P<0.05),中西医组HBV-DNA、HBsAg转阴率较西医组高(P<0.05);中西医组ADA、总胆红素、Ⅲ型胶原、透明质酸水平较西医组低(P<0.05);中西医组CD4^(+)/CD8^(+)较西医组高(P<0.05),中西医组IL-27水平较西医组低(P<0.05)。[结论]龙胆泻肝汤加减治疗慢性乙型肝炎低病毒血症患者,可改善免疫功能,抑制炎症,提升抗纤维化能力,减少病毒载量及血清HBsAg、ADA水平,缓解病情,提升临床疗效。 展开更多
关键词 龙胆泻肝汤加减 慢性乙型肝炎 低病毒血症 免疫功能 抗纤维化 病毒载量
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拉米夫定联合重组人干扰素α-2b治疗慢性乙型病毒性肝炎的临床疗效 被引量:4
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作者 朱红欣 《中国现代医生》 2017年第3期89-91,94,共4页
目的探讨拉米夫定联合重组人干扰素α-2b治疗慢性乙型病毒性肝炎的临床疗效。方法选择2014年1~12月在我院治疗的慢性乙型病毒性肝炎患者60例为研究对象,随机分为研究组和对照组各30例。两组均给予拉米夫定治疗,研究组在此基础上给予重... 目的探讨拉米夫定联合重组人干扰素α-2b治疗慢性乙型病毒性肝炎的临床疗效。方法选择2014年1~12月在我院治疗的慢性乙型病毒性肝炎患者60例为研究对象,随机分为研究组和对照组各30例。两组均给予拉米夫定治疗,研究组在此基础上给予重组人干扰素α-2b治疗。比较两组治疗后ALT恢复正常率、HBV-DNA转阴率、HBeAg转阴率、HBeAg转化率、总有效率以及不良反应情况。结果治疗后,研究组ALT恢复正常率、HBV-DNA转阴率、HBeAg转阴率、HBeAg转化率均显著高于对照组,差异有统计学意义(P<0.05)。研究组总有效率显著高于对照组,差异有统计学意义(P<0.05)。两组不良反应比较差异无统计学意义(P>0.05)。结论拉米联合夫定重组人干扰素α-2b治疗慢性乙型病毒性肝炎能够显著提高临床疗效,并且未明显增加不良反应,值得临床推广。 展开更多
关键词 拉米夫定 重组人干扰素Α-2B 慢性乙型病毒性肝炎 免疫调节 抗纤维化
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苦瓜中葫芦烷型三萜成分及其抗肝纤维化活性的研究
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作者 岳佳音 徐静 +1 位作者 张晓书 赵余庆 《西北药学杂志》 2024年第1期26-31,共6页
目的研究苦瓜酸水解产物的化学成分及其抗肝纤维化活性。方法用正、反相柱层析等色谱手段进行分离纯化,用波谱技术与文献数据对照鉴定其结构;并用四甲基偶氮唑盐(methylthiazolyl tetrazolium,MTT)法测定部分化合物体外对大鼠肝星状细胞... 目的研究苦瓜酸水解产物的化学成分及其抗肝纤维化活性。方法用正、反相柱层析等色谱手段进行分离纯化,用波谱技术与文献数据对照鉴定其结构;并用四甲基偶氮唑盐(methylthiazolyl tetrazolium,MTT)法测定部分化合物体外对大鼠肝星状细胞(t-HSC/Cl-6)增殖的抑制作用。结果共分离出10个葫芦烷型三萜类化合物,分别鉴定为:5β,19-epoxy-3β-hydroxy-19(R)-methoxy-27-norcucurbita-6,23(E)-dien-25-one(1);5β,19-epoxy-3β-hydroxy-19(S)-methoxy-27-norcucurbita-6,23(E)-dien-25-one(2);5β,19-epoxy-25-methoxycucurbita-6,23(E)-dien-3β-ol(3);karavilagenin A(4);(23E)-5β,19-epoxycucurbita-6,23,25-triene-3β-ol(5);23E-5β,19-epoxy-cucurbita-6,23-dien-3β,25-diol(6);25-O-methylkaravilagein D(7);(23E)-3β-hydroxy-7β,25-dimethoxycucurbita-5,23-dien-19-al(8);karavilagenin B(9);(19S,23E)-5β,19-epoxy-19-methoxycucurbita-6,23-diene-3β,25-diol(10)。体外活性筛选发现化合物1和化合物2对t-HSC/Cl-6有一定的抑制细胞增殖作用,IC50分别为(25.92±2.63)、(26.22±2.77)μmol·L^(-1)。结论化合物1和化合物2为首次从该植物中分离得到,其对肝纤维化细胞增殖有一定的抑制作用。 展开更多
关键词 苦瓜 葫芦烷型三萜化合物 抗肝纤维化活性
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白灵芝提取物对大鼠肝星状细胞增殖、活化及Wnt/β-catenin信号通路的影响 被引量:3
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作者 肖毅力 张宝月 +1 位作者 张亚红 宋正己 《山东医药》 CAS 2021年第5期40-43,共4页
目的观察白灵芝提取物(GLE)对大鼠肝星状细胞增殖、活化的影响,并探讨其是否与Wnt/β-catenin信号通路有关。方法将大鼠肝星状细胞HSC-T6分为GLE组和对照组,GLE组用稀释不同倍数的GLE干预,对照组加等量培养液,分别于干预24、48、72 h用C... 目的观察白灵芝提取物(GLE)对大鼠肝星状细胞增殖、活化的影响,并探讨其是否与Wnt/β-catenin信号通路有关。方法将大鼠肝星状细胞HSC-T6分为GLE组和对照组,GLE组用稀释不同倍数的GLE干预,对照组加等量培养液,分别于干预24、48、72 h用CCK8法测定细胞增殖OD值,计算细胞增殖抑制率,筛选GLE作用时间及作用浓度;将HSC-T6细胞分为干预组和对照组,干预组加入筛选浓度的GLE,对照组加入等量培养液,于筛选的作用时间时,采用RT-PCR法检测HSC活化标志蛋白α平滑肌肌动蛋白(α-SMA)、转化生长因子β1(TGF-β1)mRNA,Western blotting法检测Wnt/β-catenin信号通路关键蛋白Frizzled-4、β-catenin。结果随着GLE组浓度增加及干预时间延长,HSC-T6细胞增殖的OD值逐渐降低,并以48 h、稀释200倍作为GLE后续实验作用时间及浓度。与对照组比较,干扰组HSC-T6细胞α-SMA、TGF-β1 mRNA及Frizzled-4、β-catenin蛋白表达量降低(P均<0.05)。结论GLE可能通过抑制Wnt/β-catenin信号通路的激活抑制HSC-T6的增殖与活化,具有发展为防治肝纤维化临床用药的潜力。 展开更多
关键词 白灵芝提取物 肝星状细胞 细胞增殖 细胞活化 WNT/Β-CATENIN信号通路 抗肝纤维化
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龙血素A对大鼠肝星状细胞增殖及Frizzled-4受体蛋白表达的影响
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作者 胡建鹏 宋正己 +2 位作者 寻琳婷 李霆 赵雪茹 《昆明医科大学学报》 CAS 2016年第6期13-17,共5页
目的研究WNT信号通路中卷曲蛋白-低密度脂蛋白相关蛋白复合受体(Frizzled/LRP)在体外培养活化大鼠肝星状细胞(a HSCs)中的表达及龙血素A(Loureirin A)干预对大鼠肝星状细胞(a HSC)增殖及α-平滑肌肌动蛋白、转化生长因子β1分泌的影响,... 目的研究WNT信号通路中卷曲蛋白-低密度脂蛋白相关蛋白复合受体(Frizzled/LRP)在体外培养活化大鼠肝星状细胞(a HSCs)中的表达及龙血素A(Loureirin A)干预对大鼠肝星状细胞(a HSC)增殖及α-平滑肌肌动蛋白、转化生长因子β1分泌的影响,探讨龙血素A抗肝纤维化的细胞分子机制.方法分离SD大鼠肝星状细胞,体外传代培养诱导细胞活化,倒置相差显微镜下观察肝星状细胞活化前后形态学变化,用不同浓度龙血素A处理活化的大鼠肝星状细胞(a HSCs).MTT法测定龙血素A对肝星状细胞的生长抑制率;Western Blot方法从蛋白水平检测肝星状细胞中Frizzled-4受体蛋白;Elisa测定药物干预后细胞培养上清液中α-SMA和TGFβ1的含量.结果与对照组相比,龙血素A抑制肝星状细胞增殖并有明显的时间-剂量效应关系,IC50=0.30μg/μL;龙血素A在蛋白水平显著下调受体蛋白Frizzled-4表达,同时抑制肝星状细胞分泌α-SMA、TGFβ1(P<0.05).结论 Frizzled-4受体蛋白在活化的大鼠肝星状细胞(a HSCs)中高表达,经龙血素A干预后,Frizzled-4表达量明显降低,细胞增殖受到抑制,α-SMA、TGFβ1合成和分泌量下降,这提示龙血素A可能与WNT信号通路调节肝纤维发生和血管新生的分子机制相关. 展开更多
关键词 龙血素A 肝星状细胞 Frizzled-4受体蛋白 WNT信号通路 抗肝纤维化
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星点设计-响应面法优选美洲大蠊抗肝纤维化颗粒的成型工艺 被引量:5
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作者 谢静静 杨永寿 +1 位作者 郝芳芳 肖培云 《医药导报》 CAS 北大核心 2021年第12期1710-1715,共6页
目的优选美洲大蠊抗肝纤维化颗粒的成型工艺。方法以成型率与吸湿率的总评归一值为考察指标,药辅质量比、辅料质量比、乙醇体积分数为考察因素,通过单因素实验和星点设计-响应面法优选美洲大蠊抗肝纤维化颗粒的成型工艺,并加以验证。结... 目的优选美洲大蠊抗肝纤维化颗粒的成型工艺。方法以成型率与吸湿率的总评归一值为考察指标,药辅质量比、辅料质量比、乙醇体积分数为考察因素,通过单因素实验和星点设计-响应面法优选美洲大蠊抗肝纤维化颗粒的成型工艺,并加以验证。结果美洲大蠊抗肝纤维化颗粒的成型工艺为药辅质量比为1:2.84,微晶纤维素和淀粉质量比为1.37:1,乙醇体积分数77.4%。验证实验中,吸湿率与成型率总评归一值为0.6835,与预测值0.6766的相对误差为1.02%,颗粒物理性能检测符合规定。结论优选的成型工艺稳定可靠,方法简便,颗粒成型性、抗湿性良好,可用于美洲大蠊抗肝纤维化颗粒的制备。 展开更多
关键词 美洲大蠊抗肝纤维化颗粒 星点设计-效应面法 成型工艺
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中医药防治慢性乙型肝炎肝纤维化的现状、挑战与思考 被引量:6
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作者 施梅姐 萧焕明 +3 位作者 谢玉宝 黎胜 曾佛来 池晓玲 《临床肝胆病杂志》 CAS 北大核心 2023年第6期1280-1286,共7页
近年来,中医药防治慢性乙型肝炎肝纤维化的相关研究取得了重大突破。在临床研究方面,产生了数个多中心的随机双盲对照研究,并以肝病理学变化为主要疗效评价指标,提高了中医药防治肝纤维化临床研究的证据级别。在基础研究方面,主要围绕... 近年来,中医药防治慢性乙型肝炎肝纤维化的相关研究取得了重大突破。在临床研究方面,产生了数个多中心的随机双盲对照研究,并以肝病理学变化为主要疗效评价指标,提高了中医药防治肝纤维化临床研究的证据级别。在基础研究方面,主要围绕影响肝星状细胞活化、凋亡与自噬、抑制肝脏炎症损伤、调节免疫、抑制肝窦毛细血管化等多个层面,深入研究了中医药抗肝纤维化的作用机制,突出了中医药“多成分、多靶点、多通路”的作用优势与研究难点。在《中医药振兴发展重大工程实施方案》的指引下,未来如何做更高质量的中医药抗肝纤维化的临床与基础研究,产出更高质量的行业成果,助力中医药抗肝纤维化走向世界是关键。 展开更多
关键词 乙型肝炎 慢性 肝纤维化 抗肝纤维化药(中药)
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