基于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯电化学免疫传感器检测Anti-IgG含量的研究

应用物理吸附法将羊抗人IgG抗原直接固定于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯修饰的金电极表面,制备电化学免疫传感器。采用循环伏安法和交流阻抗法对传感器进行表征,结果表明该传感器适合检测Anti-IgG浓度。同时探讨了缓冲液pH值、扫描速度、免疫反应温度、抗原与抗体配比对循环伏安峰电流的影响,结果表明在5-100mV/s扫速范围内,峰电流与扫速呈线性。孵育最优条件为25℃,h-IgG与Anti-IgG配比为1∶1。循环伏安法研究还表明Anti-IgG浓度在0.01-260μg/L范围内,线性关系良好,相关系数r^2=0.993,检出限（S/N=3）为0.006μg/L,据此建立了检测Anti-IgG浓度的新方法。

信号分子IRAK1/4在antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF中的作用探讨

目的:探讨IRAK1和IRAK4在antiβ-2GPI/β2GPI复合物诱导单核细胞株THP-1表达组织因子(TF)中的作用。方法:利用荧光定量PCR(Real-time PCR)、TF活性试剂盒分别检测THP-1细胞表达TF mRNA及TF活性;Western blot检测anti-β2GPI/β2GPI复合物诱导THP-1细胞表达IRAK1、磷酸化-IRAK1(p-IRAK1)、IRAK4情况;观察IRAK1/4抑制物是否干预anti-β2GPI/β2GPI复合物诱导THP-1表达TF。结果:Antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1细胞表达TF显著增加(P<0.05 vs control);Antiβ-2GPI/β2GPI复合物(100μg/ml)刺激THP-1细胞表达IRAK1、p-IRAK1、IRAK4(蛋白)显著升高(P<0.05 vscontrol);IRAK1/4抑制物(50μmol/L)能够阻断antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1表达TF及IRAK1磷酸化的效应。结论:antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF过程中,信号分子IRAK1/4被激活进而发挥重要作用。

Abnormal Secondary Growth and Histochemical Localization of Alkaloids in Root System of Aconitum flavum Hand.-Mazz.

[Objective] The purpose of this study was to clarify the structure,growth pattern and histochemical localization of alkaloids in root system of Aconitum flavum Hand.-Mazz.[Method] Paraffin sectioning and histochemistry were employed for performing the analysis in this study.[Result] The root system of Aconitum flavum Hand.-Mazz.consists of taproot,lateral root and adventitious root.The primary structure of root system is normal,but secondary structure shows abnormal.The cambium and the extra cambium of taproot form a ＂U＂-shaped secondary vascular bundle and tertiary bundle in abnormal secondary structure.The sieve tube group is made of little sieve tube group which is differentiated from primary phloem and cambium.Meanwhile,the secondary xylem in tuberous root also appears to be a ＂U＂ shape.Parenchyma cells of secondary phloem occupy most of the tuberous root.The sieve tube group of tuberous root is mainly differentiated from parenchyma cell of secondary phloem.[Conclusion] The difference in abnormal secondary structure of taproot and tuberous root are attributed to their varied cambium compose and activity pattern.Alkaloids are mainly accumulated in parenchyma cell of the inside cortex and between bundle in taproot,while parenchyma of secondary phloem and pith in tuberous root.

三维Anti de Sitter空间中Lorentzian曲面的S_t^1×S_s^1-值光锥Gauss映射的奇点分类

利用Arnol'd的Legendrian理论,对三维Anti de Sitter空间中Lorentzian曲面进行了研究.引入光维高度函数概念研究了三维Anti de Sitter空间Lorentzian曲面的S1t×S1s-值、光锥Gauss映射的奇点,进行了奇点分类,揭示了类光Causs-kronecker曲率之间的关系;并研究了Lorentzian曲面的一些基本几何性质.

A meshfree-based local Galerkin method with condensation of degree of freedom for elastic dynamic analysis

Condensation technique of degree of freedom is first proposed to improve the computational efficiency of meshfree method with Galerkin weak form for elastic dy- namic analysis. In the present method, scattered nodes with- out connectivity are divided into several subsets by cells with arbitrary shape. Local discrete equation is established over each cell by using moving Kriging interpolation, in which the nodes that located in the cell are used for approxima- tion. Then local discrete equations can be simplified by con- densation of degree of freedom, which transfers equations of inner nodes to equations of boundary nodes based on cells. The global dynamic system equations are obtained by as- sembling all local discrete equations and are solved by using the standard implicit Newmark＇s time integration scheme. In the scheme of present method, the calculation of each cell is carried out by meshfree method, and local search is imple- mented in interpolation. Numerical examples show that the present method has high computational efficiency and good accuracy in solving elastic dynamic problems.

Deformation Localization-A Review on the Maximum-Effective-Moment(MEM) Criterion

The essential difference in the formation of conjugate shear zones in brittle and ductile deformation is that the intersection angle between brittle conjugate faults in the contractional quadrants is acute （usually ～60°） whereas the angle between conjugate ductile shear zones is obtuse （usually 110°）. The Mohr-Coulomb failure criterion, an experimentally validated empirical relationship, is commonly applied for interpreting the stress directions based on the orientation of the brittle shear fractures. However, the Mohr-Coulomb failure criterion fails to explain the formation of the low-angle normal fault, high-angle reverse fault, and the conjugate strike-slip fault with an obtuse angle in the ~1 direction. Although it is ten years since the Maximum-Effective-Moment （MEM） criterion was first proposed, and increasingly solid evidence in support of it has been obtained from both observed examples in nature and laboratory experiments, it is not yet a commonly accepted model to use to interpret these anti- Mohr-Coulomb features that are widely observed in the natural world. The deformational behavior of rock depends on its intrinsic mechanical properties and external factors such as applied stresses, strain rates, and temperature conditions related to crustal depths. The occurrence of conjugate shear features with obtuse angles of -110~ in the contractional direction on different scales and at different crustal levels are consistent with the prediction of the MEM criterion, therefore -110° is a reliable indicator for deformation localization that occurred at medium-low strain rates at any crustal levels. Since the strain-rate is variable through time in nature, brittle, ductile, and plastic features may appear within the same rock.

On the Construction of Deepening Anti - corruption Mechanism

It needs the foundation of system and the guarantee of organizational system for anti-corruption,but it is more necessary to build and form an effective anti-corruption mechanism,so that the anti-corruption can be really put into practice. Anti-corruption mechanism refers to a organic operation system of the interaction,interconnection and constraint between the constituent elements( parts) and elements of national anti-corruption,and as a system,anti-corruption mechanism should have the characteristics of system aticness,comprehensiveness,transparency,legalization,public participation,scientific dynam ic,and internationalism. The construction of deepening anti-corruption mechanism is the need for reconstructing the ruling legitimacy of the party and the governm ent. Adhering to the principle of treating both root causes and symptoms is necessary in the construction of anti-corruption m echanism,com bating and punishing corruption is an important part of anti-corruption,and the prevention and control of corruption is the basic project of anti-corruption. Therefore,the construction of prevention and control mechanism in the anti-corruption mechanism has a more far-reaching significance.

Nimotuzumab with Concurrent Chemo-Radiotherapy in Patients with Locally Advanced Squamous Cell Carcinoma of Head and Neck (LASCCHN)

Background: Head and neck cancers (HNCs) constitute 5% of all cancers globally and are the most common cancers in India. Chemotherapy and radiotherapy have not been proved to be effective in advanced cases and the prognosis remains dismal. This underscores the need for newer treatment options in these cases. Nimotuzumab, an anti-epidermal growth factor receptor (anti-EGFR) monoclonal antibody, was safer when combined with chemo- or radio-therapy. Aim: To evaluate the safety and efficacy of concurrently administered nimotuzumab with chemo-radiotherapy in patients with advanced inoperable squamous cell carcinomas of head and neck (LASCCHN). Methods:?This was an open-label, single arm study evaluating 57 patients with histologically confirmed inoperable LASCCHN (stages III and IV) and eastern co-operative oncology group (ECOG) performance status < 2. Informed consent was obtained from all patients. The patients were administered IV cisplatin 30 mg/m2?and IV nimotuzumab 200 mg weekly for 6 weeks, along with radiotherapy of 6600 cGy over 33 fractions. Patients were evaluated over response evaluation criteria in solid tumors (RECIST) criteria 24 weeks after the last cycle of chemotherapy. Results: Mean age of patient was 50 years old (29 - 79 years old). The most common site of cancer was oral cavity (56.1%). Forty six patients (80.7%) completed 6 cycles of therapy. Objective response rate (ORR) was 80.7%, with 34 patients (59.6%) achieving complete response (CR), and 12 (21%) achieving partial response (PR). Stable disease (SD) was noted in 8 (14%) patients and progressive disease in 3 (5.2%) patients. Conclusion: Addition of nimotuzumab is a safe and efficacious option in patients with inoperable LASCCHN. Our observations confirm the available Phase II data. The long term survival benefits based on this encouraging response rate need to be further evaluated in this subset of cancer patients.

Investigations of the electron paramagnetic resonance parameters and the tetragonal local structure for (VCl_6)^(4-) coordination complex in MCl:V^(2+) (M=Na,K,Rb) systems

By simulating the electron paramagnetic resonance （EPR） and optical spectra on the basis of the 120 × 120 complete energy matrix, this paper determines the local lattice structure parameters R1 and R2 for MCl：V2＋ （M=Na, K, Rb） systems at 77K, 195 K and RT （room temperature 295 K or 302 K）, respectively. The theoretical results indicate that there exists a compressed distortion in MCl：V2＋ systems. Meanwhile, it finds that the structure parameters R1, R2 and ｜△R｜（ = R1 - R2） increase with the rising temperature. Subsequently, from the analysis it concludes that the relation of EPR parameter D vs. △R is approximately linear. Finally, the effects of orbital reduction factor k on the g factors for the three systems have been discussed.

新辅助抗PD-1免疫治疗联合放化疗治疗局部晚期非小细胞肺癌的临床疗效

目的研究新辅助抗PD-1免疫疗法与化疗结合放疗序贯治疗方案治疗局部晚期非小细胞肺癌的临床疗效与安全性。方法回顾性选取2021年3月至2022年3月江苏省肿瘤医院治疗的非小细胞肺癌患者80例。依据治疗方案不同分为研究组和对照组,每组各40例。研究组接受含新辅助抗PD-1疗法的综合放化疗,对照组仅接受标准放化疗。比较两组患者的实体瘤治疗客观缓解率、细胞免疫功能、血清肿瘤因子[癌胚抗原(CEA)、糖类抗原(CA)125、CA199]以及不良反应。结果研究组患者治疗后的客观缓解率为7.00%,明显高于对照组(52.50%),差异有统计学意义(P<0.05)。治疗后,研究组患者的CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)均较治疗前升高,对照组患者的CD3^(+)、CD4^(+)、CD4^(+)/CD8^(+)均较治疗前降低,且研究组患者的CD3^(+)、CD4^(+)、CD8^(+)、CD4^(+)/CD8^(+)分别为(64.33±5.16)%、(43.71±4.53)%、(32.54±3.91)%、1.34±0.26,均明显高于对照组[(55.25±5.63)%、(31.87±3.16)%、(28.24±3.89)%、1.13±0.31],差异均有统计学意义(P<0.05)。治疗后,两组患者的血清CEA、CA125、CA199水平均较治疗前降低,且研究组患者的血清CEA、CA125、CA199水平分别为(12.25±4.15)ng/mL、(31.55±5.78)U/mL、(56.62±8.52)U/mL,均明显低于对照组[(18.97±4.36)ng/mL、(38.17±5.81)U/mL、(65.20±8.79)U/mL],差异均有统计学意义(P<0.05)。两组患者不良反应发生率比较,差异无统计学意义(P>0.05)。结论新辅助抗PD-1免疫治疗联合放化疗相比单纯放化疗对局部晚期非小细胞肺癌有更加明显的优势,能够提高放化疗过程中的机体免疫功能,减少血清肿瘤相关因子,同时没有增加明显的不良反应,可以作为非小细胞肺癌提升疗效的方案在临床中推广应用。

Anti-Bacterial and Flame Retardant for Cotton Fabric by One-Bath Finishing

A research on the process of cotton fabric flame-re-tarding,anti-bacterial finishing and one-bath finish-ing of anti-bacterial and flame-retarding is discussed.The flame retardant agent was phosphorous-contained,and the bacteriostatic finishing agent named SFR-1 wassynthesized.The flame retardancy of the fabric finishedcan meet the DOC FF3-71 Children Sleepwear Stan-dard.Its bacterial inhibiting capacity can meet and ex-ceed the requirements of similar products

Testing of Anti-stripping Property of Friction Spun Core Yarn

Friction spun core yarn has two components: filament core and staple fiber sheath. Under axial rubbing action, the failure mode of the core yarn is the stripping of the sheath from the core. This paper introduces a method to test the anti - stripping property of the core yarn. With a modified Universal Testing Machine, the stripping resistance of friction spun core yarn can be continuously measured. Some factors Influencing the measurements are discussed in detail. The testing results are compared with those from a Y731 Yarn Abrasion Tester and fur - ther confirmed by weaving practice.

局部晚期乳腺癌患者新辅助化疗前后p53、Ki-67、NM23、EGFR的表达变化及临床意义

目的探讨局部晚期乳腺癌患者新辅助化疗前后p53、Ki-67、NM23、表皮生长因子受体(EGFR)的表达变化及临床意义。方法收集112例Ⅱ～Ⅲ期乳腺癌患者的临床资料,所有患者均接受氟尿嘧啶+表柔比星+环磷酰胺序贯多西他赛(FEC-T)或多西他赛+表柔比星+环磷酰胺(TEC)化疗,化疗前后行空心针活检穿刺获得病理标本,采用免疫组织化学法检测p53、Ki-67、NM23、EGFR的表达情况,比较化疗前后上述蛋白的阳性表达情况及其与化疗疗效的关系。采用Logistic回归模型分析乳腺癌患者化疗疗效的影响因素。分析化疗前p53、Ki-67、NM23、EGFR阳性表达情况与患者3年生存率的关系。结果化疗后乳腺癌患者的p53、Ki-67、EGFR阳性表达率均低于化疗前,NM23阳性表达率高于化疗前,差异均有统计学意义(P﹤0.05)。化疗后有效患者75例,化疗前p53、Ki-67、EGFR阴性患者的有效率高于p53、Ki-67、EGFR阳性患者,而NM23阴性患者的有效率低于NM23阳性患者,差异均有统计学意义(P﹤0.05)。Logistic多因素分析结果显示,组织学分级为Ⅲ级及p53、Ki-67、EGFR阳性表达是影响患者化疗疗效的危险因素,NM23阳性表达是影响患者化疗疗效的保护因素(P﹤0.05)。化疗前p53、Ki-67、EGFR阳性患者的3年生存率均低于p53、Ki-67、EGFR阴性患者,NM23阳性患者的3年生存率高于NM23阴性患者,差异均有统计学意义(P﹤0.05)。结论局部晚期乳腺癌患者新辅助化疗前后p53、Ki-67、NM23、EGFR的阳性表达率均有变化,且化疗前上述指标的表达情况与化疗后疗效及远期预后有关。

论我国反恐法律体系的整体完善--从新疆、浙江等四地《反恐怖主义法》实施办法展开分析

新疆等四地反恐实施办法的出台,对《反恐怖主义法》中的原则性规定进行了细化,弥补了部分领域反恐监管缺失的不足,发挥了吸收地方反恐经验、回应各地反恐需求等作用,但同时也存在对实践难点回应力度不足、程序性规定细化有限等明显局限。就我国反恐法律体系的整体完善而言,应在反恐理念更新的基础上进一步修改完善《反恐怖主义法》及其他配套法律法规,反恐地方立法则宜遵循“有所为有所不为”的基本立场而着重呈现其特色性与操作性优势。

超载作用下边坡-抗滑桩体系稳定性及土拱效应研究

为深入研究坡顶超载环境下抗滑桩加固边坡的整体稳定性,依据现行地基规范并结合地基承载力理论,取均布超载强度q计算抗滑桩-边坡体系的安全系数和桩身内力分布。在此基础上,讨论坡顶超载外边缘线至坡顶不同水平距离Lq对加固后边坡稳定性的影响,将边坡顶部均布超载视为埋深为零的条形基础,考察不同超载强度和超载作用位置条件下边坡的整体稳定性及桩间土拱效应。研究表明:抗滑桩-边坡体系在特定的桩间距范围内形成土拱,坡顶不同超载强度和超载作用位置影响着桩间土拱的分布、发展及破坏规律,土拱效应集中反映了抗滑桩的作用机制和加固效果。

TUHF代数上的等距和2-局部等距

给出了TUHF代数上的等距的具体表达形式,进一步证明了TUHF代数上满的2-局部等距为线性。

地方优秀文化融入思想政治理论课的路径研究--以东北抗联精神为例

中华民族精神的深刻内涵蕴含在各地方优秀文化之中,弘扬中华民族精神,就是要大力传承地方优秀文化。地方优秀文化所承载的深厚价值内涵与意义底蕴铭刻着人们对美,善、崇高等的向往和追求,它通过影响人,塑造人,激励人的方式来实现“以文化人”。思想政治理论课是传播中华民族精神的主渠道,把地方优秀文化融入思想政治理论课中就成了应有之义。只有深入发掘地方优秀文化的深刻内涵、结合时代要求拓展地方优秀文化的外延、打造地方优秀文化遗址遗迹的实践教学基地,融地方优秀文化于校园文化建设中,才能使地方优秀文化在思想政治理论课中的功能得以充分发挥,才能使地方优秀文化的价值内涵与意义底蕴在教育对象中入脑、入心入行。

基于FS-LBP特征的人脸活体检测方法

针对人脸识别系统无法识别人脸图像是否来自真人的问题,首先改进了傅里叶频谱(Fourier spectrum,FS)特征的人脸活体检测方法,并验证了局部二值模式(local binary pattern,LBP)特征和灰度共生矩阵(gray-level co-occurrence matrix,GLCM)特征人脸活体检测性能。在此基础上,提出了融合LBP特征的FS-LBP特征人脸活体检测算法。实验结果表明,提出的FS-LBP特征在多数据库的混合数据的准确率高达83.17%,更优于多尺度局部二值模式(multiscale local binary pattern,MSLBP)特征。

日本朱子学与反朱子学之关系--伊藤仁斋及其周边

江户时代朱子学与反朱子学的正式对抗,始于伊藤仁斋。在这一时期日本出现了两股思潮:对朱子学纯粹化的真正追求与反朱子学的思潮兴起,即朱子学与反朱子学同时并行。其中反朱子学有一共同的思想特征,即否定朱子学的理气论而以气为核心,又以"性"为"气质之性"。仁斋与素行皆以朱子学为基础,同时又对朱子学展开批判。仁斋及益轩受到明代吴廷翰及郝敬等"气思想家"的影响,然而仁斋将天道与人道加以分离,主张人道的独立性,故而与"气思想家"在思想结构上存在着差异。仁斋与荻生徂徕都否定以理、气、心等观念为共同前提的天道人道一以贯之的主张。在江户时代前,日本人难以对日常价值观进行思想性的表达,而古学派思想家在以朱子为敌之同时,利用朱子学的问题意识以及思想表现的手段,最终实现了思想表达的理论化。

Different strategies of treatment for uterine cervical carcinoma stage ⅠB2-ⅡB

Uterine cervical cancer is the second most common gynecological malignancy. It is estimated that over 35% of tumors are diagnosed at locally advanced disease, stage ⅠB2-ⅡB with an estimated 5-year overall survival of 60%. During the last decades, the initial treatment for these women has been debated and largely varies through different countries. Thus, radical concurrent chemoradiation is the standard of care in United Sated and Canada, and neoadjuvant chemotherapy followed by radical surgery is the first line of treatment in some institutions of Europe, Asia and Latin America. Until today, there is no evidence of which strategy is better over the other. This article describe the evidence as well as the advantages and disadvantages of the main strategies of treatment for women affected by uterine cervical cancer stage ⅠB2-ⅡB.