Exosomes derived from microglia overexpressing miR-124-3p alleviate neuronal endoplasmic reticulum stress damage after repetitive mild traumatic brain injury

We previously reported that miR-124-3p is markedly upregulated in microglia-derived exosomes following repetitive mild traumatic brain injury.However,its impact on neuronal endoplasmic reticulum stress following repetitive mild traumatic brain injury remains unclear.In this study,we first used an HT22 scratch injury model to mimic traumatic brain injury,then co-cultured the HT22 cells with BV2 microglia expressing high levels of miR-124-3p.We found that exosomes containing high levels of miR-124-3p attenuated apoptosis and endoplasmic reticulum stress.Furthermore,luciferase reporter assay analysis confirmed that miR-124-3p bound specifically to the endoplasmic reticulum stress-related protein IRE1α,while an IRE1αfunctional salvage experiment confirmed that miR-124-3p targeted IRE1αand reduced its expression,thereby inhibiting endoplasmic reticulum stress in injured neurons.Finally,we delivered microglia-derived exosomes containing miR-124-3p intranasally to a mouse model of repetitive mild traumatic brain injury and found that endoplasmic reticulum stress and apoptosis levels in hippocampal neurons were significantly reduced.These findings suggest that,after repetitive mild traumatic brain injury,miR-124-3 can be transferred from microglia-derived exosomes to injured neurons,where it exerts a neuroprotective effect by inhibiting endoplasmic reticulum stress.Therefore,microglia-derived exosomes containing miR-124-3p may represent a novel therapeutic strategy for repetitive mild traumatic brain injury.

基于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯电化学免疫传感器检测Anti-IgG含量的研究

应用物理吸附法将羊抗人IgG抗原直接固定于3,5-二溴水杨醛席夫碱镍配合物-氧化石墨烯修饰的金电极表面,制备电化学免疫传感器。采用循环伏安法和交流阻抗法对传感器进行表征,结果表明该传感器适合检测Anti-IgG浓度。同时探讨了缓冲液pH值、扫描速度、免疫反应温度、抗原与抗体配比对循环伏安峰电流的影响,结果表明在5-100mV/s扫速范围内,峰电流与扫速呈线性。孵育最优条件为25℃,h-IgG与Anti-IgG配比为1∶1。循环伏安法研究还表明Anti-IgG浓度在0.01-260μg/L范围内,线性关系良好,相关系数r^2=0.993,检出限（S/N=3）为0.006μg/L,据此建立了检测Anti-IgG浓度的新方法。

信号分子IRAK1/4在antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF中的作用探讨

目的:探讨IRAK1和IRAK4在antiβ-2GPI/β2GPI复合物诱导单核细胞株THP-1表达组织因子(TF)中的作用。方法:利用荧光定量PCR(Real-time PCR)、TF活性试剂盒分别检测THP-1细胞表达TF mRNA及TF活性;Western blot检测anti-β2GPI/β2GPI复合物诱导THP-1细胞表达IRAK1、磷酸化-IRAK1(p-IRAK1)、IRAK4情况;观察IRAK1/4抑制物是否干预anti-β2GPI/β2GPI复合物诱导THP-1表达TF。结果:Antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1细胞表达TF显著增加(P<0.05 vs control);Antiβ-2GPI/β2GPI复合物(100μg/ml)刺激THP-1细胞表达IRAK1、p-IRAK1、IRAK4(蛋白)显著升高(P<0.05 vscontrol);IRAK1/4抑制物(50μmol/L)能够阻断antiβ-2GPI/β2GPI复合物(100μg/ml)诱导THP-1表达TF及IRAK1磷酸化的效应。结论:antiβ-2GPI/β2GPI复合物诱导THP-1细胞表达TF过程中,信号分子IRAK1/4被激活进而发挥重要作用。

三维Anti de Sitter空间中Lorentzian曲面的S_t^1×S_s^1-值光锥Gauss映射的奇点分类

利用Arnol'd的Legendrian理论,对三维Anti de Sitter空间中Lorentzian曲面进行了研究.引入光维高度函数概念研究了三维Anti de Sitter空间Lorentzian曲面的S1t×S1s-值、光锥Gauss映射的奇点,进行了奇点分类,揭示了类光Causs-kronecker曲率之间的关系;并研究了Lorentzian曲面的一些基本几何性质.

On the Construction of Deepening Anti - corruption Mechanism

It needs the foundation of system and the guarantee of organizational system for anti-corruption,but it is more necessary to build and form an effective anti-corruption mechanism,so that the anti-corruption can be really put into practice. Anti-corruption mechanism refers to a organic operation system of the interaction,interconnection and constraint between the constituent elements( parts) and elements of national anti-corruption,and as a system,anti-corruption mechanism should have the characteristics of system aticness,comprehensiveness,transparency,legalization,public participation,scientific dynam ic,and internationalism. The construction of deepening anti-corruption mechanism is the need for reconstructing the ruling legitimacy of the party and the governm ent. Adhering to the principle of treating both root causes and symptoms is necessary in the construction of anti-corruption m echanism,com bating and punishing corruption is an important part of anti-corruption,and the prevention and control of corruption is the basic project of anti-corruption. Therefore,the construction of prevention and control mechanism in the anti-corruption mechanism has a more far-reaching significance.

Anti-Bacterial and Flame Retardant for Cotton Fabric by One-Bath Finishing

A research on the process of cotton fabric flame-re-tarding,anti-bacterial finishing and one-bath finish-ing of anti-bacterial and flame-retarding is discussed.The flame retardant agent was phosphorous-contained,and the bacteriostatic finishing agent named SFR-1 wassynthesized.The flame retardancy of the fabric finishedcan meet the DOC FF3-71 Children Sleepwear Stan-dard.Its bacterial inhibiting capacity can meet and ex-ceed the requirements of similar products

Testing of Anti-stripping Property of Friction Spun Core Yarn

Friction spun core yarn has two components: filament core and staple fiber sheath. Under axial rubbing action, the failure mode of the core yarn is the stripping of the sheath from the core. This paper introduces a method to test the anti - stripping property of the core yarn. With a modified Universal Testing Machine, the stripping resistance of friction spun core yarn can be continuously measured. Some factors Influencing the measurements are discussed in detail. The testing results are compared with those from a Y731 Yarn Abrasion Tester and fur - ther confirmed by weaving practice.

随机重频激光制导雷达抗高重频干扰技术

为了能有效对抗高重频干扰,在分析高重频干扰破坏激光制导雷达信号处理机理的基础上,采用了随机重频照射激光波形,并利用激光目标指示器、目标和探测器的先验距离、速度特征,提出了基于自适应交叠波门的高重频干扰信号排查和鉴别方法,有效控制了重频干扰对目标捕获环节的影响,形成一套从波形设计到信号处理、目标捕获的端到端的抗高重频干扰解决方案,并进行了理论分析和实验验证。结果表明,在不同干扰场景下,有效捕获目标的时间均低于0.2 s,捕获时间在亚秒量级,与无干扰背景下目标典型捕获时间相当。此技术可有效对抗数十赫兹到250 kHz的固定/随机重频干扰,有利于提高制导武器对抗重频干扰的能力。

<i>In Vivo</i>and <i>in Vitro</i>Evaluation of Anti Diabetic and Insulin Secretagogue Activities of <i>Capparis zeylanica</i>

Since ancient times, traditional medicines have been in the usage for the treatment of Diabetes mellitus. An edible fruit from traditional medicinal plant Capparis zeylanica (CZ) was studied for its anti diabetic, insulin secretagogue activities and mechanisms involved in it. In Streptozotocin induced diabetes rats, oral administration of Capparis zeylanica methanolic extract (CZME) (200 mg/kg body weight) for 28 days showed a significant reduction in blood glucose levels by 35.53% and enhanced circulating insulin levels by 81.82% than the diabetic control rats. The insulin secretagogue activity mechanisms of the extract were evaluated by using mouse insulinoma beta cell line (MIN6-β). The extract stimulated insulin release in dependent manner of glucose concentration (3 - 16.7 mM) and extract dose (5 - 500 μg/mL). The insulin releasing effect of the extract was significantly enhanced by 3-isobutyl-1-methyl xanthine, glibenclamide, elevated extracellular calcium and K+ depolarized media. This insulin release was significantly reduced in calcium blocking conditions (by nifedipine and EGTA), in the presence of potassium channel opener (diazoxide). Hence, anti diabetic activity of CZME might be a result of its stimulatory effect on insulin release from pancreatic beta cells via KATP channel dependent and independent ways. These results indicate that CZ fruits have the potential to use in diabetes therapy.

Exact Traveling Wave Solutions of Nano-Ionic Solitons and Nano-Ionic Current of MTs Using the exp(-φ (ξ ))-Expansion Method

In this work, the exp(-φ (ξ )) -expansion method is used for the first time to investigate the exact traveling wave solutions involving parameters of nonlinear evolution equations. When these parameters are taken to be special values, the solitary wave solutions are derived from the exact traveling wave solutions. The validity and reliability of the method are tested by its applications to Nano-ionic solitons wave’s propagation along microtubules in living cells and Nano-ionic currents of MTs which play an important role in biology.

低截获雷达抗分选信号设计技术研究综述

雷达是获取战场态势的关键装备,极易遭受电子攻击。低截获雷达能从根本上提高雷达电子防御能力,是当前雷达研究热点,其重点之一是抗分选信号设计。首先阐述了低截获雷达的概念,然后介绍了低截获雷达的应用需求,说明了低截获雷达抗分选信号设计的必要性和紧迫性。梳理了近年来低截获雷达抗分选信号设计的方法,重点分析了信号组合、信号参数调制、基于脉冲重复周期(Pulse Repetition Interval,PRI)、优化算法四种抗分选信号设计方法。最后展望了抗分选信号设计技术的发展方向。

基于重频滑变的抗分选信号设计

随着无线装备的大量应用,电子战逐渐成为打赢现代战争的关键;提高雷达的抗侦察性能是增强雷达电子对抗的重要手段,而抗分选信号设计能够从根本上增强雷达的抗侦察能力。针对目前在信号侦察领域广泛应用的序列差值直方图算法,从破坏分选算法对信号脉冲重复间隔估计值的角度设计抗分选信号。首先梳理了序列差值直方图分选算法工作步骤,分析了算法对信号分选的关键步骤,然后提出了信号设计的方案,最后进行仿真验证。仿真结果显示所设计的信号可以有效对抗分选算法。

The effect of pre-existing memory representations on repetitionrelated N250r and N400

Studies of repetition priming have found two face-sensitive event-related potential(ERP) components:the N250 r showing positive deflection at frontal region and negative deflection at temporal region, and the N400 showing positive deflection at frontal and centro-parietal regions, both of which depend in part upon the presence or absence of a pre-existing face representation. However, the N250 r is rarely reported for a repetition interval between immediate repetition and 3 min; in addition, whether different types of representations function in the same way is also of interest. The goal of the present experiment is to compare the ERP patterns for faces versus letter strings as a function of the pre-existing memory representation with a repetition interval of 1.5 min on average. We found reliable frontally positive N250 r and N400 for famous faces and words; marginally significant effects for pseudo-words;and only the centro-parietal N400 for unfamiliar faces.Collectively, the N250 r persists in the present intermediate intervals, and both the frontal N250 r and the frontal N400 are domain-general, sensitive to the pre-existing memory representation.

800kV/100Hz高功率微波驱动电源控制系统

为了减少人为因素引起的误动作,使整个电源的运行更加安全可靠,研制了一套800kV重复频率高功率微波驱动电源计算机自动控制系统,实现了电源从手动控制到自动控制的转变。采用PC机与PLC及ADAM模块通讯、虚拟仪器等技术,实现了对触发控制系统、直流高压系统和远程开关机系统的控制。采用光电隔离、多层屏蔽和软硬件滤波等抗干扰措施解决了因电磁干扰强而引起的地电位抬高、触发控制系统损坏、闸流管连动等问题。该系统在上百万次的试验中未出现过一次误触发、误动作,具有很高的可靠性和抗干扰能力。

三相四桥臂并网逆变器重复控制积分抗饱和策略

在新能源并网逆变器中,基于内模原理的重复控制器被广泛应用,但是由于受到功率管开关特性和直流母线电压的约束,重复控制器存在积分饱和现象,严重影响供电质量。为提高三相四桥臂并网逆变器的并网电流质量,基于模型恢复积分抗饱和方法,研究重复控制器积分抗饱和策略。首先,设计了具有积分作用的重复控制器;其次,根据被控对象的数学模型设计了重复控制积分抗饱和补偿器,对控制量和输出反馈量分别进行补偿,控制量补偿引入控制量饱和偏差,并采用线性二次型最优控制方法优化偏差系数;最后,通过仿真和实验验证了重复控制积分抗饱和策略在动态和稳态饱和情况下均能有效改善系统性能。

基于PRI参数的雷达信号抗分选分析

为满足未来综合电子战的需要,在不降低信号功率的条件下,从破坏信号分选所利用的信号规律性出发,提出如何利用PRI参数变化实施信号抗分选的方法。选择基于软件的信号预处理分选方法SIDF作为抗识别效果分析工具,探寻具有抗分选效果好的雷达信号特点,为雷达反侦察研究提供新的思路。

基于迭代学习控制的桥式起重机定位及防摆

针对桥式起重机在小车运行时引起负载摆动,影响工作效率、易引发事故和重复运行时的规律优化控制问题,提出利用迭代学习控制来对桥式起重机进行定位及防摆控制。用拉格朗日(Lagrange)方程建立桥式起重机的数学模型,对桥式起重机进行定位防摆控制仿真。结果表明,迭代学习控制可以利用桥式起重机重复运行的特性来对其进行高精度跟踪,同时有效地抑制负载摆角。

桥式起重机定位及防摆的最优迭代控制

在起重机定位防摇优化控制研究中,由于桥式起重机定位防摆控制系统是一个强耦合、非线性模型复杂系统,系统的实时性能差,传统的PID控制器往往难以进行高精度定位,防摇控制也并不理想。为解决桥式起重机运行的规律优化控制,提出应用最优迭代学习控制的方法,实现对系统的定位以及防摇精确控制。用拉格朗日(Lagrange)方程建立桥式起重机的数学模型和最优迭代学习控制模型,对桥式起重机进行定位防摇控制仿真。结果证明改进的控制算法有效,并通过仿真为桥式起重机精确定位,防摇控制提供了科学依据。

试论《封神演义》的“重复”与“反重复”

《封神演义》是一部富有争议性的古典小说。笔者论述了它的"重复"问题,认为:一、重复并不会影响其成为经典文学;二、有时模式化的叙事可以起到反复加强、给读者留下极深印象的积极作用。此外,还重点论述了《封神演义》的"反重复",认为其"反重复"主要是通过意外叙事来实现的:一、叙事上会使用饶有趣味的小技巧,插入小情节;二、讲究战斗的多样性与复杂性。其"反重复"叙事让读者感到新鲜意外,保持较旺盛的阅读精力与兴趣。

基于分数阶PID和重复控制的快速刀具伺服系统

针对永磁直线同步电机(PMLSM)驱动的快速刀具伺服系统在高频输入信号时的系统跟踪精度问题,设计了一种分数阶PID重复控制算法.由于系统的参考信号和干扰信号都具有周期性的特点,通过对系统参考信号的跟踪和扰动的抑制研究,将改进型分数阶PID控制和重复控制相结合,利用重复控制对周期性输入或复杂干扰信号的跟踪和抑制能力来补偿分数阶PID控制器,使系统有快速响应能力的同时具有较强的抗干扰性.仿真结果表明,所提出的控制策略有效地提高了伺服系统的跟踪精度,并对周期性扰动有较好的抑制作用.