Introduction: Prostate cancer is the second most common cancer in men. The diagnosis is most often based on the prostate biopsies’ analysis and on histological criteria recognizable on standard coloring. In some case...Introduction: Prostate cancer is the second most common cancer in men. The diagnosis is most often based on the prostate biopsies’ analysis and on histological criteria recognizable on standard coloring. In some cases, the use of immunohistochemistry is important. Objectives: This paper aims to specify the p63 phenotypic profile of lesions diagnosed benign, with minimal suspect foci, difficult to interpret, HGPIN (high grade intraepithelial neoplasia) and LGPIN (low-grade prostatic intraepithelial neoplasia) and evaluate the manual technique of p63 immunohistochemistry. Patients and Method: This was a retrospective, descriptive study of prostate biopsies recorded in the PAC service of the HALD from January 1st, 2018 to December 31st, 2018. It was completed by a manual immunohistochemical study of the blocks enrolled from November 19th to December 4th, 2020 in the PAC department of the HPD. The studied parameters were: registry number, age, clinical stage, prostate volume, PSA level, microscopic appearance and p63 immunohistochemical profile. Results: Our study included 60 prostate biopsies. The ages of our patients varied from 45 to 77 years, with an average of 64.2 years and a standard deviation of 6.2. The majority of patients were at clinical stage cT2b (33%) with a prostate volume varying between 33.15 and 169.4 cc. The minimum value of PSA in our series is 5 ng/ml, the maximum being 100 ng/ml with an average level of 24.1 ng/ml and a standard deviation of 21.2. Our series included 50 adenomyomatous hyperplasias, 7 adenomyomatous hyperplasias associated with chronic prostatitis, 2 HGPIN and 1 LGPIN. After re-reading we found 5 discordant cases, which corresponded to minimal suspect foci (kappa = 0.5098). The p63 marking was informative in 53 cases, i.e. 88%, and non-informative in 7 cases, i.e. 12%. Among the uninformative markings, 2 were due to lack of tissue adhesion to the slides. Among the informative markings, 11 were negative. p63 immunohistochemistry was useful in all suspected foci and detected 6 other minimal foci of adenocarcinoma. Conclusion: The immunostaining with the anti-p63 antibody in the prostate cancer diagnosis is of considerable benefit. It made it possible to correct 11.3% of benign diagnosis in minimal malignant focus in our context. Despite the difficulties associated with the manual technique, it is possible to have an informative rate, similar to the automatic technique.展开更多
自从淋巴细胞杂交瘤1975年创立以来(Kohler et al.,1975)越来越显示其优越性,(Sikora et al.,1982),从其发展看来,前景广阔,针对恶性肿瘤,白血病和组织移植等提出了崭新的疗法。由于鼠源性单克隆抗体在临床应用上往往引起过敏反应和形...自从淋巴细胞杂交瘤1975年创立以来(Kohler et al.,1975)越来越显示其优越性,(Sikora et al.,1982),从其发展看来,前景广阔,针对恶性肿瘤,白血病和组织移植等提出了崭新的疗法。由于鼠源性单克隆抗体在临床应用上往往引起过敏反应和形成免疫复合物的危险性,激励着科学家们去开拓人—人杂交瘤,尽管前进的道路上还存在着许多困难,但人们还是不屈不挠地前进。目前,世界上共有三个人—人杂交瘤系统。(1)0lsson和Kaplan等培育的SKO—007人骨髓瘤细胞系统。(2)Croce等培育的GM 1500细胞系统。(3)Clark等的RPMI8226细胞系统。并且都进行了人—人杂交瘤的研究,但是多数杂交瘤阳性率低和分泌抗体量少。国内北京、上海、西安和昆明等都正在开展本项研究。我们从1984年8月,开始人单抗的研究,也碰到许多困难,深深体会到建立一个具有我国特色的人—人杂交瘤系统是十分重要的,现将我们的工作简要报告如下。展开更多
目的了解广西壮族自治区（广西）4个县0~15岁人群的肠道病毒71型（Enterovirus Type 71,EV71）中和抗体（Neutralizing Antibody,NA）水平,为制定EV71疫苗免疫程序提供参考。方法选取广西4个县作为研究现场,在每个县选取2个乡,对调查现...目的了解广西壮族自治区（广西）4个县0~15岁人群的肠道病毒71型（Enterovirus Type 71,EV71）中和抗体（Neutralizing Antibody,NA）水平,为制定EV71疫苗免疫程序提供参考。方法选取广西4个县作为研究现场,在每个县选取2个乡,对调查现场的0~15岁人群采集静脉血标本。结果≤6月龄各月龄组研究对象血清EV71 NA阳性率随年龄的增长而迅速下降;直到9~11月龄仍维持在较低水平（20.0%）,≥1岁各年龄组NA阳性率迅速上升,其中5岁组的EV71 NA阳性率最高（87.0%）。0~15岁人群的EV71 NA几何平均滴度（Geometric Mean Titer,GMT）为1∶18.24,新生儿EV71 NA GMT较高（1∶23.12）,随着月龄增长,EV71 NA GMT逐渐下降,至5月龄降至最低（1∶5.46）,≥1岁各年龄组的NA GMT迅速上升,5岁组最高（1∶95.68）。4个县及不同性别的分年龄组EV71 NA阳性率及GMT与EV71总的NA阳性率及GMT相似。结论 5~6月龄婴儿EV71 NA阳性率及GMT均降至最低水平,为确定EV71疫苗接种起始月龄提供参考。展开更多
目的了解六个县0～15岁人群柯萨奇病毒A组16型(Coxsackievirus Group A Type16,CVA16)中和抗体(Neutralizing Antibody,NA)水平。方法选取广西壮族自治区和江苏省的3个手足口病(Hand,Foot and MouthDisease,HFMD)高发县和3个HFMD低发县...目的了解六个县0～15岁人群柯萨奇病毒A组16型(Coxsackievirus Group A Type16,CVA16)中和抗体(Neutralizing Antibody,NA)水平。方法选取广西壮族自治区和江苏省的3个手足口病(Hand,Foot and MouthDisease,HFMD)高发县和3个HFMD低发县作为研究现场,在每个县选取一个HFMD高发乡和一个HFMD低发乡,对调查现场的0～15岁人群及新生儿母亲采集静脉血标本,并对他们(或其家长)进行问卷调查。结果 <1岁各月龄组调查对象的CVA16血清NA阳性率随年龄的增长而下降;≥1岁各年龄组NA阳性率逐渐上升,其中6～10岁的CVA16NA阳性率最高。不同年龄、不同县调查对象CVA_(16)NA的阳性率差异均有统计学意义,但男、女性CVA_(16)NA的阳性率差异无统计学意义。0～15岁人群的CVA_(16)NA几何平均滴度(Geometric Mean Titer,GMT)为1∶8.44,<1岁各月龄和1岁的NA GMT均<1∶10,>1岁各年龄组NA的GMT上升,最高至1∶22.97。各县及不同性别的分年龄组NA GMT曲线与总NA的GMT曲线相似。结论六个县调查对象的CVA_(16)NA阳性率及GMT均较低,该六县0～15岁人群有发生CVA_(16)引起的HFMD爆发的风险。展开更多
目的制备甲型副伤寒沙门菌菌体抗原单克隆抗体,并对其进行鉴定及初步应用。方法用甲醛灭活的甲型副伤寒沙门菌免疫BALB/c小鼠,取脾细胞与Sp2/0骨髓瘤细胞融合,筛选出稳定分泌甲型副伤寒沙门菌特异性单克隆抗体的杂交瘤细胞株,对克隆纯...目的制备甲型副伤寒沙门菌菌体抗原单克隆抗体,并对其进行鉴定及初步应用。方法用甲醛灭活的甲型副伤寒沙门菌免疫BALB/c小鼠,取脾细胞与Sp2/0骨髓瘤细胞融合,筛选出稳定分泌甲型副伤寒沙门菌特异性单克隆抗体的杂交瘤细胞株,对克隆纯化后的阳性细胞扩大培养后,免疫BALB/c小鼠,收获腹水,间接ELISA法检测抗体效价,并进行Ig类和亚类鉴定;腹水经辛酸-硫酸铵盐析法纯化后,采用间接ELISA法、玻片凝集试验、SDSPAGE法、免疫扩散试验进行检测。将纯化后的2株单克隆抗体用于双抗体夹心ELISA法的建立,并用自制配对抗体检测甲型副伤寒患者血浆、乙型副伤寒患者血浆、伤寒患者血浆和正常人血浆。结果共筛选出2株持续分泌单克隆抗体的杂交瘤细胞株,命名为1H4和2A9,腹水抗体效价为1∶10~7和1∶10~6,分别为IgG_1和Ig G_3亚类,轻链均为κ型。纯化后的单克隆抗体1H4、2A9效价分别为1∶10~7和1∶10~3,1H4纯度大于85%,2A9纯度小于60%。2株单克隆抗体与甲型副伤寒沙门菌50503、50973可产生凝集反应,与伤寒沙门菌50096不产生反应;1H4在稀释度为1∶8时可与甲型副伤寒沙门菌菌体特异多糖(organism specific polysaccharide,OSP)产生凝集反应,而2A9在每个稀释度与OSP均不发生反应。将1H4和2A9用于双抗体夹心ELISA检测法建立,确定1H4/HRP-2A9的组合为最佳配对,用该配对抗体检测阳性样本检出率为100%,正常人血浆样本检出率为0。结论获得2株甲型副伤寒沙门菌菌体抗原单克隆抗体,可用于甲型副伤寒沙门菌的快速鉴定。展开更多
文摘Introduction: Prostate cancer is the second most common cancer in men. The diagnosis is most often based on the prostate biopsies’ analysis and on histological criteria recognizable on standard coloring. In some cases, the use of immunohistochemistry is important. Objectives: This paper aims to specify the p63 phenotypic profile of lesions diagnosed benign, with minimal suspect foci, difficult to interpret, HGPIN (high grade intraepithelial neoplasia) and LGPIN (low-grade prostatic intraepithelial neoplasia) and evaluate the manual technique of p63 immunohistochemistry. Patients and Method: This was a retrospective, descriptive study of prostate biopsies recorded in the PAC service of the HALD from January 1st, 2018 to December 31st, 2018. It was completed by a manual immunohistochemical study of the blocks enrolled from November 19th to December 4th, 2020 in the PAC department of the HPD. The studied parameters were: registry number, age, clinical stage, prostate volume, PSA level, microscopic appearance and p63 immunohistochemical profile. Results: Our study included 60 prostate biopsies. The ages of our patients varied from 45 to 77 years, with an average of 64.2 years and a standard deviation of 6.2. The majority of patients were at clinical stage cT2b (33%) with a prostate volume varying between 33.15 and 169.4 cc. The minimum value of PSA in our series is 5 ng/ml, the maximum being 100 ng/ml with an average level of 24.1 ng/ml and a standard deviation of 21.2. Our series included 50 adenomyomatous hyperplasias, 7 adenomyomatous hyperplasias associated with chronic prostatitis, 2 HGPIN and 1 LGPIN. After re-reading we found 5 discordant cases, which corresponded to minimal suspect foci (kappa = 0.5098). The p63 marking was informative in 53 cases, i.e. 88%, and non-informative in 7 cases, i.e. 12%. Among the uninformative markings, 2 were due to lack of tissue adhesion to the slides. Among the informative markings, 11 were negative. p63 immunohistochemistry was useful in all suspected foci and detected 6 other minimal foci of adenocarcinoma. Conclusion: The immunostaining with the anti-p63 antibody in the prostate cancer diagnosis is of considerable benefit. It made it possible to correct 11.3% of benign diagnosis in minimal malignant focus in our context. Despite the difficulties associated with the manual technique, it is possible to have an informative rate, similar to the automatic technique.
文摘自从淋巴细胞杂交瘤1975年创立以来(Kohler et al.,1975)越来越显示其优越性,(Sikora et al.,1982),从其发展看来,前景广阔,针对恶性肿瘤,白血病和组织移植等提出了崭新的疗法。由于鼠源性单克隆抗体在临床应用上往往引起过敏反应和形成免疫复合物的危险性,激励着科学家们去开拓人—人杂交瘤,尽管前进的道路上还存在着许多困难,但人们还是不屈不挠地前进。目前,世界上共有三个人—人杂交瘤系统。(1)0lsson和Kaplan等培育的SKO—007人骨髓瘤细胞系统。(2)Croce等培育的GM 1500细胞系统。(3)Clark等的RPMI8226细胞系统。并且都进行了人—人杂交瘤的研究,但是多数杂交瘤阳性率低和分泌抗体量少。国内北京、上海、西安和昆明等都正在开展本项研究。我们从1984年8月,开始人单抗的研究,也碰到许多困难,深深体会到建立一个具有我国特色的人—人杂交瘤系统是十分重要的,现将我们的工作简要报告如下。
文摘目的了解广西壮族自治区（广西）4个县0~15岁人群的肠道病毒71型（Enterovirus Type 71,EV71）中和抗体（Neutralizing Antibody,NA）水平,为制定EV71疫苗免疫程序提供参考。方法选取广西4个县作为研究现场,在每个县选取2个乡,对调查现场的0~15岁人群采集静脉血标本。结果≤6月龄各月龄组研究对象血清EV71 NA阳性率随年龄的增长而迅速下降;直到9~11月龄仍维持在较低水平（20.0%）,≥1岁各年龄组NA阳性率迅速上升,其中5岁组的EV71 NA阳性率最高（87.0%）。0~15岁人群的EV71 NA几何平均滴度（Geometric Mean Titer,GMT）为1∶18.24,新生儿EV71 NA GMT较高（1∶23.12）,随着月龄增长,EV71 NA GMT逐渐下降,至5月龄降至最低（1∶5.46）,≥1岁各年龄组的NA GMT迅速上升,5岁组最高（1∶95.68）。4个县及不同性别的分年龄组EV71 NA阳性率及GMT与EV71总的NA阳性率及GMT相似。结论 5~6月龄婴儿EV71 NA阳性率及GMT均降至最低水平,为确定EV71疫苗接种起始月龄提供参考。
文摘目的了解六个县0～15岁人群柯萨奇病毒A组16型(Coxsackievirus Group A Type16,CVA16)中和抗体(Neutralizing Antibody,NA)水平。方法选取广西壮族自治区和江苏省的3个手足口病(Hand,Foot and MouthDisease,HFMD)高发县和3个HFMD低发县作为研究现场,在每个县选取一个HFMD高发乡和一个HFMD低发乡,对调查现场的0～15岁人群及新生儿母亲采集静脉血标本,并对他们(或其家长)进行问卷调查。结果 <1岁各月龄组调查对象的CVA16血清NA阳性率随年龄的增长而下降;≥1岁各年龄组NA阳性率逐渐上升,其中6～10岁的CVA16NA阳性率最高。不同年龄、不同县调查对象CVA_(16)NA的阳性率差异均有统计学意义,但男、女性CVA_(16)NA的阳性率差异无统计学意义。0～15岁人群的CVA_(16)NA几何平均滴度(Geometric Mean Titer,GMT)为1∶8.44,<1岁各月龄和1岁的NA GMT均<1∶10,>1岁各年龄组NA的GMT上升,最高至1∶22.97。各县及不同性别的分年龄组NA GMT曲线与总NA的GMT曲线相似。结论六个县调查对象的CVA_(16)NA阳性率及GMT均较低,该六县0～15岁人群有发生CVA_(16)引起的HFMD爆发的风险。
文摘目的制备甲型副伤寒沙门菌菌体抗原单克隆抗体,并对其进行鉴定及初步应用。方法用甲醛灭活的甲型副伤寒沙门菌免疫BALB/c小鼠,取脾细胞与Sp2/0骨髓瘤细胞融合,筛选出稳定分泌甲型副伤寒沙门菌特异性单克隆抗体的杂交瘤细胞株,对克隆纯化后的阳性细胞扩大培养后,免疫BALB/c小鼠,收获腹水,间接ELISA法检测抗体效价,并进行Ig类和亚类鉴定;腹水经辛酸-硫酸铵盐析法纯化后,采用间接ELISA法、玻片凝集试验、SDSPAGE法、免疫扩散试验进行检测。将纯化后的2株单克隆抗体用于双抗体夹心ELISA法的建立,并用自制配对抗体检测甲型副伤寒患者血浆、乙型副伤寒患者血浆、伤寒患者血浆和正常人血浆。结果共筛选出2株持续分泌单克隆抗体的杂交瘤细胞株,命名为1H4和2A9,腹水抗体效价为1∶10~7和1∶10~6,分别为IgG_1和Ig G_3亚类,轻链均为κ型。纯化后的单克隆抗体1H4、2A9效价分别为1∶10~7和1∶10~3,1H4纯度大于85%,2A9纯度小于60%。2株单克隆抗体与甲型副伤寒沙门菌50503、50973可产生凝集反应,与伤寒沙门菌50096不产生反应;1H4在稀释度为1∶8时可与甲型副伤寒沙门菌菌体特异多糖(organism specific polysaccharide,OSP)产生凝集反应,而2A9在每个稀释度与OSP均不发生反应。将1H4和2A9用于双抗体夹心ELISA检测法建立,确定1H4/HRP-2A9的组合为最佳配对,用该配对抗体检测阳性样本检出率为100%,正常人血浆样本检出率为0。结论获得2株甲型副伤寒沙门菌菌体抗原单克隆抗体,可用于甲型副伤寒沙门菌的快速鉴定。
