The development of natural biomaterials applied for hard tissue repair and regeneration is of great importance,especially in societies with a large elderly population.Self-assembled peptide hydrogels are a new generat...The development of natural biomaterials applied for hard tissue repair and regeneration is of great importance,especially in societies with a large elderly population.Self-assembled peptide hydrogels are a new generation of biomaterials that provide excellent biocompatibility,tunable mechanical stability,injectability,trigger capability,lack of immunogenic reactions,and the ability to load cells and active pharmaceutical agents for tissue regeneration.Peptide-based hydrogels are ideal templates for the deposition of hydroxyapatite crystals,which can mimic the extracellular matrix.Thus,peptide-based hydrogels enhance hard tissue repair and regeneration compared to conventional methods.This review presents three major self-assembled peptide hydrogels with potential application for bone and dental tissue regeneration,including ionic self-complementary peptides,amphiphilic(surfactant-like)peptides,and triple-helix(collagen-like)peptides.Special attention is given to the main bioactive peptides,the role and importance of self-assembled peptide hydrogels,and a brief overview on molecular simulation of self-assembled peptide hydrogels applied for bone and dental tissue engineering and regeneration.展开更多
Nanocellulose(NC) has attracted much interest in the tissue engineering(TE) field because of its properties including biocompatibility,renewability, non-toxicity, functionality, and excellent mechanical performance. T...Nanocellulose(NC) has attracted much interest in the tissue engineering(TE) field because of its properties including biocompatibility,renewability, non-toxicity, functionality, and excellent mechanical performance. This review mainly focused on the advanced applications of NC-based composites in hard TE including cartilage TE, bone TE, and dental TE, illustrated the processing methods for synthesizing scaffolds including electrospinning, freeze-drying, and 3 D printing, reviewed the current status of hard TE, and presented perspective on the future of TE technology.展开更多
This study deals with an overview of the biomedical projects performed at iThemba LABS, particularly in relation to the spatial distribution of trace metals in hard human tissues from disadvantaged Sudanese communitie...This study deals with an overview of the biomedical projects performed at iThemba LABS, particularly in relation to the spatial distribution of trace metals in hard human tissues from disadvantaged Sudanese communities, such as kidney stone concretions, teeth and hair, undertaken at the iThemba LABS Nuclear Microprobe (NMP) facility. Relevant information about the ion beam techniques used for material characterization was discussed. The Particle Induced X-ray Emission (PIXE) technique was used to investigate the elemental composition of human hard tissues on a microscopic scale with the elemental mapping;complementary ion beam techniques are used to provide information on the major and minor components. Analysis of teeth sections by μ-PIXE showed that the levels of trace elements were enriched and/or depleted, this may associated with leaching and/or erosive processes. On the other hand the characterization of kidney stone concretions by μ-PIXE showed a marked difference of individuals from different region within Sudan.展开更多
Mineralization has found widespread use in the fabrication of composite biomaterials for hard tissue regeneration.The current mineralization processes are mainly carried out in neutral aqueous solutions of biomineral ...Mineralization has found widespread use in the fabrication of composite biomaterials for hard tissue regeneration.The current mineralization processes are mainly carried out in neutral aqueous solutions of biomineral counter-ions(a pair of cation and anion that form the corresponding minerals at certain conditions),which are stable only at very low concentrations.This typically results in inefficient mineralization and weak control over biomineral formation.Here,we find that,in the organic solvent glycerol,a variety of biomineral counter-ions(e.g.,Ca/PO_(4),Ca/CO_(3),Ca/SO_(4),Mg/PO_(4),or Fe/OH)corresponding to distinct biominerals at significantly high concentrations(up to hundreds-fold greater than those of simulated body fluid(SBF))are able to form translucent and stable solutions(mineralizing solution of highly concentrated counter-ions(MSCIs)),and mineralization can be triggered upon them with external solvents(e.g.,water or ethanol).Furthermore,with pristine bacterial cellulose(BC)membrane as a model,we demonstrate an effective and controllable mineralization performance of MSCIs on organic substrates.This approach not only forms the homogeneous biominerals on the BC fibers and in the interspaces,but also provides regulations over mineralization rate,mineral content,phase,and dopants.The resulting mineralized BC membranes(MBCs)exhibit high cytocompatibility and favor the proliferation of rat bone marrow mesenchymal stem cells(rBMSC).Following this,we prepare a mineralized bone suture(MBS)from MBC for non-weight bearing bone fixation,which then is tested on a rabbit median sternotomy model.It shows firm fixation of the rabbit sternum without causing discernible toxicity or inflammatory response.This study,by extending the mineralization to the organic solution system of highly concentrated counter-ions,develops a promising strategy to design and build targeted mineral-based composites.展开更多
Three-dimensional printing technology with the rapid development of printing materials are widely recognized as a promising way to fabricate bioartificial bone tissues.In consideration of the disadvantages of bone sub...Three-dimensional printing technology with the rapid development of printing materials are widely recognized as a promising way to fabricate bioartificial bone tissues.In consideration of the disadvantages of bone substitutes,including poor mechanical properties,lack of vascularization and insufficient osteointegration,functional modification strategies can provide multiple functions and desired characteristics of printing materials,enhance their physicochemical and biological properties in bone tissue engineering.Thus,this review focuses on the advances of functional engineering strategies for 3D printed biomaterials in hard tissue replacement.It is structured as introducing 3D printing technologies,properties of printing materials(metals,ceramics and polymers)and typical functional engineering strategies utilized in the application of bone,cartilage and joint regeneration.展开更多
文摘The development of natural biomaterials applied for hard tissue repair and regeneration is of great importance,especially in societies with a large elderly population.Self-assembled peptide hydrogels are a new generation of biomaterials that provide excellent biocompatibility,tunable mechanical stability,injectability,trigger capability,lack of immunogenic reactions,and the ability to load cells and active pharmaceutical agents for tissue regeneration.Peptide-based hydrogels are ideal templates for the deposition of hydroxyapatite crystals,which can mimic the extracellular matrix.Thus,peptide-based hydrogels enhance hard tissue repair and regeneration compared to conventional methods.This review presents three major self-assembled peptide hydrogels with potential application for bone and dental tissue regeneration,including ionic self-complementary peptides,amphiphilic(surfactant-like)peptides,and triple-helix(collagen-like)peptides.Special attention is given to the main bioactive peptides,the role and importance of self-assembled peptide hydrogels,and a brief overview on molecular simulation of self-assembled peptide hydrogels applied for bone and dental tissue engineering and regeneration.
基金the special fund for Independent Innovation and Industry Development in the Core Area in Haidian District of Beijing (255-kjc020)
文摘Nanocellulose(NC) has attracted much interest in the tissue engineering(TE) field because of its properties including biocompatibility,renewability, non-toxicity, functionality, and excellent mechanical performance. This review mainly focused on the advanced applications of NC-based composites in hard TE including cartilage TE, bone TE, and dental TE, illustrated the processing methods for synthesizing scaffolds including electrospinning, freeze-drying, and 3 D printing, reviewed the current status of hard TE, and presented perspective on the future of TE technology.
文摘This study deals with an overview of the biomedical projects performed at iThemba LABS, particularly in relation to the spatial distribution of trace metals in hard human tissues from disadvantaged Sudanese communities, such as kidney stone concretions, teeth and hair, undertaken at the iThemba LABS Nuclear Microprobe (NMP) facility. Relevant information about the ion beam techniques used for material characterization was discussed. The Particle Induced X-ray Emission (PIXE) technique was used to investigate the elemental composition of human hard tissues on a microscopic scale with the elemental mapping;complementary ion beam techniques are used to provide information on the major and minor components. Analysis of teeth sections by μ-PIXE showed that the levels of trace elements were enriched and/or depleted, this may associated with leaching and/or erosive processes. On the other hand the characterization of kidney stone concretions by μ-PIXE showed a marked difference of individuals from different region within Sudan.
基金supported by the National Key R&D Program of China(No.2022YFE0123500)the National Natural Science Foundation of China(Nos.52272304 and 31771081)Science and Technology Commission of Shanghai Municipality(Nos.21ZR1449700,22S31903300,and 22S31900100).
文摘Mineralization has found widespread use in the fabrication of composite biomaterials for hard tissue regeneration.The current mineralization processes are mainly carried out in neutral aqueous solutions of biomineral counter-ions(a pair of cation and anion that form the corresponding minerals at certain conditions),which are stable only at very low concentrations.This typically results in inefficient mineralization and weak control over biomineral formation.Here,we find that,in the organic solvent glycerol,a variety of biomineral counter-ions(e.g.,Ca/PO_(4),Ca/CO_(3),Ca/SO_(4),Mg/PO_(4),or Fe/OH)corresponding to distinct biominerals at significantly high concentrations(up to hundreds-fold greater than those of simulated body fluid(SBF))are able to form translucent and stable solutions(mineralizing solution of highly concentrated counter-ions(MSCIs)),and mineralization can be triggered upon them with external solvents(e.g.,water or ethanol).Furthermore,with pristine bacterial cellulose(BC)membrane as a model,we demonstrate an effective and controllable mineralization performance of MSCIs on organic substrates.This approach not only forms the homogeneous biominerals on the BC fibers and in the interspaces,but also provides regulations over mineralization rate,mineral content,phase,and dopants.The resulting mineralized BC membranes(MBCs)exhibit high cytocompatibility and favor the proliferation of rat bone marrow mesenchymal stem cells(rBMSC).Following this,we prepare a mineralized bone suture(MBS)from MBC for non-weight bearing bone fixation,which then is tested on a rabbit median sternotomy model.It shows firm fixation of the rabbit sternum without causing discernible toxicity or inflammatory response.This study,by extending the mineralization to the organic solution system of highly concentrated counter-ions,develops a promising strategy to design and build targeted mineral-based composites.
基金supported partly by the Zhejiang Provincial Natural Science Foundation of China(LY20E010006)partly by the National Natural Science Foundation of China(51502265 and 81701033).
文摘Three-dimensional printing technology with the rapid development of printing materials are widely recognized as a promising way to fabricate bioartificial bone tissues.In consideration of the disadvantages of bone substitutes,including poor mechanical properties,lack of vascularization and insufficient osteointegration,functional modification strategies can provide multiple functions and desired characteristics of printing materials,enhance their physicochemical and biological properties in bone tissue engineering.Thus,this review focuses on the advances of functional engineering strategies for 3D printed biomaterials in hard tissue replacement.It is structured as introducing 3D printing technologies,properties of printing materials(metals,ceramics and polymers)and typical functional engineering strategies utilized in the application of bone,cartilage and joint regeneration.
