The diseases of sweet cherry caused by viruses and viroids have occurred in the main cherry producing areas of China and are increasing year by year, which has become an important factor affecting the yield and qualit...The diseases of sweet cherry caused by viruses and viroids have occurred in the main cherry producing areas of China and are increasing year by year, which has become an important factor affecting the yield and quality of sweet cherry. For this reason, this paper elaborated the prevention and countermeasures of virus diseases from six aspects, including soil treatment, cultivation of disease-free seedlings, prevention of disease and pest damage, reasonable pruning, water management, and treatment of virus diseases, in order to provide technical guidance for the disease resistance, high quality and high yield of facility cherry, as well as information reference for the further improvement of the technical system of virus disease prevention and cultivation of facility cherry.展开更多
There are a total of more than 40 reported maize viral diseases worldwide. Five of them have reportedly occurred in China. They are maize rough dwarf disease, maize dwarf mosaic disease, maize streak dwarf disease, ma...There are a total of more than 40 reported maize viral diseases worldwide. Five of them have reportedly occurred in China. They are maize rough dwarf disease, maize dwarf mosaic disease, maize streak dwarf disease, maize crimson leaf disease, maize wallaby ear disease and corn lethal necrosis disease. This paper reviewed their occurrence and distribution as well as virus identification techniques in order to provide a basis for virus identification and diagnosis in corn production.展开更多
Viral hepatitis B and C virus(HBV and HCV) are responsible for the most of chronic liver disease worldwide and are transmitted by parenteral route, sexual and vertical transmission. One important measure to reduce the...Viral hepatitis B and C virus(HBV and HCV) are responsible for the most of chronic liver disease worldwide and are transmitted by parenteral route, sexual and vertical transmission. One important measure to reduce the burden of these infections is the diagnosis of acute and chronic cases of HBV and HCV. In order to provide an effective diagnosis and monitoring of antiviral treatment, it is important to choose sensitive, rapid, inexpensive, and robust analytical methods. Primary diagnosis of HBV and HCV infection is made by using serological tests for detecting antigens and antibodies against these viruses. In order to confirm primary diagnosis, to quantify viral load, to determine genotypes and resistance mutants for antiviral treatment, qualitative and quantitative molecular tests are used. In this manuscript, we review the current serological and molecular methods for the diagnosis of hepatitis B and C.展开更多
We are presenting the numerical analysis for stochastic SLBR model of computer virus over the internet in this manuscript.We are going to present the results of stochastic and deterministic computer virus model.Outcom...We are presenting the numerical analysis for stochastic SLBR model of computer virus over the internet in this manuscript.We are going to present the results of stochastic and deterministic computer virus model.Outcomes of the threshold number C∗hold in stochastic computer virus model.If C∗<1 then in such a condition virus controlled in the computer population while C∗>1 shows virus spread in the computer population.Unfortunately,stochastic numerical techniques fail to cope with large step sizes of time.The suggested structure of the stochastic non-standard finite difference scheme(SNSFD)maintains all diverse characteristics such as dynamical consistency,bounded-ness and positivity as well-defined by Mickens.On this basis,we can suggest a collection of plans for eradicating viruses spreading across the internet effectively.展开更多
We are presenting the numerical simulations for the stochastic computer virus propagation model in this manuscript.We are comparing the solutions of stochastic and deterministic computer virus models.Outcomes of a thr...We are presenting the numerical simulations for the stochastic computer virus propagation model in this manuscript.We are comparing the solutions of stochastic and deterministic computer virus models.Outcomes of a threshold number R0 hold in stochastic computer virus model.If R_(0)<1 then in such a condition virus controlled in the computer population while R_(0)>1 shows virus rapidly spread in the computer population.Unfortunately,stochastic numerical techniques fail to cope with large step sizes of time.The suggested structure of the stochastic non-standard finite difference technique can never violate the dynamical properties.On this basis,we can suggest a collection of strategies for removing virus’s propagation in the computer population.展开更多
INTRODUCTIONHepatitis B viral infection, one of the most-prevalent liver disorders in China and Korea, is aserious infectious disease as it has the potential ofprogressing into liver cirrhosis and primary hepaticcarci...INTRODUCTIONHepatitis B viral infection, one of the most-prevalent liver disorders in China and Korea, is aserious infectious disease as it has the potential ofprogressing into liver cirrhosis and primary hepaticcarcinoma. China and Korea both belong to high-risk endemic regions of viral hepatitis[1]. TheHBsAg positive rates in China ranged from 6.9% -17.9% by age, race and test methods[2-5].展开更多
Porcine reproductive and respiratory syndrome virus(PRRSV)GP4 protein was prokaryotically expressed,and used as an antigen to immunize six-week-old BALB/c female mice.With conventional cell fusion method,an anti-PRRSV...Porcine reproductive and respiratory syndrome virus(PRRSV)GP4 protein was prokaryotically expressed,and used as an antigen to immunize six-week-old BALB/c female mice.With conventional cell fusion method,an anti-PRRSV GP4 protein monoclonal antibody(Mab)5F12 was successfully prepared.It was identified as IgG2b subclass and had better stability and specificity,which not only responded with recombinant PRRSV GP4 protein,but also with PRRSV.Phage display technique had varieties of applications,in particular,the identification of key antigen epitopes for the development of therapeutic and diagnostic reagents and vaccines.In this study,Mab-5F12 was used as the target for biopanning a 12-mer phage random peptide library.After four rounds of biopanning,two phage-displayed peptides,named P-A and P-G(AKFEVCSPVVLG and GVNQENMLHFSF)were identified that recognized Mab-5F12 specifically.Sequence analysis showed that one or more of the peptides exhibited partial sequence similarity to the native GP4 protein sequence,which corresponded to 69-80 and 84-95 aa segments of the HP-PRRSV GP4 protein.Furthermore,real-time quantitative RT-PCR and indirect immunofluorescence assay indicated consistently the abilities of P-A and P-G to block viral infection in Marc-145 cells and they could function as antiviral agents for PRRSV.展开更多
基金Supported by Class A Project of Scientific Research Development Program of Colleges and Universities in Shandong Province (J17KA155)。
文摘The diseases of sweet cherry caused by viruses and viroids have occurred in the main cherry producing areas of China and are increasing year by year, which has become an important factor affecting the yield and quality of sweet cherry. For this reason, this paper elaborated the prevention and countermeasures of virus diseases from six aspects, including soil treatment, cultivation of disease-free seedlings, prevention of disease and pest damage, reasonable pruning, water management, and treatment of virus diseases, in order to provide technical guidance for the disease resistance, high quality and high yield of facility cherry, as well as information reference for the further improvement of the technical system of virus disease prevention and cultivation of facility cherry.
基金Supported by the Finance Department of Hebei Province(A2012120104)
文摘There are a total of more than 40 reported maize viral diseases worldwide. Five of them have reportedly occurred in China. They are maize rough dwarf disease, maize dwarf mosaic disease, maize streak dwarf disease, maize crimson leaf disease, maize wallaby ear disease and corn lethal necrosis disease. This paper reviewed their occurrence and distribution as well as virus identification techniques in order to provide a basis for virus identification and diagnosis in corn production.
文摘Viral hepatitis B and C virus(HBV and HCV) are responsible for the most of chronic liver disease worldwide and are transmitted by parenteral route, sexual and vertical transmission. One important measure to reduce the burden of these infections is the diagnosis of acute and chronic cases of HBV and HCV. In order to provide an effective diagnosis and monitoring of antiviral treatment, it is important to choose sensitive, rapid, inexpensive, and robust analytical methods. Primary diagnosis of HBV and HCV infection is made by using serological tests for detecting antigens and antibodies against these viruses. In order to confirm primary diagnosis, to quantify viral load, to determine genotypes and resistance mutants for antiviral treatment, qualitative and quantitative molecular tests are used. In this manuscript, we review the current serological and molecular methods for the diagnosis of hepatitis B and C.
基金Prince Sultan University for funding this work through research-group number RG-DES2017-01-17.
文摘We are presenting the numerical analysis for stochastic SLBR model of computer virus over the internet in this manuscript.We are going to present the results of stochastic and deterministic computer virus model.Outcomes of the threshold number C∗hold in stochastic computer virus model.If C∗<1 then in such a condition virus controlled in the computer population while C∗>1 shows virus spread in the computer population.Unfortunately,stochastic numerical techniques fail to cope with large step sizes of time.The suggested structure of the stochastic non-standard finite difference scheme(SNSFD)maintains all diverse characteristics such as dynamical consistency,bounded-ness and positivity as well-defined by Mickens.On this basis,we can suggest a collection of plans for eradicating viruses spreading across the internet effectively.
文摘We are presenting the numerical simulations for the stochastic computer virus propagation model in this manuscript.We are comparing the solutions of stochastic and deterministic computer virus models.Outcomes of a threshold number R0 hold in stochastic computer virus model.If R_(0)<1 then in such a condition virus controlled in the computer population while R_(0)>1 shows virus rapidly spread in the computer population.Unfortunately,stochastic numerical techniques fail to cope with large step sizes of time.The suggested structure of the stochastic non-standard finite difference technique can never violate the dynamical properties.On this basis,we can suggest a collection of strategies for removing virus’s propagation in the computer population.
基金Project supported by the National Natural Science Foundation of China, No. 39560074Korea ScienceEngineering Foundation, 965-0700-001-2.
文摘INTRODUCTIONHepatitis B viral infection, one of the most-prevalent liver disorders in China and Korea, is aserious infectious disease as it has the potential ofprogressing into liver cirrhosis and primary hepaticcarcinoma. China and Korea both belong to high-risk endemic regions of viral hepatitis[1]. TheHBsAg positive rates in China ranged from 6.9% -17.9% by age, race and test methods[2-5].
基金Supported by the National Natural Science Foundation of China(31372438,31200122)
文摘Porcine reproductive and respiratory syndrome virus(PRRSV)GP4 protein was prokaryotically expressed,and used as an antigen to immunize six-week-old BALB/c female mice.With conventional cell fusion method,an anti-PRRSV GP4 protein monoclonal antibody(Mab)5F12 was successfully prepared.It was identified as IgG2b subclass and had better stability and specificity,which not only responded with recombinant PRRSV GP4 protein,but also with PRRSV.Phage display technique had varieties of applications,in particular,the identification of key antigen epitopes for the development of therapeutic and diagnostic reagents and vaccines.In this study,Mab-5F12 was used as the target for biopanning a 12-mer phage random peptide library.After four rounds of biopanning,two phage-displayed peptides,named P-A and P-G(AKFEVCSPVVLG and GVNQENMLHFSF)were identified that recognized Mab-5F12 specifically.Sequence analysis showed that one or more of the peptides exhibited partial sequence similarity to the native GP4 protein sequence,which corresponded to 69-80 and 84-95 aa segments of the HP-PRRSV GP4 protein.Furthermore,real-time quantitative RT-PCR and indirect immunofluorescence assay indicated consistently the abilities of P-A and P-G to block viral infection in Marc-145 cells and they could function as antiviral agents for PRRSV.
