不同品系小鼠异种被动皮肤过敏试验反应耳廓蓝染与血液抗体IgE和IgG水平的研究

目的观察卵白蛋白(OVA)致大鼠过敏抗血清对不同品系小鼠被动皮肤过敏试验(PCA)及血液免疫球蛋白E(IgE)和免疫球蛋白G(IgG)抗体水平变化的影响。方法 Wistar大鼠随机分为生理盐水(NS)组、NS佐剂组、OVA组、OVA佐剂组,采用皮下注射方式给予相应药物致敏,制备大鼠OVA抗体血清,并分别将其注入不同品系小鼠耳廓内,于致敏后3 h或48 h以相应药物激发,观察各组小鼠耳廓蓝染情况,并用酶联免疫吸附剂测定(ELISA)检测小鼠体内抗体水平变化。结果各品系小鼠(KM、BALB/c、ICR、C57BL/6、B6;129S-Fcgr2btm1Ttk/J)NS组、NS佐剂组、OVA组耳廓蓝染阴性,OVA佐剂组耳廓蓝染阳性,其中各品系间存在差异,小鼠耳异种PCA敏感程度强度依次为KM、BALB/c>ICR、C57BL/6、B6;129S-Fcgr2btm1Ttk/J。结论小鼠异种PCA试验中,不同品系动物对OVA抗血清的敏感性存在差异,其中KM,BALB/c小鼠较为敏感,但PCA强度与血液IgE水平无明显的相关性。

A、B、AB型全血加入O型全血37℃对血型鉴定、抗体效价及抗球蛋白试验的影响

目的了解A、B、AB型全血加入O型全血不同剂量后血型正反鉴定、抗-A、抗-B抗体效价、直接与间接抗人-IgG实验变化。方法完全随机抽取4℃保存24 h A、B、AB型全血分别60 m l,按不同比例加入O型抗-A、抗-B效价1∶64的全血9、12、15、18 m l置入100 m l塑料血袋内混匀后,置37℃孵箱,间隔15 m in摇动1次。分别在1、2、4、8、12、24 h留取标本,做血型正反鉴定、抗-A、抗-B抗体效价、直接与间接抗人-IgG实验。结果不同型全血加入不同剂量O型全血,37℃不同时间血型正反鉴定相符;相对应抗-A、抗-B抗体效价逐渐下降到消失;直接与间接抗人-IgG实验的变化,以B型接受O型的反应最小,在接受600 m l直接与间接抗人-IgG均为阴性,800 m l同1 200 m l反应基本一致。A、AB型接受O型后直接与间接抗人-IgG反应基本一致,实验结果均为阳性。结论以B型接受O型的反应最小,在接受600 m l直接与间接抗人-IgG均为阴性。800 m l同1 200 m l反应基本一致,A、AB型直接与间接抗人-IgG均为阳性。原则上最好不输O型全血,从直接抗人-IgG实验分析,只要红细胞上存在不完全抗体,就一定会影响红细胞的寿命与质量。

人参白虎汤加减方对糖尿病大鼠血糖及免疫功能影响的实验研究

为观察人参白虎汤加减方对糖尿病大鼠的治疗效果 ,采用四氧嘧啶一次性腹腔注射法制造糖尿病大鼠模型 ,检测大鼠血糖、Ig G、Ig M含量 ,并与西药降糖灵作比较 ,结果表明 :1高剂量、低剂量均能降低糖尿病大鼠血糖 ,其降糖效果与降糖灵无明显差异。 2高剂量、低剂量均能不同程度地提高糖尿病大鼠 Ig G、Ig M含量 ,尤以高剂量疗效为佳。

水准网抗差估计试验及应用

通过分析和比较目前用于抗差估计中“最小值和法”和“IGG方案”理论在测量中应用的一般过程,讨论了粗差的检测和定位问题。亦即如何正确找出一组观测值中的粗差并消除粗差对平差结果的影响。通过大量的统计数据得出了概率意义更明确、计算更简便的改进计算方法,在指导实际生产中可以避免许多错误的产生。

Inflammatory Cytokine Secretion Status of Bone Marrow Cells and Clinical Significance in Immune-related Hematocytopenia

Objective To observe the expression of inlfammatory molecules in bone marrow immune cells of patients with immune-related hematocytopenia (IRH), and to investigate the immune mechanism and clinical signiifcance of the disease. Methods Total of 36 IRH patients were selected as observation group and 30 healthy people were taken as control group. Serum cytokines levels, activity of immunocytes and expression of HLA-DR were detected. Immune lfuorescence was applied to observe the expression state of immunologic molecules and cytokines in IRH patients. Results Serum cytokines were elevated in various degrees in observation group. Compared with the control group, the cytokines levels were significantly higher (P < 0.05). After treatement with immunosuppressive drugs, the serum levels of cytokines in observation group reduced to a level close to the control group. HLA-DR were upregulated in activated tissue basophils, eosinophils, dendritic cells (DC) and macrophages of bone marrow in IRH patients, and POX activity in these immunocytes of IRH was higher than that of the control group. Immune molecules were highly expressed in eosinophils, DC and macrophages. Conclusions It is demonstrated that antibodies or self-reactive lymphocytes were produced in IRH marrow, which would cause lesions of hemocytes, and lead to pathological process ifnally. Structure of hematopoietic cells mutated and these cells might be acted as target cells of immunocytes in the pathological process. Immunocytes could secrete inlfammatory factors and lead to immunologic injury of hemocyte.

Monthly gravity field recovery from GRACE orbits and K-band measurements using variational equations approach

The Gravity Recovery and Climate Experiment（GRACE） mission can significantly improve our knowledge of the temporal variability of the Earth＇s gravity field.We obtained monthly gravity field solutions based on variational equations approach from GPS-derived positions of GRACE satellites and K-band range-rate measurements.The impact of different fixed data weighting ratios in temporal gravity field recovery while combining the two types of data was investigated for the purpose of deriving the best combined solution.The monthly gravity field solution obtained through above procedures was named as the Institute of Geodesy and Geophysics（IGG） temporal gravity field models.IGG temporal gravity field models were compared with GRACE Release05（RL05） products in following aspects：（i） the trend of the mass anomaly in China and its nearby regions within 2005-2010; （ii） the root mean squares of the global mass anomaly during 2005-2010; （iii） time-series changes in the mean water storage in the region of the Amazon Basin and the Sahara Desert between 2005 and 2010.The results showed that IGG solutions were almost consistent with GRACE RL05 products in above aspects（i）-（iii）.Changes in the annual amplitude of mean water storage in the Amazon Basin were 14.7 ± 1.2 cm for IGG,17.1 ± 1.3 cm for the Centre for Space Research（CSR）,16.4 ± 0.9 cm for the GeoForschungsZentrum（GFZ） and 16.9 ± 1.2 cm for the Jet Propulsion Laboratory（JPL） in terms of equivalent water height（EWH）,respectively.The root mean squares of the mean mass anomaly in Sahara were 1.2 cm,0.9 cm,0.9 cm and 1.2 cm for temporal gravity field models of IGG,CSR,GFZ and JPL,respectively.Comparison suggested that IGG temporal gravity field solutions were at the same accuracy level with the latest temporal gravity field solutions published by CSR,GFZ and JPL.

O型血孕妇血清IgG抗A/B效价分布及茵枙黄颗粒治疗效果观察

目的:研究O型血孕妇血清IgG抗A/B效价分布、丈夫血型及孕妇年龄对抗体效价的影响以及茵枙黄颗粒的降效价作用。方法:选择初次就诊的O型血孕妇1 015例进行血清IgG抗A/B效价测定,分析丈夫血型及孕妇年龄与抗体效价的相关性,选取初检效价≥256者进行配对试验观察茵枙黄颗粒降效价的治疗效果。结果:1 015例初诊的O型血孕妇中,血清IgG抗A/B效价≥256者共453例(44.6%);丈夫血型为B型者抗体效价显著低于丈夫血型为AB型者(P<0.01);孕妇年龄和抗体效价之间呈微弱正相关(rs=0.09 381,P<0.01),25~30岁年龄段组效价水平显著低于31~36岁组和37岁以上组(P<0.01);与对照组相比较,茵枙黄颗粒治疗组末/初次效价比降低不明显(P>0.05)。结论:丈夫血型和孕妇年龄影响孕妇血清IgG抗A/B效价,茵枙黄颗粒单独使用时降效价作用不明显。

N-(β-Carboxypropionyl)luminol as a new chemiluminescence label in immunoassay

A new chemilurninescence label N-(β-carboxypropionyl)luminol (CPL) was used to label sheep anti-human IgG (SaHIgG).The labeled antibody was stable and could be detected at least down to 10-17~10-16 mol.The molar incorporation ratio was estimated to be 0.26 mol of CPL per mol of SaHIgG.There were no apparent changes in the immunoreactivity of the labeled SaHIgG and in the quantum efficiency of the CPL after labeling.