Objective:To study the effect of the direct anti-human globulin test on the clinical efficacy of blood transfusion patients.Methods:52 transfused patients were selected for this study,of which 26 cases with positive d...Objective:To study the effect of the direct anti-human globulin test on the clinical efficacy of blood transfusion patients.Methods:52 transfused patients were selected for this study,of which 26 cases with positive direct anti-human globulin tests were included in the positive group,and another 26 cases with negative direct anti-human globulin tests were included in the negative group.The apparent efficacy of the patients in the two groups after blood transfusion was compared.Results:After blood transfusion,the apparent efficacy of the negative group was significantly higher,P<0.05;in the positive group,the proportion of the predominantly multi-antibody group was the highest;after blood transfusion,the post-transfusion apparent efficacy of the simple IgG group was higher than that of the multi-antibody group,P<0.05;comparing the intensity of the different antibodies resulted in the 1+group,and the 3+to 4+groups were significantly lower after blood transfusion,P<0.05.Conclusion:The use of the direct antiglobulin test in transfused patients showed that patients with positive results would have better clinical efficacy.Direct anti-human globulin tests will have an impact on the clinical efficacy of blood transfusion in patients with positive results,so it is very important to carry out a direct anti-human globulin test on blood transfusion patients.展开更多
The correlation of serum arylesterase(PON1) activity on phenylacetate determined by an integrated method to clas-sical biochemical indexes of liver damage was investigated for the use of PON1 activity to evaluate live...The correlation of serum arylesterase(PON1) activity on phenylacetate determined by an integrated method to clas-sical biochemical indexes of liver damage was investigated for the use of PON1 activity to evaluate liver damage.PON1 reaction curve as absorbance at 270 nm for 0.20 mmol/L phenylacetate hydrolysis was analyzed by the integrated method to determine maximal PON1 reaction rate.Classical biochemical indexes of liver damage were determined routinely.The 95% confidence threshold of PON1 activity in sera from healthy individuals was 2.12 mkat/L [(4.73±1.31) mkat/L,n=105].PON1 activity in clinical sera was closely correlated to serum albumin,total protein and the ratio of albumin to globulins,but was weakly correlated to both direct and total bilirubin in serum.There were no correlations of PON1 activity to γ-glutamyltransferase,alkaline phos-phatase,alanine aminotransferase and aspartate aminotransferase.Among 127 clinical sera with PON1 activity>2.12 mkat/L,there were 92% healthy individuals examined by albumin,90% healthy individuals examined by total protein,88% healthy individuals examined by total bilirubin,86% healthy individuals examined by direct bilirubin and 64% healthy individuals examined by the ratio of albumin to globulins,respectively.In each group of healthy individuals judged by classical biochemical indexes of close correlation to PON1 activity,percentage of healthy individuals examined by PON1 activity was always >80%.These results suggested PON1 activity on phenylacetate estimated by the integrated method was also suitable for the evaluation of liver damage.展开更多
目的探究聚合酶链反应-序列特异性引物(PCR-SSP)技术联合抗人球蛋白卡式法在ABO和RhD血型鉴定困难患者中的应用。方法选取118例ABO与RhD血型鉴定困难患者血型标本,分别应用PCR-SSP技术、抗人球蛋白卡式法分析患者血型情况。结果118例血...目的探究聚合酶链反应-序列特异性引物(PCR-SSP)技术联合抗人球蛋白卡式法在ABO和RhD血型鉴定困难患者中的应用。方法选取118例ABO与RhD血型鉴定困难患者血型标本,分别应用PCR-SSP技术、抗人球蛋白卡式法分析患者血型情况。结果118例血型鉴定困难者采用抗人球蛋白卡式法联合PCR-SSP技术明确检出ABO血型113例,同时应用PCR-SSP可以确定ABO亚型,5例未检出血型的样品经PCR-SSP测序发现,基因分型分别为BO02、A205O01、A102B01、A201B01、B01B01,分别出现第7外显子640A>G杂合突变、第7外显子1009A>G杂合突变、第3外显子150G>C杂合突变、第6外显子240T>C、第7外显子431G>T杂合突变;采用抗人球蛋白卡式法联合PCR-SSP检出RhD血型114例,未检出的4例进行测序,1例显示为RhD weak D 779A/G突变;3例为RhD weak D type 4.0(包括602C/G、667T/G以及819G/A突变)。结论ABO和RhD血型鉴定困难患者应用PCR-SSP技术联合抗人球蛋白卡式法进行鉴定可以准确鉴定血型,并发现血型具体亚型,保证输血安全。展开更多
以小麦粉、酵母菌和植物乳酸菌M616(Lactobacillus plantarum M616)为原料制备酵母发酵面团、乳酸菌发酵面团和混合发酵面团,分别利用紫外分光光度法和凯氏定氮法测定面团发酵过程中清球蛋白含量。检测结果表明,两种方法测得发酵面团中...以小麦粉、酵母菌和植物乳酸菌M616(Lactobacillus plantarum M616)为原料制备酵母发酵面团、乳酸菌发酵面团和混合发酵面团,分别利用紫外分光光度法和凯氏定氮法测定面团发酵过程中清球蛋白含量。检测结果表明,两种方法测得发酵面团中的清球蛋白含量经多重比较均无显著差异(P>0.05)。乳酸菌发酵面团和混合发酵面团发酵过程中,清球蛋白含量随着发酵时间的延长显著下降,而酵母菌发酵面团发酵过程中,清球蛋白的降解较少。因此紫外分光光度法可用于发酵面团中清球蛋白含量的测定。展开更多
文摘Objective:To study the effect of the direct anti-human globulin test on the clinical efficacy of blood transfusion patients.Methods:52 transfused patients were selected for this study,of which 26 cases with positive direct anti-human globulin tests were included in the positive group,and another 26 cases with negative direct anti-human globulin tests were included in the negative group.The apparent efficacy of the patients in the two groups after blood transfusion was compared.Results:After blood transfusion,the apparent efficacy of the negative group was significantly higher,P<0.05;in the positive group,the proportion of the predominantly multi-antibody group was the highest;after blood transfusion,the post-transfusion apparent efficacy of the simple IgG group was higher than that of the multi-antibody group,P<0.05;comparing the intensity of the different antibodies resulted in the 1+group,and the 3+to 4+groups were significantly lower after blood transfusion,P<0.05.Conclusion:The use of the direct antiglobulin test in transfused patients showed that patients with positive results would have better clinical efficacy.Direct anti-human globulin tests will have an impact on the clinical efficacy of blood transfusion in patients with positive results,so it is very important to carry out a direct anti-human globulin test on blood transfusion patients.
基金Project (No. 30200266) supported by the National Natural Science Foundation of China
文摘The correlation of serum arylesterase(PON1) activity on phenylacetate determined by an integrated method to clas-sical biochemical indexes of liver damage was investigated for the use of PON1 activity to evaluate liver damage.PON1 reaction curve as absorbance at 270 nm for 0.20 mmol/L phenylacetate hydrolysis was analyzed by the integrated method to determine maximal PON1 reaction rate.Classical biochemical indexes of liver damage were determined routinely.The 95% confidence threshold of PON1 activity in sera from healthy individuals was 2.12 mkat/L [(4.73±1.31) mkat/L,n=105].PON1 activity in clinical sera was closely correlated to serum albumin,total protein and the ratio of albumin to globulins,but was weakly correlated to both direct and total bilirubin in serum.There were no correlations of PON1 activity to γ-glutamyltransferase,alkaline phos-phatase,alanine aminotransferase and aspartate aminotransferase.Among 127 clinical sera with PON1 activity>2.12 mkat/L,there were 92% healthy individuals examined by albumin,90% healthy individuals examined by total protein,88% healthy individuals examined by total bilirubin,86% healthy individuals examined by direct bilirubin and 64% healthy individuals examined by the ratio of albumin to globulins,respectively.In each group of healthy individuals judged by classical biochemical indexes of close correlation to PON1 activity,percentage of healthy individuals examined by PON1 activity was always >80%.These results suggested PON1 activity on phenylacetate estimated by the integrated method was also suitable for the evaluation of liver damage.
文摘目的探究聚合酶链反应-序列特异性引物(PCR-SSP)技术联合抗人球蛋白卡式法在ABO和RhD血型鉴定困难患者中的应用。方法选取118例ABO与RhD血型鉴定困难患者血型标本,分别应用PCR-SSP技术、抗人球蛋白卡式法分析患者血型情况。结果118例血型鉴定困难者采用抗人球蛋白卡式法联合PCR-SSP技术明确检出ABO血型113例,同时应用PCR-SSP可以确定ABO亚型,5例未检出血型的样品经PCR-SSP测序发现,基因分型分别为BO02、A205O01、A102B01、A201B01、B01B01,分别出现第7外显子640A>G杂合突变、第7外显子1009A>G杂合突变、第3外显子150G>C杂合突变、第6外显子240T>C、第7外显子431G>T杂合突变;采用抗人球蛋白卡式法联合PCR-SSP检出RhD血型114例,未检出的4例进行测序,1例显示为RhD weak D 779A/G突变;3例为RhD weak D type 4.0(包括602C/G、667T/G以及819G/A突变)。结论ABO和RhD血型鉴定困难患者应用PCR-SSP技术联合抗人球蛋白卡式法进行鉴定可以准确鉴定血型,并发现血型具体亚型,保证输血安全。
文摘以小麦粉、酵母菌和植物乳酸菌M616(Lactobacillus plantarum M616)为原料制备酵母发酵面团、乳酸菌发酵面团和混合发酵面团,分别利用紫外分光光度法和凯氏定氮法测定面团发酵过程中清球蛋白含量。检测结果表明,两种方法测得发酵面团中的清球蛋白含量经多重比较均无显著差异(P>0.05)。乳酸菌发酵面团和混合发酵面团发酵过程中,清球蛋白含量随着发酵时间的延长显著下降,而酵母菌发酵面团发酵过程中,清球蛋白的降解较少。因此紫外分光光度法可用于发酵面团中清球蛋白含量的测定。
