With environmental and food safety issues becoming one of the greatest concerns of the public, active plant compounds play more and more important roles in pest controlling. Fern are of great significance for applicat...With environmental and food safety issues becoming one of the greatest concerns of the public, active plant compounds play more and more important roles in pest controlling. Fern are of great significance for application as new pesticides due to their particular status in plant taxology and co-evolution with insects. In this study, methanol extracts were distilled from wild fern and gymnosperm of 54 species of 22 families collected from Shiwandashan Mountain in Guangxi Zhuang Autonomous Region of China. Insecticidal activities of the methanol extracts were assayed on both adult house fly (Musca domestica) and mosquito (Aedes albopictus). Significant insecticidal activities were exhibited in the methanol extracts of five species, Cupressus funebris (leaves and stems), Cycas acuminatissima (roots), Keteleeria fortunei (leaves and stems), Onychium japonicum (whole plant), and Pinus taiwanensis var. Daming shanensis (leaves and stems). Applications of the extracts of these plants resulted in higher than 50% mortalities in 4th instar larvae of A. albopictus at 24 h after treatment. The methanol extracts from 13 species possessed insecticidal activities against the adult of M. domestica at 48 h after treatment with higher than 90% mortalities. The extensive screening results showed that these fern and gymnosperm were highly potential to be botanical insecticides. The findings provide a feasible and valuable basis for further investigation.展开更多
Plants with bioactive properties are greatly useful in preventing and controlling blood-sucking and disease-vector invertebrates, particularly in developing countries and low-income communities. Their application is a...Plants with bioactive properties are greatly useful in preventing and controlling blood-sucking and disease-vector invertebrates, particularly in developing countries and low-income communities. Their application is a promising alternative to synthetic compounds whose use remains a health, environmental, and economic challenge. However, many are still unknown and unvalued, while others are becoming ignored and threatened. The main objective of this ethnobotanical study is to identify and characterize indigenous and locally grown plants against blood-sucking and disease-vector insects. Salient opportunities and challenges of using these plants are documented and discussed. Semi-structured interviews, using a prepared questionnaire, were conducted with 228 informants. The consensus index (CI) was calculated to analyze the reliability of the collected information. The identified 31 anti-insect plant species belong to 20 botanical families, four morphological categories, and six habitat types. They can be categorized as insecticidal plants (42% of the total), insect repellent (42% of the total), and both insecticidal and insect repellent (16% of the total). More than 54% of these are still abundant in the study area, while about 35.5% have become rare and difficultly accessible. Based on the numerical importance of related anti-insect plant species, the seven targeted blood-sucking insects range in the following decreasing order: Jiggers (16 species) > Fire Ants (9 species) > Flies (8 plants) > Mosquitoes (4 species) > Fleas (2 species) > Bedbugs (1 species) > lice (0 species). The three most commonly used plants, with the highest confirmation indices, are Tetradenia riparia (ICs = 0.712), Eucalyptus globulus subsp. maidenii (ICs = 0.302), and Solanum aculeastrum (ICs = 0.288). The antimicrobial role of many locally grown anti-insect plants and the multiple other associated valorization possibilities are ignored by most informants. Domesticating, propagating, protecting, and promoting the sustainable use of these plants would be an appropriate route for their conservation and continued availability.展开更多
An excitatory anti-insect toxin from BmK scorpion, named BmK 16, has been purified and crystallized recently. Two crystal forms have been obtained by using ammonium sulfate as precipitant at different pH, one of which...An excitatory anti-insect toxin from BmK scorpion, named BmK 16, has been purified and crystallized recently. Two crystal forms have been obtained by using ammonium sulfate as precipitant at different pH, one of which is single crystal. Diffraction data have been collected for the single crystal on a Mar Research 345 Image Plate. The analyses of the data show that the crystal can diffract to 0.28 nm resolution and belongs to the space group C2 with unit cell parameters a = 4.65 nm, b = 8.52 nm, c= 3.26 nm, α= γ= 90oooooooo, β= 110.5°. There are 2 molecules per asymmetric unit according to its unit cell volume and molecular weight.展开更多
用石油醚、乙醚、乙酸乙酯3种溶剂对菊苣叶片进行平行提取,以粘虫（Mythimna separate Walker）为供试昆虫,应用饲喂称重法测定菊苣叶片不同溶剂提取物对其生长发育及拒食的影响。结果表明,3种机溶剂提取物处理的幼虫体重均显著低于对照...用石油醚、乙醚、乙酸乙酯3种溶剂对菊苣叶片进行平行提取,以粘虫（Mythimna separate Walker）为供试昆虫,应用饲喂称重法测定菊苣叶片不同溶剂提取物对其生长发育及拒食的影响。结果表明,3种机溶剂提取物处理的幼虫体重均显著低于对照组;从第3~5天,3种有机溶剂提取物处理的幼虫体重之间差异显著,其中乙酸乙酯提取物校正死亡率最高,达52.05%;3种有机溶剂处理的幼虫期比对照延长了4~10 d,且蛹重略高于对照组;3种有机溶剂对粘虫拒食均有显著效果。展开更多
基金funded by the National Nature Science Foundation of China (30571235)the Industry Schemes of the Ministry of Agriculture,China(200903052)
文摘With environmental and food safety issues becoming one of the greatest concerns of the public, active plant compounds play more and more important roles in pest controlling. Fern are of great significance for application as new pesticides due to their particular status in plant taxology and co-evolution with insects. In this study, methanol extracts were distilled from wild fern and gymnosperm of 54 species of 22 families collected from Shiwandashan Mountain in Guangxi Zhuang Autonomous Region of China. Insecticidal activities of the methanol extracts were assayed on both adult house fly (Musca domestica) and mosquito (Aedes albopictus). Significant insecticidal activities were exhibited in the methanol extracts of five species, Cupressus funebris (leaves and stems), Cycas acuminatissima (roots), Keteleeria fortunei (leaves and stems), Onychium japonicum (whole plant), and Pinus taiwanensis var. Daming shanensis (leaves and stems). Applications of the extracts of these plants resulted in higher than 50% mortalities in 4th instar larvae of A. albopictus at 24 h after treatment. The methanol extracts from 13 species possessed insecticidal activities against the adult of M. domestica at 48 h after treatment with higher than 90% mortalities. The extensive screening results showed that these fern and gymnosperm were highly potential to be botanical insecticides. The findings provide a feasible and valuable basis for further investigation.
文摘Plants with bioactive properties are greatly useful in preventing and controlling blood-sucking and disease-vector invertebrates, particularly in developing countries and low-income communities. Their application is a promising alternative to synthetic compounds whose use remains a health, environmental, and economic challenge. However, many are still unknown and unvalued, while others are becoming ignored and threatened. The main objective of this ethnobotanical study is to identify and characterize indigenous and locally grown plants against blood-sucking and disease-vector insects. Salient opportunities and challenges of using these plants are documented and discussed. Semi-structured interviews, using a prepared questionnaire, were conducted with 228 informants. The consensus index (CI) was calculated to analyze the reliability of the collected information. The identified 31 anti-insect plant species belong to 20 botanical families, four morphological categories, and six habitat types. They can be categorized as insecticidal plants (42% of the total), insect repellent (42% of the total), and both insecticidal and insect repellent (16% of the total). More than 54% of these are still abundant in the study area, while about 35.5% have become rare and difficultly accessible. Based on the numerical importance of related anti-insect plant species, the seven targeted blood-sucking insects range in the following decreasing order: Jiggers (16 species) > Fire Ants (9 species) > Flies (8 plants) > Mosquitoes (4 species) > Fleas (2 species) > Bedbugs (1 species) > lice (0 species). The three most commonly used plants, with the highest confirmation indices, are Tetradenia riparia (ICs = 0.712), Eucalyptus globulus subsp. maidenii (ICs = 0.302), and Solanum aculeastrum (ICs = 0.288). The antimicrobial role of many locally grown anti-insect plants and the multiple other associated valorization possibilities are ignored by most informants. Domesticating, propagating, protecting, and promoting the sustainable use of these plants would be an appropriate route for their conservation and continued availability.
文摘An excitatory anti-insect toxin from BmK scorpion, named BmK 16, has been purified and crystallized recently. Two crystal forms have been obtained by using ammonium sulfate as precipitant at different pH, one of which is single crystal. Diffraction data have been collected for the single crystal on a Mar Research 345 Image Plate. The analyses of the data show that the crystal can diffract to 0.28 nm resolution and belongs to the space group C2 with unit cell parameters a = 4.65 nm, b = 8.52 nm, c= 3.26 nm, α= γ= 90oooooooo, β= 110.5°. There are 2 molecules per asymmetric unit according to its unit cell volume and molecular weight.
文摘用石油醚、乙醚、乙酸乙酯3种溶剂对菊苣叶片进行平行提取,以粘虫（Mythimna separate Walker）为供试昆虫,应用饲喂称重法测定菊苣叶片不同溶剂提取物对其生长发育及拒食的影响。结果表明,3种机溶剂提取物处理的幼虫体重均显著低于对照组;从第3~5天,3种有机溶剂提取物处理的幼虫体重之间差异显著,其中乙酸乙酯提取物校正死亡率最高,达52.05%;3种有机溶剂处理的幼虫期比对照延长了4~10 d,且蛹重略高于对照组;3种有机溶剂对粘虫拒食均有显著效果。
