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Purification and Characterization of Flammulin,a Basic Protein with Anti-tumor Activities from Flammulina velutipes 被引量:10
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作者 陈畅 薛久刚 +3 位作者 周凯松 李彦 张晗星 张长铠 《Journal of Chinese Pharmaceutical Sciences》 CAS 2003年第2期60-65,共6页
Aim To purify and characterize flammulin, a basic protein with anti-tumoractivities. Methods Ammonium sulfate, ethanol fractionation and column chromatography were used forseparation and purification. Electrophoretic ... Aim To purify and characterize flammulin, a basic protein with anti-tumoractivities. Methods Ammonium sulfate, ethanol fractionation and column chromatography were used forseparation and purification. Electrophoretic analysis, amino acid analysis, and MS of flammulin werecarried out. Results Flammulin was purified to electrophoretic homogeneity and crystallized. With amolecular mass of 19891.13 Da, pI 8.9, λ_(max) = 276 - 278 nm, λ_(min) = 250 nm, flammulin wascharacterized by its lack of methionine. Fingerprint mapping of flammulin was determined by MALDI-MSfollowing in-gel protease digestion; no close matches were identified. Conclusion Flammulin waspurified to electrophoretic homogeneity, and its characteristics are discussed for the first time. 展开更多
关键词 anti-tumor activities flammulin flammulina velutipes PURIFICATION CHARACTERIZATION mass spectrometry
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Anti-tumor activities of macromolecular fractions of fresh gecko vivo and their induction of Bel-7402 cell differentiation 被引量:6
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作者 Yuxia Wang Xiangxiang Gu +3 位作者 Hongmei Deng Di Geng Huaying Sun Chunmei Wang 《Journal of Traditional Chinese Medical Sciences》 2017年第4期328-335,共8页
Objective:To investigate the anti-tumor effect of macromolecular fractions of fresh gecko (M-AG) in vivo and their differentiation-inducing activity in Bel-7402 cells in vitro.Methods:An H22 hepatocarcinoma-bearing mo... Objective:To investigate the anti-tumor effect of macromolecular fractions of fresh gecko (M-AG) in vivo and their differentiation-inducing activity in Bel-7402 cells in vitro.Methods:An H22 hepatocarcinoma-bearing mouse model was used to evaluate the anti-tumor activity of M-AG samples.The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay was applied to analyze cell viability.Cell morphology was observed by phase contrast microscopy.The quantity of the alpha-fetoprotein was detected by a radioimmunoassay.Chromatometry was used to assay the albumin quantity.Activities of alkaline phosphatase and γ-glutamyl trans-peptidase were measured by biochemical methods.Finally,western blotting was applied to assess proteins in the mitogen-activated protein kinase (MAPK) signaling pathway.Results:Macromolecular fractions of fresh gecko exerted a significant anti-tumor effect in mice.The inhibition rate of tumor growth was 63% in the moderate M-AG dose group.Cells treated with M-AG displayed a differentiated state.The treatment lowered alphafetoprotein secretion and significantly decreased the activities of γ-glutamyl trans-peptidase and alkaline phosphatase in Bel-7402 cells.In contrast,M-AG increased the amount of albumin in the cell culture medium.All biochemical indices demonstrated that M-AG induced Bel7402 cell differentiation.Western blotting showed no changes in the quantities of extracellular signal-regulated kinase (ERK) 1/2,p38MAPK,or c-Jun N-terminal protein kinase 1/2.However,M-AG significantly activated the phosphorylation of ERK1/2 in a dose-dependent manner.In addition,M-AG had no significant influence on the expression of nuclear factor-kappa B.Conclusion:Macromolecular fractions of fresh gecko has an anti-tumor activity in H22 hepatocarcinoma-bearing mice in vivo and inhibits Bel-7402 cell proliferation in vitro by inducing cell differentiation related to activation of ERK1/2. 展开更多
关键词 Macromolecular FRACTIONS of FRESH GECKO H22 hepatocarcinoma-bearing mice anti-tumor activity Induced differentiation ERK1/2
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A heteropolysaccharide from Rhodiola rosea L.:preparation,purification and anti-tumor activities in H22-bearing mice 被引量:1
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作者 Yaru Wu Qing Wang +3 位作者 Huiping Liu Lulu Niu Mengyu Li Qi Jia 《Food Science and Human Wellness》 SCIE CSCD 2023年第2期536-545,共10页
Numerous polysaccharides isolated from plants have been used to augment traditional drugs in the treatment of cancer.In order to explore the influence to hepatocellular carcinoma,a novel cold water-soluble polysacchar... Numerous polysaccharides isolated from plants have been used to augment traditional drugs in the treatment of cancer.In order to explore the influence to hepatocellular carcinoma,a novel cold water-soluble polysaccharide was separated from Rhodiola rosea L.root(RLP)and then its structure and anti-cancer activities were tested.The chemical compositions and high performance gel permeation chromatography(HPGPC)results indicated that RLP was an acid heteropolysaccharide with the molecular weight of about1.15×10~6 Da.Furthermore,ion chromatography(IC),Fourier transform infrared(FT-IR)and nuclear magnetic resoance(NMR)further indicated that RLP was main composed of→2,4)-α-Rha(1→,→5)-α-L-Araf-(1→,α-D-Glu,→6)-β-D-Galp-(1→,β-D-Man and→4)-α-GalpA-(1→.In vivo antitumor activities of RLP were carried out by using H22 tumor-bearing mice model.The results shown that RLP(100 and 300 mg/kg)could inhibit tumor growth of H22 cells from 23.59%to 45.52%and protect thymuses and spleen without damage.In addition,according to cell cycle,AV-FITC/PI and JC-1,RLP could induce dose-dependent apopto sis of H22 cells via S phase arrested which was through a mitochondrial related pathway.Our data indicated that RLP has a broader application prospect in anti-tumor preparations. 展开更多
关键词 POLYSACCHARIDE Antitumor activity Rhodiola rosea L. Structure analysis
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Synthesis and Anti-Tumor Activities of Fluoride-Containing Gossypol Derivatives Compounds
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作者 Liying Zeng Yijian Deng +3 位作者 Lidong Weng Zhijun Yang Huoji Chen Qiang Liu 《Natural Science》 2017年第9期312-318,共7页
We report herein the design and synthesis of a series of novel fluoride-containing gossypol derivatives by the condensation reaction of gossypol with fluoride-containing aromatic amine. These fluoride-containing gossy... We report herein the design and synthesis of a series of novel fluoride-containing gossypol derivatives by the condensation reaction of gossypol with fluoride-containing aromatic amine. These fluoride-containing gossypol derivatives were characterized by IR, 1H-NMR and high resolution mass spectral data, then screened as antitumor agents against three human cancer cell lines (HeLa, A-549 and BGC-823) and a normal cell line (VEC) in vitro by using MTT cell proliferation assays. Results revealed that compounds 3a, 3c and 3f exhibited superior anticancer activity against HeLa, compounds 3b,3c, 3e and 3f exhibited superior anticancer activity against A-549, compounds 3b, 3c and 3f exhibited superior anticancer activity against BGC-823 compared to gossypol. In particular, fluorine substituent at the para positions of the phenyl ring showed remarkable inhibitory effects on HeLa?(3c: IC50 = 14.2 μM, 3f:?IC50 = 8.34 μM), A-549(3c: IC50 = 6.32 μM, 3f: IC50 = 9.76 μM) and BGC-823 cells?(3c: IC50 = 8.62 μM, 3f: IC50 = 4.36 μM). Furthermore, all the compounds 3a-3f exhibited lessened cytotoxicity against VEC compared to gossypol. 展开更多
关键词 GOSSYPOL DERIVATIVES Fluoride-Containing SYNTHESIS anti-tumor activity
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Anti-tumor Activities of Novel Estrogen Compound 17aα-D-Homo-Ethynylestradiol-3-Acetate
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作者 周则卫 唐卫生 +3 位作者 沈秀 韩英 王晓雪 张良安 《Chinese Journal of Cancer Research》 SCIE CAS CSCD 2008年第1期17-20,共4页
Objective: To study the anti-tumor activities of novel estrogen compound 17a α-D-homo-ethynylestradiol-3-acetate in vitro and in vivo. Methods: In vitro anti-tumor activity was assayed in adenoma cells A549 and hum... Objective: To study the anti-tumor activities of novel estrogen compound 17a α-D-homo-ethynylestradiol-3-acetate in vitro and in vivo. Methods: In vitro anti-tumor activity was assayed in adenoma cells A549 and human liver cancer cells Bel-7402 using MTT method, and half-inhibitory concentration (IC50) were observed. In vivo the pulmonary adenoma LA795 cells was selected and the conventional assay method of anti-tumor activity was employed. 5, 7.5, 10 mg/kg of 17a α-D-homo-ethynylestradiol-3-acetate was administered by i.p., and tumor-inhibitory rate, thymus and spleen indexes, bone marrow cells (BMC) were observed. Results: IC50 of 17a α-D-homo-ethynylestradiol-3-acetate in vitro for A549 and Bel-7402 cells were 12.28 μg/ml and 17.79 μg/ml, respectively. In vivo the highest tumor-inhibitory rates for LA795 was 60.0% (P〈0.01). The drug had hardly any side-effect in spleen indexes, thymus indexes, and BMC compared with control mice. Nevertheless, compared with the positive control drug cyclophosphamide (CY), thymus and spleen indexes, BMC showed obvious differences (P〈0.01). Conclusion: 17a α-D-homo-ethynylestradiol-3-acetate has obvious anti-tumor activities in vitro and in vivo with low side-effect, thus worth further investigation. 展开更多
关键词 17a α-D-homo-ethynylestradioi-3-acetate ESTROGEN anti-tumor PHARMACOLOGY
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Cranberry cultivated in China:UPLC-Q-TOF-MS analysis of its acidic ethanol extract and assessment of its anti-bacterial and anti-tumor activities in vitro
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作者 Changhua Li Lin Wang +6 位作者 Dianwen Wei Gaosheng Hu Xiaoning Zhang Zhihong Lou Qiong Zhang Jingming Jia Yongsheng Hou 《Journal of Polyphenols》 2019年第1期50-61,共12页
Cranberry,the fruit of Vaccinium macrocarpon Aiton,is becoming popular all over the world,due to its pleasant taste,nutraceutical value and biological activities.For a long time,98%of its yield has been from America.S... Cranberry,the fruit of Vaccinium macrocarpon Aiton,is becoming popular all over the world,due to its pleasant taste,nutraceutical value and biological activities.For a long time,98%of its yield has been from America.Since being introduced to China as an import in recent decades,cranberry has become a rapid success in the food industry even with the high transportation cost.Recently,V.macrocarpon was cultivated in the Northeast of China,and the area of cultivation has increased dramatically.Therefore,it is important to systematically determine the useful compounds and their activity in this“Made-in-China”cranberry.In the present study,the content of total phenolics,anthocyanins,proanthocyanidins,sugars and organic acids was determined.Our results demonstrated that domestic cranberry is a rich source of phenolics,anthocyanins,proanthocyanidins,sugars and organic acids.Analysis of the chemical composition of an acidic ethanol extract by UPLC-QTOF-MS identified 21 compounds as well as the contents determination of catechin and cyanidin-3-O-glucoside using RPHPLC.Furthermore,cranberry extract was tested for its anti-bacterial activity against five clinically important pathogens,as well as its inhibitory effect on the proliferation and migration of cancer cells in vitro.Our results demonstrated that cranberry produced in Chinese cultivation areas is a rich source of bioactive compounds and exhibited in vitro anti-microbial and anti-tumor activities.Our work provides essential information about the quality of domestic cranberry and facilitates the development of related industries. 展开更多
关键词 Vaccinium macrocarpon UPLC-Q-TOF-MS content determination proanthocyanidin total phenolics Chinese cranberry anti-cancer activity anti-bacterial activity
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CuBr-promoted domino Biginelli reaction for the diastereoselective synthesis of bridged polyheterocycles:Mechanism studies and in vitro anti-tumor activities
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作者 Mengting Zeng Ying Xue +3 位作者 Yunan Qin Fen Peng Quan Li Ming-Hua Zeng 《Chinese Chemical Letters》 SCIE CAS CSCD 2022年第11期4891-4895,共5页
The low-cost CuBr-promoted domino Biginelli reaction among readily available ketones,salicylaldehyde derivatives and 3-amino-1,2,4-triazole was studied under solvothermal conditions,giving the novel bridged polyhetero... The low-cost CuBr-promoted domino Biginelli reaction among readily available ketones,salicylaldehyde derivatives and 3-amino-1,2,4-triazole was studied under solvothermal conditions,giving the novel bridged polyheterocycles bearing two or three stereocenters depending on the starting ketones.This multicomponent reaction proceeded with high diastereoselectivity(dr>20:1)based on a combined~1 H NMR,crystallographic and supercritical fluid chromatographic(SFC)analysis of the product.Time-dependent high-resolution mass spectrometry(HRMS)was performed to track the reaction process,and several key intermediates were identified,leading to the drawing of a plausible reaction mechanism.Density functional theory(DFT)calculation was supplemented,and two reaction pathways were differentiated.Moreover,in vitro antitumor activity was evaluated using HeLa and HepG2 cell lines,and two of these polyheterocycles demonstrated promising activities against HepG2 cells with EC50 down to 10μmol/L.Additionally,ESI-MS/MS studies on all the polyheterocycles suggest a common fragmentation pathway(loss of one molecule of amino-triazole)they shared,providing the first-hand fragmentation rules for future rapid structural identification of them.The multicomponent domino reaction presented here may offer prospects for future design of more efficient strategies to access medicinally important bridged polyheterocycles. 展开更多
关键词 Multicomponent reaction Domino reaction Biginelli reaction Bridged polyheterocycles Reaction mechanism anti-tumor activities
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2024 Adult Compendium of Physical Activities:A third update of the energy costs of human activities 被引量:10
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作者 Stephen D.Herrmann Erik A.Willis +10 位作者 Barbara E.Ainsworth Tiago V.Barreira Mary Hastert Chelsea L.Kracht John M.Schuna Jr. Zhenghui Cai Minghui Quan Catrine Tudor-Locke Melicia C.Whitt-Glover David R. Jacobs Jr. 《Journal of Sport and Health Science》 SCIE CSCD 2024年第1期6-12,共7页
Background:The Compendium of Physical Activities was published in 1993 to improve the comparability of energy expenditure values assigned to self-reported physical activity(PA)across studies.The original version was u... Background:The Compendium of Physical Activities was published in 1993 to improve the comparability of energy expenditure values assigned to self-reported physical activity(PA)across studies.The original version was updated in 2000,and again in 2011,and has been widely used to support PA research,practice,and public health guidelines.Methods:This 2024 update was tailored for adults 19-59 years of age by removing data from those≥60 years.Using a systematic review and supplementary searches,we identified new activities and their associated measured metabolic equivalent(MET)values(using indirect calorimetry)published since 2011.We replaced estimated METs with measured values when possible.Results:We screened 32,173 abstracts and 1507 full-text papers and extracted 2356 PA energy expenditure values from 701 papers.We added303 new PAs and adjusted 176 existing MET values and descriptions to reflect the addition of new data and removal of METs for older adults.We added a Major Heading(Video Games).The 2024 Adult Compendium includes 1114 PAs(912 with measured and 202 with estimated values)across 22 Major Headings.Conclusion:This comprehensive update and refinement led to the creation of The 2024 Adult Compendium,which has utility across research,public health,education,and healthcare domains,as well as in the development of consumer health technologies.The new website with the complete lists of PAs and supporting resources is available at https://pacompendium.com. 展开更多
关键词 ADULTS Energy expenditure EXERCISE MET Physical activities
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A brief history of the Compendium of Physical Activities 被引量:2
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作者 Barbara E.Ainsworth Stephen D.Herrmann +2 位作者 David R.Jacobs Jr. Melicia C.Whitt-Glover Catrine Tudor-Locke 《Journal of Sport and Health Science》 SCIE CSCD 2024年第1期3-5,共3页
The Compendium of Physical Activities(Compendium)was developed to address consistent assignment of physical activity(PA)intensity values used in PA epidemiology research of the association between PA and health outcom... The Compendium of Physical Activities(Compendium)was developed to address consistent assignment of physical activity(PA)intensity values used in PA epidemiology research of the association between PA and health outcomes.1The known protective effects of PA on incident health outcomes traces to the mid-1900s,with over 50 studies examining coronary heart disease(CHD)as the outcome of interest. 展开更多
关键词 EPIDEMIOLOGY activities protective
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Synthesis and anti-tumor activities of 5-carbohydrate modified cyclophosphamide derivates 被引量:1
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作者 郑愉 孟祥豹 +3 位作者 李树春 黄河清 李中军 李庆 《Journal of Chinese Pharmaceutical Sciences》 CAS 2010年第5期327-340,共14页
Novel prodrugs of cyclophosphamide 1a and 1b, which comprised the galactosyl moiety, the key fraction of cyclophosphamide derivates, and the linker 4-hydroxy benzaldehyde, were synthesized. These compounds were antici... Novel prodrugs of cyclophosphamide 1a and 1b, which comprised the galactosyl moiety, the key fraction of cyclophosphamide derivates, and the linker 4-hydroxy benzaldehyde, were synthesized. These compounds were anticipated to exhibit amplified anti-tumor activity and targeting ability. 展开更多
关键词 CYCLOPHOSPHAMIDE GALACTOSIDE Hepatocyte targeting anti-tumor
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Discovery of Highly Oxidized Abietane Diterpenoids from the Roots of Euphorbia fischeriana with Anti-tumor Activities 被引量:3
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作者 Jiang-Chun Wei Yu-Ning Gao +7 位作者 Dong-Dong Wang Xiao-Yu Zhang San-Peng Fan Te-Ri-Gen Bao Xiao-Xu Gao Gao-Sheng Hu An-Hua Wang Jing-Ming Jia 《Chinese Journal of Chemistry》 SCIE CAS CSCD 2021年第11期2973-2982,共10页
Four previously undescribed ent-abietane diterpenoids(1—4),along with eleven known analogues(5—15),were isolated from the roots of wild Euphorbia fischeriana.Their gross structures were determined via extensive spec... Four previously undescribed ent-abietane diterpenoids(1—4),along with eleven known analogues(5—15),were isolated from the roots of wild Euphorbia fischeriana.Their gross structures were determined via extensive spectroscopic data,and the absolute configurations were elucidated by means of single-crystal X-ray diffraction analysis,Rh2(OCOCF3)4-induced CD spectrum and ECD calculations.Their cervical carcinoma inhibition activities counteract HeLa cells were screened by MTT assay,and the structure-activity relationships were further examined.The results demonstrated that 2(IC50 value at 3.75μmol/L)was more potent than cisplatin.The underlying mechanism study revealed that 2 could distinctly induce apoptosis accompanied by increasing reactive oxygen species(ROS)production,Ca^(2+)influx and decreasing mitochondrial membrane potential.Furthermore,signal pathways including MAPKs/AKT might play a significant role in 2-induced cervical cancer cells death. 展开更多
关键词 Euphorbia fischeriana DITERPENOIDS Structure elucidation Biological activity anti-tumor
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Preparation of kakkatin derivatives and their anti-tumor activity
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作者 Yu-Ying Jiang Hui-Hua Dong +3 位作者 Wen-Ting Zhou Jia-Zi Luo Xian Wei Yan-Qiang Huang 《World Journal of Clinical Oncology》 2024年第8期1078-1091,共14页
BACKGROUND Modern pharmacological studies have confirmed that plant-derived compounds from Puerariae flos(PF)has significant biological activities against liver damage,tumors and inflammation.Kakkatin is an isoflavone... BACKGROUND Modern pharmacological studies have confirmed that plant-derived compounds from Puerariae flos(PF)has significant biological activities against liver damage,tumors and inflammation.Kakkatin is an isoflavone polyphenolic compound isolated from PF flower.However,the effect of kakkatin and its derivatives on anti-tumor has not been well explored.AIM To design and synthesize a kakkatin derivative[6-(hept-6-yn-1-yloxy)-3-(4-hydroxyphenyl)-7-methoxy-4H-chromen-4-one(HK)]to explore its anti-tumor biological activity.METHODS Hept-6-yn-1-yl ethanesulfonate was introduced to replace hydrogen at the hydroxyl position of kakkatin phenol,and the derivative of kakkatin was prepared;the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide was used to detect cell viability,a clone formation assay was adopted to detect cell proliferation,apoptosis,necrosis,and cell cycles were analyzed by Annexin V/propidium iodide staining and flow cytometry.Cell migration and invasion ability were evaluated by cell scratch assay and transwell assay.The potential mechanism of HK on hepatocellular carcinoma(HCC)SMMC-7721 cells was explored through network pharmacology and molecular docking,and finally real-time PCR assays was used to verify the potential targets and evaluate the biological activity of HK.RESULTS Compared with kakkatin,the modified HK did not significantly increase the inhibitory activity of gastric cancer MGC803 cells,but the inhibitory activity of HCC SMMC-7721 cells was increased by about 30 times,with an IC50 value of 2.5μM,and the tumor inhibition effect was better than cisplatin,which could significantly inhibit the cloning,invasion and metastasis of HCC SMMC-7721 cells,and induce apoptosis and G2/M cycle arrest.Its mechanism of action is mainly related to the upregulation of PDE3B and NFKB1 target proteins in the cAMP pathway.CONCLUSION HK have a significant inhibitory effect on HCC SMMC-7721 cells,and the targets of their action may be PDE3B and NFKB1 proteins in the cAMP pathway,making it a good lead drug for the treatment of HCC. 展开更多
关键词 Kakkatin DERIVATIVE Hepatocellular carcinoma anti-tumor Biological activity
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Screening for Anti-tumor Activity of Fractions from Buddleja officinalis Maxim
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作者 Tong LI Xin XIE +9 位作者 Liyuan LI Aipeng LI Zhidong LEI Tingting HE Sirui MO Shangfeng HUANG Yao LIN Suoyi HUANG Lixiang LU Shiyou ZHOU 《Medicinal Plant》 2024年第2期48-50,56,共4页
[Objectives]The anti-tumor activity of fractions from Buddleja officinalis Maxim.by petroleum ether,ethyl acetate,n-butanol and water solvent was studied.[Methods]The ethanol extract from B.officinalis Maxim.was extra... [Objectives]The anti-tumor activity of fractions from Buddleja officinalis Maxim.by petroleum ether,ethyl acetate,n-butanol and water solvent was studied.[Methods]The ethanol extract from B.officinalis Maxim.was extracted and then concentrated with petroleum ether,ethyl acetate,n-butanol and water,respectively,and the extracts were obtained.The inhibitory effects of the four different fractions on the growth of three tumor cell lines in vitro were detected by CCK-8 method,and the median inhibitory concentration(IC 50 value)was calculated.[Results]The four fractions inhibited the growth of the three tumor cell lines in vitro,among which the n-butanol fraction had the best anti-tumor activity.The IC 50 values of the n-butanol fraction on human gastric cancer(SGC-7901),human breast cancer(MCF-7)and human liver cancer(BEL-7404)cell lines were 0.08,1.58 and 0.12 mg/mL,respectively.[Conclusions]Petroleum ether,ethyl acetate,n-butanol and water fractions from the ethanol extract of B.officinalis Maxim.had certain anti-tumor effects,and the n-butanol fraction had the best anti-tumor activity. 展开更多
关键词 Buddleja officinalis Maxim. Tumor cells anti-tumor activity screening
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Antimicrobial and Antioxidant Activities of a Brown Wood Rotting Mushroom Piptoporellus baudonii from Benin (Tropical Africa)
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作者 Olyvia Gwladys Fadeyi Boris Armel Olou +6 位作者 Roland Tchuenteu Tchuenguem Bienvenu Tsakem Pierre Valery Kemdoum Sinda Rémy Bertrand Teponno Léon Azefack Tapondjou Jean Paul Dzoyem Nourou Soulemane Yorou 《Advances in Biological Chemistry》 CAS 2024年第5期145-170,共26页
Piptoporellus baudonii, previously known as Laetiporus baudonii, is an African species that was considered to be a sister species to Laetiporus sulphureus, another European species known for its medicinal value. While... Piptoporellus baudonii, previously known as Laetiporus baudonii, is an African species that was considered to be a sister species to Laetiporus sulphureus, another European species known for its medicinal value. While much is known about the edibility and antimicrobial properties of L. sulphureus, African species like P. baudonii remain understudied. This study investigated the antimicrobial and antioxidant properties of P. baudonii extracts (powder maceration) prepared using ethanol, methanol and water with fractions obtained via differential solubility in hexane, ethyl acetate and n-butanol. Before the antimicrobial analysis, the study material was accurately identified using both morphology and molecular techniques. Antimicrobial activity was tested against fungi, gram-positive, and gram-negative bacteria using a broth serial microdilution method, while antioxidant activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and Ferric Reducing Antioxidant Power FRAP methods. Phylogenetic analysis confirmed the specimen as P. baudonii, with genetic material from Benin grouping it with other P. baudonii from Tanzania and other unknown regions, forming a well-supported clade (100/100). The ethanol (1.71), methanol (2.41) extracts, along with ethyl acetate (1.36), n-butanol (1.18), and hexane (12.91) fractions showed significant antioxidant activity with EC50 values below 20 µg∙mL−1. The highest antimicrobial inhibition was seen in the n-butanol (58%) and ethyl acetate (54%) fractions, followed by ethanol (49%) and hexane (48%). Methanol exhibited the lowest inhibition (46.10%). These values were compared to the standard (Vitamin C). The examined extracts demonstrated high bactericidal properties, with an MBC/MIC ratio (R) of 1 to 4, particularly effective ethyl acetate against Escherichia coli (R = 2) and ethanol extract with strong activity against Enterococcus faecalis (R = 4). Further chemical and cytotoxicity studies are warranted to fully explore the pharmaceutical potential of P. baudonii. 展开更多
关键词 FUNGI Piptoporellus baudonii Antimicrobial activity Antioxidant activity DNA Extraction BENIN
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Hydrolytic and Antimicrobial Activities of Bacteria Isolated from Fermented Peppers Sold in Markets at Brazzaville, Republic of the Congo
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作者 Faly Armel Soloka Mabika Elgie Viennechie Gatsé +3 位作者 Ngo Itsouhou Tarcisse Baloki Ngoulou Mérite Berthelie Mouanda Nzoussi Nguimbi Etienne 《Journal of Biosciences and Medicines》 2024年第9期215-234,共20页
Microorganisms are omnipresent in all environments and play mainly the role of transformers, thanks to the multiple enzymes they are able to produce. In order to valorize fermented foods in the Republic of the Congo, ... Microorganisms are omnipresent in all environments and play mainly the role of transformers, thanks to the multiple enzymes they are able to produce. In order to valorize fermented foods in the Republic of the Congo, this work aimed to characterize and study some properties of microorganisms isolated from samples of peppers sold in three markets of Brazzaville. A numeration of the total aerobic mesophilic flora (TAMF) was made in a solid medium, allowing the evaluation of each sample’s microbial concentration. The microbial mass varied from 2.8 × 105 CFU/g for the Ouénzé sample to 1.8 × 104 CFU/g for the Total sample and 2 × 104 CFU/g for the Moungali market sample. The evaluation of the enzymatic properties of the Bacillus isolates showed that 68.42% were capable of producing cellulases and 78.94% were capable of producing amylases and proteases. Antimicrobial activities revealed that 63.15% of the isolates were able to secrete inhibitory substances against E. coli and Staphylococcus aureus. Molecular analysis by PCR amplification, sequencing of the 16S rRNA gene and BLAST bioinformatics analysis provides newly identified bacteria strains with new accession numbers in GenBank: Bacillus thuringiensis MBCBR322 (OP474008), Bacillus megaterium MBCBJ1822 (OP476493), Bacillus thuringiensis MBCBR222 (OP476494), Priestia megaterium MBCBJ2022 (OP476495) and Lactobacillus paraplantarum MBCBR1522 (OP476496). Multiple sequences alignment of identified sequences with their homologs of GenBank has shown high similarities. The phylogenetic inference assay has provided the two groups of strains observed in this study, and the two groups are very coherent with the phylogeny of the reference. 展开更多
关键词 Antimicrobial activity Hydrolytic activity Sequencing PEPPER BRAZZAVILLE
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Superwetting Ag/α-Fe_(2)O_(3) anchored mesh with enhanced photocatalytic and antibacterial activities for efficient water purification 被引量:1
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作者 Jiakai Li Changpeng Lv +5 位作者 Jiajia Song Xiaoling Zhang Xizhen Huang Yingzhuo Ma Haijie Cao Na Liu 《Green Energy & Environment》 SCIE EI CAS CSCD 2024年第1期89-103,共15页
Superwetting materials have drawn unprecedented attention in the treatment of oily wastewater due to their preferable anti-fouling property and selective oil/water separation.However,it is still a challenge to fabrica... Superwetting materials have drawn unprecedented attention in the treatment of oily wastewater due to their preferable anti-fouling property and selective oil/water separation.However,it is still a challenge to fabricate multifunctional and environmentally friendly materials,which can be stably applied to purify the actual complicated wastewater.Here,a Ag/Ag/α-Fe_(2)O_(3) heterostructure anchored copper mesh was intentionally synthesized using a facile two-step hydrothermal method.The resultant mesh with superhydrophilicity and underwater superoleophobicity was capable of separating various oil/water mixtures with superior separation efficiency and high permeationflux driven by gravity.Benefiting from the joint effects of the smaller band gap of Ag/α-Fe_(2)O_(3) heterojunction,inherent antibacterial capacity of Ag/α-Fe_(2)O_(3) and Ag nanoparticles,favorable conductive substrate,as well as the hierarchical structure with superwettability,such mesh presented remarkably enhanced degradation capability toward organic dyes under visible light irradiation and antibacterial activity against both Escherichia coli(E.coli)and Staphylococcus aureus(S.aureus)compared with the pure Ag/α-Fe_(2)O_(3) coated mesh.Impressively,the mesh exhibited bifunctional water purification performance,in which organic dyes were eliminated simultaneously from water during oil/water separation in onefiltration process.More importantly,this mesh behaved exceptional chemical resistance,mechanical stability and long-term reusability.Therefore,this material with multifunctional integration may hold promising potential for steady water purification in practice. 展开更多
关键词 Superwetting Ag/α-Fe_(2)O_(3)heterostructure Enhanced photocatalytic and antibacterial activities Water purification Long-term reusability
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Semisynthetic Derivatives of Sissotrin Isolated from Trifolium baccarinii Chiov. (Fabaceae) and Evaluation of Their Antibacterial Activities
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作者 Donald Léonel Feugap Tsamo Cyrille Ngoufack Tagousop +2 位作者 Arnaud Joseph Nguetse Dongmo Laurence Voutquenne-Nazabadioko David Ngnokam 《Advances in Biological Chemistry》 CAS 2024年第1期27-39,共13页
Starting from sissotrin (1), a natural isoflavonoid isolated from Trifolium baccarinii (Fabaceae), one new semisynthetic derivative, 6-nitrobiochanin A (1b) and two known derivatives, 8-nitrobiochanin A (1a) and 2&quo... Starting from sissotrin (1), a natural isoflavonoid isolated from Trifolium baccarinii (Fabaceae), one new semisynthetic derivative, 6-nitrobiochanin A (1b) and two known derivatives, 8-nitrobiochanin A (1a) and 2",3",4",6"-tetraacetylsissotrin (1c) have been obtained after performing nitration and acetylation reactions. Their structures were assigned after interpretation of their spectrometric (HR-ESI-MS) and spectroscopic (NMR 1D and 2D) data and by comparison with those reported in the literature. The substrate as well as the semisynthetic derivatives were evaluated for their antibacterial activities against six strains. The results reveal that they are inactive or weakly active on the strains tested with the exception of 8-nitrobiochanin A (1a) which showed moderate activity (MIC = 62.5 μg∙mL<sup>−1</sup>) on Staphylococcus aureus ATCC 43300. 展开更多
关键词 Trifolium baccarinii ISOFLAVONOID NITRATION ACETYLATION Antibacterial activity
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Promoting public health through the 2024 Compendium of Physical Activities:Strategies for adults,older adults,and wheelchair users
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作者 Stephen D.Herrmann Scott A.Conger +1 位作者 Erik A.Willis Barbara E.Ainsworth 《Journal of Sport and Health Science》 SCIE CAS CSCD 2024年第6期739-742,共4页
Physical activity(PA)is fundamentally linked to public health,^(1)providing benefits including chronic disease prevention and treatment,^(2)mental health,^(3)musculoskeletal health,^(4)and healthy aging,^(5)along with... Physical activity(PA)is fundamentally linked to public health,^(1)providing benefits including chronic disease prevention and treatment,^(2)mental health,^(3)musculoskeletal health,^(4)and healthy aging,^(5)along with socioeconomic advantages.^(6)These benefits highlight the importance of promoting PA for all populations.The recent publication of the Compendium of Physical Activities(Compendium)serves as a valuable resource,^(7)offering an updated and expanded guide for adults(ages 19-59)with tailored energy cost values for diverse populations. 展开更多
关键词 activities PREVENTION offering
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The influence of Lactobacillus plantarum fermentation in selenium-enriched Brassica napus L.:changes in the nutritional constituents,bioactivities and bioaccessibility
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作者 Wen Wang Zhixiong He +3 位作者 Ruiying Zhang Min Li Zhenxia Xu Xia Xiang 《Oil Crop Science》 CSCD 2024年第2期81-90,共10页
Selenium(Se)-enriched Brassica napus L.is a valuable organic Se supplement.In this study,the fermentation broth enriched with organic Se(FFS)was prepared using Lactobacillus plantarum to ferment the substrate of Se-en... Selenium(Se)-enriched Brassica napus L.is a valuable organic Se supplement.In this study,the fermentation broth enriched with organic Se(FFS)was prepared using Lactobacillus plantarum to ferment the substrate of Se-enriched Brassica napus L.Significant increases were observed after fermentation in total sugars,reducing sugars,soluble proteins,total phenolic content(TPC),and total flavonoid content(TFC).The organic Se was retained at a concentration of 54.75 mg/g in the freeze-dried sample.Principal component analysis and cluster analysis showed good separation between the FFS and unfermented(FS)groups.Fragrant 2-ethyloxetane had the highest content among all volatiles,while sinapine had the highest content among all phenolic compounds.The fermentation process showed remarkable improvement in the abundance and concentration of volatile compounds and phenolic contents,making FFS exhibit strong antioxidant activity and inhibitory capacity againstα-glucosidase activity.The bioaccessibility of phenolic compounds was significantly greater in FFS compared to FS.ADMET analysis revealed that the majority of phenolic compounds contained in FFS did not exhibit mutagenicity toxicity,hepatotoxicity,skin sensitization,or blood-brain barrier penetration,indicating a favorable level of biosafety.Overall,our study provides a new insight into the further utilization of Se-enriched Brassica napus L.in foods. 展开更多
关键词 Se-enriched Brassica napus L. FERMENTATION Antioxidant activity Inhibitory activity BIOACCESSIBILITY
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Three previously undescribed steroidal glycoalkaloids from Solanum lyratum and their anti-tumor activities 被引量:1
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作者 Liangyu Liu Yuke Yang +2 位作者 Xiao Du Tong Wu Jiannong Wang 《Journal of Chinese Pharmaceutical Sciences》 CAS CSCD 2022年第3期192-201,共10页
In the present study,three previously undescribed steroidal glycoalkaloids(compounds 1–3)were isolated from Solanum lyratum.Their structures were elucidated based on comprehensive spectroscopic data.Their anti-angiog... In the present study,three previously undescribed steroidal glycoalkaloids(compounds 1–3)were isolated from Solanum lyratum.Their structures were elucidated based on comprehensive spectroscopic data.Their anti-angiogenesis and anti-metastatic activities were evaluated by MTT and wound-healing assays,respectively.Tumor-derived vascular endothelial cells(TdECs),obtained by co-culture of A549 and human umbilical vein endothelial cells(HUVECs),were treated with compounds 1–3.Results showed that compounds 1–3 significantly inhibited the migration of TdECs at 25μM despite the weak cytotoxic activities,which indicated that the compounds exerted anti-tumor activities by inhibiting metastasis,rather than directly inhibiting the proliferation of TdECs. 展开更多
关键词 Solanum lyratum Steroidal glycoalkaloids anti-tumor METASTASIS ANGIOGENESIS
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