Evaluation of Anti-Wrinkle, Relieving Effect and Safety of A Group of Hexapeptide Compounds

The anti-wrinkle,relieving effect and safety of a group of hexapeptide compounds(HCs)were evaluated by in vitro antioxidant test,hyaluronidase inhibition test and human clinical test.The results showed that HCs possess+high overall antioxidant activity,and the scavenging activity of ABTS•radical and DPPH radical by 4 mg/mL HCs solution were(92.23±1.15)%and(90.24±1.25)%.The scavenging activity of PTIO radical by 80 mg/mL HCs solution was(80.29±2.41)%.The inhibition rate of 80 mg/mL HCs on hyaluronidase was(75.00±6.13)%.The test of clinical results showed that the HCs have good anti-wrinkle and relieving effect,and no case of grade 1 or above skin adverse reactions occurred,indicating that HCs have good safety.

The efficacy study of trinity permeation synergism on anti-aging

Background:More and more consumers are paying attention to skin rejuvenation.However,there is a lack of a non-invasive and efficient solution.Objective:To evaluate the efficacy of a trinity permeation synergism(TPS),which consists of a firming essence,an atomizer and a photoelectric penetrator,for facial anti-aging efficacy.Material and methods:In this work,in vitro cell experiments and human efficacy study were used to evaluate the firming and anti-wrinkle effects.Cell experiments were used to verify the effect of the firming essence on the cell proliferation,migration,and anti-inflammation in keratinocytes(HaCaT),and on the gene expression levels of type I and type III collagen(Col-1 and Col-3)and type I matrix metalloproteinase(MMP-1)in human skin fibroblasts(HSF).After in vitro test,60 women aged 35–60 years were enrolled in the randomized test,of which 30 subjects were randomly selected to be the experimental group and treated with the TPS system,while the left 30 subjects were treated with the firming essence only considered as control.After 28 days,skin elasticity,skin redness value,and skin wrinkles were measured to evaluate the efficacy of the TPS system.Results:Cell experiments showed that the firming essence can significantly improve the proliferation and the migration of HaCaT cells.It also promoted the expression level of Col-1 and Col-3 gene,and inhibited the expression level of MMP-1 gene in HSF cells.After confirming the efficacy of firming essence,the efficacy benefit of the TPS was further studied.The 28-day tests show that combined use firming essence with atomizer and penetrator can significantly increase skin elasticity,reduce skin hemoglobin value and skin wrinkles on Day 28.Moreover,all the mentioned improvements are significantly better than that in the control group.Conclusion:Through efficient delivery in the whole process,TPS boosts the efficacy of active components in the firming essence.TPS offers an efficient,non-invasive,and convenient way for enhanced facial rejuvenation efficacy.

Sleeping on an Anti-Wrinkle Pillow Reduces Facial Wrinkles: Results from an Anatomical Study

Background: A special pillow was designed to redistribute mechanical stress during sleeping in order to slow down the formation of facial skin wrinkles. Objective: To investigate whether sleeping on a specially designed pillow reduces facial skin wrinkles. Participants and Methods: A 28-day pilot study was carried out in which fifteen healthy female volunteers aged 23 - 55 years (mean age 35. 6 ± 8.5) slept on an antiwrinkle pillow. Evaluation of facial wrinkles was conducted before commencing the study (T0), following at 14 days (T14), and at 28 days (T28) when the study ended. Wrinkle density was assessed by computerized analysis of 2D images of participants’ faces. Results: A statistically significant decrease in wrinkle density was detected while smiling around both eyes, around the right eye in a relaxed facial expression, on average in all observed facial areas, around the left periorbital area in participants who predominantly slept on their left side of the body, but not on the frontal area. Limitations: A 3D camera could be used to better visualize and analyze wrinkle density. Conclusions: Sleeping on the specially designed pillow reduces facial wrinkles.

Anti-senescence and anti-wrinkle activities of 3-bromo-4,5-dihydroxybenzaldehyde from Polysiphonia morrowii Harvey in human dermal fibroblasts

Objective:To investigate the anti-senescence effect of 3-bromo-4,5-dihydroxybenzaldehyde(BDB)from Polysiphonia morrowii Harvey in human dermal fibroblasts(HDF).Methods:HDF were subjected to treatment of BDB and then treated with hydrogen peroxide(H2O2)to induce premature senescence.Senescence-associatedβ-galactosidase(SA-β-gal)activity in HDF was determined using the SA-β-gal staining method.Intracellular reactive oxygen species(ROS)production was measured using the 2’,7’-dichlorodihydrofluorescein diacetate assay.Western blotting assay was performed to assess the level of antioxidant enzyme glutathione peroxidase 1(GPX1).In addition,intracellular collagen and collagenase contents were analyzed using the respective ELISA kits.Elastase activity in HDF supernatants was measured from p-nitroaniline release and normalized using total protein content.Results:Treatment of HDF with H2O2 increased the activity of SA-β-gal,but BDB pre-treatment resulted in the reduction of SA-β-gal activity.Moreover,BDB significantly reduced H2O2-induced intracellular ROS production.BDB also markedly increased the level of GPX1,which was inhibited by 400μM of H2O2.Furthermore,in in vitro study,BDB significantly increased intracellular collagen content and decreased matrix metalloproteinase-1 and elastase activities in HDF.Conclusions:Our results demonstrate that BDB shows antisenescence and anti-wrinkle activities in vitro.

Anti-wrinkle Finishing of Cotton Fabrics with Pyromellitic Acid Enhanced by Polyol Extenders

Pyromellitic acid(PMA)has been employed for formaldehyde-free anti-wrinkle finishing on cotton fabrics for its low damage to fibers and absence of phosphorus-containing catalysts.However,the wrinkle resistance performance of fabrics failed to meet the application expectation.In this work,the polyol extenders were introduced into PMA cross-linked cellulose for further improving fabric resilience by a two-step dipping-squeezing-curing method.Eleven polyols were employed as the chain extenders for PMA anti-wrinkle finishing on fabrics,among which sorbitol presented the best chain-spreading effect on wrinkle resistance.After screening the process parameters,the optimum process was obtained as follows.The dosage ratio of PMA to sorbitol was 1∶1,the curing condition was 150-160℃ for 2-3 min.Scanning electron microscopy(SEM),thermogravimetric analysis(TGA)and Fourier transform infrared(FTIR)spectroscopy were employed to characterize the fiber changes during the process.The results showed that the sorbitol incorporation could facilitate the remelting and redissolution of PMA on fiber surface.During the curing process,sorbitol could form ester linkages between PMA molecules and improve the fiber resilience.The treated samples presented excellent washing durability,and the wrinkle recovery angle A_(w)was still 265.1°after 10 washes,which was close to that of 1,2,3,4-butane tetracarboxylic acid(BTCA)but with lower tearing load loss.The results provide new insights for the application of PMA in formaldehyde-free anti-wrinkle finishing on cotton fabrics.

Study of a Whitening and Anti-wrinkle Bio-functional Ingredient Based on Epigenetics

Via quantitative polymerase chain reaction （qPCR） test method, the efficacy of hexapeptide-2 was studied, including silencing on miR-29 and increasing miR-218 expression which targeted collagen coding mRNA （message RNA） then decreases the collagen synthesis, and the latter silences the melanin synthesis related enzyme. Then the double blind spilt-face clinical test on 35 Asian people （18 male and 17 female） was conducted to evaluate the whitening and anti-wrinkle effect. The qPCR test showed that hexapeptide-2 can decrease miR-29 expression for human fibroblast of different age （P 〈 0.05）; and the result on human melanocyte showed that it can increase miR-218 expression （P 〈 0.05）. Immunostaining results showed that it can increase the expression of collagen I and III significantly （P 〈 0.05）. The double blind split - face test showed that 1% hexapeptide-2 displays significant efficacy for skin whitening, spots lightening, skin erythema and skin dullness improvement, as well as skin wrinkles reduction as comparing with that from the placebo side （P 〈 0.05）.

Skin Retention Determination and Clinical Efficacy Evaluation of a Novel High Oil-Contained Hpr Transfersome Permeable Formulation

In order to increase the skin retention of HPR(Hydroxypinacone Retinoate),a novel high oil-contained HPR transfersome WL-HPR1130 was prepared.Conventional essence containing HPR was used as the control group,WL-HPR1130 essence was used as the experimental group,the skin retention of HPR were determined respectively.The anti-wrinkle efficacy of WL-HPR1130 essence was evaluated at the same time.The retention of HPR was 11.40μg/cm2 in the experimental group and 1.41μg/cm2 in the control group,which was 8.08 times higher than the control group.The wrinkles and fine lines were significantly reduced and smoothed after 2 and 4 weeks by experimental group sample.The average length,width and depth of wrinkles were decreased by 14.26%,18.31%,7.17%after 2 weeks,and were decreased by 29.39%,38.25%,8.60%after 4 weeks.The average length,width and depth of fine lines were decreased by 22.35%,12.60%,8.00%after 2 weeks,and were decreased by 27.10%,20.24%and 15.90%after 4 weeks,which has a significant visual changes.WLHPR1130,a novel high oil-contained HPR transfersome formulation,can significantly increase the retention of HPR in the skin.And the WL-HPR1130 essence have remarkable anti-wrinkle and smooth fine lines effects.

Quality Life Drives Laundry Care Update

This paper briefly introduces the current laundry care market and the overall development trend in recent years,discusses in detail the four most popular laundry care claims in the market,analyzes the overall upgrading of the laundry care market,and looks forward to the future prospects.

The Present Studies on Acmella Oleracea Extract

Considering the present article and patents,this article means to show the study and application of Acmella Oleracea extract in cosmetic field.Now studies show that the main actives is Alkyl amide,especially Spilanthol.Spilanthol is widely recognized as the most important active ingredient.Patents about Acmella Oleracea Extract mainly focus on anti-wrinkle effects and the anti-wrinkle effects when combine with other famous ingredient.Study also point out that ethanol extract may contain more active ingredient.Give prospective views on the application of Acmella Oleracea extract.

The Neglected Importance of Sleep on the Formation and Aggravation of Facial Wrinkles and Their Prevention

The duration of sleep and the position of the face while resting on a pillow have a negative impact on the facial skin appearance and may lead to the formation of sleep wrinkles. Sleep lines occur when there is repetitive, long-term tension on the facial skin, which pushes or pulls the skin in a direction that is perpendicular to the direction of the muscles of the face. These lines tend to be more vertically oriented than expression lines and can be found on the forehead, around the eyebrows, the eyes, the cheeks, the chin, and the nasolabial folds. Our studies revealed that the average reduction of wrinkles in total investigated area of the face (expressed as the density of wrinkle per surface skin) was approximately 12% after 28 days of sleep on a specially-designed pillow. The specially designed anti-wrinkle pillows eliminate the pressure on the cheeks, the eyes and the mouth during sleep. Many such pillows have been designed to reduce the aging process and to encourage users to sleep in specific positions. Evidence supporting the claim that a special pillow prevents wrinkles was presented. Nevertheless, prolonged human studies are required to further elucidate the role of sleeping on appearance of facial wrinkles.